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Although typically used to measure dynamic strain from seismic and acoustic waves, Rayleigh‐based distributed acoustic sensing (DAS) is also sensitive to temperature, offering longer range and higher sensitivity to small temperature perturbations than conventional Raman‐based distributed temperature sensing. Here, we demonstrate that ocean‐bottom DAS can be employed to study internal wave and tide dynamics in the bottom boundary layer, a region of enhanced ocean mixing but scarce observations. First, we show temperature transients up to about 4 K from a power cable in the Strait of Gibraltar south of Spain, associated with passing trains of internal solitary waves in water depth <200 m. Second, we show the propagation of thermal fronts associated with the nonlinear internal tide on the near‐critical slope of the island of Gran Canaria, off the coast of West Africa, with perturbations up to about 2 K at 1‐km depth and 0.2 K at 2.5‐km depth. With spatial averaging, we also recover a signal proportional to the barotropic tidal pressure, including the lunar fortnightly variation. In addition to applications in observational physical oceanography, our results suggest that contemporary chirped‐pulse DAS possesses sufficient long‐period sensitivity for seafloor geodesy and tsunami monitoring if ocean temperature variations can be separated. Distributed acoustic sensing (DAS) measures changes in the propagation time of light along finite segments of an optical fiber, which can be caused by both elastic deformations and temperature variations. We present two case studies of long‐period temperature signals recorded with DAS on submarine cables offshore southern Spain and in the Canary Islands. These temperature signals are associated with internal waves, gravity waves that propagate on the ocean's density stratification. We also recover a signal matching the tidal pressure, which likely represents elastic strain, suggesting potential value of ocean‐bottom DAS for seafloor geodesy and tsunami monitoring. Distributed acoustic sensing on seafloor cables can resolve temperature changes associated with internal wave and boundary layer dynamicsWe show temperature transients from solitons in the Strait of Gibraltar and from the propagation of internal tidal fronts at Gran CanariaWe also recover a signal proportional to barotropic tidal pressure including the fortnightly variation Distributed acoustic sensing on seafloor cables can resolve temperature changes associated with internal wave and boundary layer dynamics We show temperature transients from solitons in the Strait of Gibraltar and from the propagation of internal tidal fronts at Gran Canaria We also recover a signal proportional to barotropic tidal pressure including the fortnightly variation

•Hereditary diffuse gastric cancer.•Clinical management.•Germline mutations.

Small-cell carcinomas of the uterine cervix are highly aggressive tumors. Up to 100 of these tumors express at least one neuroendocrine marker such as neuron-specific enolase (NSE), chromogranin A (CgA), and synaptophysin (SYN). In other tumor types such as non-small–cell carcinomas of the lung, colon, and prostate, the presence of these markers has been associated with a better prognosis in some studies, a worsened prognosis in others, or has had no prognostic effect in still other studies. However, little is known about their expression and prognostic significance in the common “non-small–cell” carcinomas of the uterine cervix. The primary tumors of 54 previously untreated patients with histologically confirmed non-small–cell carcinoma of the cervix uteri (squamous carcinoma, adenosquamous carcinoma, and adenocarcinoma) were analyzed by immunohistochemistry for expression of NSE, CgA, and SYN. The expression status was correlated to pathological characteristics and outcome. In addition, the expression of these markers was investigated in cervical carcinoma cell lines. None of the 54 tumors expressed NSE or CgA, although SYN was positive in five tumors (9) of which four were squamous and one was adenocarcinoma. These five patients relapsed within the first 6 months of follow-up and four have died. Among eight cancer cell lines only one was positive for CgA and another one for SYN. We conclude that the neuroendocrine marker SYN is expressed in a small subset of non-small–cell carcinomas of the cervix and its expression seems to correlate with a poor outcome.

The severity of pulmonary fibrosis is the main prognostic factor for survival of patients with interstitial lung diseases (ILD). Unfortunately, lung biopsy, which is the best method to assess fibrosis quantitatively, is done only once during the evolution of the disease. In this study we analyzed the relationship between the degree of fibrosis and the exponential constant k, derived from the lung pressure-volume curve (LPVC) in 33 patients with chronic ILD, 19 with pigeon breeder's disease (PBD), and 14 with idiopathic pulmonary fibrosis (IPF). Pulmonary function tests, including the LPVC, were obtained before biopsy. A semiquantitative histologic assessment of the severity of fibrosis was performed on lung tissues. All patients showed a decrease of total lung capacity, residual volume, compliance, and PaO2. The mean value of the constant k was 0.08 ± 0.06. When expressed as a percent of normal values, 25 patients exhibited values of k lower than 70% of predicted; of the remaining 8 patients whose values were above 70% of predicted, 7 had PBD and only one IPF. On morphologic analysis, 19 patients displayed more than 50% fibrosis. No significant correlations were found between the extent of the lesion or severity of lung fibrosis and the conventional pulmonary function tests. By contrast, a moderate but significant correlation was found between k and the severity of lung fibrosis (r = -0.38, p < 0.05). These findings show that the shape of the LPVC, represented by the constant k, predicts the degree of lung fibrosis and could be useful in the clinical assessment and follow-up of patients with ILD.

The increasing presence of genotoxic chemicals in the aquatic environment has led to the development of bothin vivoandin vitroassays for target species. The fish population represents an important level of aquatic ecosystems that can be threatened by increased environmental pollution. The authors have studied the DNA pattern of the RTG-2 fish cell line, a fibroblast-like cell line, derived from rainbow trout (Oncorhynchus mikyss), to use this cell line as anin vitrosystem to study genotoxicity by means of random amplified polymorphic DNA primers (RAPDs). A constant pattern in the DNA band is essential when an organism or cell line is used to detect DNA alterations produced by genotoxic environmental chemicals. DNA fingerprints with RAPDs were obtained for RTG-2 by testing 26 single and 70 pairwise combinations of primers. Different methods of DNA extraction (chelating resin, salting out, and phenolization), the influence of spectrometric measures at 320 nm in the 260/280 quotient to quantify DNA extracts, genomic DNA and primer concentrations, annealing temperatures, and cell line passage were studied in the cell line characterization. RAPD products were identified by agarose gel electrophoresis. The good results obtained should allow the use of this system as a possible tool for detection of the genotoxicity of aquatic pollutants.