55 results on '"Das, Surajit"'
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2. Switchable Bulk Photovoltaic Effect in Intrinsically Ferroelectric 3D All-Inorganic CsPbBr3Perovskite Nanocrystals
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Ghosh, Anashmita, Paul, Susmita, Das, Mrinmay, Sarkar, Piyush Kanti, Bhardwaj, Pooja, Sheet, Goutam, Das, Surajit, Kalimuddin, Sk, Datta, Anuja, and Acharya, Somobrata
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Ferroelectric all-inorganic halide perovskite nanocrystals with both spontaneous polarization and visible light absorption are promising candidates for designing ferroelectric photovoltaic applications. It remains a challenge to realize ferroelectric photovoltaic devices with all-inorganic halide perovskites that can be operated in the absence of an external electric field. Here we report that a popular all-inorganic halide perovskite nanocrystal, CsPbBr3, exhibits a ferroelectricity-driven photovoltaic effect under visible light in the absence of an external electric field. Pristine CsPbBr3nanocrystals exhibit intrinsic ferroelectric key properties with a notable saturated polarization of ∼0.15 μC/cm2and a high Curie temperature of 462 K, driven by the stereochemical activity of the Pb(II) lone pair. Furthermore, application of an external electric field allows the photovoltaic effect to be enhanced and the spontaneous polarization to be switched with the direction of the electric field. CsPbBr3nanocrystals exhibit a robust fatigue performance and a prolonged photoresponse under continuous illumination in the absence of an external electric field. These findings establish all-inorganic halide perovskite nanocrystals as potential candidates for designing photoferroelectric devices by coupling optical functionalities and ferroelectric responses.
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- 2024
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3. Advancements in metrology for advanced semiconductor packaging
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de Groot, Peter J., Guzman, Felipe, Picart, Pascal, Chein, Wei-Hsin, Pandey, Gaurav, Das, Surajit, and Chen, Liang-Chia
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- 2024
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4. Expediency of Tetra- and Pentanucleotide Repeat Autosomal STR Markers for DNA Typing in Central Indian Population
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Dash, Hirak R., Shrivastava, Pankaj, and Das, Surajit
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The STR typing has been regarded as the most reliable and robust genetic tool having a pivotal role in the criminal justice system. High mutation rates have led to widespread polymorphism in the STRs which increases the probability of divergence of a specific population. Therefore, two tetra- and two pentanucleotide repeat STR loci were evaluated for their informativeness and expediency in the central Indian population. Two hundred unrelated individuals of the central India were included in this study for DNA isolation followed by multiplex PCR and genotyping using four STR markers, i.e., D2S1338, D19S433, Penta E and Penta D. All STR markers followed the Hardy–Weinberg equilibrium in the studied population. Out of all, Penta E (15p15-39; AAAGA) was found to be the most polymorphic (25–120 bp), highest heterozygosity (92.0%), lowest matching probability (0.025), highest power of discrimination (0.975), polymorphic information content (PIC) (0.91), power of exclusion (0.936) and typical paternity index (6.25). The aptness of the Penta E STR marker in context to its forensic and paternity parameters strongly suggests its applicability in the central Indian population.
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- 2024
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5. High-Performance Paper-Based Thermoelectric Generator from Cu2SnS3 Nanocubes and Bulk-Traced Bismuth.
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Das, Surajit, Mondal, Bhargab P., Ranjan, Priya, and Datta, Anuja
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- 2023
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6. High-Performance Paper-Based Thermoelectric Generator from Cu2SnS3Nanocubes and Bulk-Traced Bismuth
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Das, Surajit, Mondal, Bhargab P., Ranjan, Priya, and Datta, Anuja
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Flexible paper-based thermoelectric generators (PTEGs) have drawn significant interest in recent years due to their various advantages such as flexibility, adaptability, environment friendliness, low cost, and easy fabrication process. However, the reported PTEG’s output performance still lags behind the performance of other flexible devices as it is not so easy to obtain a compact film on a paper-based substrate with desirable power output with the standard thermoelectric (TE) materials that have been previously utilized. In this direction, Cu2SnS3(CTS), an earth-abundant, ternary sulfide, can be a good choice p-type semiconductor, when paired with a suitable n-type TE material. In this article, CTS nanocubes are synthesized via a simple hot injection method and a thick film device on emery paper was prepared and optimized. Furthermore, a flexible, 20-pair PTEG is fabricated with p-type CTS legs and traced and pressed n-type bismuth legs assembled using Kapton tape that produced a significantly high output power of 2.18 μW (output power density ∼0.85 nW cm–2K–1) for a temperature gradient of ΔT= 80 K. The TE properties are also supported by finite element simulation. The bending test conducted for the PTEG suggests device stability for up to 800 cycles with <0.05% change in the internal resistance. A proof-of-concept field-based demonstration for energy harvesting from waste heat of a motorbike exhaust is shown recovering an output power of ∼42 nW for ΔT= 20 K, corroborating the experimental and theoretical results.
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- 2023
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7. Copper-Catalyzed Regioselective Remote C–H Bond Chalcogenation of Aromatic Amine Derivatives without Using Any Large Template.
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Paul, Biprajit, Das, Surajit, and Chatterjee, Indranil
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- 2023
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8. Formulation and Skin Permeation of Active-Loaded Lipid Nanoparticles: Evaluation and Screening by Synergizing Molecular Dynamics Simulations and Experiments.
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Gupta, Krishna M., Das, Surajit, Wong, Annie B. H., and Chow, Pui Shan
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- 2023
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9. Study and experimental investigation of insertion force modeling and tissue deformation phenomenon during surgical needle-soft tissue interaction
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Barua, Ranjit, Das, Surajit, Datta, Sudipto, Datta, Pallab, and Roy Chowdhury, Amit
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Surgical needle insertion is generally used in the current advanced surgery process, particularly in the area of biopsy, MIS or minimally invasive surgery, brachytherapy, etc. During the needle insertion performance into the soft tissue, one of the most important issues is the tissue deformation that affects the needle engagement inside the tissue material. We have presented an energy-based insertion model, and the conical shape of deformed tissue is assumed. The tests are performed on PVA gel samples, and the model is analyzed to determine the tissue deformation volume during needle insertion into soft tissue. The procedures for evaluating the puncture force which creates the deformation of the contact point. With the help of the needle insertion experimental investigation, a geometric model of tissue deformation phenomenon and insertion force was investigated. The active needle-soft tissue contacts are studied, and with the help of the energy-based insertion model, the different factors like strain energy, potential, and dissipated energies are investigated. The tests are completed on the tissue mimic PVA gel samples, and the results show the volume and area of tissue deformation; at the time of initial needle insertion, the maximum tissue deformation arises and during the needle movement in the post-perforation stage, the deformation gradually decreases as the more peripheral work is altered into the vicious and degenerate energies. The maximum insertion force was noticed in 14° bevel angle needle tip and at 3 mm/s needle insertion speed, the volume of tissue deformation was maximum. The maximum deformation of the issue arises at the initial perforate position, and the distortion reduces along with the raises of the needle movement during the post-insertion period as the needle insertion work is converted into vicious and dissolute energies.
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- 2023
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10. Copper-Catalyzed Regioselective Remote C–H Bond Chalcogenation of Aromatic Amine Derivatives without Using Any Large Template
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Paul, Biprajit, Das, Surajit, and Chatterjee, Indranil
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A mild and convenient strategy has been developed for the para-selective chalcogenation of anilide scaffolds via C–H bond functionalization. This methodology employs one of the most earth-abundant and inexpensive Cu(II) catalysts and a commercially available simple aryl chalcogen source without any complex directing template, exogenous ligand, acid/base, oxidant, or other additives. The key feature of this methodology is an impressive regioselectivity along with a wide range of functional group tolerance with good to excellent yields under aerobic conditions.
