13 results on '"Cheng, Yanfen"'
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2. Swainsonine Induces Liver Inflammation in Mice via Disturbance of Gut Microbiota and Bile Acid Metabolism.
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Fu, Keyi, Chen, Xi, Shou, Na, Wang, Zilong, Yuan, Xuefeng, Wu, Dandan, Wang, Qi, Cheng, Yanfen, Ling, Ning, and Shi, Zunji
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- 2023
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3. The uniqueness and superiority of energy utilization in yaks compared with cattle in the highlands: A review
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Liu, Xiaojing, Gao, Jian, Liu, Suozhu, Cheng, Yanfen, Hao, Lizhuang, Liu, Shujie, and Zhu, Weiyun
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Yaks living on the Qinghai-Tibetan Plateau for a long time have evolved a series of mechanisms to adapt to the unique geographical environment and climate characteristics of the plateau. Compared with other ruminants, yaks have higher energy utilization and metabolic efficiency. This paper presents possible mechanisms responsible for the efficient energy utilization, absorption and metabolism resulting from the unique evolutionary process of yaks. It is hoped that the information discussed in this review will give a better insight into the uniqueness and superiority of yaks in regards to energy metabolism and utilization compared with cattle and open new avenues for the targeted regulation of energy utilization pathways of other ruminants.
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- 2023
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4. Taohong Siwu decoction enhances the chemotherapeutic efficacy of doxorubicin by promoting tumor vascular normalization.
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Cheng, Yanfen, Li, Jingjing, Feng, Xi, Wu, Yihan, Wu, Xiaoping, Lau, Benson Wui Man, Ng, Shamay Sheung Mei, Lee, Simon Ming-Yuen, Seto, Sai-Wang, Leung, George Pak-Heng, Hu, Yichen, Fu, Chaomei, Zhang, Siyuan, and Zhang, Jinming
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Instead of completely suppressing blood vessels inside tumors, vascular normalization therapy is proposed to normalize and prune the abnormal vasculature in tumor microenvironment (TME) to acquire a normal and stable blood flow and perfusion. The theoretical basis for the use of "blood-activating and stasis-resolving" formulas in Traditional Chinese Medicine to treat cancer is highly consistent with the principle of vascular normalization therapy, suggesting the potential application of these traditional formulas in vascular normalization therapy. To study the underlying mechanisms of a classical "blood-activating and stasis-resolving" formula, Taohong Siwu decoction (TSD), in enhancing the efficacy of chemotherapy for breast cancer treatment. HUVECs and transgenic zebrafish embryos were used as the major model in vitro. A 4T1 mouse breast cancer model was applied to study tumor vasculature normalization of TSD and the combination effects with DOX. Our data showed that TSD exhibited anti-angiogenic potential in HUVECs and transgenic zebrafish embryos. After 20 days treatment, TSD significantly normalized the tumor vasculature by remodeling vessel structure, reducing intratumoral hypoxia and vessel leakage, and promoting vessel maturation and blood perfusion in 4T1 breast tumor-bearing mice. Moreover, the anti-tumor efficacy of doxorubicin liposome in 4T1 breast tumors was significantly improved by TSD, including the suppression of tumor cell proliferation, angiogenesis, hypoxia, and the increase of cell apoptosis, which is likely through the vascular normalization induced by TSD. TSD also shifted the macrophage polarization from M2 to M1 phenotype in TME during the combination therapy, as evidenced by the reduced number of CD206
+ macrophages and increased number of CD86+ macrophages. Additionally, TSD treatment protected against doxorubicin-induced cardiotoxicity in animals, as evidenced by the reduced cardiomyocytes apoptosis and improved heart function. This study demonstrated for the first time that TSD as a classical Chinese formula can enhance the drug efficacy and reduce the side effects of doxorubicin. These findings can support that TSD could be used as an adjuvant therapy in combination with conventional chemotherapy for the future breast cancer treatment. [Display omitted] [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. Structure and activity relationship analysis of xanthones from mangosteen: Identifying garcinone E as a potent dual EGFR and VEGFR2 inhibitor.
