63 results on '"Gebhard, M."'
Search Results
2. Glycogen synthase kinase 3 in chronic rhinosinusitis: two faces of a single enzyme in one disease.
- Author
-
Linke R, Pries R, Könnecke M, Bruchhage KL, Böscke R, Gebhard M, and Wollenberg B
- Published
- 2013
- Full Text
- View/download PDF
3. Bioreactive Barriers: A Comparison of Bioaugmentation and Biostimulation for Chlorinated Solvent Remediation.
- Author
-
Lendvay, J.M., Löffler, F.E., Dollhopf, M., Aiello, M.R., Daniels, G., fathepure, B.Z., Gebhard, M., Heine, R., Helton, R., Shi, J., Krajmalnik-Brown, R., Major Jr., C.L., Barcelona, M.J., Petrovskis, E., Hickey, R., Tiedje, J.M., and Adriaens, P.
- Published
- 2003
- Full Text
- View/download PDF
4. Analysis of 18F-FDG uptake pattern in PET for diagnosis of aseptic loosening versus prosthesis infection after total knee arthroplasty
- Author
-
Gravius, S., Gebhard, M., Ackermann, D., Büll, U., Hermanns-Sachweh, B., and Mumme, T.
- Published
- 2010
- Full Text
- View/download PDF
5. The QUEST Large Area CCD Camera
- Author
-
Baltay, C., Rabinowitz, D., Andrews, P., Bauer, A., Ellman, N., Emmet, W., Hudson, R., Hurteau, T., Jerke, J., Lauer, R., Silge, J., Szymkowiak, A., Adams, B., Gebhard, M., Musser, J., Doyle, M., Petrie, H., Smith, R., Thicksten, R., and Geary, J.
- Abstract
We have designed, constructed, and put into operation a very large area CCD camera that covers the field of view of the 1.2 m Samuel Oschin Schmidt Telescope at the Palomar Observatory. The camera consists of 112 CCDs arranged in a mosaic of four rows with 28 CCDs each. The CCDs are 600 × 2400 pixel Sarnoff thinned, back-illuminated devices with 13 ?m × 13 ?m pixels. The camera covers an area of 4.6° × 3.6° on the sky with an active area of 9.6 deg2. This camera has been installed at the prime focus of the telescope and commissioned, and scientific-quality observations on the Palomar-QUEST Variability Sky Survey were started in 2003 September. The design considerations, construction features, and performance parameters of this camera are described in this paper.
- Published
- 2007
- Full Text
- View/download PDF
6. Development and evaluation of a training module for the clinical introduction of the da Vinci robotic system in visceral and vascular surgery
- Author
-
Mehrabi, A., Yetimoglu, C., Nickkholgh, A., Kashfi, A., Kienle, P., Konstantinides, L., Ahmadi, M., Fonouni, H., Schemmer, P., Friess, H., Gebhard, M., Büchler, M., Schmidt, J., and Gutt, C.
- Abstract
With the increasing use of the surgical robotic system in the clinical arena, appropriate training programs and assessment systems need to be established for mastery of this new technology. The authors aimed to design and evaluate a clinic-like training program for the clinical introduction of the da Vinci robotic system in visceral and vascular surgery.Four trainees with different surgical levels of experience participated in this study using the da Vinci telemanipulator. Each participant started with an initial evaluation stage composed of standardized visceral and vascular operations (cholecystectomy, gastrotomy, anastomosis of the small intestine, and anastomosis of the aorta) in a porcine model. Then the participants went on to the training stage with the rat model, performing standardized visceral and vascular operations (gastrotomy, anastomosis of the large and small intestines, and anastomosis of the aorta) four times in four rats. The final evaluation stage was again identical to the initial stage. The operative times, the number of complications, and the performance quality of the participants were compared between the two evaluation stages to assess the impact of the training stage on the results.The operative times in the final evaluation stage were considerably shorter than in the initial evaluation stage and, except for cholecystectomies, all the differences reached statistical significance. Also, significantly fewer complications and improved quality for each operation in the final evaluation stage were documented, as compared with their counterparts in the initial evaluation stage. These improvements were recorded at each level of experience.The presented experimental small and large animal model is a standardized and reproducible training method for robotic surgery that allows evaluation of the surgical performance while shortening and optimizing the learning-curve.
- Published
- 2006
- Full Text
- View/download PDF
7. Angiogenesis Inhibition with TNP-470, 2-Methoxyestradiol, and Paclitaxel in Experimental Pancreatic Carcinoma
- Author
-
Ryschich, E., Werner, J., Gebhard, M. M., Klar, E., and Schmidt, J.
- Abstract
Inhibition of tumor angiogenesis is a novel therapeutic modality for various malignancies.
- Published
- 2003
8. In VivoAssessment of Angioarchitecture and Microcirculation in Experimental Liver Cancer: A New Model in Rats
- Author
-
Maksan, S.-M., Paulo, H., Ryschich, E., Kuntz, C., Gebhard, M., Klar, E., and Schmidt, J.
- Abstract
The rising incidence of unresectable hepatocellular malignancies remains a therapeutic challenge. Little is known about the mechanisms of angiogenesis and immunological aspects of liver tumor vessels. The aim of this study was to investigate hepatic and tumor microcirculation and leukocyte–endothelium interaction by means of intravital microscopy in a rat model of hepatocellular carcinoma. Off-line analysis showed that the angioarchitecture as well as blood flow velocity in liver cancer is heterogeneous. The leukocyte–endothelium interaction is significantly reduced compared to normal liver tissue. The data suggest that the main mechanism is a reduced expression of adhesion molecules demonstrating an effective immune escape mechanism of this tumor. The model represents a useful experimental tool to explore angiogenesis inhibition or immunological therapeutic strategies in experimental liver cancer.
- Published
- 2003
- Full Text
- View/download PDF
9. MR arthrography: pharmacology, efficacy and safety in clinical trials
- Author
-
Schulte-Altedorneburg, G., Gebhard, M., Wohlgemuth, W., Fischer, W., Zentner, J., Wegener, R., Balzer, T., and Bohndorf, K.
- Abstract
Abstract Objective. A meta-analysis was carried out of clinical trials published between 1987 and 2001 in respect of the clinical pharmacology and safety as well as the diagnostic efficacy of gadolinium-DTPA (Gd-DTPA) for direct intra-articular injection before MRI examination. Design. Scientific papers (clinical, postmortem and experimental studies) and information from the manufacturer regarding intra-articular injection of Gd-DTPA that addressed questions of mode of action, optimal concentration and dose, elimination and safety were reviewed. Clinical studies were classified according to their study design. The sensitivity, specificity and accuracy of MR arthrography (MRA) were compared with a "gold standard" (arthroscopy, arthrotomy) and other radiological evidence for different joints. Results. Fifty-two clinical studies of the overall 112 studies addressed aspects of diagnostic efficacy of MRA in patients or in healthy volunteers. The shoulder was the most assessed joint (29 of 52 studies). Good (>80%) or even excellent (90-100%) sensitivity, specificity and accuracy were found for MRA in most indications, especially for the shoulder and knee joints and induced extension of rotator cuff lesions, labrum abnormalities and postoperative meniscal tears. Two millimoles per liter has proven to be the best concentration for intra-articular administration of Gd-DTPA. After passive complete diffusion from the joint within 6-24 h, complete and rapid renal elimination takes place after intra-articular injection. Local safety proved to be excellent after intra-articular administration of Gd-DTPA. Regarding systemic tolerance almost no side effects have been reported, but the same safety considerations apply for intra-articular administration of Gd-DTPA as for intravenous injection. Conclusions. The diagnostic efficacy of intra-articular MRA in most clinical conditions affecting major joints is greater than that of plain MRI. In some diagnostic problems MRA achieves almost the same sensitivity and specificity as the surgical gold standard. Given a sterile application, the intra-articular administration of Gd-DTPA in a concentration of 2 mmol/l prior to MRI is a safe procedure.
- Published
- 2003
- Full Text
- View/download PDF
10. A Large-Area CCD Camera for the Schmidt Telescope at the Venezuelan National Astronomical Observatory
- Author
-
Baltay, C., Snyder, J. A., Andrews, P., Emmet, W., Schaefer, B., Sinnott, J., Bailyn, C., Coppi, P., Oemler, A., Sabbey, C. N., Sofia, S., van Altena, W., Vivas, A. K., Abad, C., Bongiovanni, A., Briceño, C., Bruzual, G., Della Prugna, F., Magris, G., Sánchez, Ge., Sánchez, Gu., Schenner, H., Stock, J., Adams, B., Gebhard, M., Honeycutt, R. K., Musser, J., Rengstorff, A., Ferrin, I., Fuenmayor, F., Hernandez, J., Naranjo, O., Rosenzweig, P., Harris, F., and Geary, J.
