Search

Chez le sujet jeune, le diagnostic clinique in vivo de maladie d’Alzheimer (MA) et de démence frontotemporale (DFT) peut s’avérer difficile. La référence standard est, à ce jour, basée sur l’analyse anatomopathologique post-mortem. Il est ainsi crucial de disposer de techniques fiables permettant de réaliser le diagnostic différentiel entre ces deux pathologies.

Spherulites ® are multilamellar vesicles made up of surfactant bilayers. These vesicles would potentially be very useful for the encapsulation and protection of molecules; however, traditional formulations of these vesicles are poor at retaining small hydrophilic molecules (below 1000 g/mol). In this study, we present new systems of Spherulites called complex dispersions. These are prepared by dispersing Spherulites in an oil medium, and then emulsifying this oily dispersion of Spherulites within an aqueous solvent. These new systems provide an additional oil barrier between encapsulated molecules and an external aqueous phase. We have used polarized light optical microscopy, X-ray diffraction and freeze–fracture electron microscopy to study a complex dispersion of Spherulites at all stages of its preparation. We first studied the sheared lamellar phase, followed by the dispersion of the multilamellar vesicles in the oily medium and finally the emulsification of the oily dispersion within the aqueous solvent. We compared our results on lamellar phases with previous results obtained with Spherulites directly dispersible in an aqueous medium. Since the formulation of our lamellar phase included a large percentage of oil as a component, we studied the localization of the oil in the lamellar structure. We also studied the influence of osmotic pressure on complex dispersions, because complex dispersions possess a double structure similar to that of water-in-oil-in-water emulsions and multiple emulsions are known to be sensitive to osmotic pressure. In conclusion, complex dispersions proved to be new potential carriers exhibiting some unique physical properties.