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Li, Jingjing, Nie, Xin, Panthakarn Rangsinth, Wu, Xiaoping, Zheng, Chengwen, Cheng, Yanfen, Shiu, Polly Ho-Ting, Li, Renkai, Lee, Simon Ming-Yuen, Fu, Chaomei, Zhang, Jinming, and Leung, George Pak-Heng
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Xanthones are among the most fundamental phytochemicals in nature. The anti-cancer activities of xanthones and their derivatives have been extensively studied. Recently, we found that garcinone E (GE), an effective anti-cancer phytochemical isolated from mangosteen (Garcinia mangostana l.), showed promising anti-cancer effects in vitro and in vivo. However, little is known about its effects on epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor 2 (VEGFR2) activity. This study aimed to identify potent dual EGFR and VEGFR2 inhibitors from mangosteen-derived xanthones using structure-activity relationship analyses. The interaction of xanthones with EGFR and VEGFR2 was analyzed using molecular docking experiments. The kinase activities of EGFR and VEGFR2 were determined using bioluminescence assays. The rat aortic ring and Matrigel plug angiogenesis assays were used to evaluate blood vessel formation ex vivo and in vivo. A breast tumor-bearing nude mouse model was established to examine the anti-tumor effects of different xanthones. Molecular docking analysis showed that GE bound tightly to EGFR and VEGFR2, with binding energies of -9.73 and -9.56 kcal/mol, respectively. Kinase activity assessment showed that GE strongly inhibited both EGFR and VEGFR2 kinase activity, with IC 50 values of 315.4 and 158.2 nM, respectively. Moreover, GE significantly abolished the EGF- and VEGF-induced phosphorylation of EGFR and VEGFR2, respectively. GE also showed strong inhibitory effects on cancer cell growth, endothelial cell migration, invasion, and tube formation. Ex vivo and in vivo angiogenesis assays showed that GE dose-dependently suppressed blood vessel formation in the rat aorta, Matrigel plugs, and transgenic zebrafish embryos, with the lowest effective concentration of 0.25 μM. Furthermore, GE (2 mg/kg) strongly inhibited tumor growth and reduced tumor weight in MDA-MB-231 breast tumor-xenografted mice. GE significantly reduced microvessel density and downregulated the expression of VEGFR2, EGFR, and Ki67 in tumor tissues. The present study demonstrated that GE was the most potent dual inhibitor of EGFR and VEGFR2 among all xanthones tested. These findings may provide valuable information for the future development of novel and effective dual inhibitors of EGFR and VEGFR2. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2024
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6. Consequences of dietary red pepper and radish oils’ supplementation on growth, carcass traits, blood indices, digestive enzymes activity and intestinal microbial load of broilers
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Arisha, Mahmoud, Attia, Adel I., Reda, Fayiz M., Youssef, Islam M., El dosokey, Azza RF., Swelum, Ayman A., Cheng, Yanfen, and El-Hack, Mohamed E. Abd
- Abstract
This study examined the potential effects of adding red pepper oil (RPO) and radish oil (RO) to broiler diets on the animals' development, immune system performance, and activity of digestive enzymes. A total of 300 1-day of age unsexed Arbor Acre chicks were allotted to five treatment groups in a complete randomized design experiment. Each group had sixty chicks divided into five replicate groups, each of 12 chicks. The 1stgroup (control group) was given a basal diet without supplements. A combination of RPO and RO was added to the basal diet of the remaining four groups at escalating levels: 0.3, 0.6, 0.9, and 1.2g/kg of feed, respectively. Compared to the control, body weight, weight gain, feed intake, and feed conversion ratio significantly improved by adding RPO and RO at 0.6 or 0.9 g/kg of feed. Additionally, with varying RPO and RO levels, there was a considerable reduction in uric acid, creatinine, and aspartate aminotransferase (AST). Birds fed 0.6 g/kg of RPO and RO had lower triglycerides and low-density lipoprotein (LDL). Furthermore, including RPO and RO in the diet mainly impacted the activity of digestive enzymes and antioxidant levels. In addition, thymus and spleen relative weights had been influenced considerably. Additionally, the group that was fed the RPO and RO diet at a 0.9 g/kg level had the highest levels of these immune system markers. Interestingly, chicks fed diets with 0.9 g/kg of RPO and RO showed the lowest levels of E. coli, Salmonella, and coliforms. Additionally, there was a trend of increasing lactic acid bacteria levels with higher concentrations of RPO and RO compared to the control group. We might conclude that the RPO and RO addition in broilers' diets maximizes their production and immunity.
