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La chirurgie combinée cataracte-vitrectomie se complique fréquemment par l’opacification de la capsule postérieure et par l’apparition de synéchies postérieures de l’iris. Ces complications peuvent être évitées par la mise en place d’un implant « bag in the lens » (BIL). L’objectif de cette étude est de comparer le gain d’acuité visuelle (AV) après chirurgie combinée cataracte-vitrectomie entre un groupe implanté suivant la technique BIL et un groupe avec une implantation dans le sac (IS).

Prostaglandin D2is one of the major prostanoids formed from [14C]arachidonic acid by the central nervous system. The aim of the present study is to specify the prostaglandin D2biosynthetic capacity in the chick spinal cord and to identify the cell type involved in this synthesis. A highly specific and sensitive enzyme immunoassay allowed us to demonstrate that the amount of newly formed prostaglandin D2increases proportionally with the concentration of free arachidonic acid of either exogenous or endogenous origin and reaches concentration values ranging from 10−9to 10−6M. The sites of prostaglandin D2synthesis were localized in Vibratome sections of spinal cord after incubation with antibodies raised against glutathione-independent prostaglandin D synthase; controls were performed with anti-glutathione-dependent prostaglandin D synthase antibodies and non-immune rabbit or goat serum. After immunoprocessing, electron microscope examination revealed that the specific immunoreactivity was confined to small neurons of laminae II and III in the dorsal horn and to motoneurons in the ventral horn of the spinal cord. The immunodeposits were associated with rough endoplasmic reticulum profiles distributed throughout the dorsal horn neurons or restricted to limited subsurface areas of perikarya and dendrites in motoneurons.