Search

Ferrocene (Fc)-based disulfide molecules of various lengths with amino acid scaffolds and alkane or oligo(phenylene-ethynylene) (OPE) bridges are used in a mixed SAM with a di-(ethylene oxide) terminal mercaptoundecanol diluent (PEG2). The relative height of the Fc redox reporter in the SAM is compared to determine if there are protective effects like antifouling and specific detection. The HaloTag-binding motif is used as a proof-of-concept to investigate the electrochemical response to the HaloTag protein due to its known covalent and fast linkage. When the Fc-SAMs are exposed to the HaloTag protein, there are an antifouling nature and more specific detection for the engulfed Fc-based molecules (C6tBu/Halo). The further out the Fc is from the SAM layer, the more nonspecific adsorption is detected. The double layer capacitance (CDL) has the smallest change for the C6tBucontrol (ΔCDL= −0.1 μF cm–2) showing antifouling properties and produces a large change (ΔCDL= 0.9 μF cm–2) as well as a shift in oxidation potential when the active C6Halois exposed to the HaloTag protein (ΔE1/2= 50 ± 10 mV). The remaining Fc molecules are partially in or outside the PEG2layer, allowing more ion penetration/mobility even when the HaloTag protein is bound. Generally, a more disordered environment was observed for the Fc-based molecules when adding the HaloTag ligand, which is evident from a larger Efwhmand higher CDL. Desorption of the SAMs with sodium iodide (NaI) showed retention of the HaloTag protein bound with the corresponding ligand, whereas negative controls did not. Self-assembled monolayers for MALDI mass spectrometry (SAMDI-MS) were used as an orthogonal detection technique to show the qualitative binding of the HaloTag protein to the electrode. Together, these results provide insight into the antifouling and detection methods of engulfing the redox molecules in the SAM diluent.

ABSTRACTThis special edition contributes to the growing scholarship on Australian retail history. It collates a range of work that represents and extends existing themes in Australian retail historiography with the aim of encouraging further work in the field. Topics covered include: Chinese storekeepers in remote regional communities, transnational flows of goods to these communities, colonial arcades and department stores, intercolonial rivalry and boosterism that incentivised the early development of large retail spaces, entrepreneurial business women, Australian milk bars, the influence of diasporic migration on food cultures and leisure activities, advertising in twentieth century department stores, urban planning, and post-war shopping centre development. This article provides an outline of these articles as well as an overview of Australian retail historiography.

ABSTRACTThis article charts the development of regional shopping centres in Sydney’s western suburbs during the 1970s. It shows how rapid population growth on the metropolitan fringe encouraged investment in large-scale retail property development that accommodated the needs of the city’s biggest department store firms. Many of these firms had been involved in the first wave of shopping centre development in middle-ring suburbs in the 1960s. In the 1970s, however, almost all design and construction of regional shopping centres was undertaken by specialist development firms that had honed their capabilities working for retailers during the 1960s. Retailers and developers conducted research to determine ideal site locations, but were guided by the prescriptive hand of metropolitan planning authorities. This meant that Sydney’s retail landscape reflected diverse influences: American style shopping centres were built in locations determined by European planning ideas and occupied by Australian retailers. Planning regimes also constrained subsequent development ensuring that the regional shopping centres built in Sydney’s western suburbs in the 1970s remain at the top of the region’s retail hierarchy today.

This article analyzes the ways that shopping center tenants deployed narratives to encourage government intervention in the Australian retail property sector during the 1980s. Tenants claimed that landlords were abusing their market power through a range of egregious and exploitative practices. Landlords responded with stories of their own, claiming that amateurish retailers were using isolated cases to make broad generalizations about the industry as a whole. Politicians retold retailers’ stories in Parliament, championed small business enterprise as a driver of economic growth, and produced retail leasing legislation aimed at protecting shopping center tenants. In the process, established conceptions of shopping centers were inverted. In the 1960s and 1970s they were seen as bastions of capitalist enterprise constructed by nation-building visionaries. Through stories, retailers captured the cultural legitimacy of entrepreneurship from their landlords, who were characterized as feudal barons blocking the free operation of markets they controlled. Exploring these developments offers new insights into the relational dynamics of preplanned retail environments, expands our understanding of postwar Australian retail history, and contributes to a growing historiography on the role of narrative in business history.