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- 2023
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11. Experimental and simulation investigation of surgical needle insertion into soft tissue mimic biomaterial for minimally invasive surgery (MIS)
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Barua, Ranjit, Das, Surajit, Roy Chowdhury, Amit, and Datta, Pallab
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The surgical needle insertion process is widely applied in medical interference. During the insertion process, the inhomogeneity and denseness of the soft tissues make it tough to detect the essential tissue damage, a rupture occurs that contains huge forces and material deformations. This study is very important, as all the above-mentioned factors are very significant for modern invasive surgery so that the success rate of the surgery can increase and the patient recovers smoothly. This investigation intends to perform minimally invasive surgical (MIS) procedures and reduce the living tissue damage while performing the biopsy, PCNL, etc. A fracture mechanics method was analyzed to create a needle insertion model which can estimate the needle insertion force during inset in tissue-like PVA gel. The force model was calculated by needle insertion experimentally, and also estimated the needle tip geometry, and diameter influences the fracture toughness. Validate exp. results with simulation results and other papers. It is observed that needle diameter has a significant effect on fracture toughness, whereas the insertion velocity has a slight impact on the fracture toughness. During the rotational needle insertion process, the winds-up of the gel occurs and the diameter of the hole was increasing with increased rpm. Maximum insertion force was noticed in the 27 G needle at 5 mm/s. The interaction function will be less at the maximum fracture development region.
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- 2023
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12. Formulation and Skin Permeation of Active-Loaded Lipid Nanoparticles: Evaluation and Screening by Synergizing Molecular Dynamics Simulations and Experiments
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Gupta, Krishna M., Das, Surajit, Wong, Annie B. H., and Chow, Pui Shan
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Encapsulation into nanoparticles (NPs) is a potential method to deliver pharmaceutical/cosmetic actives deep into the skin. However, understanding the NP formulations and underlying mechanism of active delivery to skin has scarcely been studied. We report a simulation platformthat screens, evaluates, formulates, and provides atomic-resolution interpretation of NP-based formulations, and reveals the active permeation mechanism from NPs to skin. First, three actives, namely, ferulic acid (FA), clotrimazole (CZE), and tretinoin (TTN), and five lipid excipients’ (Compritol, Precirol, Geleol, Gelot, Gelucire) combinations were screened by MD simulations for the best pairs. For each suggested pair, the actual active and lipid compositions for the synthesis of stable NP formulations were then obtained by experiments. MD simulations demonstrate that in NP formulations, FA and CZE actives are present at the surface of the NPs, whereas TTN actives are present at both the surface and interior of the NP core. The NP shapes obtained by simulation perfectly match with experiments. For each NP, separate MD simulations illustrate that active-loaded NPs approach the skin surface quickly, and then actives translocate from NP surface to skin surface followed by penetration of NPs through skin. The driving force for the translocation which initiates during the penetration process, is the stronger active-skin interaction compared to active-NP interaction. Permeation free energy indicates spontaneous transfer of actives from solution phase to the surface of the skin bilayer. The free energy barriers are increased in the order of FA < TTN < CZE. Significantly lower diffusions of actives are obtained in the main barrier region compared to bulk, and the average diffusion coefficients of actives are in the same order of magnitude (∼10–6cm2/s). The estimated permeability coefficients (log P) of actives are mainly governed by free energy barriers. The study would facilitate the development of novel lipid-based NP formulations for personal-care/pharmaceutical applications.
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- 2023
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13. Simulation and experimental investigation of the surgical needle deflection model during the rotational and steady insertion process
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Barua, Ranjit, Das, Surajit, RoyChowdhury, Amit, and Datta, Pallab
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Needle insertion is executed in numerous medical and brachytherapy events. Exact needle insertion into inhomogeneous soft biological tissue is of useful importance due to its significance in clinical diagnosis (especially percutaneous) and treatments. The surgical needles used in such processes can deflect during the percutaneous process. Needle deflecting which affects needle — soft tissue interface and needle controllability have a crucial role in establishment precision. In this paper, we have analyzed a mechanics-based model both rotational and non-rotational needle insertion, and studied the deflection phenomenon in both insertion cases, we validated it with a real-time nonlinear Dassault Systèmes®ABAQUS simulation model. For definite contact force, the maximum the contact stiffness was, the minimum it inserted, the cohesive surface model was used to investigate the needle insertion analysis, where the fracture point was defined by a failure strain and with the help of the in, the fully failed components would be removed. Using living tissue comparable PVA gel materials, the needle insertion force model is developed from insertion experimentations with the help of two different processes (rotational and non-rotational needle insertion). In a rotational needle, deflection is less than in a non-rotational needle. The preliminary insertion was observed in the rotational needle at 1.261 mm (experiment), and 1.538 mm (simulation), and for non-rotational needle insertion, the initial insertion was noticed at 1.756 mm (experiment) and 1.982 mm (simulation). The main aim of this study is to navigate the surgical needle in an accurate way to reduce the erroneousness for a clinical diagnosis like anesthesia, brachytherapy, biopsy, and modern microsurgery operation.
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- 2023
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14. Chapter Three - Microbial community signatures for estimation of postmortem time intervals.
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Dash, Hirak Ranjan and Das, Surajit
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The human body provides a complex ecosystem for symbiotic habitation of a huge number of microorganisms. These commensal microorganisms provide a huge benefit to the living host by acting against many deadly infections. Once the host dies, many changes in the complex ecosystem of the human body take place. The personalized microbes of a human body undergo successional change as many exogenous microbes attack the nutrient-rich cadaver after death. The succession pattern change of microbes in human cadaver allows postulating different models for estimation of Postmortem time interval (PMI). Estimation of PMI has a broad prospect from the criminal investigation point of view. Though many techniques are being used nowadays to estimate PMI, all of them have their pros and cons. With the advent of advanced molecular biological techniques, studies on the thanatomicrobiome of a human cadaver have gained pace and provide a superior alternative for conventional methods of PMI estimation. This chapter summarizes the recent advancements in the changes in signature microflora postmortem with change in human microenvironment to postulate a consensus model for estimation of PMI. [ABSTRACT FROM AUTHOR]
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- 2022
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15. FedEL: Federated Education Learning for generating correlations between course outcomes and program outcomes for Internet of Education Things
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Sengupta, Diganta, Khan, Soumya Suvra, Das, Surajit, and De, Debashis
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In this study, Federated Learning (FL) has been used for decision making on decentralized Internet of Things (IoT) devices which store private educational data. The decentralized IoT devices form the edge devices at the institution/client level, i.e. each edge device is a single institution; and the decision making is done at the server (university) level. We propose a social Internet of Education Things framework which generates the correlations between the Course Outcomes (CO) and the Program Outcomes (PO) at the client for a certain course. The correlations are then aggregated at the server using fed-averaging. The CO-PO correlations at the edge are generated using distance metric between the semantics of the CO, the PO, and the syllabus. The CO-PO correlations are generated at the client in two ways — manually as well as using an AI model based on semantic analysis. The deviations between the manual and the AI model output is noted and sent to the server which aggregates the deviations, and feeds them back to the AI models at the clients. The clients adjust their CO-PO correlations based on the aggregated deviation values, and generate final CO-PO correlations based on the threshold values of [0, 1, 2, 3] corresponding to [No Correlation, Low, Medium, High] correlations. We observe a performance accuracy of 83.33% and a loss of 0.51 for our framework. To the entirety of our understanding, this study is the first attempt to use FL for generating CO-PO correlations at the edge/institution level.
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- 2024
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16. Reconstruction of [formula omitted] gravity model via the Raychaudhuri equation.
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Panda, Arijit, Das, Surajit, Manna, Goutam, and Ray, Saibal
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In this work, we investigate for an analytical solution under modified gravity theory, specifically the f (R , T) gravity for two different eras, i.e., matter and dark energy dominated accelerating universe from completely geometrical and mathematical point of view with the help of the Raychaudhuri equation. To construct f (R , T) gravity model, we consider the functional form of f (R , T) as the sum of two independent functions of the Ricci scalar R and the trace of the energy–momentum tensor T , respectively. Under the consideration of this type of power law expansion of the Universe we have studied the viability, stability and all the energy conditions. We note that the strong energy condition is not satisfied in our model, which is obvious for the present scenario of the Universe. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Anticancer activity of Bacopa monnieri through apoptosis induction and mitophagy-dependent NLRP3 inflammasome inhibition in oral squamous cell carcinoma.
