1. Automated imaging analysis of Ki-67 immunohistochemistry on whole slide images of cell blocks from pancreatic neuroendocrine neoplasms.
- Author
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Shaker, Nada, Shen, Rulong, Limbach, Abberly Lott, Satturwar, Swati, Kobalka, Peter, Ahmadian, Saman, Sun, Shaoli, Chen, Wei, Lujan, Giovanni, Esnakula, Ashwini, Parwani, Anil, and Li, Zaibo
- Abstract
Accurate grading of pancreatic neuroendocrine tumors (PanNETs) relies on the assessment of Ki-67 immunohistochemistry (IHC). While digital imaging analysis (DIA) has been employed for Ki-67 IHC assessment in surgical specimens, its applicability to cytologic specimens remains underexplored. This study aimed to evaluate an automated DIA for assessing Ki-67 IHC on PanNET cell blocks. The study included 61 consecutive PanNETs and 5 pancreatic neuroendocrine carcinomas. Ki-67 IHC slides from cell blocks were digitally scanned into whole slide images using Philips IntelliSite Scanners and analyzed in batches using the Visiopharm Ki-67 App in a digital workflow. Ki-67 scores obtained through DIA were compared to pathologists' manual scores. The Pearson correlation coefficient of the percentage of Ki-67-stained nuclei between DIA reads and the originally reported reads was 0.9681. Concordance between DIA Ki-67 grades and pathologists' Ki-67 grades was observed in 92.4% (61/66) of cases with the calculated Cohen's Kappa coefficient of 0.862 (almost perfect agreement). Discordance between DIA and pathologists' consensus reads occurred in 5 PanNET cases which were upgraded from G1 to G2 by DIA due to contaminated Ki-67-stained inflammatory cells. DIA demonstrated excellent concordance with pathologists' assessments, with only minor grading discrepancies. However, the essential role of pathologists in confirming results is emphasized to enhance overall accuracy. • Digital imaging analysis (DIA) of cytology cell block Ki-67 immunohistochemistry (IHC) from pancreatic neuroendocrine tumors • Automated digital workflow to analyze Ki-67 IHC whole slide images • Excellent concordance between DIA and pathologists' consensus scoring of Ki-67 IHC [ABSTRACT FROM AUTHOR]
- Published
- 2024
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