62 results on '"Ma, Lie"'
Search Results
2. A new cleistanthane diterpenoid from the cultures of a mutant of Calcarisporium arbuscula NRRL 3705.
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Lu, Hui, Chen, Hong-Yu, Luo, Shan-Rong, Guo, Lu, Luo, Di, Ma, Lie-Feng, and Zhan, Zha-Jun
- Abstract
Calcarispol A (1), a new cleistanthane diterpenoid bearing a rare oxetan-2-one moiety, was isolated from a culture of an aurovertins-null mutant of Calcarisporium arbuscula NRRL 3705, along with two known diterpenoids, 3α,5α,8β-trihydroxycleistanth-13(17),15-dien-18-oic acid (2) and calcarisporic acid J (3). The structure of 1 was elucidated by detailed interpretation of NMR and HR-ESI-MS data. Its absolute configuration was established by single-crystal X-ray diffraction analysis. Biological evaluation showed that 1 had marked cytotoxicity against MCF-7 and HCT-116 cancer cells. [Display omitted] • A new diterpenoid bearing a rare oxetan-2-one moiety was obtained from the mutant. • The new diterpenoid displayed marked cytotoxicity against two cancer cell lines. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Minimally invasive delivery of human umbilical cord-derived mesenchymal stem cells by an injectable hydrogel via Diels–Alder click reaction for the treatment of intrauterine adhesions.
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Hu, Sentao, Dai, Yangyang, Xin, Liaobing, Zheng, Xiaowen, Ye, Zi, Zhang, Songying, and Ma, Lie
- Subjects
ENDOMETRIUM ,TISSUE adhesions ,MESENCHYMAL stem cells ,DIELS-Alder reaction ,HYDROGELS ,MINIMALLY invasive procedures ,PLANT fertility - Abstract
Intrauterine adhesions (IUA) are the most common cause of uterine infertility, and conventional treatments have not consistently achieved satisfactory pregnancy rates. Stem cell therapy shows promising potential for the clinical treatment of IUA. Although various advanced biomaterials have been designed for delivering stem cells to the uterine cavity, there remain significant challenges, particularly in devising therapeutic strategies for clinical application that minimize surgical incisions and conform to the intricate structure of uterine cavity. Herein, an injectable hydrogel loaded with human umbilical cord-derived mesenchymal stem cells (UCMSCs) was synthesized via the Diels-Alder click reaction for endometrial regeneration and fertility restoration, exhibiting suitable mechanical properties, good biocompatibility, and desirable degradation properties. Notably, this hydrogel permitted minimally invasive administration and integrated seamlessly with surrounding tissue. Our study revealed that the UCMSCs-laden injectable hydrogel enhanced cell proliferation, migration, angiogenesis, and exhibited anti-fibrotic effects in vitro. The implantation of this hydrogel significantly facilitated endometrium regeneration and restored fertility in a rat endometrial damage model. Mechanistically, in vivo results indicated that the UCMSCs-laden injectable hydrogel effectively promoted macrophage recruitment and facilitated M2 phenotype polarization. Collectively, this hydrogel demonstrated efficacy in regenerating damaged endometrium, leading to the restoration of fertility. Consequently, it holds promise as a potential therapeutic strategy for endometrial damage and fertility decline arising from intrauterine adhesions. Severe endometrial traumas frequently lead to intrauterine adhesions and subsequent infertility. Stem cell therapy shows promising potential for the clinical treatment of IUA; however, challenges remain, including low delivery efficiency and compromised stem cell activity during the delivery process. In this study, we fabricated an injectable hydrogel loaded with UCMSCs via the Diels-Alder click reaction, which exhibited unique bioorthogonality. The in situ-gelling hydrogels could be introduced through a minimally invasive procedure and adapt to the intricate anatomy of the uterus. The UCMSCs-laden injectable hydrogel promoted endometrial regeneration and fertility restoration in a rat endometrial damage model, efficaciously augmenting macrophage recruitment and promoting their polarization to the M2 phenotype. The administration of UCMSCs-laden injectable hydrogel presents a promising therapeutic strategy for patients with severe intrauterine adhesion. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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4. Construction of a Soft Antifouling PAA/PSBMA Hydrogel Coating with High Toughness and Low Swelling through the Dynamic Coordination Bonding Provided by Al(OH)3 Nanoparticles.
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Hu, Jinpeng, Zhang, Dezhi, Li, Wenbao, Li, Yan, Shan, Guorong, Zuo, Min, Song, Yihu, Wu, Ziliang, Ma, Lie, Zheng, Qiang, and Du, Miao
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- 2024
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5. A conductive multifunctional hydrogel dressing with the synergistic effect of ROS-scavenging and electroactivity for the treatment and sensing of chronic diabetic wounds.
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Zhang, Haiqi, Hu, Hongtao, Dai, Yangyang, Xin, Liaobing, Pang, Qian, Zhang, Songying, and Ma, Lie
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CHRONIC wounds & injuries ,HYDROCOLLOID surgical dressings ,HUMAN mechanics ,DEXTRAN ,REACTIVE oxygen species ,ELECTRIC stimulation ,HYDROGELS ,POLYMER networks - Abstract
Chronic diabetic wound with persistent inflammatory responses is still a serious threat to human health and life. Ideal wound dressings can be applied not only for covering the injury area, but also for regulating the inflammation to accelerate the wound healing and long-term monitoring of wound condition. However, there remains a challenge to design a multifunctional wound dressing for simultaneous treatment and monitoring of wound. Herein, an ionic conductive hydrogel with intrinsic reactive oxygen species (ROS)-scavenging properties and good electroactivity was developed for achieving the synergetic treatment and monitoring of diabetic wounds. In this study, we modified dextran methacrylate with phenylboronic acid (PBA) to prepare a ROS-scavenging material (DMP). Then the hydrogel was constructed by phenylboronic ester bonds induced dynamic crosslinking network, photo-crosslinked DMP and choline-based ionic liquid as the second network, and the crystallized polyvinyl alcohol as the third network, realizing good ROS-scavenging performance, high electroactivity, durable mechanical properties, and favorable biocompatibility. In vivo results showed that the hydrogel combined with electrical stimulation (ES) demonstrated good performance in promoting re-epithelization, angiogenesis and collagen deposition in chronic diabetic wound treatment by alleviating inflammation. Notably, with desirable mechanical properties and conductivity, the hydrogel could also precisely monitor movements of human body and possible tensile and compressive stresses of the wound site, providing timely alerts of excessive mechanical stress applied to the wound tissue. Thus, this "all-in-one" hydrogel exhibits great potential in constructing the next generation flexible bioelectronics for wound treatment and monitoring. Chronic diabetic wounds characterized by overexpressed reactive oxygen species (ROS) are still a serious threat to human health and life. However, there remains a challenge to design a multifunctional wound dressing for simultaneous wound treatment and monitoring. Herein, a flexible conductive hydrogel dressing with intrinsic ROS-scavenging properties and electroactivity was developed for the combined treatment and monitoring of the wound. The antioxidant hydrogel combined with electrical stimulation synergistically accelerated chronic diabetic wound healing by regulating oxidative stress, alleviating inflammation, promoting re-epithelization, angiogenesis and collagen deposition. Notably, with desirable mechanical properties and conductivity, the hydrogel also presented great potential in monitoring possible stresses of the wound site. The "all-in-one" bioelectronics integrating the treatment and monitoring functions present great application potential for accelerating chronic wound healing. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2023
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6. Neuroprotective carbazole alkaloids from Streptomyces sp. HS-NF-1322 by inhibition of ferroptosis.
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Peng, Yuan, Luo, Yi-Tong, Liu, Qian-Qing, Lou, Si-Qing, Liang, Dong-E., Zhan, Zha-Jun, Xi, Zhi-Fang, and Ma, Lie-Feng
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Three rare new carbazole alkaloids, antiostatin A 7 (1), (±)-morindolestatin B (2) and morindolestatin C (3), were obtained from a fermentation broth of the soil-derived Streptomyces sp. HS-NF-1322 strain. Their structures were assigned by various spectroscopic techniques including HR-ESI-MS and
2 D NMR. These new isolates, together with (±)-morindolestatin (4), were assayed for their neuroprotective effects on erastin-induced ferroptosis on the PC12 and HT-22 cell lines. All of these alkaloids significantly improved cell viability, and reduced ROS production of these neuronal cells. Notably, compound 1 dramatically protected PC12 cells from ferroptosis with a higher viability than the positive drug deferoxamine at 10 μM. [Display omitted] • Further chemical investigation on Streptomyces sp. HS-NF-1322. • Isolation of three rare carbazole alkaloids, antiostatin A 7 , morindolestatin B and C. • The first report on neuroprotective activity of antiostatin A and morindolestatin by inhibition of ferroptosis. • Antiostatin A 7 could significantly inhibit ferroptosis on PC12 cells with an EC 50 of 0.06 μM. [ABSTRACT FROM AUTHOR]- Published
- 2023
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7. Neuroprotective flavonoids from Epimedium brevicornu by inhibition of ferroptosis.