- Abstract
We have designed, constructed, and put into operation a large-area CCD camera that covers a large fraction of the image plane of the 1 m Schmidt telescope at Llano del Hato in Venezuela. The camera consists of 16 CCD devices arranged in a 4 × 4 mosaic covering 2.°3 × 3.°5 of sky. The CCDs are 2048 × 2048 LORAL devices with 15 ?m pixels. The camera is optimized for drift-scan photometry and objective-prism spectroscopy. The design considerations, construction features, and performance parameters are described in the following paper.
- Published
- 2002
- Full Text
- View/download PDF
11. Dopexamine attenuates microvascular perfusion injury of the small bowel in pigs induced by extracorporeal circulation.
- Author
-
Sack, F U, Reidenbach, B, Schledt, A, Dollner, R, Taylor, S, Gebhard, M M, and Hagl, S
- Abstract
Cardio-thoracic surgery with the use of extracorporeal circulation may lead to an impairment of splanchnic perfusion. The aim of this study was to investigate the effect of dopexamine on gastrointestinal microvascular perfusion failure due to extracorporeal circulation.
- Published
- 2002
- Full Text
- View/download PDF
12. Online Detection of Myocardial Ischemia by Near Infrared Spectroscopy with a Fiberoptic Catheter
- Author
-
Baykut, D., Gebhard, M. M., Bölükoglu, H., Kadipasaoglu, K., Hennes, S., Frazier, O. H., and Krian, A.
- Published
- 2001
- Full Text
- View/download PDF
13. Hirudin Protects from Leukocyte/Endothelial Cell Interaction Induced by Extracorporeal Circulation
- Author
-
Kamler, M., Chatterjee, T., Stemberger, A., Gebhard, M. M., Hagl, S., and Jakob, H.
- Published
- 2001
- Full Text
- View/download PDF
14. Intramucosal pH and liver endotoxin clearance during experimental liver transplantation
- Author
-
Golling, M., Bud, O., Frankenberg, M. v., Ulrich, F., Schäffer, F., Weiss, G., Mehrabi, A., Kraus, T., Urbaschek, R., Gebhard, M. M., Herfarth, C., and Klar, E.
- Abstract
Abstract The study was designed to assess the gastrointestinal ischaemia and the influence of the specific Kupffer cell toxin gadolinium chloride (GdCl3 ) on the hepatic and extrahepatic endotoxin [lipopolysaccharide (LPS)] clearance during experimental orthotopic liver transplantation (OLT) in pigs. In eight pig liver transplantations, the donors received 20 mg/kg of GdCl3 24 h before explantation, while controls (n = 8) received normal saline. Gastric and sigmoid intramucosal pH (pHi), LPS and endotoxin-neutralising capacity (ENC) levels were measured in the portal vein and superior vena cava after laparatomy, at the end of the anhepatic phase and 1 h after reperfusion. During the anhepatic phase, the sigmoid pHi decreased significantly from 7.32 ± 0.02 to 7.29 ± 0.03 (P < 0.001) and was associated with a substantial increase of portal LPS. Following reperfusion, the systemic LPS concentrations were significantly lower in the pretreated group [39 ± 23 pg/ml (Control); 14 ± 7 (GdCl3 );P < 0.05] suggesting an improved liver LPS clearance [86 % (GdCl3 ); 58.2 % (Control);P < 0.05]. This corresponded to an increased ENC in the pretreated group [118 ± 52 ENU/ml (GdCl3 ) vs 81 ± 45 ENU/ml (Control);P < 0.05]. The anhepatic phase induced splanchnic ischaemia which correlated with portal endotoxaemia. Donor preconditioning with GdCl3 leads to lower systemic LPS concentrations in the recipient and increases ENC values in the early phase after OLT. An improved hepatocellular LPS extraction and/or an activation of the extrahepatic reticulo-endothelial system as a result of GdCl3 treatment is discussed.- Published
- 2000
- Full Text
- View/download PDF
15. Dielectric Properties of Skeletal Muscle during Ischemia in the Frequency Range from 50 Hz to 200 MHz
- Author
-
SCHÄFER, M., KIRLUM, H.-J., SCHLEGEL, C., and GEBHARD, M. M.
- Abstract
The complex dielectric properties of canine skeletal muscles were measured at 25 °C during ischemia in the frequency range from 50 Hz to 200 MHz. The dielectric spectrum of skeletal muscle shows an ?-dispersion below 1 kHz and a ?-dispersion with a relaxation frequency of about 100 kHz. The ?-dispersion disappears between 450 and 500 min of ischemia time, the same time during which mechanical contraction was observed, and was restored later. During ischemia, the ?-dispersion is shifted continuously to higher frequencies; and at frequencies above 50 MHz, a decrease of the real part of the dielectric permittivity was measured. The dielectric loss factor decreases during ischemia at frequencies below 500 kHz, only interrupted by a short increase, coinciding with the disappearance of the ?-dispersion. The principal processes that happen during ischemia inside the skeletal muscle tissues were studied with the help of a model especially designed to simulate membrane effects on the dielectric spectrum. The disappearance of the ?-dispersion is explained by an increase of conductivity in the membrane of the sarcoplasmic reticulum. Shifting ?-dispersion to higher frequencies is a result of metabolically produced ions and therefore increasing conductivity of the intracellular medium. Decreasing dielectric permittivity at frequencies above 50 MHz and decreasing dielectric loss factor at low frequencies are caused by the cell edema.
- Published
- 1999
- Full Text
- View/download PDF
16. Gesellschafts- und zivilrechtliche Folgeprobleme der Sitztheorie
- Author
-
EIDENMÜLLER, HORST and REHM, GEBHARD M.
- Published
- 1997
- Full Text
- View/download PDF
17. Deep level transient spectroscopy on radioactive impurities: Demonstration for Si:111In*
- Author
-
Lang, M., Pensl, G., Gebhard, M., Achtziger, N., and Uhrmacher, M.
- Abstract
A defect specific analysis technique is introduced that combines the high concentration sensitivity of deep level transient spectroscopy (DLTS) with the radioactive transmutation of probe atoms; this technique results in the chemical identification of particular atoms which participate in the observed defect centres. The method is demonstrated for n- and p-type silicon samples, which are implanted with radioactive
111 In* ions. The activity of the indium ions decreases with half-life T1/2 of 2.83 d by a decay into stable cadmium ions. A series of Cd-related levels whose concentrations increase exponentially with the decay time of111 In* are identified in the DLTS spectra. Cd-diffused silicon samples are investigated for comparison; these samples reveal similar DLTS spectra. The analysis method described can be extended to all semiconductors and to a variety of radioactive probe atoms.- Published
- 1991
- Full Text
- View/download PDF
18. The ultrastructural effects of global ischaemia on Purkinje fibres compared with working myocardium: A qualitative and morphometric investigation on the canine heart
- Author
-
Schnabel, P. A., Richter, J., Schmiedl, A., Ramsauer, B., Bartels, U., Gebhard, M. M., Mall, G., and Bretschneider, H. J.
- Abstract
Summary During open heart surgery, reperfusion-induced arrhythmias arising after short periods of ischaemia may originate from subendocardial Purkinje fibres. We investigated the ultrastructure of these fibres during 30 min of global ischaemia at 25° C. The effects both with myocardial protection (HTK cardioplegia) and without it (pure ischaemia) were compared qualitatively and morphometrically. After 30 min pure ischaemia overcontraction of sarcomeres, hypercontraction and contraction bands, together with considerable changes in organelles, predominate over cellular oedema. In Purkinje fibres, both cellular and mitochondrial swelling were significantly increased within this 30-min time period from the onset of pure ischaemia. In contrast, following HTK cardioplegia and 30 min ischaemia, cellular and mitochondrial swelling remain moderate and over-contractions are almost entirely lacking. This means that despite remarkable differences between pure ischaemia and HTK cardioplegia in the degree of protection attained it is clear that, compared with the working myocardium, subendocardial Purkinje fibres do not display a higher resistance to early global ischaemia. Further investigations of this sensitivity of Purkinje fibres to global ischaemia and certain drugs may bring about new insights into myocardial protection and pharmacotherapy of arrhythmias.