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Mishra, Soumya Ranjan, Behera, Bishnu Prasad, Singh, Vineet Kumar, Mahapatra, Kewal Kumar, Mundkinajeddu, Deepak, Bhat, Deeksha, Minz, Aruna Mukti, Sethi, Gautam, Efferth, Thomas, Das, Surajit, and Bhutia, Sujit Kumar
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• Aqueous fraction of ethanolic extract of Bacopa monnieri (BM-AF) was found to have potent anticancer potential against oral cancer cells. • BM-AF induced PARKIN dependent mitophagy in oral cancer cells. • BM-AF inhibited arecoline-induced NLRP3 inflammasome activation in both in-vitro and in-vivo oral cancer model. • BM-AF mediated NLRP3 inflammasome inhibition was mitophagy dependent. Bacopa monnieri (BM) is traditionally used in human diseases for its antioxidant, anti-inflammatory and neuroprotective effects. However, its anticancer potential has been poorly understood. The aim of this study was to explore the detailed anticancer mechanism of BM against oral cancer and to identify the bioactive BM fraction for possible cancer therapeutics. We performed bioactivity-guided fractionation and identified that the aqueous fraction of the ethanolic extract of BM (BM-AF) had a potent anticancer potential in both in vitro and in vivo oral cancer models. BM-AF inhibited cell viability, colony formation, cell migration and induced apoptotic cell death in Cal33 and FaDu cells. BM-AF at low doses promoted mitophagy and BM-AF mediated mitophagy was PARKIN dependent. In addition, BM-AF inhibited arecoline induced reactive oxygen species production in Cal33 cells. Moreover, BM-AF supressed arecoline-induced NLR family pyrin domain containing 3 (NLRP3) inflammasome activation through mitophagy in Cal33 cells. The in vivo antitumor effect of BM-AF was further validated in C57BL/6J mice through a 4-nitroquinolin-1-oxide and arecoline-induced oral cancer model. The tumor incidence was significantly reduced in the BM-AF treated group. Further, data obtained from western blot and immunohistochemistry analysis showed increased expression of apoptotic markers and decreased expression of inflammasome markers in the tongue tissue obtained from BM-AF treated mice in comparison with the non-treated tumor bearing mice. In conclusion, BM-AF exhibited potent anticancer activity through apoptosis induction and mitophagy-dependent inhibition of NLRP3 inflammasome activation in both in vitro and in vivo oral cancer models. Moreover, we have investigated apoptosis and mitophagy-inducing compounds from this plant extract having anticancer activity against oral cancer cells. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Damage detection in a cantilever beam using noisy mode shapes with an application of artificial neural network-based improved mode shape curvature technique
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Gupta, Sonu Kumar and Das, Surajit
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Experimentally and numerically obtained displacement mode shapes are utilized as input data to artificial neural networks (ANNs) and mode shape curvature technique. Frequency responses (FRs) in the form of displacement mode shapes with varying damage levels are extracted using the Bruel & Kjaer instrument. Two identical specimens of a cantilever beam are considered with different damage locations. It is demonstrated that the measured frequency response needs to be made error-free to locate damages. ANN training algorithms are utilized to reduce the measurement error from the measured frequency response (FR) data set. The analysis is more robust due to the use of ANN application before extracting mode shape curvature. The trained data sets are then utilized to produce the mode shapes curvatures for all the damage cases using central difference approximation. Damage severity and locations are then identified by analyzing the absolute mode shape curvature differences in different damage scenarios.
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- 2021
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19. Expediency of Tetra- and Pentanucleotide Repeat Autosomal STR Markers for DNA Typing in Central Indian Population.
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Dash, Hirak R., Shrivastava, Pankaj, and Das, Surajit
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- 2020
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20. Oxidative stress-induced DNA damage and DNA repair mechanisms in mangrove bacteria exposed to climatic and heavy metal stressors.
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Rath, Sonalin and Das, Surajit
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DNA repair ,DNA damage ,HEAVY metals ,MANGROVE plants ,GLUTATHIONE transferase ,LEAD - Abstract
Bacteria thriving in the mangrove ecosystem are major drivers of elemental cycles. Climate change and environmental stressors (heavy metals) influence the performance of these microorganisms, thereby affecting the biogeochemical cycle. The present study reports the genotoxic effect of climatic and heavy metal stressors on mangrove bacteria and their adaptation strategies. Comparative analysis between two bacterial strains, Bacillus stercoris GST-03 and Pseudomonas balearica DST-02 isolated from the Bhitarkanika mangrove ecosystem, Odisha, India, showed cellular injuries in response to various stressors as evident by declined growth, elevated levels of reactive oxygen species (ROS) and resulted DNA damage. B. stercoris GST-03 showed more tolerance towards acidic pH, whereas P. balearica DST-02 showed higher tolerance towards UV exposure and heavy metals (Lead and Cadmium). The adaptation strategies of the strains revealed a significant role of GST in ROS scavenging activity and the involvement of Nucleotide excision repair or SOS response pathways. However, irreparable DNA damage was observed at pH 9 and 200 ppm Cd in B. stercoris GST-03, and at pH 4, 1000 ppm of Pb and 200 ppm of Cd in P. balearica DST-02. The current findings provide a broad overview of bacterial response and adaptability concerning future climate and environmental changes. [Display omitted] • Climatic stressors and heavy metals influence the performance of mangrove bacteria. • Resilience of B. stercoris GST-03 towards future ocean acidification was observed. • P. balearica DST-02 found to tolerate higher UV exposure and heavy metals concentration. • Glutathione S transferase is indispensable for ROS scavenging in mangrove bacteria. • Irreparable DNA damage occurs at lower pH and high heavy metal concentrations. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Leading-order corrections to the thermodynamics of Rindler modified Schwarzschild black hole.
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Mandal, Surajit, Das, Surajit, Gogoi, Dhruba Jyoti, and Pramanik, Ananda
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In this work, we present a thermodynamical study of a Rindler modified Schwarzschild black hole under the consideration of small thermal fluctuations. In particular, we compute various stable macroscopic thermodynamic variables such as Hawking temperature, entropy, Helmholtz free energy, internal energy, enthalpy, and Gibbs free energy. To explore the effects of small statistical thermal fluctuations on stable thermodynamical parameters, we estimated the corrections to the various thermodynamical potentials of Rindler modified Schwarzschild black hole up to the first (leading) order and do a comparative study for the different values of correction parameter and Rindler acceleration parameter for fixed values of a cosmological constant. In this study, we examine the stability of black holes in the presence of thermal fluctuations. We find that when the correction parameter is positive, small-sized black holes remain stable, while large-sized ones become unstable. Conversely, when the correction parameter is negative, both small and large black holes exhibit instability. Additionally, we demonstrate that the first law of thermodynamics remains valid even in the presence of thermal fluctuations. [ABSTRACT FROM AUTHOR]
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- 2023
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22. Green Synthesis of Iron Oxide Nanoparticles Mediated by Filamentous Fungi Isolated from Sundarban Mangrove Ecosystem, India
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Mahanty, Shouvik, Bakshi, Madhurima, Ghosh, Somdeep, Chatterjee, Shreosi, Bhattacharyya, Subarna, Das, Papita, Das, Surajit, and Chaudhuri, Punarbasu
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In the present study, biosynthesis of iron oxide nanoparticles (IONPs) was achieved using three manglicolous fungi, STSP10 (Trichoderma asperellum), STSP 19 (Phialemoniopsis ocularis) and STSP 27 (Fusarium incarnatum) isolated from estuarine mangrove sediment of Indian Sundarban. Synthesised IONPs were initially monitored by UV-Vis spectrophotometer and further characterised by Fourier transform infrared (FTIR) spectroscopy, which provides information regarding proteins and other organic residues involved with iron nanoparticle. The morphology of iron nanoparticle were found to be spherical with average particle size ranging between 25 ± 3.94 nm for T. asperellum, 13.13 ± 4.32 nm for P. ocularisand 30.56 ± 8.68 nm for F. incarnatum, which were confirmed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). Energy-dispersive x-ray analysis (EDX) analysis was performed during FESEM study to confirm the presence of elemental Fe in the sample. X-ray diffraction (XRD) pattern has shown that the IONPs are iron oxide in nature.