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Chen, Yu-Chen, Zheng, Qiong, Lu, Hui, Zhuang, Yi-Xin, Gong, Ting-Ting, Ma, Lie-Feng, and Zhan, Zha-Jun
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A new flavonoid glycoside bearing an unusual furan ring, epimedokoreanin F (1), was isolated from Epimedium brevicornu , along with 17 known flavonoids (2−18). The structures of these flavonoids were identified based on interpretation of spectroscopic data and comparison with literature data. Biologically, compounds 1−4 exhibited significant neuroprotective effects on the model of RSL3-induced ferroptosis in HT22 cells. Among them, compound 1 displayed the best activity with EC 50 values of 0.95 μ M, and can be used as lead compound for the development of neuroprotective agent to treat neurological diseases. [Display omitted] • 18 flavonoids including a new flavonoid were identified from E. brevicornu. • Four flavonoids exhibited significant neuroprotective effects. • Epimedokoreanin F has potential as neuroprotective agent by inhibition of ferroptosis. [ABSTRACT FROM AUTHOR]
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- 2023
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8. The importance of temperature monitoring in predicting wound healing
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Zhong, Yu-Fan, Wang, Zheng-Cai, Xue, Ya-Nan, Zhao, Wan-Yi, Liu, Yan-Qi, Wang, Xiao-Feng, Hu, Yan-Yan, Fang, Qing-Qing, Ma, Lie, Wang, Xiao-Zhi, Lou, Dong, and Tan, Wei-Qiang
- Abstract
The formation and healing of wounds are often accompanied by changes of various indexes, such as temperature, humidity, pressure, pH and exudate composition in the wound microenvironment. Monitoring these changes during the wound healing process is important as it helps in the early detection of abnormal healing, and promotes the implementation of suitable and efficient interventions. Of all the measurable characteristics of wounds, wound temperature is the most reliable index to observe or measure, as temperature changes and trends during the wound healing process vary in different types of wounds. Therefore, the measurement and analysis of wound temperature changes may assist in judging wound status, contributing to the development of treatment plans, and may predict wound healing outcomes. There are two methods of measuring temperature: single point monitoring and remote monitoring, and two comparison methods for measurement data: temperature difference to the same site of the skin opposite and to periwound skin. The focus of this review is to discuss the significant relationship between wound temperature and wound healing, and to evaluate the value of temperature monitoring in predicting wound healing. Local heating treatments, such as thermal radiation dressings and water-filtered infrared-A, and their use in promoting healing in acute and hard-to-heal wounds are also discussed.Conclusion:Upon wound formation, the wound temperature rises in the first 3–4 days until reaching its peak. It then falls at about one week after wound formation. In the second week after wound formation, the wound temperature decreases steadily to the baseline indicating a good wound condition and progression towards healing. While a continuous high temperature is often a sign of excessive inflammation or infection, which indicates urgent need of intervention and treatment.
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- 2023
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9. Inflammatory environment-adaptive patterned surface for spatiotemporal immunomodulation of macrophages.
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Luo, Yilun, Yuan, Peiqi, Hu, Sentao, Wang, Hanwen, Zhang, Haiqi, and Ma, Lie
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IMMUNOREGULATION ,MACROPHAGES ,HEALING ,PSYCHOLOGICAL feedback ,HYALURONIC acid - Abstract
Designing biomaterials with precise immunomodulation can help to decipher the dynamic interactions between macrophages and biomaterials to match the tissue healing process. Although some advanced stimuli-responsive immunomodulatory biomaterials were reported for cell dynamic modulation, while most triggers need external stimuli by manual intervention, there would be the inevitable errors and uncertainties. Thus, developing immunomodulatory biomaterials with adaptive abilities, which can recognize the inflammation signals, change their properties spatiotemporally under the microenvironment triggers, and provide feedback to realize macrophages modulation in different healing stages, has become a promising strategy. In this work, we developed an inflammation-adaptive Arg-Gly-Asp (RGD) -patterned surface for spatiotemporal immunomodulation of macrophage. We fabricated a methacrylated hyaluronic acid (MA-HA) hydrogel with thiol-functionalized RGD-patterned surface by employing photolithography technology. Then, thiol-functionalized RGD contained ROS-cleavable linker was filled the remaining sites and consequently, a dynamic surface with temporary homogeneous RGD was obtained. Under the overproduction of ROS by the inflammation-activated macrophages, the linker was cleaved, and the homogeneous RGD surface was transformed to the RGD patterned surface, which triggered elongation of macrophages and consequently the upregulated expressions of arginase-1, IL-10 and TNF-β1, indicating the polarization toward to anti-inflammatory phenotype. Developing inflammatory environment-adaptive surface for spatiotemporal modulation of macrophages polarization provides a precise and smart strategy for the healing-matched immunomodulation to facilitate healing outcomes. Designing biomaterials with precise immunomodulation can help to decipher the dynamic interactions between macrophages and biomaterials to match tissue repair process. Some immunomodulatory biomaterials were reported for cell dynamic modulation, while most triggers need external manual intervention. Thus, we developed an immunomodulatory biomaterial with inflammation-adaptive patterned surface, which can recognize abnormal signals and change its properties spatiotemporally under the microenvironment triggers, and provide feedback to realize macrophages modulation in different stages. The dynamic surface can adapt to the changes of microenvironment and dynamically to match the cell behavior and tissue healing process on demand without external manual intervention. Additionally, the surface achieves the balance of macrophages with pro- and anti-inflammatory phenotypes in the tissue repair process. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2022
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10. NIR‐triggered hydrogel with dynamic stiffness via ion chelation to modulate macrophage phenotypes
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Yuan, Peiqi, Luo, Yilun, and Ma, Lie
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The immune system plays a central role in tissue repair and regeneration. Macrophages have emerged as a primary target because of their critical roles in regulating multiple phases of tissue repair through their unique plasticity and ability to rapidly shift phenotypes. Smart biomaterials, which able to harness macrophage phenotypes on demand during the healing process, have become a promising strategy. Here, a novel integrated hydrogel with near‐infrared (NIR) responsive dynamic stiffness was conducted, and found sequentially polarizing bone marrow‐derived macrophages from pro‐inflammatory to anti‐inflammatory phenotype in situ. Under NIR irradiation, ethylene diamine tetraacetic acid released from the IR780‐mixed phase change material into the hydrogel, resulting in the decrease of hydrogel stiffness in situ by chelating the calcium ion (Ca2+) in the calcium alginate hydrogel. The up‐regulation of arginase‐1 and interleukin‐10 were quantified by immunostaining and enzyme‐linked immune sorbent assay, respectively. The result indicated the transformation of macrophage from the pro‐inflammatory to anti‐inflammatory phenotype by dynamic stiffness drop. The modulation of macrophage phenotypes by stiffness‐degradation without the stimuli of cytokines offers an effective and noninvasive strategy to manipulate inflammatory or tissue‐regenerative immune responses to achieve optimized healing or therapeutical outcomes.
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- 2022
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11. Multi-objective topology optimization and flow characteristics study of the microfluidic reactor
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Wang, Jiahao, Wang, Yue, Ma, Lie, and Liu, Xiaomin
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An improved topology optimization method is proposed to optimize the performance and structure of the packed bed microfluidic reactor (PBMR). Dimensionless general scaling factor (GSF) is established to fully reflect the multi-physical properties of first-order isothermal catalytic reaction. Reaction performance and the flow loss are combined into a multi-objective function. Pareto algorithm is developed by introducing the weighted-sum method with weight factors ω, so Pareto frontier solution composed of optimal solutions under different ωis obtained to reveal the trade-off relationship between single-objective functions. Material density controlled by the improved polynomial function is adopted as the design variable to control the catalyst distribution. Gradient information of the design variable is updated by using the adjoint-based sensitivity analysis method, and Helmholtz filter method is adopted to solve the problems of grayscale, unclear interface, and channel fracture. The results demonstrate that the GSF can reflect the scaling behavior of the topology optimization reactor. Multi-objective functions under different ωare responded by changing the distribution of catalyst blocks and channel structure, which affects the diffusion and mixing of reactant. From the perspective of engineering application, this study provides a general and efficient new method for developing high-performance PBMRs.
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- 2022
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12. Temperature-Responsive Ionic Conductive Hydrogel for Strain and Temperature Sensors.
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Pang, Qian, Hu, Hongtao, Zhang, Haiqi, Qiao, Bianbian, and Ma, Lie
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- 2022
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13. Temperature-Responsive Ionic Conductive Hydrogel for Strain and Temperature Sensors
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Pang, Qian, Hu, Hongtao, Zhang, Haiqi, Qiao, Bianbian, and Ma, Lie
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Flexible wearable devices have achieved remarkable applications in health monitoring because of the advantages of multisignal collecting and real-time wireless transmission of information. However, the integration of bulky sensing elements and rigid metal circuit components in traditional wearable devices may lead to a mechanical and signal-conducting mismatch between wearable devices and biological tissues, thus restricting their wide applications in the human body. The excellent mechanical properties, conductivity, and high tissue resemblance of conductive hydrogel contribute to its application in flexible electronic sensors to monitor human health. In this work, a dual-network, temperature-responsive ionic conductive hydrogel with excellent stretchability, fast temperature responsiveness, and good conductivity was developed by introducing a polyvinylpyrrolidone (PVP)/ tannic acid (TA)/ Fe3+cross-linked network into the N,N-methylene diacrylamide (MBAA) cross-linked poly(N-isopropylacrylamide-co-acrylamide) (P(NIPAAm-co-AM)) network. Furthermore, the introduction of the PVP/TA/Fe3+cross-linked network endowed the hydrogel with excellent stretchability and conductivity. By adjusting the molar ratio of TA and Fe3+to 3:5, a hydrogel with a maximal stretching ratio of 720% and sensitive strain response (GF = 3.61) was achieved, showing a promising application in wearable strain sensors to monitor both large and fine human motions. Moreover, by introducing PNIPAAm with a lower critical solution temperature (LCST), the hydrogel may be used to monitor the environmental temperature through the temperature–conductivity responsiveness, which can be applied as a wearable temperature sensor to detect fever or tissue hyperthermia in the human body.