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- 2024
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7. Redox-responsive hyaluronic acid-celastrol prodrug micelles with glycyrrhetinic acid co-delivery for tumor combination therapy
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Fu, Li, Su, Ziye, Wu, Shuyang, Cheng, Yanfen, Hu, Chuan, and Zhang, Jinming
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Combining cytotoxic drugs with tumor microenvironment (TME) modulator agents is an effective strategy to enhance anti-tumor effects. In this study, two natural anti-tumor active ingredients celastrol (CEL) and glycyrrhetinic acid (GA) were combined for tumor treatment. In order to ensure the precise co-delivery and controllable synchronous release of combined drugs to tumors, it is necessary to construct a suitable nano-drug delivery platform. Based on this, we coupled hyaluronic acid (HA) with CEL by amide reaction to obtain an amphiphilic polymer prodrug HA-SS-CEL, and GA was spontaneously loaded into polymer micelles by self-assembly to obtain G/HSSC-M. G/HSSC-M has ideal size distribution, redox-responsive synchronous drug release, enhanced tumor cell internalization and in vivotumor targeting. Compared with free drugs, the construction of multifunctional polymer micelles makes G/HSSC-M show better anticancer effect at the same concentration, and can significantly inhibit the proliferation and migration of HepG2 and 4T1 cells. In the in vivoexperiments, G/HSSC-M inhibited tumor as high as 75.12% in H22 tumor-bearing mice. The mechanism included regulation of M1/M2 macrophage polarization, inhibition of Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) signaling pathway, and remodeling of tumor blood vessels. Therefore, the development of prodrug micelles co-loaded with CEL and GA provides a promising drug co-delivery strategy for combined cancer therapy.
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- 2024
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8. Ensiled diet improved the growth performance of Tibetan sheep by regulating the rumen microbial community and rumen epithelial morphology
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Zhao, Jian, Zhao, Xinsheng, Gao, Jian, Bai, Binqiang, Niu, Jianzhang, Yang, Yingkui, Zhao, Guojun, Wang, Zuojiang, Xu, Zhenhua, Wang, Jilong, Cheng, Yanfen, and Hao, Lizhuang
- Abstract
The aim of this study was to investigate the effects of ensiled agricultural byproducts from Qinghai-Tibet plateau on growth performance, rumen microbiota, ruminal epithelium morphology, and nutrient transport-related gene expression in Tibetan sheep. Fourteen male Tibetan sheep were randomly assigned to one of two diets: an untreated diet (without silage inoculum, CON, n= 7) or an ensiled diet (with silage inoculum, ESD, n= 7). The total experimental period lasted for 84 d, including early 14 d as adaption period and remaining 70 d for data collection. The ESD increased average daily gain (P= 0.046), dry matter intake (P< 0.001), ammonia nitrogen (P= 0.045), microbial crude protein (P= 0.034), and total volatile fatty acids concentration (P< 0.001), and decreased ruminal pH value (P= 0.014). The proportion of propionate (P= 0.006) and the copy numbers of bacteria (P= 0.01) and protozoa (P= 0.002) were higher, while the proportion of acetate (P= 0.028) was lower in the sheep fed ESD compared to CON. Pyrosequencing of the 16S ribosomal RNA gene revealed that ESD increased the relative abundance of Firmicutes, Ruminococcus, Lachnospiraceae_AC2044_group, Lachnospiraceae_XPB1014_group, and Christensenellaceae_R-7_groupin the rumen (P< 0.05), while decreased the relative abundance of Bacteroidota, Prevotellaceae_UCG-003, and Veillonellaceae_UCG-001(P< 0.05). Analyses with PICRUSt2 and STAMP indicated that the propionate metabolism pathway was enriched in the sheep fed ESD (P= 0.026). The ESD increased the rumen papillae height (P= 0.012), density (P= 0.036), and surface area (P= 0.001), and improved the thickness of the total epithelia (P= 0.018), stratum corneum (P= 0.040), stratum granulosum (P= 0.042), and stratum spinosum and basale (P= 0.004). The relative mRNA