Abstract:This article analyzes the ways that shopping center tenants deployed narratives to encourage government intervention in the Australian retail property sector during the 1980s. Tenants claimed that landlords were abusing their market power through a range of egregious and exploitative practices. Landlords responded with stories of their own, claiming that amateurish retailers were using isolated cases to make broad generalizations about the industry as a whole. Politicians retold retailers' stories in Parliament, championed small business enterprise as a driver of economic growth, and produced retail leasing legislation aimed at protecting shopping center tenants. In the process, established conceptions of shopping centers were inverted. In the 1960s and 1970s they were seen as bastions of capitalist enterprise constructed by nation-building visionaries. Through stories, retailers captured the cultural legitimacy of entrepreneurship from their landlords, who were characterized as feudal barons blocking the free operation of markets they controlled. Exploring these developments offers new insights into the relational dynamics of preplanned retail environments, expands our understanding of postwar Australian retail history, and contributes to a growing historiography on the role of narrative in business history.

The objective of this study was to determine prevalence and perform genomic analysis of Salmonellaspp. and Campylobacterspp. isolated from different stages of an integrated NAE broiler complex. Environmental samples were screened with 3M-Molecular Detection System (MDS) and MDS positive samples were further processed for confirmation of results and identification. Core genome-based phylogenies were built for both bacteria isolated from this study along with selected NCBI genomes. The odds ratios and 95% confidence limits were compared among stages and sample types (α < 0.05) using multivariable model. Based on MDS results, 4% and 18% of total samples were positive for Salmonellaspp. and Campylobacterspp. respectively. The odds of Salmonelladetection in hatchery samples were 2.58 times as likely as compared to its detection in production farms’ samples (P= 0.151) while the odds of Campylobacterdetection in production farms’ samples were 32.19 times as likely as its detection in hatchery (P= 0.0015). Similarly, the odds of Campylobacterdetection in boot swabs, soil, water, and miscellaneous samples were statistically significant (P< 0.05) as compared with fly paper as reference group. The serovars identified for Salmonellawere Typhimurium, Barranquilla, Liverpool, Kentucky, Enteritidis, Luciana, and Rough_O:r:1,5. For Campylobacter,the species identified were Campylobacter jejuniand Campylobacter coli. Phylogeny results show close genetic relatedness among bacterial strains isolated from different locations within the same stage and between different stages. The results show possibility of multiple entry points of such bacteria entering broiler complex and can potentially contaminate the final raw product in the processing plant. It suggests the need for a comprehensive control strategy with strict biosecurity measures and best management practices to minimize or eliminate such pathogens from the poultry food chain.

Cardiovascular disease is a complication of systemic inflammatory diseases including anti-neutrophil cytoplasm antibody-associated vasculitis (AAV). The mechanisms of cardiovascular morbidity in AAV are poorly understood, and risk-reduction strategies are lacking. Therefore, in a series of double-blind, randomized case-control forearm plethysmography and crossover systemic interventional studies, we examined arterial stiffness and endothelial function in patients with AAV in long-term disease remission and in matched healthy volunteers (32 each group). The primary outcome for the case-control study was the difference in endothelium-dependent vasodilation between health and AAV, and for the crossover study was the difference in pulse wave velocity (PWV) between treatment with placebo and selective endothelin-A receptor antagonism. Parallel in vitrostudies of circulating monocytes and platelets explored mechanisms. Compared to healthy volunteers, patients with AAV had 30% reduced endothelium-dependent vasodilation and 50% reduced acute release of endothelial active tissue plasminogen activator (tPA), both significant in the case-control study. Patients with AAV had significantly increased arterial stiffness (PWV: 7.3 versus6.4 m/s). Plasma endothelin-1 was two-fold higher in AAV and independently predicted PWV and tPA release. Compared to placebo, both selective endothelin-A and dual endothelin-A/B receptor blockade reduced PWV and increased tPA release in AAV in the crossover study. Mechanistically, patients with AAV had increased platelet activation, more platelet-monocyte aggregates, and altered monocyte endothelin receptor function, reflecting reduced endothelin-1 clearance. Patients with AAV in long-term remission have elevated cardiovascular risk and endothelin-1 contributes to this. Thus, our data support a role for endothelin-blockers to reduce cardiovascular risk by reducing arterial stiffness and increasing circulating tPA activity.