- Published
- 1991
- Full Text
- View/download PDF
19. Ultrastructural effects induced by global ischaemia on the AV node compared with the working myocardium
- Author
-
Schnabel, Ph. A., Richter, J., Gebhard, M. M., Mall, G., Schmiedl, A., Clavien, H. -J., and Bretschneider, H. J.
- Abstract
Summary The cardiac conduction system is considered to be particularly resistant to ischaemia. Nevertheless, following open heart surgery with short periods of ischaemia disturbances in AV conduction or ventricular arrhythmia have been reported. We compared the ultrastructure of AV node and working myocardium following 30 min global ischaemia at 25° C, during pure ischaemia and with HTK cardioplegia qualitatively and morphometrically. After 30 min of pure ischaemia, interstitial and intracellular oedema together with considerable changes in organelles in AV nodes predominate over mainly cellular oedema in working myocardium. Sometimes irregular overcontractions of sarcomeres occur in the AV node, though very seldom in working myocardium. In pure ischaemia, mitochondrial swelling is comparable in both types of tissue. Following HTK cardioplegia and 30 min ischaemia, cellular oedema and mitochondrial swelling are significantly reduced in AV nodal cells and working myocardium, but remain more extensive in the AV nodes. Irregularities in the contractile state of sarcomeres are not observed. The extent of the ultrastructural alterations corresponds to the degree of metabolic change in the working myocardium. Thus, despite considerable differences during pure ischaemia and HTK cardioplegia, ultrastructurally the AV nodal cells do not display a greater resistance to ischaemia than working myocardium.
- Published
- 1990
- Full Text
- View/download PDF
20. The surface to volume ratio of mitochondria, a suitable parameter for evaluating mitochondrial swelling
- Author
-
Schmiedl, A., Schnabel, Ph. A., Mall, G., Gebhard, M. M., Hunneman, D. H., Richter, J., and Bretschneider, H. J.
- Abstract
Summary Cellular changes occuring in the left ventricular myocardium during ischaemia after different methods of cardiac arrest have been evaluated by morphological and morphometric parameters: volume densities of mitochondria (V
VMi ), sarcoplasm (VVSp ), myofibrils (VVMf ), surface densities of mitochondria (SVMi ). The surface to volume ratio of mitochondria (SV ratioMi ) has been used as an independent parameter of mitochondrial swelling.- Published
- 1990
- Full Text
- View/download PDF
21. Occurrence and prevention of contraction bands in Purkinje fibres, transitional cells and working myocardium during global ischaemia
- Author
-
Schnabel, Ph. A., Schmiedl, A., Ramsauer, B., Bartels, U., Gebhard, M. M., Richter, J., and Bretschneider, H. J.
- Abstract
Summary Contraction bands usually occur in the intramural working myocardium following post-ischaemic reperfusion. In the subendocardium, however, they are found during ischaemia. Thus, we ascertained the contraction states of Purkinje fibres, transitional cells, subendocardial and intramural parts of the working myocardium during 30 min global ischaemia at 25° C. The effects with and without myocardial protection were compared. At the onset of pure ischaemia contraction bands are completely lacking in all cell types. During pure ischaemia contraction bands are found in all subendocardial cell types but not in the intramural working myocardium. A peak of pathological contraction states is found in the intramural working myocardium at the onset (0 min), in the subendocardial working myocardium at 10 min, in the transitional cells and Purkinje fibres at 30 min of pure ischaemia. Histidine-, tryptophan-, ketoglutarate-enriched (HTK) cardioplegia prevents contraction bands completely at the onset of ischaemia and prevents both contraction bands and pathological contraction states during ischaemia almost completely. Striking differences in the physiological contraction states are seen only in the working myocardium: HTK cardioplegia brings about dominance of relaxation during ischaemia. These findings may be due mainly to the effects of global ischaemia on the one hand and to catecholamines, calcium and oxygen on the other.
- Published
- 1990
- Full Text
- View/download PDF
22. Design and fabrication of a thermal infrared emitter
- Author
-
Bauer, D., Heeger, M., Gebhard, M., and Benecke, W.
- Published
- 1996
- Full Text
- View/download PDF
23. Comparison of four alternative radiochemical purity testing methods for 99Tcm-sestamibi
- Author
-
HUNG, J. C., WILSON, M. E., GEBHARD, M. W., and GIBBONS, R. J.
- Abstract
Four different methods for determining the radiochemical purity (RCP) of 99Tcm-sestamibi have been proposed to replace the recommended thin-layer chromatography (TLC) method, as the recommended method is inconvenient and time-consuming. The purpose of this study was to compare the recommended TLC method with the four proposed rapid QC methods for 99Tcm-sestamibi: (1) chloroform extraction (CE), (2) mini-paper chromatography with chloroform/tetrahydrofuran (1:1, v/v) (MPC), (3) WatersTMSep-Pak®alumina N cartridge with 100 ethanol (SPE), and (4) WatersTMSep-Pak®C18 cartridge with normal saline (SPNS). For RCP values ranging from 21.8 to 98.7 (n= 20), both the CE and SPNS methods produced falsely high RCP values (RCP difference: 14.1 pm 23.5 for CE and 15.0 pm 24.9 for SPNS). The MPC and SPE methods were in good agreement (r= 0.98 for MPC and 0.88 for SPE) with the recommended TLC method over the critical RCP range (i.e. 77.0–98.7, n= 52) (RCP difference: −0.9 pm 1.2 for MPC and −4.3 pm 3.2 for SPE). None of the 99Tcm-sestamibi preparations that had been rejected (i.e. RCP <90) by the TLC method were accepted by either the MPC or SPE method. However, 4 of the 52 99Tcm-sestamibi preparations with acceptable RCP by the TLC method had unacceptable RCP by the MPC method, whereas 8 of the same 52 preparations were unacceptable by the SPE method. The SPE method requires the loading of 0.05–0.1 ml 99Tcm-sestamibi to the cartridge, which may consist of up to 1.2 GBq (33.3 mCi) 99Tcmactivity per application (based upon the 37 GBq or 1000 mCi/3 ml per kit preparation). The MPC method takes only a 5 μUl sample (9.3 MBq or 250 μUCi) and 3–4 min for each RCP analysis. In conclusion, the MPC method is the best alternative method for the RCP determination of 99Tcm-sestamibi, especially in emergency situations.
- Published
- 1995
24. Patterns of Structural Deterioration Due to Ischemia in Purkinje Fibres and Different Layers of the Working Myocardium
- Author
-
Schnabel, Ph. A., Richter, J., Schmiedl, A., Bach, F., Bartels, U., Ramsauer, B., Gebhard, M. M., and Bretschneider, H. J.
- Published
- 1991
- Full Text
- View/download PDF
25. Kinetics of In-H and Cd-H Complexes in Silicon
- Author
-
Gebhard, M., Vogt, B., and Witthuhn, W.
- Abstract
Not Available
- Published
- 1992
- Full Text
- View/download PDF
26. Messung der elektrischen Impedanz von Organen — Methodische Grundlagen - Measurement of Electrical Impedance in Organs – Fundamentals and Methodology
- Author
-
Gersing, E., Bach, F., Brockhoff, C., Gebhard, M. M., Kehrer, G., Meißner, A., and Bretschneider, H. J.
- Published
- 1991
- Full Text
- View/download PDF
27. Die Impedanzmessung zur Beurteilung von Ischämieschäden der humanen Leber in der Vorbereitung zur Transplantation
- Author
-
Erhard, J., Lange, R., Gersing, E., Scherer, R., Gebhard, M. M., Sanchez, P., Bretschneider, H. J., and Eigler, F. W.
- Abstract
In 22 human donor livers the measurement of the non-invasive bioelectrical impedance was performed prospectively to evaluate the degree of tissue damage sustained during cold ischemia. The results of the measurement were correlated with liver function, the method of organ preservation and the period of ischemia. The impedance was measured in vivo as 620 ohm (at 192 Hz), the phase angle as -7.4° (at 5 kHz). The results were compared with the data obtained from 72 patients who underwent elective laparotomies. The 22 donor livers were studied further during ischemia. The method was found to be a reliable way of detecting severe damage to the hepatocytes during the cold ischemia.
