1. A method for isolating and culturing placental cells from failed early equine pregnancies.
- Author
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Rose, B.V., Cabrera-Sharp, V., Firth, M.J., Barrelet, F.E., Bate, S., Cameron, I.J., Crabtree, J.R., Crowhurst, J., McGladdery, A.J., Neal, H., Pynn, J., Pynn, O.D., Smith, C., Wise, Z., Verheyen, K.L.P., Wathes, D.C., and de Mestre, A.M.
- Subjects
ANIMAL experimentation ,BLASTOCYST ,CELL culture ,CELL separation ,COMPARATIVE studies ,FETAL ultrasonic imaging ,GESTATIONAL age ,HORSES ,RESEARCH methodology ,MEDICAL cooperation ,MISCARRIAGE ,PLACENTA ,RESEARCH ,EVALUATION research - Abstract
Early pregnancy loss occurs in 6-10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14-65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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