Salbutamol activates the NaCl cotransporter of the distal convoluted tubule. Salbutamol, in conjunction with high salt intake, induced hypertension in mice, rescued by thiazide therapy. Phosphoproteomics identified protein phosphatase 1/inhibitor 1 as a distinct regulatory node for NaCl cotransporter activation by salbutamol, which did not activate the transporter in inhibitor 1 knockout mice. Salbutamol is widely used in respiratory medicine, and the acquisition of salt sensitivity may be relevant to understanding cardiovascular risk in certain patients.

•Campylobacterprevalence was widespread in commercial broiler production.•Young broilers were highly susceptible to Campylobactercolonization.•Conventional production carried higher AMR incidence rates.•Campylobacter colidisplayed patterns of multidrug resistance.

Patient-derived xenografts (PDXs) are resected human tumors engrafted into mice for preclinical studies and therapeutic testing. It has been proposed that the mouse host affects tumor evolution during PDX engraftment and propagation, affecting the accuracy of PDX modeling of human cancer. Here, we exhaustively analyze copy number alterations (CNAs) in 1,451 PDX and matched patient tumor (PT) samples from 509 PDX models. CNA inferences based on DNA sequencing and microarray data displayed substantially higher resolution and dynamic range than gene expression-based inferences, and they also showed strong CNA conservation from PTs through late-passage PDXs. CNA recurrence analysis of 130 colorectal and breast PT/PDX-early/PDX-late trios confirmed high-resolution CNA retention. We observed no significant enrichment of cancer-related genes in PDX-specific CNAs across models. Moreover, CNA differences between patient and PDX tumors were comparable to variations in multiregion samples within patients. Our study demonstrates the lack of systematic copy number evolution driven by the PDX mouse host.

The endothelin system may be an important player in hypertensive end-organ injury as endothelin-1 increases blood pressure and is pro-inflammatory. The immune system is emerging as an important regulator of blood pressure and we have shown that the early hypertensive response to angiotensin-II infusion was amplified in mice deficient of myeloid endothelin-B (ETB) receptors (LysM-CreEdnrblox/lox). Hypothesizing that these mice would display enhanced organ injury, we gave angiotensin-II to LysM-CreEdnrblox/lox and littermate controls (Ednrblox/lox) for six weeks. Unexpectedly, LysM-CreEdnrblox/lox mice were significantly protected from organ injury, with less proteinuria, glomerulosclerosis and inflammation of the kidney compared to controls. In the eye, LysM-CreEdnrblox/lox mice had fewer retinal hemorrhages, less microglial activation and less vessel rarefaction. Cardiac remodeling and dysfunction were similar in both groups at week six but LysM-CreEdnrblox/lox mice had better endothelial function. Although blood pressure was initially higher in LysM-CreEdnrblox/lox mice, this was not sustained. A natriuretic switch at about two weeks, due to enhanced ETBsignaling in the kidney, induced a hypertensive reversal. By week six, blood pressure was lower in LysM-CreEdnrblox/lox mice than in controls. At six weeks, macrophages from LysM-CreEdnrblox/lox mice were more anti-inflammatory and had greater phagocytic ability compared to the macrophages of Ednrblox/lox mice. Thus, myeloid cell ETBreceptor signaling drives this injury both through amplifying hypertension and by inflammatory polarization of macrophages.

Hopeful Journeys Educational Center faces a daily task of assigning tutors to students subject to myriad complex rules and restrictions. The organization’s mission, which is to provide individualized education to students with autism spectrum disorders and other developmental disabilities, as well as its limited operating budget and day-to-day resource/demand variability, makes this a uniquely challenging scheduling problem. When we first communicated with Hopeful Journeys, the organization was in critical need of an efficient methodology for producing daily schedules to replace its existing time-consuming and error-prone manual approach. This paper describes the fully open-source, Excel-based optimization tool we developed to support Hopeful Journeys’ mission. Our work illustrates the potential to use freely available operations research tools within a “rapid prototyping” approach to provide immediate impact to organizations that lack the resources to utilize commercial software or professional consultants.

