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Sealing of exposed dentinal tubules is generally considered the most effective strategy to treat dentin hypersensitivity. On this account, we fabricated a DP-bioglass paste that created a homogeneous blockage on open dentinal tubules and formed a deep precipitate within dentinal tubules. DP-bioglass paste was prepared by mixing 20% to 60% phosphoric acid and DP-bioglass to treat dentin surfaces. CO2 laser irradiation was used to melt the DP-bioglass paste. The results demonstrated that 30% phosphoric acid was the optimum concentration to produce homogeneous occlusion on exposed dentinal tubules and 60 μm of sealing depth. CO2 laser irradiation could melt the DP-bioglass paste and create about 10 μm of sealing depth. Moreover, temperature rise during CO2 laser irradiation was only 4.86±0.47°C. The results presented in this work suggested that DP-bioglass paste could produce considerable sealing depth in dentinal tubules with the potential of prolonging the therapeutic effect efficaciously. [ABSTRACT FROM AUTHOR]