1. Immune correlates of the differing pathological and therapeutic effects of neoadjuvant chemotherapy in breast cancer.
- Author
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Kim, Ryungsa, Kawai, Ami, Wakisaka, Megumi, Sawada, Sayaka, Shimoyama, Mika, Yasuda, Naomi, Hidaka, Masayuki, Morita, Yukitaka, Ohtani, Shoichiro, and Arihiro, Koji
- Subjects
TREATMENT effectiveness ,AXILLARY lymph node dissection ,IMMUNOSUPPRESSION ,CANCER chemotherapy ,VASCULAR endothelial growth factors ,KILLER cells - Abstract
To evaluate immune responses paralleling the pathological and therapeutic effects of neoadjuvant chemotherapy (NAC) in the tumor microenvironment of breast cancer. 38 patients with stages II and III breast cancer received NAC followed by surgery in 2012–2018. Peripheral natural killer (pNK) cell activity, tumor-infiltrating lymphocytes (TILs), and levels of tumor microenvironmental factors were assessed before and after NAC. In univariate analysis, grade 2 (G2) and better therapeutic effects were significantly associated with high post-NAC levels of NK cells and interleukin-6, and tended to be associated with higher CD4, CD8 and CTLA-4 transcripts. Disappearance of axillary lymph node metastasis (Ax+) was significantly associated with 1) increased NK and pNK levels, 2) decreased vascular endothelial growth factor (VEGF) transcripts after NAC, 3) the presence of ≥5% TILs, and tended to be associated with higher CTLA-4 levels before NAC. Multivariate analysis showed that G2 and better therapeutic effects were significantly associated with higher NK levels after NAC (OR = 1.07, 95% CI 1.00–1.14; p = 0.0255), and that disappearance of Ax+ was significantly associated with the presence of ≥5% pre-NAC TILs (OR = 19.87, 95% CI 2.24–175.80; p = 0.0072). Increased NK cells after NAC, together with increased CD4
+ and CD8+ T-cells, and decreased CTLA-4+ T cells and VEGF correlate with beneficial therapeutic effects. Systemic activation of pNK cell activity and the presence of pre-NAC TILs may improve the elimination of Ax + together with decreased immunosuppression by VEGF in tumors. [ABSTRACT FROM AUTHOR]- Published
- 2020
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