The biomimetic route to inorganic synthesis presents an opportunity to produce complex materials with superior properties under ambient conditions and from nontoxic precursors. While there has been significant progress in using solid-binding peptides (SBPs), proteins, and organisms to produce a variety of inorganic and hybrid structures, it has been more challenging to understand the interplay of solution conditions and solid-binding peptide (SBP) sequence, structure, and self-association on synthetic outcomes. Here, we show that fusing the Car9 silica-binding peptide—but not the silaffin-derived R5 peptide—to superfolder green fluorescent protein (sfGFP) enhances the ability of micromolar concentrations of protein to induce rapid titania (TiO2) precipitation from acidified solutions of tetrakis(di-lactato)-oxo-titanate (TiBALDH). TiO2is produced stoichiometrically and although predominantly amorphous, contains nanosized anatase and monoclinic TiO2(B) inclusions. Remarkably, the phase of these nanocrystallites can be tuned from about 80% TiO2(B) to about 65% anatase by using Car9 mutants impaired in their ability to drive the formation of higher-order sfGFP-Car9 oligomers. Our results suggest that the presentation of multiple basic side chains in an extended plane formed by SBP self-association is critical to template the formation of monoclinic crystallites and underscore the subtle influence that single or dual substitutions in dodecameric SBPs can exert on the yield and crystallinity of biomineralized inorganics.

We describe a systematic study of the influence of halides and solvents on the optical properties of EuII-containing complexes in solution starting from well-defined crystalline precursors. Anionic halides, chloride and bromide, blue-shift the spectroscopic properties of EuII, whereas neutral ligands, methanol and acetonitrile, cause a red shift. This system provides evidence that EuIIhas a stronger affinity for chloride, and to some extent bromide, relative to acetonitrile but not methanol. We also describe a simple procedure using an ion-exchange resin for the exchange of iodide counterions to hexafluorophosphate. These findings are a step toward designing ligands that can tune the optical properties of EuII-containing complexes for solution-based applications.

Salmonellais one of the top causes for bacterial foodborne infections in the United States, emphasizing the importance of controlling this pathogen for protecting public health. Poultry and poultry products are commonly associated with Salmonella,and interventions during production and processing are necessary to manage the risk of infection due to consumption of poultry products. In recent times, the demand for organic and antibiotic-free poultry has increased owing to consumer perceptions and concerns of increasing prevalence of antimicrobial-resistant (AMR) pathogens. However, the microbiological effect of these management practices is not clear. This study was conducted to determine the difference in the AMR of Salmonellaisolated from poultry processed conventionally and organically. Fecal samples, carcass rinses, and environmental samples were collected over 1 year and analyzed for the prevalence of Salmonellaand AMR. Results of this experiment showed that organic chickens were associated with statistically higher levels of Salmonelladuring early processing steps. However, no difference in Salmonellaprevalence was observed between organic and conventional carcasses postchill. In addition, for most antimicrobial agents tested, prevalence of AMR Salmonellain conventional processing was lower in this study than was reported by the National Antimicrobial Resistance Monitoring System for chickens at slaughter. These observations indicate that organic methods may introduce greater risk of Salmonellacontamination; however, proper interventions during processing can abate this risk. In addition, this study supports the assertion that raising chickens without the use of antibiotics may result in lower prevalence of AMR Salmonella.

Due to the importance of both visible-light luminescence and lanthanides in modern society, the influence of the ligand environment on complexes of YbIIwere studied and compared with analogous complexes of EuII. Four ligands with systematically varied electronic and steric characteristics were used to probe the coordination environment and electronic and redox properties of the corresponding YbII-containing complexes. Strong-field nitrogenous donors gave rise to bathochromic shifts, leading to visible-light absorption by YbII. Trends in properties across the series of YbII-containing complexes were compared to trends reported for the analogous EuII-containing complexes, revealing the translatability of coordination environment effects across the divalent lanthanide series. These studies provide valuable information regarding the behavior of small and medium-sized divalent lanthanides outside of the solid state.

