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Intratumor heterogeneity (ITH) represents a major challenge for anticancer therapies. An integrated, multidimensional, multiregional approach dissecting ITH of the clear cell renal cell carcinoma (ccRCC) tumor microenvironment (TME) is employed at the single-cell level with mass cytometry (CyTOF), multiplex immunofluorescence (MxIF), and single-nucleus RNA sequencing (snRNA-seq) and at the bulk level with whole-exome sequencing (WES), RNA-seq, and methylation profiling. Multiregional analyses reveal unexpected conservation of immune composition within each individual patient, with profound differences among patients, presenting patient-specific tumor immune microenvironment signatures despite underlying genetic heterogeneity from clonal evolution. Spatial proteogenomic TME analysis using MxIF identifies 14 distinct cellular neighborhoods and, conversely, demonstrated architectural heterogeneity among different tumor regions. Tumor-expressed cytokines are identified as key determinants of the TME and correlate with clinical outcome. Overall, this work signifies that spatial ITH occurs in ccRCC, which may drive clinical heterogeneity and warrants further interrogation to improve patient outcomes.

This paper discusses security problems of developing municipal management information systems for Department of Municipal Procurement of the Krasnoyarsk City Administration (Russia). Authors describe the problem, goals, and tasks to be solved. Some security aspects are given, as well as original extended role-based security access control model for web applications and web services. This work describes two information systems developed by specialists of the Institute of Computational Modelling of the Siberian Branch of the Russian Academy of Sciences using described approaches. The systems have been in production for several years. The methods used to protect information are given, the structure of software is presented.

This article represents the method of creation and verification of a simulation model consisting of graphical structure duplicating the elements of spacecraft onboard equipment and the knowledge base describing the methods of its function. The authors have developed software tools for model building and verification with the help of visual components converting formal description of the model and the knowledge base into interactive graphical images of infographics. The method can be used at any stage of onboard equipment designing, however it is most efficient at the initial stages.

This article represents the method of creation and verification of a simulation model consisting of graphical structure duplicating the elements of spacecraft onboard equipment and the knowledge base describing the methods of its function. The authors have developed software tools for model building and verification with the help of visual components converting formal description of the model and the knowledge base into interactive graphical images of infographics. The method can be used at any stage of onboard equipment designing, however it is most efficient at the initial stages.

This paper discusses security problems of developing municipal management information systems for Department of Municipal Procurement of the Krasnoyarsk City Administration (Russia). Authors describe the problem, goals, and tasks to be solved. Some security aspects are given, as well as original extended role-based security access control model for web applications and web services. This work describes two information systems developed by specialists of the Institute of Computational Modelling of the Siberian Branch of the Russian Academy of Sciences using described approaches. The systems have been in production for several years. The methods used to protect information are given, the structure of software is presented.