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The concentration of Cu in milkfish organs, muscle and liver as well as in water ponds located in Ujung Pangkah, Gresik in April 2015 were investigated. The milkfish in three ponds with different size which were 26 cm (small size), 32 cm (medium size) and 46 cm (large fish) were taken. The liver of mikfish with size 26 cm, 32 cm and 46 cm contained the highest concentration of Cu. Moreover, Bioconcentration Factor of Cu in liver small milkfish (26 cm) was 2 983 which is approximately 100 times higher compare to Biocencentration Factor of Cu in muscle of similar size of fish.