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ß-Mannanase produced by Bacillus sp. W-2, isolated from decayed commercial konjak cake, was purified from the culture supernatant by (NH4)2 SO4 precipitation, adsorption to konjak gel, and column chromatography with DEAE-cellulose, Sephadex G-100 and Sephacryl S-200. Its molecular size was estimated by SDS-PAGE as 40 kDa, and by gel filtration as 36 kDa. The enzyme was most active at pH 7 and 70°C and was stable for at least 1 h between pH 5 and 10 and below 60°C. Its activity was completely inhibited by Hg2+. The enzyme hydrolysed galactomannan better than glucomannan and mainly produced mannose and mannobiose.

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IT may perhaps interest you and your readers to hear that by the Imperial ordinance of June 25 a committee has been established for the investigation of the earthquake phenomena, with the view of finding methods of predicting earthquakes, if possible, and of ascertaining the nature of construction, building, and otherwise, best calculated to resist the effect of the shocks. President Kato, of the Imperial University, has been nominated the president, and myself the secretary. Other members of the Committee are Furnichi (Director and Professor of Civil Engineering, Engineering College, and Head of the Engineering Bureau of the Department of the Interior), Tatonno (Professor of Architecture, Engineering College), Tanabe (Professor of Civil Engineering, Engineering College), Tanakadate (F.R. S. E., and Professor of Physics, Science College), and Nagaoka (Assistant Professor of Physics, Science College), Koto (Professor of Geology, Science College), and Kochibe (of the Geological Survey), Sekiya and Omori (Seismologists), Nakamura (of the Meteorological Bureau), and a foreign member, Prof. J. Milne Other members will be nominated by and by. The Parliament has granted 42,000 yen for this year, chiefly for the purchase of various instruments. The committee will be glad to receive any communication or suggestion on the subject Address: Earthquake Investigation Committee, care of the Department of Education, Japan.

THE following communication may perhaps interest your readers.