expression of cyclin-dependent Kinase 2, CyclinA2, CyclinD2, zonula occludens-1, Occludin, monocarboxylate transporter isoform 1 (MCT1), MCT4, sodium/potassium pump, and sodium/hydrogen antiporter 3 were higher in the rumen epithelial of sheep fed ESD than CON (P< 0.05). Conversely, the relative mRNA expressions of Caspase 3and B-cell lymphoma-2 were lower in the sheep fed ESD than CON (P< 0.05). In conclusion, compared with an untreated diet, feeding an ensiled diet altered the rumen microbial community, enhanced nutrient transport through rumen epithelium, and improved the growth performance of Tibetan sheep.This research demonstrated that ensiled diet improved the growth performance of Tibetan sheep by changing the rumen microbial structure and by promoting nutrient transport through rumen epithelium.Tibetan sheep on the Qinghai-Tibet Plateau experience significant nutrient stress while a substantial amount of agricultural byproducts in the region go discarded and wasted. In this study, agricultural byproducts were ensiled and fed to the Tibetan sheep to investigate their effects on growth performance, rumen microorganisms, and nutrient transport through rumen epithelial tissues. Fourteen male Tibetan sheep were randomly assigned to one of two diets: untreated diet (without silage inoculum, CON, n= 7) or ensiled diet (with silage inoculum, ESD, n= 7). After 70 d of feeding, the ESD-fed sheep had a higher body weight than CON. The ensiled diet changed the rumen microbial community and increased the relative abundance of cellulolytic bacteria in the rumen. In addition, the ensiled diet also promoted the development of rumen epithelia and improved the relative expression of gene related to nutrient transport. Overall, the ensiled diet optimized the use of agricultural byproducts and significantly contributed to the production of Tibetan sheep.
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- 2024
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9. Glycyrrhetinic acid suppresses breast cancer metastasis by inhibiting M2-like macrophage polarization via activating JNK1/2 signaling.
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Cheng, Yanfen, Zhong, Xuemei, Nie, Xin, Gu, Huan, Wu, Xiaoping, Li, Renkai, Wu, Yihan, Lv, Kongpeng, Leung, George Pak-Heng, Fu, Chaomei, Lee, Simon Ming-Yuen, Zhang, Jinming, and Li, Jingjing
- Abstract
• GA inhibits TAMs-induced breast cancer growth and metastasis. • GA inhibits macrophage M2 polarization by activating JNK1/2 pathway. • JNK activation is essential for the polarization of M2-like TAMs and TAMs-induced tumor angiogenesis and metastasis. • GA is a safe and potent lead compound for the future development of effective drugs in the treatment of metastatic breast cancer. Breast cancer metastasis is leading cause of cancer death among women worldwide. Tumor-associated macrophages (TAMs) have been considered as potential targets for treating breast cancer metastasis because they promote tumor growth and development. Glycyrrhetinic acid (GA) is one of the most important phytochemicals of licorice which has shown promising anti-cancer efficacies in pre-clinical trials. However, the regulatory effect of GA on the polarization of TAMs remains elusive. To investigate the role of GA in regulating the polarization of M2 macrophages and inhibiting breast cancer metastasis, and to further explore its underlying mechanisms of action. IL-4 / IL-13-treated RAW 264.7 and THP-1 cells were used as the M2-polarized macrophages in vitro. A 4T1 mouse breast cancer model and the tail vein breast cancer metastasis model were applied to study the effect of GA on breast cancer growth and metastasis in vivo. In vitro studies showed that GA significantly inhibited IL-4 / IL 13-induced M2-like polarization in RAW 264.7 and THP-1 macrophages without affecting M1-like polarization. GA strongly decreased the expression of M2 macrophage markers CD206 and Arg-1, and reduced the levels of the pro-angiogenic molecules VEGF, MMP9, MMP2 and IL-10 in M2 macrophages. GA also increased the phosphorylation of JNK1/2 in M2 macrophages. Moreover, GA significantly suppressed M2 macrophage-induced cell proliferation and