1. Identification of Cardiomyocyte-Fated Progenitors from Human-Induced Pluripotent Stem Cells Marked with CD82
- Author
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Takeda, Masafumi, Kanki, Yasuharu, Masumoto, Hidetoshi, Funakoshi, Shunsuke, Hatani, Takeshi, Fukushima, Hiroyuki, Izumi-Taguchi, Akashi, Matsui, Yusuke, Shimamura, Teppei, Yoshida, Yoshinori, and Yamashita, Jun K.
- Abstract
Here, we find that human-induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM)-fated progenitors (CFPs) that express a tetraspanin family glycoprotein, CD82, almost exclusively differentiate into CMs both in vitroand in vivo. CD82 is transiently expressed in late-stage mesoderm cells during hiPSC differentiation. Purified CD82+cells gave rise to CMs under nonspecific in vitroculture conditions with serum, as well as in vivoafter transplantation to the subrenal space or injured hearts in mice, indicating that CD82 successfully marks CFPs. CD82 overexpression in mesoderm cells as well as in undifferentiated hiPSCs increased the secretion of exosomes containing β-catenin and reduced nuclear β-catenin protein, suggesting that CD82 is involved in fated restriction to CMs through Wnt signaling inhibition. This study may contribute to the understanding of CM differentiation mechanisms and to cardiac regeneration strategies.
- Published
- 2018
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