The sodium-chloride cotransporter (NCC) in the distal convoluted tubule contributes importantly to sodium balance and blood pressure (BP) regulation. NCC phosphorylation determines transport activity and has a diurnal rhythm influenced by glucocorticoids. Disturbing this rhythm induces “nondipping” BP, an abnormality that increases cardiovascular risk. The receptor through which glucocorticoids regulate NCC is not known. In this study, we found that acute administration of corticosterone to male C57BL6 mice doubled NCC phosphorylation without affecting total NCC abundance in both adrenalectomized and adrenal-intact mice. Corticosterone also increased the whole kidney expression of canonical clock genes: period circadian protein homolog 1 (Per1), Per2, cryptochrome 1, and aryl hydrocarbon receptor nuclear translocator-like protein 1. In adrenal-intact mice, chronic blockade of glucocorticoid receptor (GR) with RU486 did not change total NCC but prevented corticosterone-induced NCC phosphorylation and activation of clock genes. Blockade of mineralocorticoid receptor (MR) with spironolactone reduced the total pool of NCC but did not affect stimulation by corticosterone. The diurnal rhythm of NCC phosphorylation, measured at 6-h intervals, was blunted by chronic GR blockade, and a similar dampening of diurnal variation was seen in GR heterozygous null mice. These effects on NCC phosphorylation did not reflect altered rhythmicity of plasma corticosterone or serum and glucocorticoid-induced kinase 1 activity. Both mineralocorticoids and glucocorticoids emerge as regulators of NCC, acting via distinct receptor pathways. MR activation provides maintenance of the NCC protein pool; GR activation dynamically regulates NCC phosphorylation and establishes the diurnal rhythm of NCC activity. This study has implications for circadian BP homeostasis, particularly in individuals with abnormal glucocorticoid signaling as is found in chronic stress and corticosteroid therapy.

Flue Gas Desulfurization (FGD) gypsum is a byproduct of the coal-fired power plant process commonly used to remove sulfur dioxide emissions from the flue gas. FGD gypsum has numerous industrial, agricultural, and environmental applications. This study aimed to explore a novel approach involving the use of FGD gypsum combined with different litter treatments as bedding for broiler production. It focused on performance metrics, including adjusted feed conversion ratio (AFCR) and average body weight (BW), foot pad dermatitis (FPD), and fear response over five consecutive flocks. A total of 1,800 one-day-old Ross 708 chicks were randomly assigned to 24 pens (75 birds/pen), divided into six treatment groups (4 pens/treatment), with five replications and raised until 42 days old (d). Treatments were gypsum that was decaked (D), rotovated (E), and rotovated then windrowed (F) between flocks. Control treatments using pine shavings were decaked (A), rotovated (B), and windrowed post-rotovating (C). AFCR, average BW, and mortality were used as a measure of production. FPD scores were taken on d42 using a scale of 0 (absence), 1 (mild), and 2 (severe). Response to observer and human approach test were used to measure fear response. Data were analyzed as a two-way ANOVA (Proc Glimmix) for the main effects of bedding type and litter treatment. Means were identified using Tukey's HSD. No effect of bedding type or litter treatment was found for AFCR, BW, or mortality. FPD scores 2 and 1, were higher with pine shavings than gypsum (P = 0.01 and P =0.01, respectively). While FPD scores 0 were higher for gypsum than the pine shaving (P = 0.01). No difference in fear response was found among birds raised on any of the gypsum litter treatments and any of the pine shaving litter treatments. Overall, the use of gypsum as bedding results in equivalent production and fear response to pine shavings, while increasing FPD quality when compared to pine shaving.