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- 2022
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14. Enhanced genome editing to ameliorate a genetic metabolic liver disease through co-delivery of adeno-associated virus receptor
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Yin, Shuming, Ma, Lie, Shao, Tingting, Zhang, Mei, Guan, Yuting, Wang, Liren, Hu, Yaqiang, Chen, Xi, Han, Honghui, Shen, Nan, Qiu, Wenjuan, Geng, Hongquan, Yu, Yongguo, Li, Shichang, Yu, Weishi, Liu, Mingyao, and Li, Dali
- Abstract
Genome editing through adeno-associated viral (AAV) vectors is a promising gene therapy strategy for various diseases, especially genetic disorders. However, homologous recombination (HR) efficiency is extremely low in adult animal models. We assumed that increasing AAV transduction efficiency could increase genome editing activity, especially HR efficiency, for in vivogene therapy. Firstly, a mouse phenylketonuria (PKU) model carrying a pathogenic R408W mutation in phenylalanine hydroxylase (Pah) was generated. Through co-delivery of the general AAV receptor (AAVR), we found that AAVR could dramatically increase AAV transduction efficiency in vitroand in vivo. Furthermore, co-delivery of SaCas9/sgRNA/donor templates with AAVR via AAV8 vectors increased indel rate over 2-fold and HR rate over 15-fold for the correction of the single mutation in PahR408Wmice. Moreover, AAVR co-injection successfully increased the site-specific insertion rate of a 1.4 kb PahcDNA by 11-fold, bringing the HR rate up to 7.3% without detectable global off-target effects. Insertion of PahcDNA significantly decreased the Phe level and ameliorated PKU symptoms. This study demonstrates a novel strategy to dramatically increase AAV transduction which substantially enhanced in vivogenome editing efficiency in adult animal models, showing clinical potential for both conventional and genome editing-based gene therapy.
- Published
- 2022
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15. New ingenane diterpenoids from Euphorbia kansui reverse multi-drug resistance.
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Chen, You, Luo, Di, Chen, Ning-Yu, Zhang, Yue, Liang, Dong-E, Zhan, Zha-Jun, and Ma, Lie-Feng
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[Display omitted] • The constituents of traditional Chinese medicine roots of Euphorbia kansui were investigated. • Two new ingenane diterpenoids along with eight known ones were isolated. • Kansuininol A and B showed the potential of MDR reverse against MCF-7/ADR. • It is the first example of ingenane as a MDR reversal agent. Ten ingenane-type diterpenoids, including two undescribed compounds named as kansuininols A–B (1 – 2), were isolated from the roots of Euphorbia kansui. Their structures were elucidated by detailed interpretation of their spectroscopic data, especially 2D-NMR and HR-ESI-MS. Chemoreversal assays of these isolates were carried on adriamycin resistant human breast adenocarcinoma cell line MCF-7/ADR, and compounds 1 – 2 were identified as potent low-cytotoxic MDR modulators with greater chemoreversal ability than the positive drug verapamilti. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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16. Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes
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Luo, Yilun, Zheng, Xiaowen, Yuan, Peiqi, Ye, Xingyao, and Ma, Lie
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Due to the critical roles of macrophage in immune response and tissue repair, harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many attentions. Although there have developed many advanced platforms with dynamic features for cell manipulation, few studies have designed a dynamic chemical pattern to sequentially polarize macrophage phenotypes and meet the immune requirements at various tissue repair stages. Here, we propose a novel strategy for spatiotemporal manipulation of macrophage phenotypes by a UV-induced dynamic Arg-Gly-Asp (RGD) pattern. By employing a photo-patterning technique and the specific interaction between cyclodextrin (CD) and azobenzene-RGD (Azo-RGD), we prepared a polyethylene glycol-dithiol/polyethylene glycol-norbornene (PEG-SH/PEG-Nor) hydrogel with dynamic RGD-patterned surface. After irradiation with 365-nm UV light, the homogeneous RGD surface was transformed to the RGD-patterned surface which induced morphological transformation of macrophages from round to elongated and subsequent phenotypic transition from pro-inflammation to anti-inflammation. The mechanism of phenotypic polarization induced by RGD pattern was proved to be related to Rho-associated protein kinase 2 (ROCK2). Sequential modulation of macrophage phenotypes by the dynamic RGD-patterned surface provides a remote and non-invasive strategy to manipulate immune reactions and achieve optimized healing outcomes.
- Published
- 2021
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17. Discovery of NO Donor-Aurovertin Hybrids as Dual Ferroptosis and Apoptosis Inducers for Treating Triple Negative Breast Cancer
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Ma, Lie-Feng, Xu, Li−Li, Yuan, Ling-Jie, Yang, Xi, Wu, Rui, Bao, Shu-Min, Chen, Yi-Li, Duan, Hong-Liang, Fang, Luo, Zhao, Hua-Jun, and Zhan, Zha-Jun
- Abstract
Triple-negative breast cancer (TNBC) is a highly lethal malignancy, and its clinical management encounters severe challenges due to its high metastatic propensity and the absence of effective therapeutic targets. To improve druggability of aurovertin B (AVB), a natural polyketide with a significant antiproliferative effect on TNBC, a series of NO donor/AVB hybrids were synthesized and tested for bioactivities. Among them, compound 4dsignificantly inhibited the proliferation and metastasis of TNBC in vitroand in vivowith better safety than that of AVB. The structure−activity relationship analysis suggested that the types of NO donor and the linkers had considerable effects on the activities. Mechanistic investigations unveiled that 4dinduced apoptosis and ferroptosis by the reduction of mitochondrial membrane potential and the down-regulation of GPX4, respectively. The antimetastatic effect of 4dwas associated with the upregulation of DUSP1. Overall, these compelling results underscore the tremendous potential of 4dfor treating TNBC.
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- 2024
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18. Construction of a Soft Antifouling PAA/PSBMA Hydrogel Coating with High Toughness and Low Swelling through the Dynamic Coordination Bonding Provided by Al(OH)3Nanoparticles
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Hu, Jinpeng, Zhang, Dezhi, Li, Wenbao, Li, Yan, Shan, Guorong, Zuo, Min, Song, Yihu, Wu, Ziliang, Ma, Lie, Zheng, Qiang, and Du, Miao
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Marine biofouling, resulting from the adhesion of marine organisms to ship surfaces, has long been a significant issue in the maritime industry. In this paper, we focused on utilizing soft and hydrophilic hydrogels as a potential approach for antifouling (AF) coatings. Acrylic acid (AA) with a polyelectrolyte effect and N-(3-sulfopropyl)-N-(methacryloxyethyl)-N,N-dimethylammonium betaine (SBMA) with an antipolyelectrolyte effect were selected as monomers. By adjusting the monomer ratio, we were able to create hydrogel coatings that exhibited low swelling ratio in both fresh water and seawater. The Al(OH)3nanoparticle, as a physical cross-linker, provided better mechanical properties (higher tensile strength and larger elongation at break) than the chemical cross-linker through the dynamic coordination bonds and plentiful hydrogen bonds. Additionally, we incorporated trehalose into the hydrogel, enabling the repair of the hydrogel network through covalent-like hydrogen bonding. The zwitterion compound SBMA endowed the hydrogel with excellent AF performance. It was found that the highest SBMA content did not lead to the best antibacterial performance, as bacterial adhesion quantity was also influenced by the charge of the hydrogel. The hydrogel with appropriate SBMA content being close to electrical neutrality exhibits the strongest zwitterionic property of PSBMA chains, resulting in the best antibacterial adhesion performance. Furthermore, the pronounced hydrophilicity of SBMA enhanced the lubrication of the hydrogel surface, thereby reducing the friction resistance when applied to the hull surface during ship navigation.
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- 2024
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19. A scaffold laden with mesenchymal stem cell-derived exosomes for promoting endometrium regeneration and fertility restoration through macrophage immunomodulation.