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- 2019
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23. SIMAS: smart IoT model for acute stroke avoidance
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Nayak, Samaleswari Prasad, Das, Surajit, Rai, S.C., and Pradhan, Sateesh Kumar
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There is an increase of chronic heart diseases, which require periodic monitoring and anytime healthcare nursing facility to a patient. Due to several constraints, availing physical medical healthcare is a nightmare for some patients. With the advancement in medical sciences and Internet of Things (IoT), the status of a patient can be monitored ubiquitously. As a primary diagnostic assessment, correct heart rate and body temperature are the two necessary parameters to be measured first. Considering the scenario we have developed an IoT based healthcare model SIMAS, to record both parameters efficiently and with desired accuracy. It would be suitable for the persons undergoing intense physical activities - especially sports persons. The collection of real-time data can be carried out by the body sensors attached to the person and can also be monitored remotely. In addition the online monitoring process ensures prevention of any mishaps at any event places.
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- 2019
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24. Optimizing Output Performances in Stationery Papers–Based Hybrid Inorganic–Organic Flexible Thermoelectric Generators
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Mondal, Bhargab P., Das, Surajit, Ranjan, Priya, and Datta, Anuja
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Flexible and foldable paper‐based thermoelectric generators (PTEGs) have drawn industrial attention due to the wide applications in heat energy harvesting and sensing. Herein, optimization of the output performances of flexible and hybrid inorganic–organic PTEGs fabricated on stationery paper substrates from poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) and graphite as p‐type and n‐type materials, respectively, is presented. By choosing simplistic processes such as polyethyleneimine (PEI)‐treated graphite pencil traces and brush‐painted PEDOT:PSS films, robust and sustainable PTEG devices are fabricated. It is first time shown that different qualities of stationery papers can have significant impact on the output performance of PTEGs, attributed to their variance in substrate roughness. Thus, output powers of ≈1.93 and ≈0.68 nW for ΔT= 70 K are obtained for TE generators prepared from emery and office paper legs (four‐pair assembled on Kapton), respectively, suggesting emery paper to have significant better performance. Transient flexibility and fatigue of each device type are also tested where emery paper–based PTEG appears to be more robust. A detail comparison of the device performances on the different types of paper substrates are exclusively presented experimentally and thereafter computationally validated by COMSOL modeling to predictably control and enhance the output performance of reported PTEGs. Herein, output performances of paper‐based thermoelectric generators (PTEGs) are studied as function of substrate roughness of different grades of stationery papers. PTEG is fabricated using p‐type poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate and n‐type graphite. Emery paper‐based device shows manifold performance enhancement (1.93 nW) as compared to office paper‐based device (0.68 nW) at ΔT= 70 K, also validated by COMSOL.
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- 2023
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25. Lrcasp9 shares similarity in structural motifs with human caspase-9 and is activated following bacterial infection and anti-viral vaccination
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Giri, Alok, Paichha, Mahismita, Saha, Ashis, Das, Surajit, and Samanta, Mrinal
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Among various caspases, caspase-9 plays a crucial role in the initiation phase of apoptotic cascade. To investigate about it in a high-valued freshwater fish species rohu (Labeo rohita), we cloned and characterized full-length caspase-9 cDNA (Lrcasp9) and analyzed its expression following bacterial infections and anti-viral vaccinations. The Lrcasp9 consisted of 1619-bp nucleotides (nt) having an ORF of 1302 nt encoding a polypeptide of 433 amino acids (aa) with a molecular mass of ∼ 48.20 kDa. Structurally, Lrcasp9 comprised of one CARD domain (1–89 aa) and one CASc domain (161–430 aa). The CASc domain consisted of one large subunit (p20) spanning from 168 to 300 aa, and a small sub unit (p10) from 343 to 430 aa. The caspase family signature histidine active motif H233SAYDCCVVIILSHG247, cysteine active motif K287PKLFFIQACGG298and pentapeptide “QACGG” active sites present in the p20 domain of Lrcasp9 was conserved across fish species, mouse and human caspase-9. Phylogenetically, it was closely related to common carp caspase-9 and exhibited significant similarity (90.1%) and identity (85.3%) in their amino acid sequence. In the uninfected fish, Lrcasp9 gene expression was highest (~ 5.3-fold) in blood and lowest in gill. In response to Aeromonas hydrophilaand Edwardsiella tardainfection and rhabdoviral vaccination, Lrcasp9 gene expression was significantly (p> 0.05) enhanced in gill, liver, kidney and spleen, and also in vitro during cell death, suggesting activation of the intrinsic apoptotic pathway in bacterial infections and anti-viral vaccination in Labeo rohita.
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- 2018
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26. Prospects of biosynthesized nanomaterials for the remediation of organic and inorganic environmental contaminants
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Das, Surajit, Chakraborty, Jaya, Chatterjee, Shreosi, and Kumar, Himanshu
- Abstract
Nanotechnology deals with matter at dimensions within 1–100 nm, with such material displaying a wide range of applications in industry, medicine and environmental remediation. Futuristic approaches for the green synthesis of nanomaterials (NMs) and nanoparticles (NPs) viamodification to control their size and shape can enhance their qualities for future applications. The new horizon of opportunities and potential for innovation together with the impact of recent advances in nanotechnology research have led to the recognition of these materials for application in environmental remediation. Diverse microorganisms, microbially synthesized compounds and plant extracts-mediated biosynthesized NMs/NPs have significant relevance in remediation. Biological nanofactories yield NPs that are considered more cost-effective and environmentally friendly than conventional chemical methods. Nanoremediation incorporates NMs, NPs, nanomembranes and nanopowders for detection as well as for the removal of toxic metals and organic compounds from the environment. Various processes of adsorption, transformation, photocatalysis and catalytic reduction have been achieved by various inorganic and organic nanoscale materials for the removal of toxic metals and organic compounds. Nanobiosensors have also been utilized to detect heavy metals and persistent organic pollutants and could be commercialized for their application in environment sampling. This review summarizes the different types of biologically synthesized NMs/NPs together with their mechanisms for the biosensing and remediation of environmental pollutants.
- Published
- 2018
- Full Text
- View/download PDF
27. Bacillusand biopolymer: Prospects and challenges
- Author
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Mohapatra, Swati, Maity, Sudipta, Dash, Hirak Ranjan, Das, Surajit, Pattnaik, Swati, Rath, Chandi Charan, and Samantaray, Deviprasad
- Abstract
The microbially derived polyhydroxyalkanoates biopolymers could impact the global climate scenario by replacing the conventional non-degradable, petrochemical-based polymer. The biogenesis, characterization and properties of PHAs by Bacillusspecies using renewable substrates have been elaborated by many for their wide applications. On the other hand Bacillusspecies are advantageous over other bacteria due to their abundance even in extreme ecological conditions, higher growth rates even on cheap substrates, higher PHAs production ability, and the ease of extracting the PHAs. Bacillusspecies possess hydrolytic enzymes that can be exploited for economical PHAs production. This review summarizes the recent trends in both non-growth and growth associated PHAs production by Bacillusspecies which may provide direction leading to future research towards this growing quest for biodegradable plastics, one more critical step ahead towards sustainable development.