This article reports on the methodology for recruiting oral history interviewees for a project on the history of shopping center development in Australia. Snowball sampling produced a data set that added value to extant archival and media sources, rendering detailed information about firm operations, strategies, and innovations. The construction of the sample was also revealing of the industry itself, with interviewee referral chains connecting individuals across firms, industry sectors, and generations. These links marked not only social connections but also paths through which knowledge was transferred via mentoring or shared experience, personal friendships that developed in workplaces, and professional contacts that evolved within a complex and constantly changing industry. Snowball sampling, though, also proved to be selective and contingent on the social networks of interviewees, requiring ongoing management of the chain referral process.

This article analyses the evolving relationship between mainstream oral history and business oral history, and explores the ways in which the latter has been deployed and discussed in business history journals. Business historians have, until relatively recently, tended to utilize oral history as a means to fill gaps in the archive. Interviews thus made important contributions to business history studies, but much of their potential remained untapped. Recent critical engagement with issues of methodology and interpretation has seen a discernible shift in the ways that oral history is being understood by business historians. This article outlines this evolution and the possibilities that it raises for both business and oral history.

Abstract:This article reports on the methodology for recruiting oral history interviewees for a project on the history of shopping center development in Australia. Snowball sampling produced a data set that added value to extant archival and media sources, rendering detailed information about firm operations, strategies, and innovations. The construction of the sample was also revealing of the industry itself, with interviewee referral chains connecting individuals across firms, industry sectors, and generations. These links marked not only social connections but also paths through which knowledge was transferred via mentoring or shared experience, personal friendships that developed in workplaces, and professional contacts that evolved within a complex and constantly changing industry. Snowball sampling, though, also proved to be selective and contingent on the social networks of interviewees, requiring ongoing management of the chain referral process.

Abstract:This article analyses the evolving relationship between mainstream oral history and business oral history, and explores the ways in which the latter has been deployed and discussed in business history journals. Business historians have, until relatively recently, tended to utilize oral history as a means to fill gaps in the archive. Interviews thus made important contributions to business history studies, but much of their potential remained untapped. Recent critical engagement with issues of methodology and interpretation has seen a discernible shift in the ways that oral history is being understood by business historians. This article outlines this evolution and the possibilities that it raises for both business and oral history.

The technological divide that incarcerated students experience when undertaking tertiary studies via Distance Education (DE) separates them from other university students. The aim of this article is to research the problems incarcerated students have accessing technology for the purpose of study and to understand the technological facilities needed to support their learning. Quantitative and qualitative survey data was collected for the study from students in the New South Wales Corrective Services (NSWCS) and from Prison Education Officers (PEOs) employed by NSWCS. The surveys explored the educational and technological concerns, present and future, of this cohort of diverse students and examined the perspectives of the PEOs. Findings from the research highlight that incarcerated students engaged in study felt it made them feel positive about their future, inspired them to continue studying after their prison term, and they would recommend further study to fellow prisoners. Preparing those in Corrective Services (CS) for life after incarceration is essential for reducing recidivism. As this article reveals, educating those in the prison system may contribute to enhanced social and cultural capital and thus it is an important consideration for government.

Salmonellaand Campylobacterare common foodborne pathogens in chickens, but their persistence mechanisms within flocks are not fully understood. In this study, 4 groups of SPF Leghorn chickens (n = 50) were orally inoculated with 108SalmonellaEnteritidis and 108Campylobacter jejuni, housed in BSL-2 rooms inside containers with autoclaved bedding and beetles (n = 200). Phase I (wk 1–3): the infected chickens remained in the containers and were then euthanized while beetles and litter remained in the container (group A), beetles were removed and litter remained in the container (group B), beetles remained and litter was removed (group C), and beetles and litter were removed (group D). Phase II (wk 5–7): autoclaved bedding was added to containers in groups C and D, and new SPF chickens (n = 50) were introduced and kept. Phase III (wk 8–20): all chickens were euthanized, and the litter and/or beetles remained in the containers for 17 wk. The prevalence of SalmonellaEnteritidis and Campylobacterwas significantly higher when detected by PCR compared to culture. In phase II, when infected chickens were removed and new chickens were introduced, 1 fecal sample in group B and 3 litter samples in groups B and C were found positive for SalmonellaEnteritidis, and Campylobacterwas still detected in groups A, B, and C litter samples, but not in beetles. In phase III, when all chickens were removed, SalmonellaEnteritidis was identified in beetle samples from group A and the litter samples of all tested groups A, B, and C, and C. jejuniwas positive in litter samples from groups A and B but not in the beetle. Sixty-nine days after removing infected chickens, culturable Salmonellawas still found in beetles. Salmonellaand Campylobacterwere detectable in litter up to 127 d after removing infected chickens. This study highlights the transmission of Salmonellaand Campylobactervia beetles and litter to new flocks in successive rearing cycles. Intensive control programs should target insect exclusion and implement strict poultry litter management or litter changes between flocks.

