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Women with polycystic ovary syndrome (PCOS) are characterized by endocrine disorders accompanied by a decline in oocyte quality. In this study, we generated a PCOS mice model by hypodermic injection of dehydroepiandrosterone, and metformin was used as a positive control drug to study the effect of pachymic acid (PA) on endocrine and oocyte quality in PCOS mice. Compared with the model group, the mice treated with PA showed the following changes (slower weight gain, improved abnormal metabolism; increased development potential of GV oocytes, reduced number of abnormal MII oocytes, and damaged embryos; lower expression of ovarian-related genes in ovarian tissue and pro-inflammatory cytokines in adipose tissue). All these aspects show similar effects on metformin. Most notably, PA is superior to metformin in improving inflammation of adipose tissue and mitochondrial abnormalities. It is suggested that PA has the similar effect with metformin, which can improve the endocrine environment and oocyte quality of PCOS mice. These findings suggest that PA has the similar effect with metformin, which can improve the endocrine environment and oocyte quality of PCOS mice.

ABSTRACTSKAP2 (Src kinase-associated phosphoprotein 2), a substrate of Src family kinases, has been suggested to be involved in actin-mediated cellular processes. However, little is known about its role in mouse oocyte maturation. In this study, we thus investigated the expression, localization, and functions of SKAP2 during mouse oocyte asymmetric division. SKAP2 protein expression was detected at all developmental stages in mouse oocytes. Immunofluorescent staining showed that SKAP2 was mainly distributed at the cortex of the oocytes during maturation. Treatment with cytochalasin B in oocytes confirmed that SKAP2 was co-localized with actin. Depletion of SKAP2 by injection with specific short interfering RNA caused failure of spindle migration, polar body extrusion, and cytokinesis defects. Meanwhile, the staining of actin filaments at the oocyte membrane and in the cytoplasm was significantly reduced after these treatments. SKAP2 depletion also disrupted actin cap and cortical granule-free domain formation, and arrested a large proportion of oocytes at the telophase stage. Moreover, Arp2/3 complex and WAVE2 expression was decreased after the depletion of SKAP2 activity. Our results indicate that SKAP2 regulates the Arp2/3 complex and is essential for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

Multi-axis machining of a complex surface is not only efficient but also reduces workpiece set-up errors. However, engineers usually rely on experience when determining set-up orientation and often cannot finish complex surfaces in one set-up. In this paper, a rational evaluation method for workpiece orientation and configuration of machine tool before tool path planning is proposed to increase the efficiency and reliability of process planning.By incorporating the visibility cone into workspace analysis, a software module is constructed in this research for optimum workpiece orientation and configuration of machine tool. First, the configurations of three-, four- and five-axis machine tools were synthesised, and the workspace was then analysed using form-shaping function. Both position vector and normal vector of each point along the u and v directions in the machining surface were employed to calculate the visibility range as the point of the visibility cone. In 3-axis machining, all normal vectors of the surface were projected on a unit sphere. All normal vectors were ensured to be within the visibility range of the ball-mill cutter as the workpiece set-up orientation changed. In 4-axis machining, the intersection between the visibility cone of each point and the workspace was obtained by changing the set-up orientation. In 5-axis machining, according to different workspaces of 5-axis machine tools, the visibility range of the machining surface was determined to yield the intersection between the visibility cone and the workspace by changing the workpiece orientation.The proposed method for evaluating manufacturability has been demonstrated to generate optimum workpiece set-up orientation and the suitable configuration of machine tools. Tool interference with workpiece surface can be avoided, which can help improve the planning of tool path.