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ABSTRACTThe Great Basin has traditionally not featured prominently in discussions of how and when the New World was colonized; however, in recent years work at Oregon's Paisley Five Mile Point Caves and other sites has highlighted the region's importance to ongoing debates about the peopling of the Americas. In this paper, we outline our current understanding of Paleoindian lifeways in the northwestern Great Basin, focusing primarily on developments in the past 20 years. We highlight several potential biases that have shaped traditional interpretations of Paleoindian lifeways and suggest that the foundations of ethnographically-documented behavior were present in the earliest period of human history in the region.

Several letters to the editor are presented in response to articles in previous issues including "75 per cent of premises inadequate for future services," in the May 19, 2006 issue, one article on MRCGP exam update by Louise Newson in the May 12, 2006 issue and one article on the British Medical Association's stance on the assisted dying issue in the May19, 2006 issue.

Presents an excerpt of the book `Another World,' by Pat Barker which is about two dysfunctional families.

It has recently been shown that cowpea plants can be infected with a cowpea mosaic virus (CPMV) chimera containing an antigenic site from foot-and-mouth disease virus (Usha et al., Virology197, 366-374, 1993). Analysis of progeny RNA produced during such an infection has revealed that the inserted sequence is rapidly lost during serial passaging, probably by a process of homologous recombination. Using the information gained from this analysis, we have redesigned the chimeras in such a way that they are now genetically stable. The modified constructs have been used to obtain large quantities of purified virus particles expressing epitopes derived from human rhinovirus 14 (HRV-14) and human immunodeficiency virus type 1 (HIV-1). The chimetic virus particles possess the antigenic properties of the inserted sequence and, in the case of the HRV-14-derived construct, it has been shown that the inserted epitope is immunogenic in rabbits. These results demonstrate that CPMV can be used as a high-yielding system for the presentation of foreign peptide sequences.

Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one large open reading frame (ORF) coding for 941 amino acids. The positions of the three coat proteins were located in the putative polyprotein by N-terminal microsequencing and were shown to start at amino acids 287, 495, and 698 for CP-1, CP-2, and CP-3, respectively. The coat proteins are expressed as a polyprotein at the 5' region of the viral RNA genome, and all are cleaved at glutamine carboxy termini, presumably by picornavirus 3C-type of protease(s). Sequence comparisons of coat proteins revealed that there are high amino acid homologies between CP-2 of RTSV and VP3s of encephalomyocarditis virus (EMCV) and Theiler's murine encephalomyelitis virus (TMEV). These results indicate that RTSV is a plant picornavirus.

An excerpt from the book "Toby's Room," by Pat Barker, is presented.