1. Enhanced resistance to Botrytis cinereaand Rhizoctonia solaniin transgenic broccoli with a Trichoderma virideendochitinase gene
- Author
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YU, Ya, ZHANG, Lei, LIAN, Wei-ran, XU, Feng-feng, LI, Shuang-tao, XIANG, Juan, ZHANG, Guo-zhen, HU, Zan-min, ZHAO, Bing, REN, Shu-xin, and GUO, Yang-dong
- Abstract
A endochitinase gene (Tch) from the fungus Trichoderma viridewas introduced into broccoli (Brassica oleraceavar. italica) by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained and the presence of the Tchgene was confirmed by both PCR and Southern blot analysis. RT-PCR analysis showed an accumulation of the transcript encoding the endochitinase protein in the transgenic plants. Using real-time quantitative PCR, the expression profiling of endochitinase gene was analyzed. Primary transformants and selfed progeny were examined for expression of the endochitinase using a fluorometric assay and for their resistance to the pathogenic fungi Botrytis cinereaand Rhizoctonia solani. The endochitinase activities in T0in vitroplants, T0mature plants and T1mature plants were correlated with leaf lesions, and the transgenic line T618 had high endochitinse activities of 102.68, 114.53 and 120.27 nmol L−1MU min−1mg−1protein in the three kinds of plants, respectively. The endochitinase activity showed a positive correlation with the resistance to the pathogens. Most transgenic T0broccoli had increased resistance to the pathogens of B. cinereaand R. solaniin leaf assays and this resistance was confirmed to be inheritable. These findings suggested that expression of the Tchgene from T. viridecould enhance resistance to pathogenic fungi in Brassicaspecies.
- Published
- 2015
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