- Published
- 2017
- Full Text
- View/download PDF
28. Bacterial biofilms and quorum sensing: fidelity in bioremediation technology.
- Author
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Mangwani, Neelam, Kumari, Supriya, and Das, Surajit
- Abstract
Increased contamination of the environment with toxic pollutants has paved the way for efficient strategies which can be implemented for environmental restoration. The major problem with conventional methods used for cleaning of pollutants is inefficiency and high economic costs. Bioremediation is a growing technology having advanced potential of cleaning pollutants. Biofilm formed by various micro-organisms potentially provide a suitable microenvironment for efficient bioremediation processes. High cell density and stress resistance properties of the biofilm environment provide opportunities for efficient metabolism of number of hydrophobic and toxic compounds. Bacterial biofilm formation is often regulated by quorum sensing (QS) which is a population density-based cell–cell communication process via signaling molecules. Numerous signaling molecules such as acyl homoserine lactones, peptides, autoinducer-2, diffusion signaling factors, and α-hydroxyketones have been studied in bacteria. Genetic alteration of QS machinery can be useful to modulate vital characters valuable for environmental applications such as biofilm formation, biosurfactant production, exopolysaccharide synthesis, horizontal gene transfer, catabolic gene expression, motility, and chemotaxis. These qualities are imperative for bacteria during degradation or detoxification of any pollutant. QS signals can be used for the fabrication of engineered biofilms with enhanced degradation kinetics. This review discusses the connection between QS and biofilm formation by bacteria in relation to bioremediation technology. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
29. Marine Bacterial Biofilms in Bioremediation of Polycyclic Aromatic Hydrocarbons (PAHs) Under Terrestrial Condition in a Soil Microcosm
- Author
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MANGWANI, Neelam, KUMARI, Supriya, and DAS, Surajit
- Abstract
Polycyclic aromatic hydrocarbons (PAHs) in soil retain for a quite long period due to their hydrophobicity and aggregation properties. Biofilm-forming marine bacterial consortium (named as NCPR), composed of Stenotrophomonas acidaminiphilaNCW702, Alcaligenes faecalisNCW402, Pseudomonas mendocinaNR802, Pseudomonas aeruginosaN6P6, and Pseudomonas pseudoalcaligenesNP103, was used for the bioremediation of PAHs in a soil microcosm. Phenanthrene and pyrene were used as reference PAHs. Parameters that can affect PAH degradation, such as chemotaxis, solubility of PAHs in extracellular polymeric substances (EPS), and catechol 2,3-dioxygenase (C23O) activity, were evaluated. P. aeruginosaN6P6 and P. pseudoalcaligenesNP103 showed chemotactic movement towards both the reference PAHs. The solubility of both the PAHs was increased with an increase in EPS concentration (extracted from all the 5 selected isolates). Significantly (P <0.001) high phenanthrene (70.29%) and pyrene (55.54%) degradation was observed in the bioaugmented soil microcosm. The C23O enzyme activity was significantly (P < 0.05) higher in the bioaugmented soil microcosm with phenanthrene added at 173.26 ± 2.06 nmol min−1mg−1protein than with pyrene added at 61.80 ± 2.20 nmol min−1mg−1protein. The C23O activity and gas chromatography-mass spectrometer analyses indicated catechol pathway of phenanthrene metabolism. However, the metabolites obtained from the soil microcosm added with pyrene revealed both the catechol and phthalate pathways for pyrene degradation.
- Published
- 2017
- Full Text
- View/download PDF
30. Seasonal prevalence and blood meal analysis of filarial vector Culex quinquefasciatus in coastal areas of Digha, West Bengal, India.
- Author
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Azmi, Syed Afrin, Das, Surajit, and Chatterjee, Soumendranath
- Published
- 2015
31. On the Vehicle Routing Problem.
- Author
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Dehne, Frank, López-Ortiz, Alejandro, Sack, Jörg-Rüdiger, Berman, Piotr, and Das, Surajit K.
- Abstract
In the Vehicle Routing Problem (VRP), as in the Traveling Salesman Problem (TSP), we have a metric space of customer points, and we have to visits all customers. Additionally, every customer has a demand, a quantity of a commodity that has to be delivered in our vehicle from a single point called the depot. Because the vehicle capacity is bounded, we need to return to the depot each time we run out of the commodity to distribute. We describe a fully polynomial time algorithm with approximation 2.5, we also modify this algorithm for the on-line version of VRP, the randomized version has competitive ratio of 2.5 on the average, and the deterministic version has ratio 4. We also describe 2 approximation for a restricted version of the problem. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
32. Synergistic effect of quorum sensing genes in biofilm development and PAHs degradation by a marine bacterium
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Kumari, Supriya, Mangwani, Neelam, and Das, Surajit
- Abstract
ABSTRACTQuorum sensing (QS) is a prevalently found intercellular signaling system in bacteria. QS system bestows behavioral coordination ability in bacteria at high population density. QS via acylated homoserine lactone (AHL) is extensively conserved in Gram-negative bacteria and plays crucial role in regulating many biological processes. The role of QS genes coding for AHL synthase enzyme (lasI and rhlI) was established in bioremediation of polycyclic aromatic hydrocarbons (PAHs) viz. phenanthrene and pyrene. AHL producing biofilm forming marine bacterium Pseudomonas aeruginosaN6P6 was isolated by selective enrichment on PAHs. AHL production was confirmed using AHL bioreporters and GC-MS analysis. Biofilm development and its architecture was significantly (P < 0.05) affected by alterations in lasI/rhlI expression. The lasI/rhlI gene expression pattern significantly influences biofilm formation and subsequent degradation of PAHs. The integrated density of Pseudomonas aeruginosaN6P6 biofilm was highest for 48 h old biofilm and the PAHs (phenanthrene and pyrene) degradation was also found maximum (85.6 % and 47.56 %) with this biofilm. A significant positive correlation (P< 0.05) was observed between lasIexpression and PAHs degradation. The role of QS genes in biofilm formation and degradation of PAHs was validated by blocking the transcription of lasI/rhlI by a QS inhibitor (QSI) tannic acid. Further, application of such QS positive isolates in PAHs contaminated sites could be a promising strategy to improve the PAHs bioremediation.
- Published
- 2016
- Full Text
- View/download PDF
33. Prospects of Bacteriotherapy with Nanotechnology in Nanoparticledrug Conjugation Approach for Cancer Therapy
- Author
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Raj, Ritu and Das, Surajit
- Abstract
Bacteriotherapy and nanotechnology have shown remarkable potential in diagnostic and therapeutic applications for various diseases. Individual impacts of these micro-nano systems over different aspects of human health are well studied; however, an integrated system of bacteria-nanoparticle (NP) conjugation is less explored. The untamed potential of bacteria-NP conjugation could be a new tool for diagnosis and treatment of invasive diseases like malaria, tuberculosis and cancer. Mammalian cells exhibit cytosis as their defense mechanism when they encounter foreign elements such as bacteria. In these mammalian cells, during phagocytosis, bacteria are ruptured and lysed by lysozymes. A bacterium carrying the drug-tagged NP would be engulfed in the same manner and ultimately reaches the target cells. Rapid and continuous cell divisions in the cancer tissues lead to defective vessels, underdeveloped cellcell interconnects, development of hypoxic areas and heterogeneous population of tumor cells. This unorganized and poorly developed angiogenesis in tumor cells makes it difficult for conventional chemotherapeutic drugs to localize the tumors selectively. In the present scenario of diagnosis and treatment of cancer/tumor cells, it could be expected that the existing bacteriotherapy with the advanced nanotechnology would be a way further in the targeted drug delivery for cancer therapy. This review emphasizes the potential applications of bacteriotherapy with nanotechnology for the diagnosis and treatment of cancer.
- Published
- 2016
34. Bacterial biofilms and quorum sensing: fidelity in bioremediation technology
- Author
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Mangwani, Neelam, Kumari, Supriya, and Das, Surajit
- Abstract
Increased contamination of the environment with toxic pollutants has paved the way for efficient strategies which can be implemented for environmental restoration. The major problem with conventional methods used for cleaning of pollutants is inefficiency and high economic costs. Bioremediation is a growing technology having advanced potential of cleaning pollutants. Biofilm formed by various micro-organisms potentially provide a suitable microenvironment for efficient bioremediation processes. High cell density and stress resistance properties of the biofilm environment provide opportunities for efficient metabolism of number of hydrophobic and toxic compounds. Bacterial biofilm formation is often regulated by quorum sensing (QS) which is a population density-based cell–cell communication process via signaling molecules. Numerous signaling molecules such as acyl homoserine lactones, peptides, autoinducer-2, diffusion signaling factors, and α-hydroxyketones have been studied in bacteria. Genetic alteration of QS machinery can be useful to modulate vital characters valuable for environmental applications such as biofilm formation, biosurfactant production, exopolysaccharide synthesis, horizontal gene transfer, catabolic gene expression, motility, and chemotaxis. These qualities are imperative for bacteria during degradation or detoxification of any pollutant. QS signals can be used for the fabrication of engineered biofilms with enhanced degradation kinetics. This review discusses the connection between QS and biofilm formation by bacteria in relation to bioremediation technology.
- Published
- 2016
- Full Text
- View/download PDF
35. Ocean acidification and marine microorganisms: responses and consequences
- Author
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Das, Surajit and Mangwani, Neelam
- Abstract
Ocean acidification (OA) is one of the global issues caused by rising atmospheric CO2. The rising pCO2and resulting pH decrease has altered ocean carbonate chemistry. Microbes are key components of marine environments involved in nutrient cycles and carbon flow in marine ecosystems. However, these marine microbes and the microbial processes are sensitive to ocean pH shift. Thus, OA affects the microbial diversity, primary productivity and trace gases emission in oceans. Apart from that, it can also manipulate the microbial activities such as quorum sensing, extracellular enzyme activity and nitrogen cycling. Short-term laboratory experiments, mesocosm studies and changing marine diversity scenarios have illustrated undesirable effects of OA on marine microorganisms and ecosystems. However, from the microbial perspective, the current understanding on effect of OA is based mainly on limited experimental studies. It is challenging to predict response of marine microbes based on such experiments for this complex process. To study the response of marine microbes towards OA, multiple approaches should be implemented by using functional genomics, new generation microscopy, small-scale interaction among organisms and/or between organic matter and organisms. This review focuses on the response of marine microorganisms to OA and the experimental approaches to investigate the effect of changing ocean carbonate chemistry on microbial mediated processes.