- Published
- 1993
- Full Text
- View/download PDF
28. Abstract of the 68th Meeting (Spring Meeting) 6–9 March 1990, Heidelberg
- Author
-
Sakmann, B., Schrader, J., Brenner, B., Murer, H., Boeckh, J., Handwerker, H. O., HonerjÄger, P., Dugas, M., Wang, G., DeLuca, A., Brinkmeier, H., Fakler, B., Pröbstle, T., Rüdel, R., Pohl, J. -A., Meves, H., Kroll, B., Bremer, S., Tümmler, B., Frömter, E., Schwegler, J. S., Steigner, W., Silbernagl, S., Pusch, Michael, Niemann, P., Schmidtmayer, J., Ulbricht, W., Hansen, G., Lönnendonker, U., Neumcke, B., Eickhorn, R., Hornung, D., Antoni, H., Penner, R., Neher, E., Takeshima, H., Nishimura, S., Numa, S., Melzer, W., Feldmeyer, D., Pohl, B., Zöllner, P., Müller, T. H., Swandulla, D., Misgeld, U, Ganitkevich, V. Ya., Isenberg, G., Cavalié, A., Allen, T. J. A., Trautwein, W., Pelzer, Siegried, Shuba, Yaroslav M., Asai, Tatsuya, Trautwein, Wolfgang, Brown, Arthur M., Birnbauner, Lutz, McDonald, Terence F., Pelzer, Dieter, Eckert, R., Hescheler, J., Rosenthal, W., Offermann, S., Krautwurst, D., Schultz, G., Kettenmahn, Helmut, Trotter, J., Verkhratsky, Alexe J N., Savtchenko, Alexej N., Verkhratsky, Alexej N., Schiefer, A., Klöckner, U., Partridge, L. D., SchÄfer, S., Jonas, P., Koh, D. S., Kampe, K., Hermsteiner, M., Vogel, W., Bauer, C. K., Schwarz, J. R., Fink, R. H. A., Wettwer, E., Weik, R., Schlatter, E., Bleich, M., Granitzer, M., Leal, T., Nagel, W., Crabbé, J., Lang, F., Kahn, E., Friedrich, F., Paulmichl, M., Hammerer, M., Maly, K., Grunicke, H., Böhm, T., Nilius, B., Gögelein, H., Dahlem, D., Weiss, H., Waldegger, S., Woell, E., Paulmichl, R., Ruppersberg, J. P., Schröter, K. H., Stocker, M., Pongs, O., Wittka, R., Boheim, G., Lichtinghagen, R, Augustine, C. K., Stühmer, W., Hoppe, Dorothe, Hoppe, D., Zittlau, K. E., Walther, C., Hatt, H., Franke, C., Quasthoff, S., Wischmeyer, E., Jockusch, H., Friedrich, M., Benndorf, K., Bollmann, G., Hirche, Hj., Hollunder-Reese, F., Mohrmann, M., Greger, R., Weber-Schürholz, S., Schürholz, T., Akabas, M., Landry, D., Al-Awqati, Q., Guse, A. H., Gercken, G., Meyerhof, W., Westphale, H. -J., Kerstins, U., Oberleithner, H., Tilmann, M., Kunzelmann, K., Klitsch, T., Siemen, D., Draguhn, A., Verdoorn, T. A., Pritchett, D. B., Seeburg, P. H., Malherbe, P., Möhler, H., Sakmann, B., Hatt, H., Dudel, J., Stern, P., Zufall, F., Rosenheimer, J., Smith, D. O., Dörner, R., Ballanyi, K., Schlue, W. -R., Kalthof, B., Pott, L., Busch, C., Konno, T., Stenql, M., Reinhardt, Ch., Kaiser, H., Baumann, R., Wilimzig, M., Eichenlaub, R., Neumann, E., Lessmann, V., Gottmann, K., Dietzel, I. D., Keller, B. U., Yaari, Y., Konnerth, A., Backus, K. H., Giller, T., Knoflach, F., Pflimlin, P., Trübe, G., von Blankenfeld, G., Ymer, S., Sontheimer, H., Ewert, M., Seeburg, P. H., Kettenmann, H., Schneggenburger, R., Paschke, D., Hülser, D. F., Ubl, J., Kolb, H. A., Ströttchen, J., Boheim, S., Wehner, F., Guth, D., Kinne, R. K. H., Hülser, D. F., Polder, H. R., Bödeker, D., Hoppe, Susanne, Höller, H., Hampe, W., Ruf, H., Schulz, I., Dehlinger-Kremer, M., Ozawa, T., Vasilets, L., Schmalzing, G., MÄdefessel, K., Biel, H., Schwarz, W., Burckhardt, B. C., Stallmach, N., MairbÄurl, H., Hoffman, J. F., Schömig, E., Heuner, A., Göbel, B. O., Siffert, W., Butke, A., Hoffmann, G., zu Brickwedde, M. -K. Meyer, Vetter, H., Düsing, R., Rosskopf, D., Osswald, U., Steffgen, J., Koepsell, H., Martens, H., Rübbelke, M., GÄbel, G., Arens, J., Stabel, J., Fischer, Y., Thomas, J., Rose, H., Kammermeier, H., Munsch, Thomas, Deitmer, Joachim W., Engelmann, B., Duhm, J., Deitmer, Joachim W., Gunzel, D., Galler, S., Fischer, H., Clauss, W., Van Driessche, W., Köckerling, A, Schulzke, JD, Sorgenfrei, D, Fromm, M, Simon, B., Ganapathy, V., Leibach, F. H., Burckhardt, G., Krattenmacher, R., Voigt, Rosita, Dietrich, S., Leyssens, A., Zhang, S. L., Weltens, R., Steels, P., Hoffmann, B., Heinz, M., Habura, B., Dörge, A., Rechkemmer, G., von Engelhardt, W., StrauB, O., Wiederholt, M., Margineanu, D. -G., Van Driessche, W., Kreusel, K. M., Fromm, M., Lempart, U., Sorgenfrei, D., Hegel, U., Augustin, A. J., . Goldstein, R., Purucker, E., Lutz, J., Illek, B., Thiele, K -P., Schwealer, JS., Dittmer, J., Bauer, C., Eckardt, K. -U., Dittmer, J., Neumann, R., Bauer, C., Kurtz, A., Fromm, H., Schulzke, J. D., Clausen, P., Krohn, A., Lüderitz, S., Hierholzer, K., Kersting, U., Woinowski, L., Gro\mann, R., Bin, X. U., Ellendorff, F., Nitschke, R., Fröbe, U., Scholz, H., della Bruna, R., Ehmke, H., Persson, P. B., Seyfarth, M., Kirchheim, H. R., Dietrich, M. S., Parekh, N., Steinhausen, M., Bührle, C. P., Nobiling, R., Ullrich, K. J., Rumrich, G., Klöss, S., Papavassiliou, F., Hoyer, J., Schmitt, C., Jungwirth, A., Ritter, M., Westphale, H. J., Bevan, C., Theiss, C., Denek, Liliana, Schwegler, Johann S., SchÄfer, Roland, Augustin, Albert J., Heidland, August, Nafz, B., Just, A., Steidl, M., Pinggera, G., Gerstberger, R., Schütz, H., Simon, E., Lohrmann, E., Masereel, B., Delarge, J., Lang, H. J., Englert, H. C., Caliebe, D., Mályusz, M., Wrigge, P., Gronow, G., Klause, N., Mályusz, M., Zinnert, H., Fagel, H., Jelkmann, W., Weiss, Ch., Augustin, A. J., Keil, R., Schmidt, W., Kröger, C., Brabant, E. G., Hilgendorf, A., Strauch, S., Lane, F., Prick, A., Golenhofen, N., Mildenberger, S., Schwegler, J. S., Flemming, B., Roloff, D., Wronski, T., Drews, G., Debuyser, A., Henquin, J. C., Jackson, M. B., DeRiemer, S. A., Schmid, A., Schnefel, S., Pröfrock, A., Hinsch, K. -D., Milz, J., Lamprecht, G., Seidler, U., Silen, W., Aziz, O., Reschke, W., Fischer, G., De Decker, N., Hayes, T., Coast, G., Van Kerkhove, E., von zur Mühlen, F., Eckstein, F., Hegel, U, Bentzel, CJ, Riecken, EO, Siemer, C., Rothenpieler, P., Smith, E., Lutnicki, K. R., Wróbel, J. T., Ledwożyw, A., PietraŚ, E., Sender, S., Jürgens, Klaus