Search

This study evaluated the effects of homogenous demineralized dentin matrix (HDDM) slices and platelet-rich plasma (PRP) in surgical defects created in the parietal bones of alloxan-induced diabetic rabbits, treated with a guided bone regeneration technique. Biochemical, radiographic, and histological analyses were performed. Sixty adult New Zealand rabbits were divided into five groups of 12: normoglycaemic (control, C), diabetic (D), diabetic with a PTFE membrane (DM), diabetic with a PTFE membrane and HDDM slices (DM–HDDM), and diabetic with PTFE membrane and PRP (DM–PRP). The quantity and quality of bone mass was greatest in the DM–HDDM group (respective radiographic and histological analyses: at 15 days, 71.70 ± 16.50 and 50.80 ± 1.52; 30 days, 62.73 ± 16.51 and 54.20 ± 1.23; 60 days, 63.03 ± 11.04 and 59.91 ± 3.32; 90 days, 103.60 ± 24.86 and 78.99 ± 1.34), followed by the DM–PRP group (respective radiographic and histological analyses: at 15 days 23.00 ± 2.74 and 20.66 ± 7.45; 30 days 31.92 ± 6.06 and 25.31 ± 5.59; 60 days 25.29 ± 16.30 and 46.73 ± 2.07; 90 days 38.10 ± 14.04 and 53.38 ± 9.20). PRP greatly enhanced vascularization during the bone repair process. Abnormal calcium metabolism was statistically significant in the DM–PRP group ( P < 0.001) for all four time intervals studied, especially when compared to the DM–HDDM group. Alkaline phosphatase activity was significantly higher in the DM–HDDM group ( P < 0.001) in comparison to the C, D, and DM–PRP groups, confirming the findings of intense osteoblastic activity and increased bone mineralization. Thus, HDDM promoted superior bone architectural microstructure in bone defects in diabetic rabbits due to its effective osteoinductive and osteoconductive activity, whereas PRP stimulated angiogenesis and red bone marrow formation. [ABSTRACT FROM AUTHOR]

Oral mucositis is a reaction to chemoradiation therapy during cancer treatment. The aim of this study was to investigate the use of amniotic membrane as a biological dressing for oral mucositis lesions in rats. Sixty Wistar rats were divided into three groups ( n = 20): control, 5-fluoruracil (5-FU), 5-fluoruracil + amniotic membrane (5-FU + AM). Each group was subdivided ( n = 5) according to the time interval to sacrifice (3, 7, 14, and 21 days). Histology (haematoxylin–eosin staining) and immunocytochemistry (anti-rat antibodies CD4, CD8, VEGF, and PCNA) were evaluated. Immunocytochemistry results were analyzed using one-way ANOVA and Tukey tests. The amniotic membrane (5FU + AM) played an important role in cell proliferation (PCNA 3 days 27.08 ± 4.65, 7 days 27.90 ± 3.34) and especially in neovascularization (VEGF 3 days 23.00 ± 1.40, 7 days 26.00 ± 0.95) for all time intervals, when compared to 5-FU (PCNA 3 days 23.12 ± 1.61, 7 days 37.21 ± 1.20; VEGF 3 days 17.05 ± 1.51, 7 days 8.45 ± 1.35) and control (PCNA 3 days 29.99 ± 0.92, 7 days 16.33 ± 2.88; VEGF 3 days 13.65 ± 0.55, 7 days 15.70 ± 1.39). It was biocompatible, showing significant differences compared to the other groups in CD4 ( F = 40.72; P = 0.001) and CD8 ( F = 69.99, P = 0.001) staining together, only during the inflammation phase (7 days). Amniotic membrane presented biocompatibility and stimulated cell proliferation and neovascularization, functioning as a promising biological dressing. [ABSTRACT FROM AUTHOR]

Highlights: [•] Absorption of carbon dioxide in ionic liquids containing carboxylate anions. [•] New processes for capture and separation of gases. [•] Chemisorption of the gas depends on the presence of water. [•] The mechanism of reaction depends on the nature of the ions of the ionic liquid. [ABSTRACT FROM AUTHOR]

Abstract: We report the first direct experimental determination of the enthalpies of solution of carbon dioxide in ionic liquids 1-hexyl-3-methylimidazolium bis[trifluoromethylsulfonyl]amide ([C6mim][NTf2]) and 1-octyl-3-methylimidazolium bis[trifluoromethylsulfonyl]amide ([C8mim][NTf2]) at 313.15K and pressures up to 5MPa. The experiments are based on the detection of heat of mixing of gas with ionic liquid using a flow calorimetric technique. For both ionic liquids, gas solubilities were derived from experimental calorimetric data and were compared with solubility data obtained by a newly designed high-pressure solubility measurement apparatus. Results of enthalpies of solution were compared to enthalpies derived from Henry''s law constant, available in literature. The analysis of the data reported herein confirms the consistency between the direct determination of the heats of solution and their derivation from precise enough solubility data. Also it was proven that, within the experimental uncertainty, it is possible to derive reliable solubility data from the calorimetric experiments. [Copyright &y& Elsevier]