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6 results on '"Barbon, Silvia"'

1. Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia

Author

Bisaccia, Paola, Magarotto, Fabio, D’Agostino, Stefania, Dedja, Arben, Barbon, Silvia, Guidolin, Diego, Liboni, Cristina, Angioni, Roberta, De Lazzari, Giada, Caicci, Federico, Viola, Antonella, Jurga, Marcin, Kundrotas, Gabrielis, Stevens, Dimitri, Mancuso, Domenico, Gramegna, Elisabetta, Seitaj, Bruno, Kashyap, Rudra, De Vos, Beatrice, Macchi, Veronica, Baraldi, Eugenio, Porzionato, Andrea, De Caro, Raffaele, Muraca, Maurizio, and Pozzobon, Michela

Abstract

Oxidative stress and fibrosis are important stress responses that characterize bronchopulmonary dysplasia (BPD), a disease for which only a therapy but not a cure has been developed. In this work, we investigated the effects of mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) on lung and brain compartment in an animal model of hyperoxia-induced BPD. Rat pups were intratracheally injected with MSC-EVs produced by human umbilical cord-derived MSC, following the Good Manufacturing Practice-grade (GMP-grade). After evaluating biodistribution of labelled MSC-EVs in rat pups left in normoxia and hyperoxia, oxidative stress and fibrosis investigation were performed. Oxidative stress protection by MSC-EVs treatment was proved both in lung and in brain. The lung epithelial compartment ameliorated glycosaminoglycan and surfactant protein expression in MSC-EVs-injected rat pups compared to untreated animals. Pups under hyperoxia exhibited a fibrotic phenotype in lungs shown by increased collagen deposition and also expression of profibrotic genes. Both parameters were reduced by treatment with MSC-EVs. We established an in vitro model of fibrosis and another of oxidative stress, and we proved that MSC-EVs suppressed the induction of αSMA, influencing collagen deposition and protecting from the oxidative stress. In conclusion, intratracheal administration of clinical-grade MSC-EVs protect from oxidative stress, improves pulmonary epithelial function, and counteracts the development of fibrosis. In the future, MSC-EVs could represent a new cure to prevent the development of BPD.Graphical AbstractClinical grade MSC-EVs were intratracheally injected in a rat model of broncopulmonary dysplasia. Fibrosis and oxidative stress have been analyzed in lung and brain without and with MSC-EVs.

Published
2024
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4. The Hook Test Is More Accurate Than the Trampoline Test to Detect Foveal Tears of the Triangular Fibrocartilage Complex of the Wrist.

Author

Atzei, Andrea, Luchetti, Riccardo, Carletti, Daniele, Marcovici, Lucian Lior, Cazzoletti, Lucia, and Barbon, Silvia

Abstract

Purpose: To evaluate the accuracy of the trampoline and hook tests, used in the arthroscopic assessment of triangular fibrocartilage complex (TFCC) tears compared with arthroscopic direct visualization of the radiocarpal joint (RCJ) and of the distal radial ulnar joint (DRUJ).Methods: In total, 135 patients (97 male, 38 female, mean age 43.5 years) were divided into 2 groups: (1) 80 patients with chronic ulnar-sided wrist pain and positive fovea sign and (2) 55 patients with other complaints. TFCC was assessed by RCJ and DRUJ arthroscopy and by the trampoline and hook tests to detect rupture of distal and proximal components of the TFCC. Accuracy, specificity, sensitivity, and likelihood ratio of the 2 diagnostic methods were measured and compared, using RCJ and DRUJ arthroscopy as reference.Results: The trampoline and the hook tests showed an overall accuracy of 70.37% and 86.67%, respectively. The accuracy of the trampoline test was similar for distal (69%), proximal (66%), and complete (73%) TFCC tears. The hook test was more accurate when evaluating proximal (97%) and complete (98%) tears, rather than distal lesions (75%). Sensitivity for the trampoline and hook tests was 75.00% and 0.00% (P < .001) for distal tears and 78.85% and 100.00% (P < .001) and 58.33% and 100.00% (P < .001) for complete or isolated proximal tears, respectively. Specificity for the trampoline and hook tests was 67.27% and 96.36% (P < .001) respectively.Conclusions: The trampoline and hook tests can assure accurate diagnosis of peripheral TFCC tear. The hook test shows greater specificity and sensitivity to recognize foveal TFCC tears. Values of positive likelihood ratio suggest a greater probability to detect foveal laceration of peripheral TFCC for the hook test than for the trampoline test. These findings suggest that DRUJ arthroscopy is not necessary to confirm foveal incompetence of the TFCC, if the hook test is positive.Level Of Evidence: Level II, retrospective diagnostic trial. [ABSTRACT FROM AUTHOR]

Published
2021
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5. Spotlight on Circadian Genes and Colorectal Cancer Crosstalk

Author

Rajendran, Senthilkumar, Barbon, Silvia, and Pucciarelli, Salvatore

Abstract

Mammalian physiology is regulated by circadian clock through oscillating feedback loops controlling cellular processes and behaviors. Recent findings have led to an interesting connection between circadian disruption and colorectal cancer progression and incidence through controlling the hallmarks of cancer, namely cell cycle, cell metabolism and cell death. Deeper understanding of the circadian mechanisms that define the colorectal cancer pathophysiology is the need of the hour to define a chronotherapy for improving colorectal cancer patient survival. This review identifies the key areas in which circadian genes interact with cellular pathways to modify the outcome with respect to colorectal cancer incidence and progression.

Published
2021
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6. Time-dependent mechanical behavior of partially oxidized polyvinyl alcohol hydrogels for tissue engineering.

Author

Todros, Silvia, Barbon, Silvia, Stocco, Elena, Favaron, Martina, Macchi, Veronica, De Caro, Raffaele, Porzionato, Andrea, and Pavan, Piero G.

Subjects
POLYVINYL alcohol, TISSUE engineering, BROMINE, STRESS relaxation tests, HYDROGELS, TISSUE scaffolds, PARTIAL oxidation
Abstract

Polyvinyl alcohol (PVA) hydrogels are synthetic polymers which can be used as scaffolds for tissue engineering due to their biocompatibility and large water content. To improve their biodegradation properties, partial oxidation of PVA is achieved by means of different oxidizing agents, such as potassium permanganate, bromine and iodine. The effect of this process on hydrogels mechanical performance has not been fully investigated in view of tissue engineering applications. In this work, the time-dependent mechanical behavior of unmodified and partially oxidized PVA hydrogels is evaluated by means of uniaxial tensile and stress relaxation tests, to evaluate the effect of different oxidizing agents on the viscoelastic response. Tensile tests show an isotropic and almost-incompressible behavior, with a stiffness reduction after PVA oxidation. The time-dependent response of oxidized PVA is comparable to the one of unmodified PVA and is modeled as a quasi-linear viscoelastic behavior. Finite Element (FE) models of PVA samples are developed and numerical analyses are used to evaluate the effect of different strain rates on the mechanical response under uniaxial tension. This model can be exploited to predict the time-dependent mechanical behavior of partially oxidized PVA in tissue engineering application under tensile loading. [Display omitted] • Partial oxidation of PVA hydrogels improves their biodegradation profile. • Partial PVA oxidation causes a minor reduction of tensile stiffness. • The viscoelastic response of PVA hydrogels is comparable before and after oxidation. • A quasi-linear viscoelastic model allows to predict the time-dependent response. [ABSTRACT FROM AUTHOR]

Published
2022
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