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Xin, Liaobing, Lin, Xiaona, Zhou, Feng, Li, Chao, Wang, Xiufen, Yu, Huaying, Pan, Yibin, Fei, Haiyi, Ma, Lie, and Zhang, Songying
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EXOSOMES ,ENDOMETRIUM ,MESENCHYMAL stem cells ,FERTILITY ,ESTROGEN receptors ,GREEN'S functions ,IMMUNOREGULATION - Abstract
Endometrial traumas may cause intrauterine adhesions (IUAs), leading to infertility. Conventional methods in clinic have not solved the problem of endometrial regeneration in severe cases. Umbilical cord-derived mesenchymal stem cell (UC-MSC)-based therapies have shown some promising achievements in the treatment of IUAs. However, the limitations of potential tumorigenicity, low infusion and low retention are still controversial and restricted the clinical application of MSCs. In contrast, UC-MSC-derived exosomes exhibit a similar function to their source cells and are expected to overcome these limitations. Therefore, a novel and viable cell-free therapeutic strategy by UC-MSC-derived exosomes was proposed in this study. Here, we designed a construct of exosomes and collagen scaffold (CS/Exos) for endometrial regeneration in a rat endometrium-damage model, and investigated the regeneration mechanism through macrophage immunomodulation. The CS/Exos transplantation potently induced (i) endometrium regeneration, (ii) collagen remodeling, (iii) increased the expression of the estrogen receptor α/progesterone receptor, and (iv) restored fertility. Mechanistically, CS/Exos facilitated CD163
+ M2 macrophage polarization, reduced inflammation, and increased anti-inflammatory responses in vivo and in vitro. By RNA-seq, miRNAs enriched in exosomes were the main mediator for exosomes-induced macrophage polarization. Overall, we demonstrated that CS/Exos treatment facilitated endometrium regeneration and fertility restoration by immunomodulatory functions of miRNAs. Our research highlights the therapeutic prospects of CS/Exos for the management of IUAs. Severe endometrial traumas always result in intrauterine adhesions (IUAs) and infertility. The limited outcomes by conventional methods in the clinic make it very important to develop new strategies for endometrium regeneration and fertility restoration. In this study, an exosome-laden scaffold (CS/Exos) was designed and the transplantation of CS/Exos potently induced (i) endometrium regeneration, (ii) collagen remodeling, (iii) increased the expression of the estrogen receptor α/progesterone receptor, and (iv) restored fertility. In mechanism, the construct of CS/Exos facilitated M2 macrophage polarization, reduced inflammation, and increased anti-inflammatory responses. Furthermore, miRNAs enriched in exosomes were the main mediator for exosome-induced macrophage polarization. This study highlights the therapeutic prospects of CS/Exos and the translational application for the management of severe IUAs. Image, graphical abstract [ABSTRACT FROM AUTHOR]- Published
- 2020
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20. SY67903 Produces Eunicellin Diterpenoids Structurally Similar to Terpenes of the Gorgonian , the Bacterial Source.
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Ma, Lie-Feng, Chen, Meng-Jia, Liang, Dong-E, Shi, Lin-Mei, Ying, You-Min, Shan, Wei-Guang, Li, Guo-Qiang, and Zhan, Zha-Jun
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- 2020
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21. Near-Infrared-Triggered Dynamic Surface Topography for Sequential Modulation of Macrophage Phenotypes.
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Zheng, Xiaowen, Xin, Liaobing, Luo, Yilun, Yang, Huang, Ye, Xingyao, Mao, Zhengwei, Zhang, Songying, Ma, Lie, and Gao, Changyou
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- 2019
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22. A “sandwich” cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypesElectronic supplementary information (ESI) available. See DOI: 10.1039/d0bm02194f
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Yuan, Peiqi, Luo, Yilun, Luo, Yu, and Ma, Lie
- Abstract
Considering the key roles of macrophages in tissue repair and immune therapy, designing smart biomaterials able to harness macrophage phenotypes on demand during the healing process has become a promising strategy. Here, a novel “sandwich” cell culture platform with near-infrared (NIR) responsive dynamic stiffness was fabricated to polarize bone marrow-derived macrophages (BMDMs) in situfor revealing the relationship between the macrophage phenotype and substrate stiffness dynamically. Under NIR irradiation, calcium ions (Ca2+) diffused through the middle layer of the IR780-mixed phase change material (PCM) due to the photothermal effect of IR780, resulting in an increase of hydrogel stiffness in situby the crosslinking of the upper layer of the hyaluronic acid-sodium alginate hydrogel (MA-HA&SA). The up-regulation of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α) was quantified by immunostaining and enzyme-linked immune sorbent assay (ELISA), respectively, indicating the transformation of macrophages from the anti-inflammatory to pro-inflammatory phenotype under dynamic stiffness. The nuclear Yes-associated-protein (YAP) ratio positively correlated with the shift of the macrophage phenotype. The modulation of macrophage phenotypes by stiffness-rise without the stimuli of cytokines offers an effective and noninvasive strategy to manipulate immune reactions to achieve optimized healing or therapeutic outcomes.
- Published
- 2021
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23. Gene-activated dermal equivalents to accelerate healing of diabetic chronic wounds by regulating inflammation and promoting angiogenesis
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Lou, Dong, Luo, Yu, Pang, Qian, Tan, Wei-Qiang, and Ma, Lie
- Abstract
Diabetic chronic wound, characterized by prolonged inflammation and impaired angiogenesis, has become one of the most serious challenges in clinic and pose a significant healthcare burden worldwide. Although a great variety of wound dressings have been developed, few of encouraged achievements were obtained so far. In this study, the gene-activated strategy was applied to enhance sustained expression of vascular endothelial growth factor (VEGF) and achieve better healing outcomes by regulating inflammation and promoting angiogenesis. The gene-activated bilayer dermal equivalents (Ga-BDEs), which has good biocompatibility, were fabricated by loading the nano-sized complexes of Lipofectamine 2000/plasmid DNA-encoding VEGF into a collagen-chitosan scaffold/silicone membrane bilayer dermal equivalent. The DNA complexes were released in a sustained manner and showed the effective transfection capacities to up-regulate the expression of VEGF in vitro. To overcome cutaneous contraction of rodents and mimic the wound healing mechanisms of the human, a reformative rat model of full-thickness diabetic chronic wound was adopted. Under the treatment of Ga-BDEs, speeding wound healing was observed, which is accompanied by the accelerated infiltration and phenotype shift of macrophages and enhanced angiogenesis in early and late healing phases, respectively. These proved that Ga-BDEs possess the functions of immunomodulation and pro-angiogenesis simultaneously. Subsequently, the better regeneration outcomes, including deposition of oriented collagen and fast reepithelialization, were achieved. All these results indicated that, being different from traditional pro-angiogenic concept, the up-regulated expression of VEGF by Ga-BDEs in a sustained manner shows versatile potentials for promoting the healing of diabetic chronic wounds.
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- 2020
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24. Streptomyces albogriseolusSY67903 Produces Eunicellin Diterpenoids Structurally Similar to Terpenes of the Gorgonian Muricella sibogae, the Bacterial Source
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Ma, Lie-Feng, Chen, Meng-Jia, Liang, Dong-E, Shi, Lin-Mei, Ying, You-Min, Shan, Wei-Guang, Li, Guo-Qiang, and Zhan, Zha-Jun
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Microeunicellols A (1) and B (2), two undescribed eunicellin diterpenoids, were isolated from the culture of a bacterial symbiont, Streptomyces albogriseolusSY67903. Their structures, including absolute configurations revealed by spectroscopic data and single-crystal X-ray diffraction analysis, are closely related with the diterpenoids from its host, a South China Sea gorgonian, Muricella sibogae. This is the first report of eunicellin diterpenoids, commonly coral-derived, from a bacterial symbiont of coral. The chemical metabolic relationship between the bacterium and its host is discussed. Biological evaluation revealed that compound 1possessed cytotoxicities against several human cancer cell lines.
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- 2020
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25. Morindolestatin, Naturally Occurring Dehydromorpholinocarbazole Alkaloid from Soil-Derived Bacterium of the Genus Streptomyces
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Zhang, Shao-Yong, Zhan, Zha-Jun, Zhang, Hui, Qi, Huan, Zhang, Li-Qin, Chen, Shi-Xin, Gan, Li-She, Wang, Ji-Dong, and Ma, Lie-Feng
- Abstract
Novel antilipid peroxidative carbazole alkaloids, antiostatin A5(1), antiostatin A6(2), and (±)-morindolestatin (3), were isolated from a new soil-derived Streptomycessp. Compound 2possesses an unusual cyclohexene side chain. Compound 3was a pair of enantiomers featuring an unprecedented [1,4]oxazino[2,3-c]carbazole ring system. The absolute configuration of 3was determined by online HPLC-ECD and ECD calculation. A racemization mechanism and putative biosynthetic pathway are discussed.