D., Kleinschmidt, T., Werkmeister, F., Kiwull-Schöne, H., Kiwull, P., Vahle, J., Ott, M., Zimmermann, R. E., Elsing, J. G., Million, D., Zillner, P., Thiel, M., Bardenheuer, H., Peter, K., Fandrey, J., Siegers, C. P., Rupp, H., Elimban, V., Dhalla, N. S., Morano, I., Agostini, B., Mühleisen, M., Mommaerts, W. F. H. M., Ono, K., Wussling, M., Schenk, W., Boldt, W., Lipp, P., Schüttler, K., Szymanski, G., Wendt-Gallitelli, M. F., Herzig, J. W., Depersin, H., Grupp, G., Grupp, I., Glitsch, H. G., Pusch, H., Zylka, Ch., Brāndle, M., Jacob, R., Stein, T., Isselhard, W., Sturz, J., Minor, T., Wingenfeld, P., Siegmund, B., Klietz, T., Schwartz, P., Piper, H. M., Linder, Christa, SchÄfer, Stefan, Heusch, Gerd, Becker, B. F., Reinholz, N., Raschke, P., Leipert, B., Gerlach, E., Dierberger, B., Gülch, R. W., Leverkus, M., Mitsuiye, T., Pohl, U., Wang, S. Y., Meyer, R., Haas, H. G., Christmann, H. Ph, Dörner, Th, Hock, D., Hertel, R., Gagelmann, M., Forssmann, W. G., Leijendekker, W. J., Kissling, G., Michel, H., Goetz, A., Freya, M., Fleckenstein-Grün, G., Schipke, Jochen D., Harasawa, Yasuhiko, Sugiura, Seiryo, Alexander, Joe, Burkhoff, Daniel, Kling, L., Müller-Beckmann, B., Schroth, M., Sponer, G., Böhm, E., Strein, K., Dorszewski, A., Arnold, G., Pike, G. K., Bryant, D. J., Roberts, M. L., Fink, R. H., Ross, Ch., Skyschally, A., Schulz, R., Linder, C., Heusch, G., Schipke, J. D., Burkhoff, D., Alexander, J., Gollnick, F., Peter, Kh., Franken-Weyers, R., Borst, M. M., Deussen, A., Pöpping, S., Hose, H., Strotmann, K. H., Lukascek, B., Karnath, T., Güttier, K., Klaus, W., Haverkampf, K., Guhlmann, M., Schmidt-Ott, S., Heuschen, U., Mall, G., Pfitzer, G., Rösch, J., Arner, A., Rüegg, J. C., Kröger, K., Schipke, J. D., ThÄmer, V., Ehring, Thomas, ThÄmer, Volker, Guth, B. D., Schnabel, Ph A., Schmiedl, A., Gebhard, M. M., Richter, J., Bretschneider, H. J., Guth, B. D., Oudiz, R. J., Schnabel, Ph., Richter, J ., Watanabe, H., Spahr, R., Piper, H. M., Obst, O., Mertens, H., Mülsch, A., Busse, R., Lamontagne, D., Herlan, K., Huang, A., Bassenae, E., Mackert, J. R. L., Schilling, L., Parsons, A. A., Wahl, M., Hock, D., Christmann, M. Ph., Thimm, F., Frey, M., Fleckenstein, a. A., Theilen, H., Göbel, U., Kuschinsky, W., Elbert, Th., Tafil-Klawe, M., Rau, H., Lutzenberger, W., Fleckenstein, A., Forst, H., Haller, M., Santjohanser, C., Lauterjung, L., Smieško, Y., Lang, D. J., Johnson, P. C., Schröck, H., Rau, H., Elbert, T., Geiger, B, Lutzenberger, W., Koch, G., Koralewski, H. E., Perschel, F. H., Wagner, K., Krüger, U., Albrecht, M., Hohlbach, G., Maassen, N., Foerster, M., Mühling, J., Bari, F., Pleschka, K., Schmidt, H. D., Gro\, H., Loock, W., Stick, C., Diefenbacher, U., Gronewold, D., Tobinsky, M., Walther-Behrends, A., Witzleb, E., Brummermann, M., Reinertsen, R. E., Rogausch, H., Rohn, W. M., Acker, H., Delpiano, M., Dufau, E., Hentschel, J., Heller, H., Schuster, K. -D., Siekmeier, R., Kronenberger, H., Lintl, H., Schiller-Scotland, Ch. F., Gebhart, J., Heyder, J., Meier-Sydow, J., Stahlhofen, W., Mottaghy, K., Geisen, C., Richter, W., Beckman, J., Marek, W., Ulmer, W. T., Thiele, A. E., Raschke, F., Peter, J. H., Hildebrandt, G., Kullmer, T., Kozianka-Burghof, G., Thiele, A. E., Schlaefke, M. E., Gnuschke, H., Schaefer, T., Schaefer, D., Schaefer, C., Bradley, Ronald J., Sterz, Raimund, Peper, Klaus, Benterbusch, R., Kraft, Th., Yu, L. C., Kuhn, H. J., Blankenbach, K., Asmussen, G., Kunze, I., Pieper, K. -S., Steinmetz, J., Schmidt, H., Krippeit-Drews, P., Hübschen, U., Nacimiento, A. C., Günzel, D., Rathmayer, W., Gaunitz, U., Költgen, D., Zachar, E., Soltau, B., De Martino, L., Hasselbach, W., Kössler, F., Lange, P., Küchler, G., Zeugner, C., Van Eyk, J., Hodges, R. S., Lorkovic, H., Clemens, N., Scheid, P., Noack, Th., Deitmer, P., Golenhofen, K., Lammel, E., Welling, Andrea, Felbel, Jochen, Hofmann, Franz, Katoch, S., Watanabe, T., Mandrek, K., Milenov, K., Hammer, K., Rössler, W., Sann, H., Pierau, Fr -K., Nguyen-Duong, H., Schneider, P., Stahl, F., Lepple-Wienhues, A., Korbmacher, C., Haller, H., Gebauer, M., Willner, U., Bialojan, C., Lengsfeld, M., Kyrtatas, V., Dartsch, Peter C., Boels, P. J., Fischer, W., Lenz, T., Thei\, U., Kreye, V. A. W., Ohkubo, T., Kupp, H., Vonderlage, M., Schreiner, V., Dorlöchter, M., Brinkers, M., Irintchev, A., Wernig, A., Langenfeld, B., Finger, W., Wolburg, H., Beer, A., Schwejda, Ch., Scheller, D., Heister, U., Tegtmeier, F., Knöpfel, Thomas, Spuler, Andreas, Grafe, Peter, GÄhwiler, Beat, Bijak, M., Misgeld, U., Müller, W., Rausche, G., Leweke, F M., Bingmann, D., Moraidis, I., Speckmann, E. -J., Madeja, M., Mu\hoff, U., Lehmenkühler, A, Kuhlmann, D., Hans, M., Lux, H. D., StrÄub, H., Waiden, J., Baker, R. E., Grantyn, R., Perouansky, M., Kraszewski, K, Lehmenkühler, Chr, Dodt, H. U., ZieglgÄnsberger, W., Pawelzik, H., ZieglgÄngsberger, W., Mann, K., Wiethölter, H., Albrecht, D., Dreier, J., Ficker, E., Beck, H., Corrette, B J., Dreyer, F., Repp, H., Dreessen, J., Augustine, G. J., Lehmenkühler, A., Büsselberg, D., Heimrich, B., Haas, H. L., Birnstiel, S., Haas, H. L., Schönrock, B., Altrup, U., Reith, H., Speckmann, E. -J., Alzheimer, C., Bruagencate, G. ten, Fruhstorfer, B., Mignot, E., Nishino, S., Dement, W. C., Guilleminault, C., Simon-Oppermann, Christa, Günther, Olaf, Stehle, J., Reuss, S., Seidel, A., Riemann, R., Vollrath, L., Reimer, Susanne, HölIt, Volker, Sonnhof, U., Krupp, J., Claus, H, Hinckel, P., Dick, H. B. H., Hiemke, C., Jussofie, A., Dorn, T., Uhlig, S., Witte, O. W., Bother, B., Eiselt, M., Witte, H., Zwiener, ö, Rother, M, Eiseit, H., Taghavy, A., KrÄtzer, A., Clusmann, H., Heinemann, U., Block, F., Sonatg, K. -H., Falkeristein, M., Hohnsbein, J., Hoormann, J., Frieling, A., Tarkka, I. M., Kullmann, W., Bromm, B., Hirsch, M. Chr, Wissing, H., Braun, H. A., Igelmund, P., Klu\mann, F. W., Ehrenstein, W. H., Yakimoff, N., Mateeff, S., Zeise, M. L., Arriagada, J., Teschemacher, A., ZieglgÄnsberger, W., Pöppelmann, T., Köhling, R., Boerrigter, P., Reith, H., Anders, K., Ohndorf, W., Dermietzel, R., Richter, D. W., Tölle, T. R., Castro-Lopes, J. M., Neuropharmakologie, Klinische, Sandkühler, J., Leah, J. D., Herdegen, T., Zimmermann, M., Vaitl, D., Gnippe, H., Herbert, M. K., Mengel, M. K. C., Kniffki, K. -D., Linke, R., Vahle-Hinz, C., Schenda, J., Matsumura, K., Herdegen, T., fu, Q. -G., Forster, C., Hutchison, W. D., Morton, C. R., Aschoff, J., Wilhelm, Z., Schwarzacher, S. W., Wasserschaff, M, Hörner, M., Kümmel, H., Windhorst, U., Feldman, J. L., Schmid, K., Foutz, A. S., Denavit-Saubié, M., Pak, M. A., Wehling, P., Evans, C., Bandara, G., Awiszus, F., Feistner, H., Heinze, H. -J., Illert, M., Wasserschaff, M., Kleinebeckel, D., Böhmer, G., Schauer, W., Abel, H. -H., Klü\endorf, D., Koepchen, H. P., Jarolimek, W, König, St, Czachurski, J., Seller, H., Meckler, R. L., McLachlan, E. M., Boczek-Funcke, A., HÄbler, H. -J., JÄnig, W., Michaelis, M., Dembowsky, K., Königr, S., Rau, Harald, HÄbler, H. -J., Unger, M., Merker, G., Roth, J., Zeisberger, E., Gao, H., Hunold, M., Kirchner, F., Takano, K., Schulze, K., Pokorski, M., Sakakibara, Y., Masuda, A., Morikawa, T., Ahn, B., Takaishi, S., Paulev, P. -E., Honda, Y., Flügge, G., Fuchs, E., König, S., Eysel, U. Th., Schmidt-Kastner, R., Skrandies, W., Geib, T., Baumann, C., Schmidt, K. -F., Knapp, A. G., Dowling, J. E., Kuba, M., Toyonaga, N., Kubová, Z., Ehrenstein, W. H., Jacobi, P., Schmidt, K. -F., Nöll, G. N., Baumann, Ch., Tabata, M., Martin, Ch., Meissl, H., Knottenberg, Th., Scheibner, H., Zenner, Hans P., Zimmennann, Ulrike, Gitter, Alfred H., Ding, D., Smolders, J. W. T., Klinke, R., Boekhoff, I., Raming, K., Krieger, J., Tareilus, E., Strotinann, J., Breer, H., Schild, D., DeSimone, J. A., Hellwig, S., Gitter, A. H., Plinkert, P. K., Zenner, H. P., Koltzenbwg, M., Pinter, E., SchÄfer, K., Braun, H. A., Necker, R., Hanesch, U., Heppelmann, B., Schmidt, R. F., Mense, S., Hoheisel, U., Steen, K. H., Anton, F., Reek, P. W., Handwerker, H. O., Lewin, G. R., McMahon, S. B., Heyer, G., Hornstein, O. P., Klement, W., Arndt, J. O., Maeerl, W., GrÄmer, G., Schepelmann, K., Me\linger, K., Schaible, H. -G., Treede, R. D., Meyer, R. A., Campbell, J. N., Claus, D., Neundörfer, B., Ernst, R., Tick-Waider, A. M., Bretschneider, F., Peters, R. C., Tennis, P. F. M., Teunis, P. F. M., Hoheisel, D., Scherotzke, R., Bub, A., Manzl, G., Forssmann, W. G., Jessen, C., Nuesslein, B., Schmidt, I., Wetzig, J., Reiser, M., Bregenzer, N., von Baumgarten, R. J., Mohr, E., Krzywanek, H., Warncke, G., Schuchmann, K. -L., Linow, H., Klu\mann, F. H., Redlin, U., Heldmaier, G., Bamler, A, Koller, A., Felber, S., Haid, C., Wicke, K., Raas, E., Xuemin, Wang, Kerning, Chen, Ying, Shi, Hanping, Shi, Warncke, Günther, Voisord, R., Dortsch, P. C., Betz, E., Karbach, U., Walenta, S., Gross, M. W., Mueller-Klieser, W., Vaupel, P., Okunieff, P., Mayer, W. -K., Stohrer, M., Krüger, W., Müller-Klieser, W., Strupp, M., Weial, P., Bostock, H., Piwernetz, K., Renner, R., Grafe, P., Lankers, J., Zangemeister, W., Kunze, K., Tries, S., Heinle, H., Beckerath, N. V., Maier-Rudolph, W., Mehrke, G., Günther, K., Goedel-Meinen, L., Daut, J., Piper, H. M., Kopp, A., Noll, T., Goellner, A., Gerlach, S., Teutsch, H. F., Schienger, K., Schwab, R., Höckel, M., Fotev, Z., Nienhaus, M., Kaczmarczyk, Gabriele, Richter, Dinah, Korte, Gabriele, Förther, J., Reinhardt, H. W., Schreiber, R., Rupp, J., Murphy, G., Fingerle, J., Kloiber, O., Miyazawa, T., Höhn-Berlage, M., Hossmann, K. -A., Schad, H., Heimisch, W., Blasini, R., Haas, F., Mendier, M., Spuler, A., Lehmann-Hom, F., Wolfram, U., Fenske, M., Sachser, N., Weis, Ch., Marktl, W., Kopta, B., Klammer, N., Rudas, B., Pohl, H., Nienartowicz, A., Moll, W., Klempt, M., Blum, S., Bühler, H., Lichtenstein, I., Novak, A., Siebe, H., Hierholzer, K., and Peper, K.
- Published
- 1990
- Full Text
- View/download PDF
29. Inhibition of nitric oxide synthesis in ischemia/reperfusion of the rat liver is followed by impairment of hepatic microvascular blood flow
- Author
-
Koeppel, T. A., Thies, J. C., Schemmer, P., Trauner, M., Gebhard, M.-M., Otto, G., and Post, S.
- Published
- 1997
- Full Text
- View/download PDF
30. Continuous Measurement of Porcine Renal Cortex Microcirculation with Enhanced Thermal Diffusion Technology
- Author
-
Kraus, T., Klar, E., Osswald, B. R., Fernandes, L., Mehrabi, A., Gebhard, M. M., and Herfarth, C.
- Abstract
Continuous monitoring of renal cortical blood flow (RCBF) in the perioperative setting of aortic or renal vascular surgery could faciliate the early detection of vascular complications, possibly resulting in a reduction of postoperative renal failure. A new prototype system for measurement of parenchymous organ perfusion based on the principle of thermal diffusion (“TD”—Thermal Diffusion Electrode, Thermal Technologies Inc., Cambridge, MA, USA) was used for RCBF measurements in the outer cortex of the porcine kidney. We validated the sensitivity of the device to detect renal blood flow impairment, comparing TD flow data with renal artery blood flow values (RABF), measured by ultrasonic flow probes. The hypothesis was tested that acute disturbances of RCBF, induced by a variable degree of renal artery stenosis, can be immediately detected and continuously monitored by TD measurements in the porcine renal cortex. Mean baseline RCBF measured by TD electrodes was 68.1 ± 25.0 ml/100 g/min. Mean baseline RABF was 102.1 ± 26.6 ml/min. Controlled induction of a variable degree of renal arterial occlusion by implanted vascular balloon occluders was always followed by an immediate and proportional decline of RCBF, as measured by TD. Flow data obtained with both methods were significantly correlated by linear regression (r= .82,r2= .68;P< 0.0001). Dynamic changes of RABF in the time course of renal artery partial/total occlusion and arterial flow release could be continuously followed by detection of corresponding flow changes of RCBF. We conclude that the TD system investigated in the current study allows a continuous and sensitive determination of porcine renal cortex perfusion. A clinical evaluation of the method, e.g., in the perioperative setting of aortic or renal transplantation surgery, now appears to be justified.
- Published
- 1996
- Full Text
- View/download PDF
31. Comparison of histidine-tryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution for organ preservation in human liver transplantation
- Author
-
Erhard, J., Lange, R., Scherer, R., Kox, W. J., Bretschneider, H. J., Gebhard, M. M., and Eigler, F. W.