- Published
- 2015
- Full Text
- View/download PDF
36. Mercury Resistant Bacterial Population and Characterization of Bacillus sp., Isolated From Sediment of Solid Waste Discharged Point of Steel Industry.
- Author
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Giri, Sindhushree, Dash, Hirak, and Das, Surajit
- Published
- 2014
- Full Text
- View/download PDF
37. Ag nanoparticles immobilized over highly porous crystalline organosilica for epoxidation of styrene using CO2 as oxidant.
- Author
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Chatterjee, Sauvik, Das, Surajit, Bhanja, Piyali, E. S., Erakulan, Thapa, Ranjit, Ruidas, Santu, Chongdar, Sayantan, Ray, Suman, and Bhaumik, Asim
- Subjects
POROUS materials ,EPOXIDATION ,OXIDIZING agents ,CARBON dioxide ,STYRENE ,MESOPOROUS silica - Abstract
We report a new crystalline porous organosilica material, which stabilized tiny AgNPs to obtain a robust nanocatalyst Ag@TSOS-1 that efficiently catalyses the complete conversion of styrene into predominantly styrene oxide using CO 2 as an oxidant in a high pressure autoclave reactor. [Display omitted] • A new crystalline porous organosilica material has been synthesized using a tailor made bridging organoslilane precursor. • Ag nanoparticles were supported over porous organosilica material to design a novel nanocatalyst Ag@TSOS-1. • Ag@TSOS-1 catalyses epoxidation of styrene to styrene oxide using CO 2 as soft oxidant. • At 20 bar CO 2 pressure and 60 °C temperature only styrene oxide and polystyrene formed from styrene. Selective epoxidation of olefins is a very important reaction as epoxides are widely been used as platform chemical in polymer and pharmaceutical industries. Unlike conventional oxidants like molecular O 2 or peroxides, the use of CO 2 as a soft oxidant for the oxidation of olefins is very challenging as it offers the utilization of waste and plentiful CO 2 together with the potential for the mitigation of its harmful environmental effect. Thus, this process is cost effective and environmentally challenging. Herein, we report the synthesis of a new crystalline porous organosilica material TSOS-1 with orthorhombic crystal structure by using a tailor made bridging organoslilane precursor prepared through the Schiff base condensation of p -terphenyl-4,4′'-dialdehyde and 3-aminopropyl-trimethoxysilane. This novel crystalline material TSOS-1 has been synthesized hydrothermally in the absence of any structure-directing agent and it showed high BET surface area (220 m
2 g−1 ) and nanoscale porosity. TSOS-1 is used as a support for stabilizing tiny AgNPs to obtain a robust nanocatalyst Ag@TSOS-1, which efficiently catalyses the conversion of styrene into predominantly styrene oxide (SO) using CO 2 as a soft oxidant in an autoclave reactor under mild reaction conditions. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
38. Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillusspp.
- Author
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Bhagowati, Pabitra, Pradhan, Shreema, Dash, Hirak R., and Das, Surajit
- Abstract
Poly-β-hydroxybutyrate (PHB) is the intracellular lipid reserve accumulated by many bacteria. The most potent terrestrial bacterium Bacillus cereusSE-1 showed more PHB accumulating cells (22.1 and 40% after 48 and 72 h) than that of the marine Bacillussp. CS-605 (5 and 33% after 48 and 72 h). Both the isolates harbored phbBgene and the characteristics C=O peak was observed in the extracted PHB by Fourier transformed infrared spectroscopy analysis. Maltose was found to be the most suitable carbon source for the accumulation of PHB in B. cereusSE-1. The extracted PHB sample from B. cereusSE-1 was blended with a thermoplastic starch (TS) and an increased thermoplasticity and decreased crystallinity were observed after blending in comparison to the standard PHB. The melting temperature (Tm), melting enthalpy (∆Hf), and crystallinity (Xc) of the blended PHB sample were found to be 109.4 °C, 64.58 J/g, and 44.23%, respectively.
- Published
- 2015
- Full Text
- View/download PDF
39. Chemometric study on the biochemical marker of the manglicolous fungi to illustrate its potentiality as a bio indicator for heavy metal pollution in Indian Sundarbans.
- Author
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Mahanty, Shouvik, Tudu, Praveen, Ghosh, Somdeep, Chatterjee, Shreosi, Das, Papita, Bhattacharyya, Subarna, Das, Surajit, Acharya, Krishnendu, and Chaudhuri, Punarbasu
- Subjects
BIOMARKERS ,CHEMOMETRICS ,ECOLOGICAL risk assessment ,HEAVY metal toxicology ,HEAVY metals ,RESPONSE surfaces (Statistics) ,METAL ions ,FECAL contamination - Abstract
The study represents in vitro chemometric approach for assessing the heavy metal pollution in Indian Sundarbans. Physio-chemical and elemental characterisation of the sediment samples of Indian Sundarbans had shown high enrichments of toxic metal ions. It was characterised by elevated enrichment factors (2.16–10.12), geo-accumulation indices (0.03 –1.21), contamination factors (0.7–3.43) and pollution load indices (1.0–1.25) which showed progressive sediment quality deterioration and ecotoxicological risk due to metal ions contamination. The physio-chemical parameters of the sediments were replicated and computational chemometric modeling was utilized to assess fungal metabolic growth. All the fungi isolates had shown maximum metabolic activity in high temperature, alkaline pH, and high salinity. Further, the fungal metabolic activity was assessed in different gradient of heavy metal concentration. The significant deterioration of biochemical marker with increasing concentration of heavy metal indicates the status of the microbial health due to toxic metal pollution in the mangrove habitat. • Manglicolous fungi are effective tool to assess pollution in the mangrove sediment. • Chemometric variables were kept as per indigenous mangrove sediment parameters. • Maximum enzyme activity was observed in high temperature, salinity and alkaline pH. • Toxic metal ions disrupt the enzyme activity of the manglicolous fungi. • In vitro study provides baseline dataset for predictive analysis of mangrove health. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