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- 2020
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26. Smart wound dressing for infection monitoring and NIR-triggered antibacterial treatmentElectronic supplementary information (ESI) available. See DOI: 10.1039/c9bm02060h
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QiaoThese authors contributed equally to this work., Bianbian, Pang, Qian, Yuan, Peiqi, Luo, Yilun, and Ma, Lie
- Abstract
Wound infection is a major challenge in the clinic that greatly hinders the wound healing process. It is highly important to develop smart wound dressings that can sense bacterial infection at early stages and provide on-demand treatment. In this work, a smart hydrogel-based wound dressing capable of monitoring bacterial infection viaa pH-responsive fluorescence resonance energy transfer (FRET) transition of Cyanine3 (Cy3) and Cyanine5 (Cy5) in a bacterial environment and providing on-demand treatment of infection vianear infrared (NIR) light-triggered antibiotic release was developed. The smart hydrogel was prepared by physical crosslinking of polyvinyl alcohol (PVA) and an ultraviolet (UV)-cleavable polyprodrug (GS-Linker-MPEG), in which Cy3 and Cy5-modified silica nanoparticles (SNP-Cy3/Cy5) were loaded and acted as a pH-responsive fluorescent probe to detect bacterial infection based on the FRET effect between Cy3 and Cy5. Also, up-conversion nanoparticles (UCNP) were loaded into the hydrogels to cleave the UV-responsive GS-Linker-MPEG and achieve NIR-responsive release of GS in the bacterial environment. The in vitrostudies proved that the smart hydrogels present good water absorption ability and excellent mechanical properties as well as good biocompatibility, which are necessary for their application in wound dressings. Moreover, the hydrogels showed obvious FRET transitions in both acidic buffer and bacteria solution. Upon irradiating the hydrogels with NIR light, UCNP were able to convert NIR light to UV light to trigger the release of GS from the hydrogels for antibacterial treatment. This research is expected to provide a new strategy for self-reporting and effective treatment of wound infection.
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- 2020
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27. A monomer-assembly template-directed synthesis of conjugated porous polymer microtubular bundlesElectronic supplementary information (ESI) available: Additional methods, schemes, tables and figures. See DOI: 10.1039/c9mh01381d
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Zhang, Wenbei, Cui, Tianlu, Bi, Shuai, Ma, Lie, Lu, Deng, Wu, Dongqing, and Zhang, Fan
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For porous organic materials, the defined morphology in the micrometre or a larger scale plays an extremely important role in fully exhibiting their molecular-level properties in the bulk phase, thus tuning the synergetic effects of different components and facilitating their fabrication and processing. However, the synthetic strategy towards porous organic materials with well-controlled morphologies is still very limited and full of challenges. Herein, we report a simple self-templated method to construct conjugated porous polymer microtubular bundles in the fused fashion. In the synthesis processes, the monomer 1,3,5-benzene-tricarbohydrazide is firstly assembled into crystalline microfiber bundles as the template, which is then condensed with 1,7-para-formylphenyl-perylene-3,4,9,10-tetracarboxylic diimide viathe dynamic covalent acylhydrazon linkage. Upon a quasi-Kirkendall effect and Ostwald ripening process, a conjugated porous polymer featuring smooth microtubular bundles can be essentially constructed. Such defined hierarchical morphology exhibits the substantial co-used tubular walls and orientated channels with sub-micrometre-sized diameters and lengths in tens of micrometers, thus rendering an extremely high accessibility of their redox active sites.
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- 2020
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28. Near-Infrared-Triggered Dynamic Surface Topography for Sequential Modulation of Macrophage Phenotypes
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Zheng, Xiaowen, Xin, Liaobing, Luo, Yilun, Yang, Huang, Ye, Xingyao, Mao, Zhengwei, Zhang, Songying, Ma, Lie, and Gao, Changyou
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Immune response is critical to tissue repair. Designing biomaterials with immunomodulatory functions has become a promising strategy to facilitate tissue repair. Considering the key roles of macrophages in tissue repair and the significance of the balance of M1 and M2, smart biomaterials, which can harness macrophage phenotypes dynamically to match the tissue healing process on demand, have attracted a lot of attention to be set apart from the traditional anti-inflammatory biomaterials. Here, we prepare a gold nanorod-contained shape memory polycaprolactone film with dynamic surface topography, which has the ability to be transformed from flat to microgrooved under near-infrared (NIR) irradiation. Based on the close relationships between the morphologies and the phenotypes of macrophages, the NIR-triggered surface transformation induces the elongation of macrophages, and consequently the upregulated expressions of arginase-1 and IL-10 in vitro, indicating the change of macrophage phenotypes. The sequential modulation of macrophage phenotypes by dynamic surface topography is further confirmed in an in vivo implantation test. The healing-matched modulation of macrophage phenotypes by dynamic surface topography without the stimuli of cytokines offers an effective and noninvasive strategy to manipulate tissue regenerative immune reactions to achieve optimized healing outcomes.
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- 2019
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29. Danger-associated extracellular ATP counters MDSC therapeutic efficacy in acute GVHD
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Koehn, Brent H., Saha, Asim, McDonald-Hyman, Cameron, Loschi, Michael, Thangavelu, Govindarajan, Ma, Lie, Zaiken, Michael, Dysthe, Josh, Krepps, Walker, Panthera, Jamie, Hippen, Keli, Jameson, Stephen C., Miller, Jeffrey S., Cooper, Matthew A., Farady, Christopher J., Iwawaki, Takao, Ting, Jenny P.-Y., Serody, Jonathan S., Murphy, William J., Hill, Geoffrey R., Murray, Peter J., Bronte, Vincenzo, Munn, David H., Zeiser, Robert, and Blazar, Bruce R.
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Myeloid-derived suppressor cells (MDSCs) can subdue inflammation. In mice with acute graft-versus-host disease (GVHD), donor MDSC infusion enhances survival that is only partial and transient because of MDSC inflammasome activation early posttransfer, resulting in differentiation and loss of suppressor function. Here we demonstrate that conditioning regimen–induced adenosine triphosphate (ATP) release is a primary driver of MDSC dysfunction through ATP receptor (P2x7R) engagement and NLR pyrin family domain 3 (NLRP3) inflammasome activation. P2x7R or NLRP3 knockout (KO) donor MDSCs provided significantly higher survival than wild-type (WT) MDSCs. Although in vivo pharmacologic targeting of NLRP3 or P2x7R promoted recipient survival, indicating in vivo biologic effects, no synergistic survival advantage was seen when combined with MDSCs. Because activated inflammasomes release mature interleukin-1ß (IL-1ß), we expected that IL-1ß KO donor MDSCs would be superior in subverting GVHD, but such MDSCs proved inferior relative to WT. IL-1ß release and IL-1 receptor expression was required for optimal MDSC function, and exogenous IL-1ß added to suppression assays that included MDSCs increased suppressor potency. These data indicate that prolonged systemic NLRP3 inflammasome inhibition and decreased IL-1ß could diminish survival in GVHD. However, loss of inflammasome activation and IL-1ß release restricted to MDSCs rather than systemic inhibition allowed non-MDSC IL-1ß signaling, improving survival. Extracellular ATP catalysis with peritransplant apyrase administered into the peritoneum, the ATP release site, synergized with WT MDSCs, as did regulatory T-cell infusion, which we showed reduced but did not eliminate MDSC inflammasome activation, as assessed with a novel inflammasome reporter strain. These findings will inform future clinical using MDSCs to decrease alloresponses in inflammatory environments.
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- 2019
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30. Danger-associated extracellular ATP counters MDSC therapeutic efficacy in acute GVHD
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Koehn, Brent H., Saha, Asim, McDonald-Hyman, Cameron, Loschi, Michael, Thangavelu, Govindarajan, Ma, Lie, Zaiken, Michael, Dysthe, Josh, Krepps, Walker, Panthera, Jamie, Hippen, Keli, Jameson, Stephen C., Miller, Jeffrey S., Cooper, Matthew A., Farady, Christopher J., Iwawaki, Takao, Ting, Jenny P.-Y., Serody, Jonathan S., Murphy, William J., Hill, Geoffrey R., Murray, Peter J., Bronte, Vincenzo, Munn, David H., Zeiser, Robert, and Blazar, Bruce R.
- Abstract
Myeloid-derived suppressor cells (MDSCs) can subdue inflammation. In mice with acute graft-versus-host disease (GVHD), donor MDSC infusion enhances survival that is only partial and transient because of MDSC inflammasome activation early posttransfer, resulting in differentiation and loss of suppressor function. Here we demonstrate that conditioning regimen–induced adenosine triphosphate (ATP) release is a primary driver of MDSC dysfunction through ATP receptor (P2x7R) engagement and NLR pyrin family domain 3 (NLRP3) inflammasome activation. P2x7R or NLRP3 knockout (KO) donor MDSCs provided significantly higher survival than wild-type (WT) MDSCs. Although in vivo pharmacologic targeting of NLRP3 or P2x7R promoted recipient survival, indicating in vivo biologic effects, no synergistic survival advantage was seen when combined with MDSCs. Because activated inflammasomes release mature interleukin-1β (IL-1β), we expected that IL-1β KO donor MDSCs would be superior in subverting GVHD, but such MDSCs proved inferior relative to WT. IL-1β release and IL-1 receptor expression was required for optimal MDSC function, and exogenous IL-1β added to suppression assays that included MDSCs increased suppressor potency. These data indicate that prolonged systemic NLRP3 inflammasome inhibition and decreased IL-1β could diminish survival in GVHD. However, loss of inflammasome activation and IL-1β release restricted to MDSCs rather than systemic inhibition allowed non-MDSC IL-1β signaling, improving survival. Extracellular ATP catalysis with peritransplant apyrase administered into the peritoneum, the ATP release site, synergized with WT MDSCs, as did regulatory T-cell infusion, which we showed reduced but did not eliminate MDSC inflammasome activation, as assessed with a novel inflammasome reporter strain. These findings will inform future clinical using MDSCs to decrease alloresponses in inflammatory environments.
