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Two phylogenetically distinct Vibrionaceae strains C4II189 T and C4V358 T isolated from reef seawater off Ishigaki Island, Japan, in 2014 were studied with advanced genome-based taxonomy approaches. All aspects of phylogenetic (16S rRNA phylogeny, MLSA), phenotypic and genetic (ANI, DDH, AAI, and the number of core genes) cohesions between the two identified species were high enough to propose them as members of a new genus within the family Vibrionaceae . Consequently, an eighth genus Thaumasiovibrio gen. nov. is proposed that contains two new species Thaumasiovibrio occultus sp. nov. strain C4II189 T (=DSM 101554 T = JCM 31629 T ) (type species) and Thaumasiovibrio subtropicus sp. nov. strain C4V358 T (=DSM 101555 T = JCM 31630 T ). Thaumasiovibrio species were phylogenetically distinct from the other Vibrionaceae species based on pyrH gene sequences. The combination of catalase negative, sensitivity to vibriostatic agent O/129, and green colony formation on TCBS for the phylogenetically affiliated strains was the diagnostic features for the current tentative identification of this genus. [ABSTRACT FROM AUTHOR]

Five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off Okinawa in coral reef areas. These strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (MLSA) and in silico DNA–DNA similarity ( in silico DDH). Phylogenetic analyses on the basis of 16S rRNA gene sequences revealed that the isolates could be assigned to the genus Vibrio , however they were not allocated into any distinct cluster with known Vibrionaceae species. MLSA based on eight protein-coding genes ( gapA , gyrB , ftsZ , mreB , pyrH , recA , rpoA , and topA ) showed the vibrios formed an outskirt branch of Halioticoli clade. The experimental DNA–DNA hybridization data revealed that the five strains were in the range of being defined as conspecific but separate from nine Halioticoli clade species. The G + C contents of the Vibrio ishigakensis strains were 47.3–49.1 mol%. Both Amino Acid Identity and Average Nucleotide Identity of the strain C1 T against Vibrio ezurae HDS1-1 T , Vibrio gallicus HT2-1 T , Vibrio halioticoli IAM 14596 T , Vibrio neonatus HDD3-1 T and Vibrio superstes G3-29 T showed less than 95% similarity. The genome-based taxonomic approach by means of in silico DDH values also supports the V. ishigakensis strains being distinct from the other known Halioticoli clade species. Sixteen traits (growth temperature range, DNase and lipase production, indole production, and assimilation of 10 carbon compounds) distinguished these strains from Halioticoli clade species. The names V. ishigakensis sp. nov. is proposed for the species of Halioticoli clade, with C1 T as the type strain (JCM 19231 T = LMG 28703 T ). [ABSTRACT FROM AUTHOR]

This article discusses a study which examined the disease caused by Vibrio harveyi strain CAIM 179 isolated from the lesion of a juvenile Litopenaeus vannamei shrimp affected by bright-red syndrome (BRS). The study collected shrimps and analyzed their bacteriology by examining their bodies. It determined the minimum inhibitory concentration (MIC) for 29 bacterial isolates of the specimens. The study concluded that the strain is the cause of BRS and poses a threat to the shrimp farming industry in Mexico.

Two novel strains C4III282T and C4III291 were isolated from seawater collected a site off the Taketomi coral reef. Phylogenetic analysis based on the 16S rRNA sequences revealed that the two strains belong to the genus Vibrio. MLSA using eight protein-coding genes (ftsZ , gapA , gyrB , mreB , pyrH , recA , rpoA , and topA) showed that C4III282T and C4III291 are closely related to the members of the Ponticus clade, namely Vibrio panuliri JCM 19500T, Vibrio ponticus DSM 16217T, and " Vibrio rhodolitus " G98. ANI and in silico DDH values with members of the Ponticus clade were 77.6-78.7% and 22.2-23.1, respectively. The name Vibrio taketomensis sp. nov. is proposed with C4III282T (CAIM 1928T = DSM 106943T = JCM 33434T) as the type strain. [ABSTRACT FROM AUTHOR]