- Abstract
Over a 30-month period, 60 patients (30 in each group) suffering from end-stage liver disease or primary hepatic malignancy and scheduled for liver transplantation were enrolled in a prospective, randomized study to compare two methods of liver preservation: histidinetryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution. Entry criteria for both groups were: age (18–65 years), elective surgery (transplantable or urgent category of the recipients), first transplantations and harvesting procedure performed by the same team. The parameters under investigation were the clinical and laboratory data preand post-transplantation, as well as follow-up data such as complications and survival. There were no significant differences in the two groups as far as the evaluation criteria were concerned, even when cold ischemia time was more than 15h (n=7). A slight, yet not significant, increase in late complications of the biliary anastomoses could be seen in the UW group. Hepatocellular injury (SGOT, SGPT, GLDH, lactate) appeared to be more marked in the HTK group. These results suggest that both HTK and UW solutions are appropriate for clinical use in liver transplantation, even if cold ischemia time is more than 15h.
- Published
- 1994
- Full Text
- View/download PDF
32. Combination of Deep Level Transient Spectroscopy and Transmutation of Radioactive Impurities
- Author
-
Pensl, Gerhard, Lang, M., Gebhard, M., Achtziger, N., and Uhrmacher, M.
- Abstract
Not Available
- Published
- 1992
- Full Text
- View/download PDF
33. Interruption of bronchial circulation leads to a severe decrease in peribronchial oxygen tension in standard lung transplantation technique.
- Author
-
Herold, U, Jakob, H, Kamler, M, Thiele, R, Tochtermann, U, Weinmann, J, Motsch, J, Gebhard, M M, and Hagl, S
- Abstract
In clinical practice lung transplantation is the only procedure where the transplanted organ is left without its own arterial perfusion. With the interruption of the bronchial arteries the nutritive support is dependent on collateral flow by the pulmonary artery and the oxygen tension of desaturated central venous blood, representing an abnormal physiology.
- Published
- 1998
- Full Text
- View/download PDF
34. Direct visualization of leukocyte/endothelial cell interaction during extracorporeal circulation (ECC) in a new animal model.
- Author
-
Kamler, M, Jakob, H, Lehr, H A, Gebhard, M M, and Hagl, S
- Abstract
The clinical complications of extracorporeal circulation (ECC) have been linked to a systemic activation of cellular and humoral components and to a dysregulation of the microcirculatory compartment. Since to date only in vitro methods exist for evaluation, we developed an animal model to study the effects of ECC on the microcirculation. To establish the model, we assessed whether these effects are dependent on the duration of ECC.
- Published
- 1997
- Full Text
- View/download PDF
35. Different Effects of Cardioplegic Solution HTK during Single or Intermittent Administration
- Author
-
Gebhard, M. M., Preusse, C. J., Schnabel, Ph. A., and Bretschneider, H. J.
- Published
- 1984
- Full Text
- View/download PDF
36. Myocardial “Equilibration Processes” and Myocardial Energy Turnover during Initiation of Artificial Cardiac Arrest with Cardioplegic Solution - Reasons for a Sufficiently Long Cardioplegic Perfusion
- Author
-
Preusse, C. J., Gebhard, M. M., and Bretschneider, H. J.
- Published
- 1981
- Full Text
- View/download PDF
37. Intracellular pH, Na+- and K+-Activities at the Onset of St. Thomas' Cardioplegia: A Study with Ionselective Microelectrodes
- Author
-
Stinner, B., Krohn, E., Gebhard, M. M., and Bretschneider, H. J.
- Published
- 1988
- Full Text
- View/download PDF
38. Myocardial Protection: Left Ventricular Ultrastructure after Different Forms of Cardiac Arrest
- Author
-
Schnabel, Ph. A., Gebhard, M. M., Pomykaj, Th., Schmiedl, A., Preuße, C. J., Richter, J., and Bretschneider, H. J.
- Published
- 1987
- Full Text
- View/download PDF
39. Interstitial pH value in the myocardium as indicator of ischemic stress of cardioplegically arrested hearts
- Author
-
Preusse, C. J., Gebhard, M. M., and Bretschneider, H. J.
- Abstract
Summary The influence of different cardioplegic methods (Bretschneider, Cardiac Surgery in Hamburg, Kirklin, St. Thomas' Hospital in London) on the progressive myocardial acidosis during global ischemia of up to 24 hours' duration was investigated in arrested, nonperfused canine hearts (n=36). The increasing acidosis of the ischemic heart muscle was continuously measured and registered with the aid of interstitial pH measurement (pH
i ) using glass implant electrodes. With various buffered and unbuffered cardioplegic solutions there were relatively wide fluctuations of the pHi courses in the myocardial extracellular space. The “critical” interstitial pH values (“pHcrit ”) relative to a defined myocardial ATP concentration (4 μmol/g ww) vary between 6.10 (Bretschneider) and 5.6 (Kirklin) (p<0.001). These striking results cannot be attributed solely to different total myocardial lactate concentrations, but variations in a “cellular” and an “extracellular factor” must also be taken into account.- Published
- 1982
- Full Text
- View/download PDF
40. Functional alterations of cardiac subcellular structures during energy deficiency in relation to the metabolic state of the heart muscle cell
- Author
-
Spieckermann, P. G., Gebhard, M. M., Göring, G. G., Kahles, H., Mezger, V. A., Preuße, C. J., and Stellwaag, M.
- Abstract
Summary The functional behaviour of membrane systems of the cardiac cell during oxygen deficiency was analyzed and the alterations were related to the metabolic state of the tissue as an index of injury.1.The retention function of the cell membrane for proteins. With increasing energy deficiency the cardiac sarcolemma loses its ability to retain macromolecules (myoglobin, enzymes) within the cell. Close correlations exist between protein release and oxygen supply as well as ATP content of the tissue.2.Function of isolated mitochondria after ischemia. In parallel with a strong impairment of oxidative phosphorylation (decrease of Q
O 2 , RCI values, phosphorylation rates) the Ca++ -transporting activity of mitochondria is continuously depressed with decreasing myocardial ATP.3.Function of isolated sarcoplasmic reticulum after ischemia. With breakdown of high energy phosphates during ischemia rate and extent of Ca++ binding both decrease markedly.- Published
- 1980
- Full Text
- View/download PDF
41. Influence of N-acetylcysteine treatment on endotoxin-induced microcirculatory disturbances
- Author
-
Schmidt, W., Walther, A., Martin, E., Schmidt, H., and Gebhard, M. M.
- Abstract
Objectives: To determine the influence of N-acetylcysteine (NAC) in a treatment model, its effects on endotoxin-induced leukocyte-endothelial cell adhesion, vascular leakage, and venular micro-hemodynamics in postcapillary venules of rat mesentery. Design: Prospective, randomized, controlled, experimental study. Setting: Animal research laboratory. Subjects: 40 male Wistar rats. Interventions: The rats randomly received one of four treatments: infusion of saline (SAL) or Escherichia colilipopolysaccharides (LPS) followed by treatment with saline (SAL) or NAC (150 mg · kg
− body weight) 30 min after induction of endotoxemia. Measurements and main results: Leukocyte adherence, red blood cell velocity, and vessel diameters in postcapillary venules of rat mesentery were evaluated every 30 min over a period of 120 min using in vivo videomicroscopy. Vascular permeability was determined by measuring the extravasation of fluorescence-labeled albumin. Venular wall shear rate was calculated from red cell velocity, and vessel diameter. NAC in rats without endotoxemia (SAL + NAC group) compared to the control group (SAL + SAL) did not change microcirculatory parameters in postcapillary venules of rat mesentery. In both LPS-treated groups (LPS + SAL and LPS + NAC), leukocyte adherence increased after just 30 min. NAC treatment prevented a further increase in leukocyte adherence and attenuated the extravasation of fluorescence-labeled albumin during endotoxemia. Venular diameters remained unchanged, while erythrocyte velocity decreased in the LPS + SAL group. This led to a lower venular wall shear rate in this group. Conclusions: Treatment with NAC attenuates endotoxin-induced leukocyte adherence and macromole-cular leakage in postcapillary venules of rat mesentery, showing that NAC is also effective after the onset of endotoxemia.- Published
- 1998
- Full Text
- View/download PDF
42. Intracellular sodium activity and Bretschneider's cardioplegia: Continuous measurement by ionselective microelectrodes at initial equilibration
- Author
-
Stinner, B., Krohn, E., Gebhard, M. M., and Bretschneider, H. J.