40. Sorbitan Monolaurate 20 as a Potential Skin Permeation Enhancer in Transdermal Patches.
- Author
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Mukherjee, Biswajit, Kanupriya, Mahapatra, Sushmita, Das, Surajit, and Patra, Balaram
- Subjects
TRANSDERMAL medication ,POVIDONE ,DICLOFENAC ,ETHYLCELLULOSE ,ELECTRON microscopy - Abstract
The objective of our present work was to prepare transdermal matrix patches containing the drug, diclofenac diethylamine with various polymeric combinations of polyvinylpyrrolidone and ethylcellulose and to study the mechanism of release of the drug from the patches and its skin permeation. Sorbitan monolaurate 20 (Span 20), a non-ionic surfactant was added to the concentrations (0.1% wt/vol), as a skin permeation enhancer to the drug, diclofenac diethylamine. In vitro skin permeation studies, with rat skin, using a modified Keshary-Chien diffusion cell, were carried out to establish the most favorable polymeric combination and the impact of Span 20 was also studied. Extensive electron microscopy studies were conducted and the underlying mechanism of drug release from the patches and its permeation through skin are discussed. Cumulative amounts of drug released, per cm² of patch after 48 hours, were found to be 2.6111 mg, 3.53 mg. 2.498 mg, 3.373 mg, 3.716 mg, and 4.983 mg, in the formulations PA-1 (PVP: EC 1:2). PA-2 (PVP: EC 1:2 with enhancer), PA-3 (PVP: EC 1:3). PA-4 (PVP: EC 1:3 with enhancer), PA-5 (PVP: EC 3:5), and PA-6 (PVP: EC 3:5 with enhancer), respectively. There was about a 29% to 30% enhancement of skin permeation of the drug using Span 20. It is concluded here that the formulation PA-3 is the slowest in terms of skin permeation of the prepared transdermal matrix patches for diclofenac diethylamine and that Span 20 may be the best choice amongst the skin permeation enhancers to permeate the drug steadily. Moreover, extensive electron microscopic studies were conducted to understand drug distribution in the patches, drug release from the patches, and the permeation of drug through skin. [ABSTRACT FROM AUTHOR]
- Published
- 2005
41. Potentials of Polymeric Nanoparticle as Drug Carrier for Cancer Therapy: With a Special Reference to Pharmacokinetic Parameters
- Author
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Mukherjee, Biswajit, Das, Surajit, Chakraborty, Samrat, Sankar Satapathy, Bhabani, Jyoti Das, Pranab, Mondal, Laboni, Mobaswar Hossain, Chowdhury, Shekhar Dey, Niladri, and Chaudhury, Anumita
- Abstract
Nanomaterials have made a significant impact on cancer therapeutics and an emergence of polymeric nanoparticle provides a unique platform for delivery of drug molecules of diverse nature. Nanoparticles can be targeted at the tumor cells due to enhanced permeability and retention effect. Moreover, nanoparticles can be grafted by various ligands on their surface to target the specific receptors overexpressed by cancer cells or angiogenic endothelial cells. These approaches ultimately result in longer circulation half-lives, improved drug pharmacokinetics, reduced side effects of therapeutically active substances and overcoming cancer chemo-resistance thereby enhancing the therapeutic efficacy of the treatment. This review article summarizes the recent efforts in cancer nanochemotherapeutics using polymeric nanoparticles with a special reference to their pharmacokinetic and biodistribution profiles, their role in reversing multidrug resistance in cancer and strategies of tumor targeting with them, along with the challenges in the field.
- Published
- 2014
42. Mercury Resistant Bacterial Population and Characterization of Bacillussp., Isolated From Sediment of Solid Waste Discharged Point of Steel Industry
- Author
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Giri, Sindhushree, Dash, Hirak, and Das, Surajit
- Abstract
Most of the soil bacteria which are continuously exposed to mercury present in soil and sediments of lakes and rivers have genetically adapted to resist its toxicity. In the present study, mercury resistant bacterial population was enumerated from sediment samples of Rourkela Steel Plant, Odisha and a highly mercury resistant strain (MIC = 100 ppm), identified as Bacillussp., was characterized by various biochemical tests. Mercury levels in the collected sediments were in the range of 0.054–0.079 ppm. Percentages of mercury resistant bacteria in sediments at four sites were 42, 26.32, 67.56, 83.93 % respectively. The highly resistant strain Bacillussp. could grow well with 7 and 8 pH, 45 °C temperature and 6 ppt NaCl concentration. MIC of Zn, Cu, Fe and Cd are 40, 40, 40 and 20 ppm respectively. The isolate Bacillussp. SD-43 which has shown greater tolerance of mercury besides volatilizing 60.06 % of mercury as HgCl2is attributed to the adaptation towards various stress conditions and may be potentially utilized in the process of bioremediation of mercury in contaminated area.
- Published
- 2014
- Full Text
- View/download PDF
43. Dietary polyphenols in chemoprevention and synergistic effect in cancer: Clinical evidences and molecular mechanisms of action.
- Author
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Patra, Srimanta, Pradhan, Biswajita, Nayak, Rabindra, Behera, Chhandashree, Das, Surajit, Patra, Samir Kumar, Efferth, Thomas, Jena, Mrutyunjay, and Bhutia, Sujit Kumar
- Abstract
Background: Epidemiological studies has revealed that a diet rich in fruits and vegetables could lower the risk of certain cancers. In this setting, natural polyphenols are potent anticancer bioactive compounds to overcome the non-target specificity, undesirable cytotoxicity and high cost of treatment cancer chemotherapy.Purpose: The review focuses on diverse classifications of the chemical diversity of dietary polyphenol and their molecular targets, modes of action, as well as preclinical and clinical applications in cancer prevention.Results: The dietary polyphenols exhibit chemo-preventive activity through modulation of apoptosis, autophagy, cell cycle progression, inflammation, invasion and metastasis. Polyphenols possess strong antioxidant activity and control multiple molecular events through activation of tumor suppressor genes and inhibition of oncogenes involved in carcinogenesis. Numerous in vitro and in vivo studies have evidenced that these dietary phytochemicals regulate critical molecular targets and pathways to limit cancer initiation and progression. Moreover, natural polyphenols act synergistically with existing clinically approved drugs. The improved anticancer activity of combinations of polyphenols and anticancer drugs represents a promising perspective for clinical applications against many human cancers.Conclusion: The anticancer properties exhibited by dietary polyphenols are mainly attributed to their anti-metastatic, anti-proliferative, anti-angiogenic, anti-inflammatory, cell cycle arrest, apoptotic and autophagic effects. Hence, regular consumption of dietary polyphenols as food or food additives or adjuvants can be a promising tactic to preclude adjournment or cancer therapy. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
44. Ag nanoparticles immobilized over highly porous crystalline organosilica for epoxidation of styrene using CO2as oxidant
- Author
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Chatterjee, Sauvik, Das, Surajit, Bhanja, Piyali, E. S., Erakulan, Thapa, Ranjit, Ruidas, Santu, Chongdar, Sayantan, Ray, Suman, and Bhaumik, Asim
- Abstract
We report a new crystalline porous organosilica material, which stabilized tiny AgNPs to obtain a robust nanocatalyst Ag@TSOS-1 that efficiently catalyses the complete conversion of styrene into predominantly styrene oxide using CO2as an oxidant in a high pressure autoclave reactor.
- Published
- 2022
- Full Text
- View/download PDF
45. Distribution and generic composition of culturable marine actinomycetes from the sediments of Indian continental slope of Bay of Bengal
- Author
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Das, Surajit, Lyla, P., and Ajmal Khan, S.
- Abstract
Abstract: Actinomycetes population from continental slope sediment of the Bay of Bengal was studied. Samples were collected during two voyages of FORV Sagar Sampada in 2004 (May–June) and 2005 (July) respectively from 11 transects (each transect had ca. 200 m, 500 m, and 1 000 m depth stations). The physicochemical parameters of overlying water, and sediment samples were also recorded. The actinomycete population ranged from 5.17 to 51.94 CFU/g dry sediment weight and 9.38 to 45.22 CFU/g dry sediment weight during the two cruises respectively. No actinomycete colony was isolated from stations in 1 000 m depth. Two-way analysis of variance showed significant variation among stations (ANOVA two-way, P<0.05), but no significance was found between the two cruises (ANOVA two-way, P<0.05). Populations in stations in 500 m depth in both cruises were higher than that of 200 m depth stations with statistically insignificant difference (ANOVA two-way, P>0.05). Three actinomycetes genera were identified. Streptomyces was found to be the dominating one in both the cruises, followed by Micromonospora, and Actinomyces. The spore of Streptomyces isolates showed the abundance in spiral spore chain. Spore surface was smooth. Multiple regression analysis revealed that the influencing physico-chemical factors were sediment pH, sediment temperature, TOC, porosity, salinity, and pressure. The media used in the present study was prepared with seawater. Thus, they may represent an autochthonous marine flora and deny the theory of land runoff carriage into the sea for adaptation to the salinity of the seawater and sediments.
- Published
- 2008
- Full Text
- View/download PDF
46. Hypoglycemic Effect of a Lentinus edodesExo-polymer Producedfrom a Submerged Mycelial Culture
- Author
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YANG, Byung-Keun, KIM, Dong-Hyun, JEONG, Sang-Chul, DAS, Surajit, CHOI, Young-Sun, SHIN, Joon-Shik, LEE, Sang-Chul, and SONG, Chi-Hyun
- Abstract
The hypoglycemic effect of an exo-polymer produced from a submerged mycelial culture of Lentinus edodeswas investigated in streptozotocin-induced diabetic rats. The administration of the exo-polymer (200mg/kg BW) reduced the plasma glucose level by as much as 21.5%, and increased plasma insulin by 22.1% as compared to the control group. It also lowered the plasma total cholesterol and triglyceride levels by 25.1 and 44.5%, respectively. Gel chromatography of the exo-polymer revealed a single peak which is likely to have been a glycoprotein with a molecular weight of 52kDa and was found to contain 83.5% carbohydrate and 16.5% protein. The Sugar and amino acid compositions of the exo-polymer were analyzed in detail.
