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An assist flow estimation scheme for a moving-actuator biventricular assist device (MA-BVAD) using interventricular pressure (IVP) has been developed. The scheme uses a waveform feature parameter of IVP, peak IVP time (PIT), for estimation of the filling volumes of both left and right blood sacs simultaneously. In a regression analysis on data from an in vivo test in an 85 Kg male calf for 20 days, the PIT was found to have high correlation with the blood sac filling volume (R=0.883: left filling volume, R=0.967: right filling volume). A conceptual equation hypothesizing this correlation between PIT and filling volume was established based on the observation and the unknown parameters were identified using least squares parameter optimization. The estimation equation identified proved highly accurate (R=0.916 for left flow, R=0.970 for right flow). The accuracy of the estimation scheme promises very good practical applicability.

Background A precondition for the successful formation of tissue-engineered heart valves is the generation of a proper matrix on biodegradable scaffolds over a limited period of time. The aim of this study was to find an effective method of seeding autologous cells on these scaffolds to create a new matrix for heart valves.Methods Myofibroblasts and endothelial cells were isolated and cultured from an ovine artery. A synthetic biodegradable scaffold consisting of polyglycolic and polylactic acids was seeded first with the myofibroblasts, then coated with endothelial cells. Three different methods of myofibroblast seeding were compared: I) daily seeding of myofibroblasts (1×106) for ten days and culture for four days; II) seeding of myofibroblasts (1×107) and culture for 14 days with the use of a simple medium; III) seeding of myofibroblasts (1×107) with the use of a medium containing collagen and culture for 14 days. Light and electron microscopic analyses were perfomed.Results The group that used the medium containing collagen showed the best results in terms of seeding efficiency.Conclusion Seeding autologous cells with a medium containing collagen onto the scaffold showed the largest cell population and might generate the best matrix on the scaffold.

Aortic pressure (AoP) estimation is a very important study for the artificial heart. In this paper, we developed a AoP estimation model for the moving-actuator type total artificial heart (MHTAH) that was being developed at Seoul National University Hospital. The proposed model is simple and provides beat-by-beat mean AoP estimation. Moreover, it uses non-invasively acquired signals. Model parameters were adjusted with in vitro data by least mean square (LMS) algorithm. Results showed that the proposed scheme gives a mean estimation error of about 8 (mmHg). This ensures the suitability of the proposed model. The proposed approach can also be applied to the model setup of pulmonary arterial pressure (PAP) estimation by using the symmetric characteristics between AoP and PAP.

Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H"2O"2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage.

Managing subchondral bone defects in proximal tibia fractures after plateau reduction is an important consideration. ChronOS Inject is a recently developed calcium phosphate bone substitute that shows relatively fast osteointegration.