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Internal combustion engine (ICE) vehicles are an important source of pollution gases; therefore, several research groups are implementing systems to reduce hydrocarbon emissions. Gasoline enrichment with alternative fuels such as ethanol, hydrogen, and others has been considered. This paper investigates the effect of a hydrogen–oxygen mixture (oxyhydrogen gas) injected to a gasoline ICE. The test module was adapted to a modified ICE with a hydrogen and oxygen intake system which is controlled by an electronic unit. Oxyhydrogen gas (OH2G) was produced by two water alkaline electrolyzers (WAEs) in a combined parallel-series arrangement, and their energy consumption ranged from 60 to 500 W with efficiency values between 50 and 65%. The WAEs used in this study were designed and built by our research group. The engine was working at a typical city-driving speed range from 1000 to 2000 rpm. The experimental work consisted of two main stages: In the first, the engine was tested only with gasoline, and in the second one, an oxyhydrogen gas volume was injected, and the total OH2G real intake in air flow was 903 smL min–1. Finally, the oxyhydrogen gas mixture allowed the reduction of gasoline consumption and contributed to a decrease in CO2emissions.