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The aim of this work is to propose a new alternative method based on plants to fight against synthetic insecticides. The powder and the ethanolic extract, prepared from the leaves of Taxus baccata of Ifrane Val, were tested on Sitophilus oryzae (L.) with different concentrations and for various durations of exposure. On the first hand, the toxicity of the prepared powder and ethanolic extract increases with the concentration. On the second hand, it is noted that this toxicity increases with the duration of exposure of Sitophilus oryzae (L.). Given these results, the powder and ethanolic extract of the leaves of Taxus baccata can be recommended as a bioinsectide candidate against Sitophilus oryzae (L.) and as an alternative to synthetic agriculture pesticides. [ABSTRACT FROM AUTHOR]

This work focuses on the search for natural molecules with biological activity that can be used to find alternatives to synthetic products by studying the chemical composition and evaluating the antioxidant and antibacterial activity of the extract of Taxus baccata (L.). HPLC-UV chromatography was employed to determine its chemical composition, while the antioxidant activity was determined using DPPH radical scavenging method and cyclic voltammetry. The broth dilution method was used to achieve antibacterial activity against in total 14 different foodborne pathogenic bacteria, with 4 being Gram-positive and 10 being Gram-negative. The results of the analysis of the extract studied by HPLC-UV made it possible to identify about ten phenolic compounds. Salicylic acid (18.8 µg/mg), gallic acid (5.45 µg/mg), quercitin (4.8 µg/mg), rutin (3.98 µg/mg), hydroxybenzoic acid (3.34 µg/ mg), caffeine (1 µg/mg), caffeic acid (0.267 µg/mg) and vanillin (0.258 µg/mg). the antioxidant activity showed an interesting antiradical activity, the voltammogram also shows an oxidation peak relating to the oxidation of the phenolic compounds contained in Taxus baccata (L.). The antibacterial activity results show significant antibacterial properties against Gram-positive and Gram-negative strains. [ABSTRACT FROM AUTHOR]