4 results on '"Beccari, Leonardo"'
Search Results
2. Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids
- Author
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Beccari, Leonardo, Moris, Naomi, Girgin, Mehmet, Turner, David A., Baillie-Johnson, Peter, Cossy, Anne-Catherine, Lutolf, Matthias P., Duboule, Denis, and Arias, Alfonso Martinez
- Abstract
The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordinate transcription in space and time. Whereas genetic approaches have revealed important aspects of these processes1, a mechanistic understanding is hampered by the poor experimental accessibility of early post-implantation stages. Here we show that small aggregates of mouse embryonic stem cells (ESCs), when stimulated to undergo gastrulation-like events and elongation in vitro, can organize a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimic embryonic spatial and temporal gene expression. The establishment of the three major body axes in these ‘gastruloids’2,3suggests that the mechanisms involved are interdependent. Specifically, gastruloids display the hallmarks of axial gene regulatory systems as exemplified by the implementation of collinear Hoxtranscriptional patterns along an extending antero-posterior axis. These results reveal an unanticipated self-organizing capacity of aggregated ESCs and suggest that gastruloids could be used as a complementary system to study early developmental events in the mammalian embryo.
- Published
- 2018
- Full Text
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3. Large scale genomic reorganization of topological domains at the HoxDlocus
- Author
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Fabre, Pierre, Leleu, Marion, Mormann, Benjamin, Lopez-Delisle, Lucille, Noordermeer, Daan, Beccari, Leonardo, and Duboule, Denis
- Abstract
The transcriptional activation of HoxDgenes during mammalian limb development involves dynamic interactions with two topologically associating domains (TADs) flanking the HoxDcluster. In particular, the activation of the most posterior HoxDgenes in developing digits is controlled by regulatory elements located in the centromeric TAD (C-DOM) through long-range contacts. To assess the structure–function relationships underlying such interactions, we measured compaction levels and TAD discreteness using a combination of chromosome conformation capture (4C-seq) and DNA FISH. We assessed the robustness of the TAD architecture by using a series of genomic deletions and inversions that impact the integrity of this chromatin domain and that remodel long-range contacts. We report multi-partite associations between HoxDgenes and up to three enhancers. We find that the loss of native chromatin topology leads to the remodeling of TAD structure following distinct parameters. Our results reveal that the recomposition of TAD architectures after large genomic re-arrangements is dependent on a boundary-selection mechanism in which CTCF mediates the gating of long-range contacts in combination with genomic distance and sequence specificity. Accordingly, the building of a recomposed TAD at this locus depends on distinct functional and constitutive parameters.
- Published
- 2017
- Full Text
- View/download PDF
4. The Transcription Factor Encyclopedia
- Author
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Yusuf, Dimas, Butland, Stefanie, Swanson, Magdalena, Bolotin, Eugene, Ticoll, Amy, Cheung, Warren, Cindy Zhang, Xiao, Dickman, Christopher, Fulton, Debra, Lim, Jonathan, Schnabl, Jake, Ramos, Oscar, Vasseur-Cognet, Mireille, de Leeuw, Charles, Simpson, Elizabeth, Ryffel, Gerhart, Lam, Eric, Kist, Ralf, Wilson, Miranda, Marco-Ferreres, Raquel, Brosens, Jan, Beccari, Leonardo, Bovolenta, Paola, Benayoun, Bérénice, Monteiro, Lara, Schwenen, Helma, Grontved, Lars, Wederell, Elizabeth, Mandrup, Susanne, Veitia, Reiner, Chakravarthy, Harini, Hoodless, Pamela, Mancarelli, M, Torbett, Bruce, Banham, Alison, Reddy, Sekhar, Cullum, Rebecca, Liedtke, Michaela, Tschan, Mario, Vaz, Michelle, Rizzino, Angie, Zannini, Mariastella, Frietze, Seth, Farnham, Peggy, Eijkelenboom, Astrid, Brown, Philip, Laperrière, David, Leprince, Dominique, de Cristofaro, Tiziana, Prince, Kelly, Putker, Marrit, del Peso, Luis, Camenisch, Gieri, Wenger, Roland, Mikula, Michal, Rozendaal, Marieke, Mader, Sylvie, Ostrowski, Jerzy, Rhodes, Simon, Van Rechem, Capucine, Boulay, Gaylor, Olechnowicz, Sam, Breslin, Mary, Lan, Michael, Nanan, Kyster, Wegner, Michael, Hou, Juan, Mullen, Rachel, Colvin, Stephanie, Noy, Peter, Webb, Carol, Witek, Matthew, Ferrell, Scott, Daniel, Juliet, Park, Jason, Waldman, Scott, Peet, Daniel, Taggart, Michael, Jayaraman, Padma-Sheela, Karrich, Julien, Blom, Bianca, Vesuna, Farhad, O’Geen, Henriette, Sun, Yunfu, Gronostajski, Richard, Woodcroft, Mark, Hough, Margaret, Chen, Edwin, Europe-Finner, G, Karolczak-Bayatti, Magdalena, Bailey, Jarrod, Hankinson, Oliver, Raman, Venu, LeBrun, David, Biswal, Shyam, Harvey, Christopher, DeBruyne, Jason, Hogenesch, John, Hevner, Robert, Héligon, Christophe, Luo, Xin, Blank, Marissa, Millen, Kathleen, Sharlin, David, Forrest, Douglas, Dahlman-Wright, Karin, Zhao, Chunyan, Mishima, Yuriko, Sinha, Satrajit, Chakrabarti, Rumela, Portales-Casamar, Elodie, Sladek, Frances, Bradley, Philip, and Wasserman, Wyeth
- Abstract
Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded on the principles of open access and collaboration. Our consortium of over 100 researchers has collectively contributed over 130 mini review articles on pertinent human, mouse and rat TFs. Notable features of the TFe website include a high-quality PDF generator and web API for programmatic data retrieval. TFe aims to rapidly educate scientists about the TFs they encounter through the delivery of succinct summaries written and vetted by experts in the field. TFe is available at http://www.cisreg.ca/tfe.
- Published
- 2012
- Full Text
- View/download PDF
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