migration in 4T1 cancer cells and HUVECs. Interestingly, the inhibitory effects of GA on M2 macrophages were abolished by a JNK inhibitor. Animal studies showed that GA significantly suppressed tumor growth, angiogenesis, and lung metastasis in BALB/c mice bearing breast tumor. In tumor tissues, GA reduced the number of M2 macrophages but elevated the proportion of M1 macrophages, accompanied by activation of JNK signaling. Similar results were found in the tail vein breast cancer metastasis model. This study demonstrated for the first time that GA could effectively suppress breast cancer growth and metastasis by inhibiting macrophage M2 polarization via activating JNK1/2 signaling. These findings indicate that GA could be served as the lead compound for the future development of anti-breast cancer drug. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2023
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10. A remote sensing image rotation object detection approach for real-time environmental monitoring
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Tang, Wanyuan, He, Fan, Bashir, Ali Kashif, Shao, Xun, Cheng, Yanfen, and Yu, Keping
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For the problems of resources and environment faced in sustainable development, the use of remote sensing image object detection technology is an effective means to detect and analyze this major problem. Aiming at the problems of low accuracy and slow detection speed of existing remote sensing image rotation object detection algorithms implemented based on Transformer, a remote sensing image rotation object detection method based on dynamic position information Transformer is proposed. Firstly, to improve the detection accuracy, the cross-attention operation of the decoder is improved, and the obtained results are iteratively updated with the position information of the object queries and used as the initial object queries for the next decoder; secondly, to improve the robustness of the network for remote sensing image object detection, the data processing method of image pyramid is designed; Finally, a rotating IoU matching loss function more suitable for oriented object detection is introduced to improve the accuracy of matching the predicted boxed to the true boxed. The detection algorithm proposed in this paper is experimentally verified on DOTA and SSDD datasets, and the average detection accuracy is 73.70% and 90.3%, respectively, which effectively improves the average detection accuracy of Transformer-based rotating object detection algorithm in aerial remote sensing images and has better real-time detection performance.
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- 2023
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11. Swainsonine Induces Liver Inflammation in Mice via Disturbance of Gut Microbiota and Bile Acid Metabolism
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Fu, Keyi, Chen, Xi, Shou, Na, Wang, Zilong, Yuan, Xuefeng, Wu, Dandan, Wang, Qi, Cheng, Yanfen, Ling, Ning, and Shi, Zunji
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Swainsonine induced liver inflammation in livestock; however, the underlying mechanisms, especially the role of bile acids (BAs), in the pathogenesis remained elusive. Here, our results showed that swainsonine induced hepatic inflammation via changing BA metabolism and gut microbiota in mice. Swainsonine significantly upregulated the levels of deoxycholic acid (DCA) and taurine-β-muricholic acid (T-β-MCA) in the serum and liver of mice due to the markedly increased genus Clostridiumand the decreased genus Lactobacillusin the gut. As antagonists of the farnesoid X receptor (FXR), elevated DCA and T-β-MCA inhibited hepatic Fxrgene expression and thus suppressed FXR-SHP signaling and activated hepatic Cyp7a1gene expression, which induced a significant upregulation of the total BA level in serum, contributing to liver inflammation. These findings offer new insights into the underlying mechanisms in which swainsonine induced liver inflammation in mice via the gut–liver axis and suggest that gut microbiota and its metabolite BAs may be underlying triggering factors.
