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Since the declaration of COVID-19 as a pandemic in early 2020, multiple variants of the severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) have been detected. The emergence of multiple variants has raised concerns due to their impact on public health. Therefore, it is crucial to distinguish between different viral variants. Here, we developed a machine learning web-based application for SARS-CoV-2 variant identification via duplex real-time polymerase chain reaction (PCR) coupled with high-resolution melt (qPCR-HRM) analysis. As a proof-of-concept, we investigated the platform’s ability to identify the Alpha, Delta, and wild-type strains using two sets of primers. The duplex qPCR-HRM could identify the two variants reliably in as low as 100 copies/μL. Finally, the platform was validated with 167 nasopharyngeal swab samples, which gave a sensitivity of 95.2%. This work demonstrates the potential for use as automated, cost-effective, and large-scale viral variant surveillance.

Gallium oxyhydroxide (GaOOH) is a wide band gap semiconductor of interest for a variety of applications in electronics and catalysis where the synthesis of the crystalline form is usually achieved via hydrothermal routes. Here we synthesize GaOOH via the electrochemical oxidation of gallium based liquid metals in solutions of 0.1 M NaNO3electrolyte with pH adjusted over the range of 7–8.4 with NaOH. This electrochemical approach employed under ambient conditions results in the formation of crystalline oblong shaped α-GaOOH nanoparticles from both liquid gallium and liquid galinstan which is a eutectic based on Ga, In, and Sn. The size and shape of the GaOOH particles could be controlled by the solution pH. The product is characterized with scanning electron microscopy, transmission electron microscopy, X-ray diffraction, UV–visible spectroscopy, and photoluminescence spectroscopy. During the electrochemical oxidation process, the liquid metal drop was found to expand significantly in the case of galinstan due to a constant electrowetting effect which resulted in the continuous expulsion of nanomaterial from the expanding liquid metal droplet. This electrochemical approach may be applicable to other liquid metals for the fabrication of metal oxide nanomaterials and also demonstrates that significant chemical reactions may be occurring at the surface of liquid metals that are actuated under an applied electric field in aqueous electrolytes.