Nurjayadi, Muktiningsih, Pertiwi, Y.P., Islami, N., Azizah, N., Efrianti, U.R., Saamia, V., Wiranatha, I.M., Nastassya, L., and El-Enshasye, H. Ali
Salmonella typhi is one of the bacteria that cause food poisoning. Food poisoning and food safety become important studies along with increasing awareness and attention around the world. Various methods for detecting food poisoning bacteria are still being developed. The aim of the research is to develop detection kits of food poisoning bacteria with high sensitivity, specificity, accuracy and fast detection through RT-PCR method. The results showed that the primer pair could amplify fim-C of S. typhi gene DNA fragments as template as much 56 ng with Ct 14.783 and 14.923. The results showed that the primer pair could amplify fim-C of S. typhi gene DNA fragments at template concentration of 4.528 pg/μL with Ct 23.9. The specificity test showed that the primer can distinguish target and non-target bacteria, namely Shigella with Ct 27,949 and E. coli with Ct 26,036. Concentration of S.typhi as much of 10−6 CFU/mL in eggs can still be detected by producing a value of Ct 15,736 and 15,895. Based on the results it can be concluded that the development of the detection of S. typhi in contaminated food was successfully carried out, so that this detection device can be used for forensic laboratories or food testing laboratories with a small sample number, and provide specific, sensitive and fast results. • Fim-C-S. typhi primer pairs can be used for the detection of S. typhi in contaminated egg. • S.typhi as much of 10−6 CFU/mL in eggs can still be detected by producing a value of Ct 15,736 and 15,895. • RT PCR can be used as detection food poisoning bacteria with high sensitivity, specificity, accuracy and fast detection. [ABSTRACT FROM AUTHOR]