1. Exosomal miRNA Profiling in Lebanese Multiple Sclerosis Patients.
- Author
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Matar, Alaa, El-Hachem, Nehme, Marchand, Virginie, and Borjac, Jamilah
- Abstract
Objective: Recent studies shed the light about the implication of exosomes as biomarkers in various diseases. In this preliminary study, we investigated the serum exosomal microRNA (miRNA) profiles in relapsing- remitting multiple sclerosis (RRMS) patients and healthy subjects from the Bekaa region of Lebanon. Methods: Next generation sequencing (NGS) technique was used to establish the serum exosomal miRNA profiles in 3 RRMS patients in the remission state and 3 healthy subjects. Near to 1250 miRNAs were detected in all exosomal samples with a yield of 13 to 14 million reads. Bioinformatics analysis consisted of detailed analysis of differentially expressed miRNAs as well as target genes predicted. Results: Data analysis revealed that 12 miRNAs were differentially expressed between RRMS patients and controls. Among them, 6 were upregulated (miR-330-5p, let-7i-5p, miR-3529-3p, miR-182-5p, miR-3184-5p and miR-7-5p, and 6 were down regulated (miR-223-3p, miR-199b-5p, miR-542-3p, miR-582-5p, miR-520c-5p and miR-7641) in RRMS patients in comparison to controls. Conclusion: Our data support the evidence that circulating exosomal miRNAs may serve as powerful biomarkers in MS especially in discriminating between RRMS patients and controls. [ABSTRACT FROM AUTHOR]
- Published
- 2022