A successful hatch has a considerable economic impact on all poultry companies. The aim of the current study was to describe the possible effects of shell translucency (T score) and coloration lightness (L* value) on shell thickness, hatchability, and chick weight. A total of 4,320 eggs from 4 commercial Ross 708 breeder flocks (50–55-wk old) were used. Eggs were selected for T score and L* value. A 3-point subjective scoring system was used for T score (1 = low, 2 = medium, 3 = high), and an electronic colorimeter for L* value, sorting the eggs as light (avg. L* = 80.7) or dark (avg. L* = 76.0). Data were analyzed using the GLIMMIX procedure of SAS (V9.4) and Tukey's HSD test was performed to separate means, a significant difference was considered when P≤ 0.05. Results suggest that the color of the eggshell was related to the egg weight on the day of collection (P = 0.0056) and at transfer (P = 0.0211), in both cases dark eggs were 0.6 g heavier than light eggs. Dark eggs had a 3.8% increased hatchability of egg set (P = 0.0481) and yielded 6 µm thicker shells (P = 0.0019) when compared to light eggs. Regarding translucency, egg weight at transfer was 0.8 g heavier for T score 1 eggs compared to T score 3 (P = 0.0358). The translucency score of 1 had a 6.9% higher hatchability of eggs set (P = 0.0127) and 0.7 g heavier chick weight (P = 0.0385) compared to T score 3. However, T score 1 eggs had shells 28 µm thinner than the T score 2 and 34 µm thinner than T score 3 (P< 0.0001). An interaction effect was observed for eggshell thickness, L* value, and T score, where eggs classified as light with T score 1 had thinner eggshells compared to those that were dark with T score 3 (P = 0.0292). These results suggest that eggshell translucency and coloration lightness can be good noninvasive indicators of eggshell thickness, hatchability, and chick weight in broiler breeder flocks.

We have conducted Raman spectroscopy experiments on liquid ethane (C2H6) at 300 K, obtaining a large amount of data at very high resolution. This has enabled the observation of Raman peaks expected but not previously observed in liquid ethane and a detailed experimental study of the liquid that was not previously possible. We have observed a transition between rigid and nonrigid liquid states in liquid ethane at ca. 250 MPa corresponding to the recently proposed Frenkel line, a dynamic transition between rigid liquid (liquidlike) and nonrigid liquid (gaslike) states beginning in the subcritical region and extending to arbitrarily high pressure and temperature. The observation of this transition in liquid (subcritical) ethane allows a clear differentiation to be made between the Frenkel line (beginning in the subcritical region at higher density than the boiling line) and the Widom lines (emanating from the critical point and not existing in the subcritical region). Furthermore, we observe a narrow transition at ca. 1000 MPa to a second rigid liquid state. We propose that this corresponds to a state in which orientational order must exist to achieve the expected density and can view the transition in analogy to the transition in the solid state away from the orientationally disordered phase I to the orientationally ordered phases II and III.