- Published
- 2019
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31. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility.
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Xin, Liaobing, Lin, Xiaona, Pan, Yibin, Zheng, Xiaowen, Shi, Libing, Zhang, Yanling, Ma, Lie, Gao, Changyou, and Zhang, Songying
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MESENCHYMAL stem cells ,STROMAL cells ,COLLAGEN ,FERTILITY ,ESTROGEN receptors ,PROGESTERONE receptors - Abstract
In women of reproductive age, severe injuries to the endometrium are often accompanied by endometrial scar formation or intrauterine adhesions (IUAs), which can result in infertility or miscarriage. Although many approaches have been used to treat severe IUAs, high recurrence rates and endometrial thinning have limited therapeutic efficiency. In this study, a collagen scaffold (CS) loaded with human umbilical cord-derived mesenchymal stem cells (UC-MSCs) was fabricated and applied for endometrial regeneration. The CS/UC-MSCs promoted human endometrial stromal cell proliferation and inhibited apoptosis in vitro through paracrine effects. In a model of endometrial damage, transplantation with the CS/UC-MSCs maintained normal luminal structure, promoted endometrial regeneration and collagen remodeling, induced intrinsic endometrial cell proliferation and epithelium recovery, and enhanced the expression of estrogen receptor α and progesterone receptor. An improved ability of the regenerated endometrium to receive embryos was confirmed. Together, our results indicate that the CS/UC-MSCs promoted endometrial structural reconstruction and functional recovery. Topical administration of the CS/UC-MSCs after trans -cervical resection of adhesions might prevent re-adhesion, promote endometrium regeneration and improve pregnancy outcomes for patients with severe IUAs. Intrauterine adhesions due to severe endometrium injuries happen frequently in clinic and become one of the crucial reasons for women's infertility or miscarriage. Therefore, how to regenerate the damaged endometrium is a big challenge. In this study, a collagen scaffold (CS) loaded with human umbilical cord-derived mesenchymal stem cells (UC-MSCs) was fabricated and applied for endometrium regeneration. Herein, UC-MSCs, known for low immunogenicity and high proliferative potential, exhibit promising potential for endometrium regeneration; and collagen scaffolds provide suitable physical support. It was proved that transplantation with CS/UC-MSCs promoted endometrial regeneration and fertility restoration. It suggested that topical administration of CS/UC-MSCs in uterus could be a promising strategy for patients suffering severe intrauterine adhesion and infertility. [ABSTRACT FROM AUTHOR]
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- 2019
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32. Defined Substrate by Aptamer Modification with the Balanced Properties of Selective Capture and Stemness Maintenance of Mesenchymal Stem Cells.
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Wang, Xuemei, Zheng, Xiaowen, Duan, Yiyuan, Ma, Lie, and Gao, Changyou
- Published
- 2019
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33. Construction of Microreactors for Cascade Reaction and Their Potential Applications as Antibacterial Agents.
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Li, Tong, Li, Jiawei, Pang, Qian, Ma, Lie, Tong, Weijun, and Gao, Changyou
- Published
- 2019
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34. Bioassay-guided isolation of lanostane-type triterpenoids as α-glucosidase inhibitors from Ganoderma hainanense.
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Ma, Lie-Feng, Yan, Jia-Jie, Lang, Huang-Yan, Jin, Li-Chao, Qiu, Fei-Jun, Wang, Yue-Jun, Xi, Zhi-Fang, Shan, Wei-Guang, Zhan, Zha-Jun, and Ying, You-Min
- Abstract
Graphical abstract Highlights • α-Glucosidase inhibitory constituents of Ganoderma hainanense were investigated. • 22 Lanostane-type triterpenoids including 2 new compounds were identified. • 16 Compounds exhibited more potent α-glucosidase inhibitory activity than acarbose. • Preliminary structure-activity relationship was studied. Abstract Ganoderma hainanense , an edible mushroom mainly distributed in Southern China, has long been used as a functional food with considerable health benefits. Bioassay-guided phytochemical research on the fruiting bodies of G. hainanense led to the isolation of 22 lanostane-type triterpenoids, including two new compounds 3-acetyl-ganodermadiol (1) and 3-acetyl-lucidumol B (2). In an in-vitro assay, sixteen out of the twenty-two triterpenoids were found to possess more potent α-glucosidase inhibitory activity than the positive control acarbose. Preliminary structure-activity relationship (SAR) analysis indicated that the side chain structures of these compounds could significantly influence the activity. Results of the present study revealed that lanostane-type triterpenoids with α-glucosidase inhibitory activity may contribute to the hypoglycaemic effects of G. hainanense in the folk usage, and also the potentiality to develop G. hainanense as dietary supplement for glycemic control. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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35. Three new eudesmane sesquiterpenoids and a new dimer from the aerial part of Salvia plebeia R. Br.
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Ma, Lie-Feng, Xu, Hong, Wang, Ji-Dong, Tong, Xiang-Min, Zhan, Zha-Jun, Ying, You-Min, Wang, Jian-Wei, Zhang, Hui, and Shan, Wei-Guang
- Abstract
Four new compounds, including three eudesmane sesquiterpenoids ( 1 – 3 ) and a dimeric sesquiterpenoid lactone ( 4 ), were isolated from the aerial part of Salvia plebeia R. Br., together with two known eudesmanolides ( 5 – 6 ). Their structures were elucidated by extensive spectroscopic analysis including 1D and 2D NMR, HR-ESI–MS spectra. Antiproliferative activities of isolates against six human myeloid leukemia cell lines were evaluated, salplebeone D ( 1 ) and G ( 4 ) showed moderate inhibitory activity. In addition, compound 4 was a rare eudesmane sesquiterpenoid dimer obtained from this specie for the first time. [ABSTRACT FROM AUTHOR]
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- 2018
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36. Millisecond Response of Shape Memory Polymer Nanocomposite Aerogel Powered by Stretchable Graphene Framework
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Guo, Fan, Zheng, Xiaowen, Liang, Chunyuan, Jiang, Yanqiu, Xu, Zhen, Jiao, Zhongdong, Liu, Yingjun, Wang, Hong Tao, Sun, Haiyan, Ma, Lie, Gao, Weiwei, Greiner, Andreas, Agarwal, Seema, and Gao, Chao
- Abstract
Shape memory polymers (SMPs) change shapes as-designed through altering the chain segment movement by external stimuli, promising wide uses in actuators, sensors, drug delivery, and deployable devices. However, the recovery speed of SMPs is still far slower than the benchmark shape memory alloys (SMAs), originating from their intrinsic poor heat transport and retarded viscoelasticity of polymer chains. In this work, monolithic nanocomposite aerogels composed of bicontinuous graphene and SMP networks are designed to promote the recovery time of SMP composites to a record value of 50 ms, comparable to the SMA case. The integration of a stretchable graphene framework as a fast energy transformation grid with ultrathin polycaprolactone nanofilms (tunable at 2.5–60 nm) enables the rapid phase transition of SMPs under electrical stimulation. The graphene–SMP nanocomposite aerogels, with a density of ∼10 mg cm–3, exhibit a fast response (175 ± 40 mm s–1), large deformation (∼100%), and a wide response bandwidth (0.1–20 Hz). The ultrafast response of SMP nanocomposite aerogels confers extensive uses in sensitive fuses, micro-oscillators, artificial muscles, actuators, and soft robotics. The design of bicontinuous ultralight aerogels can be extended to fabricate multifunctional and multiresponsive hybrid materials and devices.
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- 2019
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37. Defined Substrate by Aptamer Modification with the Balanced Properties of Selective Capture and Stemness Maintenance of Mesenchymal Stem Cells
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Wang, Xuemei, Zheng, Xiaowen, Duan, Yiyuan, Ma, Lie, and Gao, Changyou
- Abstract
The recruitment of endogenous mesenchymal stem cells (MSCs), as an alluring approach for in situ tissue regeneration, always accompanies with other types of cells. Therefore, it is of enormous value to bestow a substrate with the property of selective capture to MSCs. However, it was reported that when MSCs are cultured on a substrate with excessive affinity, their stemness diminished. Therefore, constructing a substrate with the balanced ability of selective capture and stemness maintenance becomes a big challenge. In this study, an Aptamer 19S (Apt19S)-modified substrate was fabricated by grafting Apt19S on a PEGylated glass substrate. The X-ray photoelectron spectroscopy results verified that the antifouling poly(ethylene glycol) (PEG) layer was created. Tracking by ellipsometry, the thicknesses of PEG layers were proved to increase with PEG concentration. The results of the quartz crystal microbalance also validated that the Apt19S densities could be modulated by the concentrations of the Apt19S solution. The results of the cell adhesion assay indicated that the modification of Apt19S caused a significant increase in the adhesion ratio and area of rBMSCs. Selective adhesion was confirmed by coculture of rBMSCs with macrophages and NIH3T3 cells, demonstrating that a higher proportion of rBMSCs adhered to the Apt19S-modified substrate. The results of specific capture were further confirmed by a flow model to simulate the body fluid flow. The comprehensive results of reverse transcription polymerase chain reaction, immunofluorescence staining, proliferation capacity, and differentiation assay showed that the stemness of rBMSCs was maintained better on a substrate with the appropriate Apt19S density. All of these results indicated that Apt19S modification is an effective strategy to endow a substrate with the specific capture ability of MSCs, and the balance between selective capture and stemness maintenance can be achieved by the precise regulation of the aptamer density.