- Published
- 2002
- Full Text
- View/download PDF
47. Biosorption and removal of toxic heavy metals by metal tolerating bacteria for bioremediation of metal contamination: A comprehensive review.
- Author
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Priyadarshanee, Monika and Das, Surajit
- Subjects
HEAVY metals ,HEAVY metal toxicology ,BIOREMEDIATION ,METALS ,WASTEWATER treatment ,BACTERIA ,SWITCHGRASS ,SORBENTS ,BIOMASS - Abstract
• Bacteria are efficient, cost-effective adsorbents for heavy metal ions. • Different structures and components of bacteria aid in the metal uptake process. • Environmental parameters influence the metal uptake process. • Various kinetics and isotherm models are used to analyze the adsorption mechanism. • Regeneration of adsorbents is useful for several cycles of biosorption of metals. Heavy metal pollution caused due to the industrialization has been considered as a significant public health hazard, and these heavy metals exhibit various types of toxicological manifestations. Conventional remediation methods are expensive and also yield toxic by-products, which negatively affect the environment. Hence, a green technology employing various biological agents, predominantly bacteria, algae, yeasts, and fungi, has received more attention for heavy metal removal and recovery because of their high removal efficiency, low cost, and availability. However, bacterial biosorption is the safest treatment method for the toxic pollutants that are not readily biodegradable such as heavy metals. Metal biosorption by bacteria has received significant attention due to a safe, productive, and feasible technology for the heavy metal-containing wastewater treatment. These metal tolerating bacteria can bind the cationic toxic heavy metals with the negatively charged bacterial structures and live or dead biomass components. Due to the large surface area to volume ratio, these bacterial biomasses efficiently act as the biosorbent for metal bioremediation under multimetal conditions. This review summarizes the biosorption potentials of bacterial biomass towards different metal ions, cell wall constituent, biofilm, extracellular polymeric substances (EPS) in metal binding, and the effect of various environmental parameters influencing the metal removal. Suitable mathematical models of biosorption and their application have been discussed to understand and interpret the adsorption process. Furthermore, different desorbing agents and their utilization in heavy metals recovery and regeneration of biosorbent have been summarized. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
48. Bioremediation potential of biofilm forming multi-metal resistant marine bacterium Pseudomonas chengduensisPPSS-4 isolated from contaminated site of Paradip Port, Odisha
- Author
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Priyadarshanee, Monika and Das, Surajit
- Abstract
Biofilm forming and heavy metal resistant marine bacterial strain Pseudomonas chengduensisPPSS-4 was isolated from the contaminated marine sediment of Paradip Port, Odisha, India. The strain showed biofilm formation up to 100 mg/L of multi-metal [Pb(II), Cr(VI), and Cd(II)] supplementation in the culture medium. Scanning electron microscopy (SEM) showed aggregation of rod-shaped cells in the extracellular polymeric substance (EPS) matrix of biofilm. Confocal laser scanning microscopy (CLSM) exhibited a higher nucleic acid to the α-polysaccharide ratio in the biofilm, and the observed thickness was ~21 µm. The metal uptake potential of biofilm culture was higher than planktonic culture both in single and multi-metal solutions. FESEM-EDS analysis revealed the sequestration of multi-metals by bacterial cells and biofilm-EPS. FTIR analysis of bacterial EPS further ensured the interaction of functional groups such as –OH, –NH, and P=O with the metal ions. The maximum removal of Pb, Cr, and Cd by the bacterial biomass was observed at 37°C within 4 h of contact time at pH 6, and 4% salinity for Pb and Cr, and 6% salinity for Cd. The present study revealed that the marine bacterium P. chengduensisPPSS-4 can remove multi-metals, and this bacterium could be efficiently utilized for the remediation of heavy metals in the contaminated environment.
- Published
- 2021
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49. Whole genome characterization and phenanthrene catabolic pathway of a biofilm forming marine bacterium Pseudomonas aeruginosa PFL-P1.
- Author
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Mahto, Kumari Uma and Das, Surajit
- Subjects
PHENANTHRENE ,PSEUDOMONAS aeruginosa ,MARINE bacteria ,POLYCYCLIC aromatic hydrocarbons ,BIOTRANSFORMATION (Metabolism) ,AROMATIC compounds ,GENOME size - Abstract
Pseudomonas aeruginosa is a small rod shaped Gram-negative bacterium of Gammaproteobacteria class known for its metabolic versatility. P. aeruginosa PFL-P1 was isolated from Polycyclic Aromatic Hydrocarbons (PAHs) contaminated site of Paradip Port, Odisha Coast, India. The strain showed excellent biofilm formation and could retain its ability to form biofilm grown with different PAHs in monoculture as well as co-cultures. To explore mechanistic insights of PAHs metabolism, the whole genome of the strain was sequenced. Next generation sequencing unfolded a genome size of 6,333,060 bp encoding 5857 CDSs. Gene ontology distribution assigned to a total of 2862 genes, wherein 2235 genes were allocated to biological process, 1549 genes to cellular component and 2339 genes to molecular function. A total of 318 horizontally transferred genes were identified when the genome was compared with the reference genomes of P. aeruginosa PAO1 and P. aeruginosa DSM 50071. Further comparison of P. aeruginosa PFL-P1 genome with P. putida containing TOL plasmids revealed similarities in the meta cleavage pathway employed for degradation of aromatic compounds like xylene and toluene. Gene annotation and pathway analysis unveiled 145 genes involved in xenobiotic biodegradation and metabolism. The biofilm cultures of P. aeruginosa PFL-P1 could degrade ~74% phenanthrene within 120 h while degradation increased up to ~76% in co-culture condition. GC-MS analysis indicated presence of diverse metabolites indicating the involvement of multiple pathways for one of the PAHs (phenanthrene) degradation. The strain also possesses the genetic machinery to utilize diverse toxic aromatic compounds such as naphthalene, benzoate, aminobenzoate, fluorobenzoate, toluene, xylene, styrene, atrazine, caprolactam etc. Common catabolic gene clusters such as benABCD , xylXYZ and catAB were observed within the genome of P. aeruginosa PFL-P1 which play key roles in the degradation of various toxic aromatic compounds. Image 1 • Marine biofilm forming PAH degrading bacterium Pseudomonas aeruginosa possessed a genome of 6.3 Mbp. • 145 genes accounted for conferring xenobiotic degradation ability. • 269 genes were involved in forming cellular community. • Multiple pathways were found for phenanthrene degradation. [ABSTRACT FROM AUTHOR]
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- 2020
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50. Structural and mechanical characterization of biofilm-associated bacterial polymer in the emulsification of petroleum hydrocarbon
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Vandana and Das, Surajit
- Abstract
The marine bacterium Pseudomonas furukawaiiPPS-19 isolated from the oil-polluted site of Paradip port, Odisha, India, was found to form a strong biofilm in 2% (v/v) crude oil. Confocal Laser Scanning Microscopy (CLSM) revealed biofilm components along with multi-layered dense biofilm of rod-shaped cells with 64.7 µm thickness. Scanning electron micrographs showed similar biofilm architecture covered with a gluey matrix of extracellular polymeric substances (EPS) in the presence of 2% (v/v) crude oil. The architecture of purified EPS was also studied through FESEM that exposed its porous and three-dimensional flakes-like structure. The structural characterization by FTIR revealed that EPS was composed of primary alkane, amines, halide, hydroxyl groups, uronic acid, and saccharides. The XRD profile exhibited an amorphous phase of the EPS with a crystallinity index of 0.336. The EPS showed three-step thermal decomposition and thermal stability up to 600 °C, as confirmed by TGA and DSC thermogram. EPS produced by marine bacterium P. furukawaiiPPS-19 could act as bioemulsifier and showed the highest emulsifying activity of 66.23% on petrol. The emulsifying ability of the EPS was superior to the commercial polymer xanthan. The emulsion also showed high stability with time and temperature exposure. The marine bacterium P. furukawaiiPPS-19 and the EPS complex showed 89.52% degradation of crude oil within 5 days. These properties demonstrated the potential of biofilm-forming marine bacterium as bioemulsifier for its application in the bioremediation of oil-polluted sites.
- Published
- 2021
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