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1. A Single Residue Determines the Cooperative Binding Property of a Primosomal DNA Replication Protein, PriB, to Single-Stranded DNA.

Author

Yen-Hua Huang, Hsin-Hsien Lin, and Cheng-Yang Huang

Subjects
SINGLE-stranded DNA binding proteins, DNA replication, SALMONELLA enterica, GEL permeation chromatography, GLUTARALDEHYDE, NUCLEOTIDES
Abstract

The article presents a study which characterizes and compares the single-stranded DNA (ssDNA)-binding properties of a primosomal DNA replication protein (PriB) Salmonella enterica. The study employs gel filtration, gel shifts, and glutaraldehyde cross-linking. Results proposed the binding modes for forming a stable complex of PriB with ssDNA of 25 nucleotides (nt).

Published
2012
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