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- 2019
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38. Construction of Microreactors for Cascade Reaction and Their Potential Applications as Antibacterial Agents
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Li, Tong, Li, Jiawei, Pang, Qian, Ma, Lie, Tong, Weijun, and Gao, Changyou
- Abstract
Enzymatic cascade reactions in confined microenvironments play important roles in cellular chemical transformation. They also have important biotechnological and therapeutic applications. Here, enzymatic cascade microreactors (MRs) coupling glucose oxidase (GOx) and hemoglobin (Hb) (GOx-Hb MRs) were successfully fabricated by co-precipitation of GOx and Hb into a MnCO3template, followed by the assembly of a multilayer film on a template surface, slight cross-linking, and final removal of MnCO3. In the presence of glucose with blood-relevant concentration, the GOx-Hb MRs exhibited a higher cascade reaction activity under mild acidic conditions than that under neutral conditions at physiological temperature. The GOx-Hb MRs effectively consumed glucose to generate HO·at pH = 5, which significantly inhibited bacterial growth and biofilm formation. This kind of enzymatic cascade microreactors might be useful for applications in biomedical fields.
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- 2019
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39. Nuclear G protein-coupled oestrogen receptor (GPR30) predicts poor survival in patients with ovarian cancer
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Zhu, Cai-xia, Xiong, Wei, Wang, Ma-lie, Yang, Juan, Shi, Hui-juan, Chen, Han-qing, and Niu, Gang
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Objective To demonstrate the correlation between nuclear and cytoplasmic G protein-coupled oestrogen receptor (GPR30) expression and clinicopathological features and outcome in patients with ovarian cancer.Methods Nuclear and cytoplasmic GPR30 expressions were determined using immunohistochemistry to identify the intracellular location in tissues from patients with ovarian cancer. Data were correlated with clinicopathological characteristics and outcomes.Results Tissue samples were obtained from 110 patients with epithelial ovarian cancer between 2005 and 2010. Nuclear GPR30 was significantly more frequent in the group of patients with recurrence. The presence of nuclear GPR30 predicted lower overall survival) and 5-year progression-free survival in all patients with ovarian cancer and overall survival in patients with high grade ovarian cancer. Cytoplasmic GPR30 was observed significantly more often in advanced ovarian cancer and did not predict survival.Conclusion This study showed that nuclear GPR30 is an independent negative prognostic indicator in patients with ovarian cancer, especially in those with a high grade malignancy.
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- 2018
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40. In situassembly of fibrinogen/hyaluronic acid hydrogel via knob-hole interaction for 3D cellular engineering
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Huang, Shengjie, Wang, Chunfen, Xu, Jingwei, Ma, Lie, and Gao, Changyou
- Abstract
Hyaluronic acid (HA)-based hydrogels have applied widely for biomedical applications due to its biocompatibility and biodegradability. However, the use of initiators or crosslinkers during the hydrogel formation may cause cytotoxicity and thereby impair the biocompatibility. Inspired by the crosslinking mechanism of fibrin gel, a novel HA-based hydrogel was developed via the in situsupramolecular assembly based on knob-hole interactions between fibrinogen and knob-grafted HA (knob-g-HA) in this study. The knob-grafted HA was synthesized by coupling knob peptides (GPRPAAC, a mimic peptide of fibrin knob A) to HA via Michael addition. Then the translucent fibrinogen/knob-g-HA hydrogels were prepared by simply mixing the solutions of knob-g-HA and fibrinogen at the knob/hole ratio of 1.2. The rheological behaviors of the fibrinogen/knob-g-HA hydrogels with the fibrinogen concentrations of 50, 100 and 200 mg/mL were evaluated, and it was found that the dynamic storage moduli (G′) were higher than the loss moduli (G″) over the whole frequency range for all the groups. The SEM results showed that fibrinogen/knob-g-HA hydrogels presented the heterogeneous mesh-like structures which were different from the honeycomb-like structures of fibrinogen/MA-HA hydrogels. Correspondingly, a higher swelling ratio was obtained in the groups of fibrinogen/knob-g-HA hydrogel. Finally, the cytocompatibility of fibrinogen/knob-g-HA hydrogels was proved by live/dead stainings and MTT assays in the 293T cells encapsulation test. All these results highlight the biological potential of the fibrinogen/knob-g-HA hydrogels for 3D cellular engineering.
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- 2017
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41. A construct of adipose-derived mesenchymal stem cells—laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury
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Dai, Yangyang, Xin, Liaobing, Hu, Sentao, Xu, Shiqian, Huang, Dong, Jin, Xiaoying, Chen, Jianmin, Chan, Rachel Wah Shan, Ng, Ernest Hung Yu, Yeung, William Shu Biu, Ma, Lie, and Zhang, Songying
- Abstract
Severe endometrium damage causes pathological conditions such as thin endometrium and intrauterine adhesion, resulting in uterine factor infertility. Mesenchymal stem cell (MSC) therapy is a promising strategy in endometrial repair; yet, exogenous MSCs still raise concerns for safety and ethical issues. Human adipose-derived mesenchymal stem cells (ADMSCs) residing in adipose tissue have high translational potentials due to their autologous origin. To harness the high translation potentials of ADMSC in clinical endometrium regeneration, here we constructed an ADMSCs composited porous scaffold (CS/ADMSC) and evaluated its effectiveness on endometrial regeneration in a rat endometrium-injury model. We found that CS/ADMSC intrauterine implantation (i) promoted endometrial thickness and gland number, (ii) enhanced tissue angiogenesis, (iii) reduced fibrosis and (iv) restored fertility. We ascertained the pro-proliferation, pro-angiogenesis, immunomodulating and anti-fibrotic effects of CS/ADMSC in vitroand revealed that the CS/ADMSC influenced extracellular matrix composition and organization by a transcriptomic analysis. Our results demonstrated the effectiveness of CS/ADMSC for endometrial regeneration and provided solid proof for our future clinical study.
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- 2023
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42. Data-driven thermal preference prediction model with embodied air-conditioning sensors and historical usage behaviors.
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Luo, Maohui, Jiang, Kunyu, Wang, Jilong, Feng, Wei, Ma, Lie, Shi, Xudong, and Zhou, Xiang
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AIR conditioning ,PREDICTION models ,DETECTORS ,THERMAL comfort ,SMART homes - Published
- 2022
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43. Synthesis of poly(ester-carbonate) with a pendant acetylcholine analog for promoting neurite growth.
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Xing, Dongming, Ma, Lie, and Gao, Changyou
- Subjects
POLYESTERS ,POLYCARBONATES ,POLYMERIZATION ,ACETYLCHOLINE ,CELL growth ,BIODEGRADABLE products ,CELL adhesion ,NERVOUS system regeneration - Abstract
The modification of biodegradable polyesters with bioactive molecules has become an important strategy for controlling neuron adhesion and neurite outgrowth in nerve regeneration. In this study we report a biodegradable poly(ester-carbonate) with a pendant acetylcholine analog, which a neurotransmitter for the enhancement of neuron adhesion and outgrowth. The acetylcholine-functionalized poly(ester-carbonate) (Ach-P(LA-ClTMC)) was prepared by copolymerizing l -lactide (LA) and 5-methyl-5-chloroethoxycarbonyl trimethylene carbonate (ClTMC), followed by quaternization with trimethylamine. The acetylcholine analog content could be modulated by changing the molar feeding fraction of ClTMC. The incorporation of the acetylcholine analog improved the hydrophilicity of the films, but the acetylcholine analog content did not significantly influence the surface morphology of the acetylcholine-functionalized films. The results of PC12 cell culture showed that the acetylcholine analog promoted cell viability and neurite outgrowth in a concentration-dependent manner. The longest length of neurite and the percentage of cells bearing neurites were obtained on the Ach-P(LA-ClTMC)-10 film. All the results indicate that the integration of the acetylcholine analog at an appropriate fraction could be an effective strategy for optimizing the existing biodegradable polyesters for nerve regeneration applications. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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44. An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells
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Xin, Liaobing, Zheng, Xiaowen, Chen, Jianmin, Hu, Sentao, Luo, Yilun, Ge, Qunzi, Jin, Xiaoying, Ma, Lie, and Zhang, Songying
- Abstract
Severe intrauterine adhesions (IUAs), characterized by inadequate endometrial repair and fibrosis, can lead to infertility. Stem cell‐based therapies, which deliver mesenchymal stem cells (MSCs) to the wound site, hold a considerable promise for endometrium regeneration. However, some notable hurdles, such as stemness loss, immunogenicity, low retention and survival rate, limit their clinical application. Evidence shows a strategy of mobilizing endogenous MSCs recruitment can overcome the traditional limitations of exogenous stem cell‐based therapies. Here, an acellular biomaterial named stromal derived factor‐1 alpha (SDF‐1α)/E7‐modified collagen scaffold (CES) is explored. CES based on harnessing the innate regenerative potential of the body enables near‐complete endometrium regeneration and fertility restoration both in a rat endometrium acute damage model and a rat IUA model. Mechanistically, the CES implantation promotes endogenous MSCs recruitment via a macrophage‐coordinated strategy; then the homing MSCs exert the function of immunomodulation and altered local microenvironments toward regeneration. To conclude, CES, which can harness endogenous MSCs and overcome the traditional limitations of cell‐based therapies, can serve as a clinically feasible and cell‐free strategy with high therapeutic efficiency for IUA treatment. An acellular stromal derived factor‐1 alpha (SDF‐1α)/E7‐modified collagen scaffold (CES) is designed to treat intrauterine adhesions (IUAs) in rodent models. After CES implantation, endometrial repair and fertility restoration can be achieved. In mechanism, the CES implantation can promote endogenous MSCs’ recruitment via a macrophage‐coordinated strategy, and the homing MSCs can reciprocally exert immunomodulation on the phenotype of macrophages.