- Abstract
Summary Intracellular sodium activity (a
Na i ), intracellular pH (pHi ) and membrane potential were directly and continuously measured in sheep cardiac Purkinje fibers using neutral carrier liquid membrane ionselective microelectrodes. Changing the superfusing medium from normal Tyrode's solution to the cardioplegic solution “HTK” according to Bretschneider (6) a depolarization from −73.7±7.2 mV to −55.0±9.5 mV (n=25), a decrease of aNa i from 9.1±1.9 mM to 4.0±1.4 mM (n=25) and an intracellular acidification from pHi 7.18±0.06 to pHi 7.01±0.06 (n=11, x±S.D.) occurred at 35°C. The decrease of intracellular sodium activity was not effected by replacement of K, Mg, or histidine by mannitol in the cardioplegic solution. Addition of 4 mM Ca somewhat enhanced aNa i decline. Inhibition of the sodium pump with the cardiac steroid dihydroouabain (10−4 M) lowered the effect of “HTK” on intracellular sodium by approximately 35% (n=5). Sodium decline was also sensitive to equilibration temperature, giving a Q10 of 1.54 for the initial decrease velocity (temperature range 20 to 35°C), which is less than that found by other investigators for pure sodium pump activity. It is suggested that although the electrochemical sodium gradient remains inward throughout, sodium may leave myocardial cells on induction of Bretschneider's cardioplegia because of a reduction of inward fluxes by simultaneously increasing sodium pump activity, thus increasing Na efflux. Na/Ca exchange is assumed to be of minor importance and the Na/H exchange may be involved. With respect to the clinical application of the low Na and nominally Ca-free cardioplegic solution “HTK” lowering of intracellular sodium activity is interpreted as a factor minimizing the risk of a “calcium paradox” on reperfusion with Ca at serum levels, as well as a possible mechanism preventing early development of cellular edema.- Published
- 1989
- Full Text
- View/download PDF
43. The role of ATP and lactic acid for mitochondrial function during myocardial ischemia
- Author
-
Kahles, H., Gebhard, M. M., Mezger, V. A., Nordbeck, H., Preuße, C. J., and Spieckermann, P. G.
- Abstract
Summary Phosphorylation rates of canine heart mitochondria isolated after various periods of myocardial ischemia after cardioplegic arrest were correlated with the myocardial ATP-, lactate- and undissociated lactic acid content as well as with interstitial H
+ -concentration. The following correlation coefficients were found: ATP: 0.87, lactate: 0.93, interstitial H+ : 0.73. The calculated undissociated lactic acid content and the mitochondrial phosphorylation rate during ischemia showed a correlation coefficient of r=0.95.- Published
- 1979
- Full Text
- View/download PDF
44. Investigation of cadmium-hydrogen complexes in silicon
- Author
-
Gebhard, M., Achtziger, N., Baurichter, A., Forkel, D., Vogt, B., and Witthuhn, W.
- Abstract
Formation, stability and electric properties of (In-H) and (Cd-H) complexes in Si are studied by the perturbed angular correlation spectroscopy (PAC). The trapping of hydrogen at the acceptors results in three different defect specific electric field gradients (EFGs), which are studied as function of temperature, doping concentration and annealing time. Two of the three observed hydrogen-related EFGs are identified as different charge states of one (Cd-H) acceptor. Within the framework of a model, which takes into account the dynamics of charge fluctuations and Shockley, Read and Hall statistics, the energy level of this (Cd-H) acceptor at E= EV+ 60 meV is deduced. Furthermore, the dynamical behavior of the hydrogen atom within the (Cd-H) complexes is studied. From the relaxation of the PAC signal the hopping rate of the hydrogen atom is extracted. This rate is thermally activated with an activation energy of E= 0.20 eV.
- Published
- 1991
- Full Text
- View/download PDF
45. Influence of glycogen content, temperature, and Euro Collins solution on membrane potential and sodium activity of superfused porcine liver slices
- Author
-
Fleckenstein, M., Kehrer, G., Gebhard, M., and Bretschneider, H.
- Abstract
Summary: The influence of glycogen content temperature, and Euro Collins (EC) solution on membrane potential (V
m ) and intracellular sodium activity (aNa i ) were measured in cells of superfused porcine liver slices by means of double-barrelled ion-sensitive microelectrodes. Vm was −26.1 mV in fasted pigs and −20.6 mV after glucose feeding, when measured in HEPES-buffered solution (P<0.0001). aNa i was not measurably affected by glucose feeding. During superfusion with Tyrode solution, lowering the temperature from 35.5C to 15.5C led to a fast Vm decrease of roughly 2 mV followed by an increase of 1–3 mV. At the same time, aNa i increased from 12.8 to 18.2 mM within 10min. Superfusion with EC solution for 10 min caused comparable changes in fed and fasted pigs. Vm depolarized at either temperature by about 16 mV. At 35.5C the initial aNa i of 17.5 mM was roughly halved, whereas at 15.5C it decreased from 21.0 to 14.3 mM. The results suggest that the nutritional state markedly affects the electric properties of liver. However, the effect on membrane potential of high-potassium organ-protective solutions seems to be distinctly more pronounced. Moreover, cellular Na+ activity decreases in consequence of an extracellular Na+ reduction with protective solutions which might be balanced to some extent by a simultaneous temperature decrease.- Published
- 1991
- Full Text
- View/download PDF
46. Hydrogen in Nb — Hf — Nb Layered Structures*
- Author
-
Ziegler, P., Sheng, Y.Q., Gebhard, M., Boebel, O., and Weidinger, A.
- Published
- 1933
- Full Text
- View/download PDF
47. Reduction of hepatic reperfusion injury by antithrombin III and aprotinin
- Author
-
Maksan, S.‐M., Maksan, M.‐O., Gebhard, M.‐M., Herfarth, C., and Klar, E.
- Abstract
AbstractDisturbance in hepatic microcirculation and leucocyte‐endothelium interaction after warm ischaemia represents one of the leading mechanisms for postoperative organ dysfunction. Recent studies have shown that pretreatment with antithrombin III (AT III) and aprotinin reduces the leucocyte‐endothelium interaction in ischaemic small intestine and during extracorporal circulation in cardiac surgery. Standardized warm hepatic ischaemia and intravital fluorescence videomicroscopy was performed in an experimental study with rats. Animals were pretreated with AT III or aprotinin. Analysis of intravital videomicroscopy showed that the hepatic microcirculation after warm hepatic ischaemia in rats was significantly enhanced by AT III and aprotinin, most likely by reducing the leucocyte‐endothelium interaction. We concluded that drug application before the Pringle manoeuvre might reduce the reperfusion damage after liver resection.
- Published
- 2000
- Full Text
- View/download PDF
48. Reduction of hepatic reperfusion injury by antithrombin III and aprotinin
- Author
-
Maksan, S.-M., Maksan, M.-O., Gebhard, M.-M., Herfarth, C., and Klar, E.
- Abstract
Abstract Disturbance in hepatic microcirculation and leucocyte-endothelium interaction after warm ischaemia represents one of the leading mechanisms for postoperative organ dysfunction. Recent studies have shown that pretreatment with antithrombin III (AT III) and aprotinin reduces the leucocyte-endothelium interaction in ischaemic small intestine and during extracorporal circulation in cardiac surgery. Standardized warm hepatic ischaemia and intravital fluorescence videomicroscopy was performed in an experimental study with rats. Animals were pretreated with AT III or aprotinin. Analysis of intravital videomicroscopy showed that the hepatic microcirculation after warm hepatic ischaemia in rats was significantly enhanced by AT III and aprotinin, most likely by reducing the leucocyte-endothelium interaction. We concluded that drug application before the Pringle manoeuvre might reduce the reperfusion damage after liver resection.- Published
- 2000
- Full Text
- View/download PDF
49. INFLUENCE OF A MODIFIED PRESERVATION SOLUTION IN PROLONGING THE COLD ISCHEMIC PERIOD – A COMPARATIVE STUDY IN A PORCINE MODEL OF ALLOGRAFT KIDNEY TRANSPLANTATION
- Author
-
Fonouni, H, Mehrabi, A, Kuttymuratov, G, Longerich, T, Golriz, M, Rad, M Tahmasbi, Esmaeilzadeh, M, Jarahian, P, Büchler, M W., Schmidt, J, and Gebhard, M M.
- Published
- 2008
- Full Text
- View/download PDF
50. Desmopressin Impairs Microcirculation in Donor Pancreas and Early Graft Function after Experimental Pancreas Transplantation. Transplantation 2001 72 202.
- Author
-
Keck, T, Banafsche, R, Werner, J, Gebhard, M-M, Herfarth, C, and Klar, E
- Published
- 2001
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.