Deficits in goal-directed motivation represent a debilitating symptom for many patients with schizophrenia. Impairments in motivation can arise from deficits in processing information about effort and or value, disrupting effective cost-benefit decision making. We have previously shown that upregulated dopamine D2 receptor expression within the striatum (D2R-OE mice) decreases goal-directed motivation. Here, we determine the behavioral and neurochemical mechanisms behind this deficit. Female D2R-OE mice were tested in several behavioral paradigms including recently developed tasks that independently assess the impact of Valueor Effortmanipulations on cost-benefit decision making. In vivo microdialysis was used to measure extracellular dopamine in the striatum during behavior. In a value-based choice task, D2R-OE mice show normal sensitivity to changes in reward value and used reward value to guide their actions. In an effort-based choice task, D2R-OE mice evaluate the cost of increasing the number of responsesgreater relative to the effort cost of longer duration responsescompared to controls. This shift away from choosing to repeatedly execute a response is accompanied by a dampening of extracellular dopamine in the striatum during goal-directed behavior. In the ventral striatum, extracellular dopamine level negatively correlates with response cost in controls, but this relationship is lost in D2R-OE mice. These results show that D2R signaling in the striatum, as observed in some patients with schizophrenia, alters the relationship between effort expenditure and extracellular dopamine. This dysregulation produces motivation deficits that are specific to effort but not value-based decision making, paralleling the effort-based motivational deficits observed in schizophrenia.

AbstractHusbandry practices for laying hens in commercial egg production is a topic of interest from a social, economic, and regulatory standpoint. Animal welfare concerns regarding the use of conventional cages have arisen and consumer perceptions of hen welfare have led to a higher demand for cage-free eggs. The aim of this study was to assess the impact of housing systems on prevalence, persistence, and antimicrobial resistance (AMR) of Campylobacterfrom laying hens and shell eggs. A total of 425 samples were collected over a 10-month period from the North Carolina Layer Performance and Management Test and Campylobacterisolates were identified by serological, biochemical, and molecular tests. Genetic variability was evaluated using pulsed-field gel electrophoresis (PFGE) and AMR testing was performed. Prevalence of Campylobacterspp. ranged from 11.1% in the enrichable cages to 19.7% in the conventional systems. A greater prevalence of Campylobacterwas found in the fecal swab samples from free-range birds compared with those of birds housed in the more intensive housing systems (p> 0.05). Overall, 72 isolates were confirmed as Campylobacterspp. by PCR. More than 90% of the isolates (n= 66) were identified as Campylobacter jejuni, followed by Campylobacter coli(n= 6). C. jejuniisolates displayed high levels of resistance to tetracycline (67%). Genetic variability of Campylobacterwas high, with more than 20 PFGE patterns identified. Pattern “a” comprised 42% of isolates from all housing systems and was also the most persistent. This study suggests that housing systems of laying hens used for commercial shell egg production may impact the rate of Campylobactershedding by layers. Isolation rates and tetracycline resistance levels of this pathogen are still of concern, emphasizing the need for well-implemented biosecurity measures on the farm.

Decimal reduction time (D-value) was calculated for six non-O157 Shiga toxin–producing Escherichia coli(STEC) in a laboratory medium and ground beef. For the laboratory medium, an overnight culture of each strain of STEC was divided into 10-mL sample bags and heated in a water bath for a specific time on the basis of the temperatures. Survival curves were generated by plotting the surviving bacterial population against time, and a linear-log primary model was used to estimate the D-values from survival curves. The z-values (the temperature raised to reduce the D-value by one-tenth) were calculated by plotting the log D-values against temperature. Similarly, for ground beef, six fat contents, 5, 10, 15, 20, 25, and 30% of ground beef were formulated for this study. Inoculated meat was divided into 5-g pouches and submerged in a water bath set at specific temperatures (55, 60, 65, 68, and 71.1°C). The average D-value for these strains in a laboratory medium was 17.96 min at 55°C, which reduced significantly (P< 0.05) to 1.58 min at 60°C, and then further reduced (P< 0.05) to 0.46 min at 65°C. In ground beef, a negative correlation (P< 0.05) between fat content of ground beef and D-values was observed at 55°C. However, at temperatures greater than 60°C, there was no impact (P> 0.05) of fat content of ground beef on the thermal resistance of non-O157 STECs. Irrespective of the fat content of ground beef, the D-values ranged from 15.93 to 11.69, 1.15 to 1.12, and 0.14 to 0.09 min and 0.05 at 55, 60, 65, and 68°C, respectively. The data generated from this study can be helpful for the meat industry to develop predictive models for thermal inactivation of non-O157 STECs in ground beef with varying fat content.