- Published
- 2022
- Full Text
- View/download PDF
45. Statistical model of the efficiency for spatial light coupling into a single-mode fiber in the presence of atmospheric turbulence
- Author
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Ma, Jing, Ma, Lie, Yang, Qingbo, and Ran, Qiwen
- Abstract
The average efficiency of spatial light coupling into a single-mode optical fiber is widely used but cannot estimate the signal-to-noise ratio (SNR) and bit error rate (BER) in free-space optical communication. We provide a statistical model for coupling efficiency and derive the exact expression of the probability density function (PDF). The simulation results confirm that the model is reasonable in the condition of different turbulence intensities and wavefront compensation terms, which is also consistent with our outdoor experiment. We also estimate the average SNR and BER using the PDF. The model is quite useful in a satellite-to-ground laser communication downlink.
- Published
- 2015
46. Fabrication and characterization of poly(l-lactide-co-glycolide) knitted mesh-reinforced collagen–chitosan hybrid scaffolds for dermal tissue engineering.
- Author
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Wang, Xingang, Li, Qiyin, Hu, Xinlei, Ma, Lie, You, Chuangang, Zheng, Yurong, Sun, Huafeng, Han, Chunmao, and Gao, Changyou
- Subjects
SKIN physiology ,TISSUE scaffolds ,TISSUE mechanics ,TISSUE engineering ,MICROFABRICATION ,COLLAGEN ,CHITOSAN ,BIOMEDICAL materials - Abstract
Abstract: Mechanical properties are essential considerations for the design of porous scaffolds in the field of tissue engineering. To develop a well-supported hybrid dermal substitute, poly(l-lactide-co-glycolide) (PLGA) yarns were knitted into a mesh with relative fixed loops, followed by incorporation into collagen–chitosan scaffolds (CCS) to obtain PLGA knitted mesh-reinforced CCS (PLGAm/CCS). The morphology and tensile strength in both the dry and wet state of PLGAm/CCS were investigated in vitro. To characterize the tissue response, specifically angiogenesis and tissue regeneration, PLGAm/CCS was embedded subcutaneously in Sprague–Dawley rats and compared with two control implants, i.e., PLGA mesh (PLGAm) and CCS. At weeks 1, 2, and 4 post surgery, tissue specimens were harvested for histology, immunohistochemistry, real-time quantitative PCR and Western blot analysis. These results demonstrated that the incorporation of PLGA knitted mesh into CCS can improve the mechanical strength with little influence on its mean pore size and porosity. After implantation, PLGAm/CCS can resist contraction and promote cell infiltration, neotissue formation, and blood vessel ingrowth, effectively. In conclusion, the mechanical strength of scaffolds can play a synergetic role in tissue regeneration and vascularization by maintaining its 3D microstructure. The ability of PLGAm/CCS to promote angiogenesis and induce in situ tissue formation demonstrates its strong potential in the field of skin tissue engineering. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
47. Enhanced angiogenesis of porous collagen scaffolds by incorporation of TMC/DNA complexes encoding vascular endothelial growth factor.
- Author
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Mao, Zhengwei, Shi, Haifei, Guo, Rui, Ma, Lie, Gao, Changyou, Han, Chunmao, and Shen, Jiacong
- Subjects
NEOVASCULARIZATION ,POROUS materials ,COLLAGEN ,TISSUE engineering ,REGENERATIVE medicine ,VASCULAR endothelial growth factors ,GENETIC code ,CHLORIDES - Abstract
Abstract: Angiogenesis of an implanted construct is one of the most important issues in tissue engineering and regenerative medicine, and can often take as long as several weeks. The vascular endothelial growth factor (VEGF) shows a positive effect on enhancing angiogenesis in vivo. But the incorporation of growth factors has many limitations, since they typically have half-lives only on the order of minutes. Therefore, in this work the DNA encoding VEGF was applied to enhance the angiogenesis of a collagen scaffold. A cationic gene delivery vector, N,N,N-trimethyl chitosan chloride (TMC), was used to form complexes with the plasmid DNA encoding VEGF. The complexes were then incorporated into the collagen scaffold, the loading being mediated by the feeding concentration and release in a sustained manner. In vitro cell culture demonstrated a significant improvement in the VEGF expression level from the TMC/DNA complexes containing scaffolds, in particular with a large amount of DNA. The scaffolds containing the TMC/DNA complexes were subcutaneously implanted into Sprague–Dawley mice to study their angiogenesis via macroscopic observation, hematoxylin–eosin staining and immunohistochemical staining. The results demonstrated that the incorporation of TMC/DNA complexes could effectively enhance the in vivo VEGF expression and thereby the angiogenesis of implanted scaffolds. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
48. The influence of polycaprolactone coating on the internalization and cytotoxicity of gold nanoparticles.
- Author
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Mao, Zhengwei, Wang, Bo, Ma, Lie, Gao, Changyou, and Shen, Jiacong
- Subjects
ELECTRON microscopy ,SOLUTION (Chemistry) ,ACETIC acid ,FATTY acids - Abstract
Abstract: The interaction between mesoscopic colloids and cells is largely dependent on the particle size and surface properties. Under a mild reaction condition, gold particles with an average diameter of ∼100 nm were prepared by incubating poly(dimethylsiloxane) film in HAuCl
4 /acetic acid solution. The particles were then transferred into a polycaprolactone (PCL) film by thermal pressing. Bare and PCL-coated particles were obtained by control over the extent of rinsing. The bare and PCL-coated gold particles were co-cultured with ECV-304 cells to examine the particle internalization and their influence on the cell morphology and cytotoxicity. Transmission electron microcopy observed the subcellular distribution of the gold particles, which were found in the cell compartments (endosomes or lysosomes), cytoplasm, nucleic envelope, and even nucleus regardless of the existence of PCL coating. However, scanning electron microscopy and β-tubulin staining revealed a significant change in terms of the cell morphology and cytoskeleton caused by the bare gold particles. Higher cytotoxicity was also determined for the bare gold particles. By contrast, no significant difference of the cell morphology and cytoskeleton change was caused by the PCL-coated gold particles, which have also shown lower cytotoxicity. [Copyright &y& Elsevier]- Published
- 2007
- Full Text
- View/download PDF
49. Design of gene-activated matrix for the repair of skin and cartilage
- Author
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Wang, Chunfen, Ma, Lie, and Gao, Changyou
- Abstract
Recently, tissue engineering has advanced markedly in the development of the regeneration of injured or diseased tissues and organs. Gene-activated matrix combines gene therapy and tissue engineering to create a promising solution with great potential for the restoration of the structure and function of damaged or dysfunctional tissues. The present review provides a comprehensive overview of the developments, applications and future prospects of gene-activated matrix as a substitute for tissue repair and regeneration. Our current research on skin and cartilage regeneration with gene-activated matrix is presented, and the key issues for future studies are also proposed.
- Published
- 2014
- Full Text
- View/download PDF
50. Poly(lactide-co-glycolide)/Fibrin Gel Construct as a 3D Model to Evaluate Gene Therapy of Cartilage in Vivo
- Author
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Li, Bo, Li, Feifei, Ma, Lie, Yang, Junzhou, Wang, Chunfen, Wang, Dongan, and Gao, Changyou
- Abstract
Combination of gene therapy with tissue engineering can enhance the interplay between cells and matrix, leading to better restoration and regeneration of tissues and organs in vivo. In this study the PLGA/fibrin gel hybrids were employed to load lipofectamine/pDNA-TGF-β1 complexes and mesenchymal stem cells (MSCs) (experimental group), acting as a cartilage-mimetic tissue platform. The gene complexes distributed more evenly in the hybrid scaffolds, whereas they adhered onto the pore walls of the PLGA sponges. The filled fibrin gel rendered gene release in a slower manner, too. Moreover, the fibrin gel entrapped MSCs and contributed to a higher cell loading density in the hybrid constructs. In vivo assay showed that in the defects implanted with the experimental constructs both gene and protein expression levels of TGF-β1 were significantly higher than those of the fibrin-free group at weeks 1, 3, and 6 after surgery. The full articular cartilage defects repaired by the experimental group for 12 w were resurfaced by neo-tissues with a similar thickness, cell arrangement, and color to the normal neighboring cartilage and abundant glycosaminoglycans.
- Published
- 2014
- Full Text
- View/download PDF
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