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- 2023
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12. A pH-sensitive supramolecular nanosystem with chlorin e6 and triptolide co-delivery for chemo-photodynamic combination therapy
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Wu, Yihan, Li, Jingjing, Zhong, Xuemei, Shi, Jinfeng, Cheng, Yanfen, He, Chenglin, Li, Jiaxin, Zou, Liang, Fu, Chaomei, Chen, Meiwan, Zhang, Jinming, and Gao, Huile
- Abstract
The combination of Ce6, an acknowledged photosensitizer, and TPL, a natural anticancer agent, has been demonstrated as a useful strategy to reinforce the tumor growth suppression, as well as decrease the systemic side effects compared with their monotherapy. However, in view of the optimal chemo-photodynamic combination efficiency, there is still short of the feasible nanovehicle to steadily co-deliver Ce6 and TPL, and stimuli-responsively burst release drugs in tumor site. Herein, we described the synergistic antitumor performance of a pH-sensitive supramolecular nanosystem, mediated by the host–guest complexing between β-CD and acid pH-responsive amphiphilic co-polymer mPEG-PBAE-mPEG, showing the shell–core structural micelles with the tight β-CD layer coating. Both Ce6 and TPL were facilely co-loaded into the spherical supramolecular NPs (TPL+Ce6/NPs) by one-step nanoprecipitation method, with an ideal particle size (156.0 nm), acid pH-responsive drug release profile, and enhanced cellular internalization capacity. In view of the combination benefit of photodynamic therapy and chemotherapy, as well as co-encapsulation in the fabricated pH-sensitive supramolecular NPs, TPL+Ce6/NPs exhibited significant efficacy to suppress cellular proliferation, boost ROS level, lower MMP, and promote cellular apoptosis in vitro. Particularly, fluorescence imaging revealed that TPL+Ce6/NPs preferentially accumulated in the tumor tissue area, with higher intensity than that of free Ce6. As expected, upon 650-nm laser irradiation, TPL+Ce6/NPs exhibited a cascade of amplified synergistic chemo-photodynamic therapeutic benefits to suppress tumor progression in both hepatoma H22 tumor-bearing mice and B16 tumor-bearing mice. More importantly, lower systemic toxicity was found in the tumor-bearing mice treated with TPL+Ce6/NPs. Overall, the designed supramolecular TPL+Ce6/NPs provided a promising alternative approach for chemo-photodynamic therapy in tumor treatment.
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- 2022
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13. Gut microbiome colonization and development in neonatal ruminants: Strategies, prospects, and opportunities
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Arshad, Muhammad A., Hassan, Faiz-ul, Rehman, Muhammad S., Huws, Sharon A., Cheng, Yanfen, and Din, Ahmad U.
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Colonization and development of the gut microbiome is a crucial consideration for optimizing the health and performance of livestock animals. This is mainly attributed to the fact that dietary and management practices greatly influence the gut microbiota, subsequently leading to changes in nutrient utilization and immune response. A favorable microbiome can be implanted through dietary or management interventions of livestock animals, especially during early life. In this review, we explore all the possible factors (for example gestation, colostrum, and milk feeding, drinking water, starter feed, inoculation from healthy animals, prebiotics/probiotics, weaning time, essential oil and transgenesis), which can influence rumen microbiome colonization and development. We discuss the advantages and disadvantages of potential strategies used to manipulate gut development and microbial colonization to improve the production and health of newborn calves at an early age when they are most susceptible to enteric disease. Moreover, we provide insights into possible interventions and their potential effects on rumen development and microbiota establishment. Prospects of latest techniques like transgenesis and host genetics have also been discussed regarding their potential role in modulation of rumen microbiome and subsequent effects on gut development and performance in neonatal ruminants.
- Published
- 2021
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