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Genome-wide analysis has demonstrated both health and social benefits. However, large scale sharing of such data may reveal sensitive information about individuals. One of the emerging challenges is identity tracing attack that exploits correlations among genomic data to reveal the identity of DNA samples. In this paper, we first demonstrate that the adversary can narrow down the sample's identity by detecting his/her genetic relatives and quantify such privacy threat by employing a Shannon entropy-based measurement. For example, we exemplify that when the dataset size reaches 30% of the population, for any target from that population, the uncertainty of the target's identity is reduced to merely 2.3 bits of entropy (i.e., the identity is pinned down within 5 people). Direct application of existing approaches such as differential privacy (DP), secure multiparty computation (MPC) and homomorphic encryption (HE) may not be applicable to this challenge in genome-wide analysis because of the compromise on utility (i.e., accuracy or efficiency). Towards addressing this challenge, this paper proposes a framework named $\upsilon$υFrag to facilitate privacy-preserving data sharing and computation in genome-wide analysis. $\upsilon$υFrag mitigates privacy risks by using a vertical fragmentation to disrupt the genetic architecture on which the adversary relies for identity tracing without sacrificing the capability of genome-wide analysis. We theoretically prove that it preserves the correctness of the primitive functionalities and algorithms ranging from basic summary statistics to advanced neural networks. Our experiments demonstrate that $\upsilon$υFrag outperforms secure multiparty computation (MPC) and homomorphic encryption (HE) protocols, with a speedup of more than 221x for training neural networks, and also traditional non-private algorithms and a state-of-the-art noise-based differential privacy (DP) solution in most settings.

The goal of this study was to investigate the effects of early fecal microbial transfer (FMT) on the microflora of recipient piglets, where Yorkshire newborn piglets and Min sows (an indigenous pig breed in China) were used as the fecal recipients and donors, respectively, to reveal the changes in immunity and development-related functions of the intestinal mucosa driven by FMT. The recipient group was inoculated with fecal microbial fluids from days 1 to 10. On day 21, the relative abundance of the Proteobacteriawas reduced; the concentrations of immunoglobulin M (IgM) and immunoglobulin G (IgG) in the jejunal mucosa, and that of IgG in the ileal mucosa of the recipient group, were increased (P< 0.05). On day 40, the relative abundance of the Firmicutes in the recipient group was increased, while that of Bacteroideswas decreased. The concentrations of IgG and IgM in the ileal mucosa of the recipient group were increased. FMT protected the intestine by modulating the antimicrobial peptides of the intestinal mucosa (P< 0.05). The results of this study revealed that early FMT can improve the gut microbiota, intestinal mucosal immunity, and intestinal development-related functions of Yorkshire piglets.

Nisin (Ni) is a polypeptide bacteriocin produced by lactic streptococci(probiotics) that can inhibit the majority of gram-positive bacteria, and improve the growth performance of broilers, and exert antioxidative and anti-inflammatory properties. The present study investigated the potential preventive effect of Nisin on necrotic enteritis induced by Clostridium perfringens(Cp) challenge. A total of 288 Arbor Acres broiler chickens of 1-d-olds were allocated using 2 × 2 factorial arrangement into four groups with six replicates (12 chickens per replicate), including: (1) control group (Con, basal diet), (2) Cp challenge group (Cp, basal diet + 1.0 × 108CFU/mL Cp), (3) Ni group (Ni, basal diet + 100 mg/kg Ni), and (4) Ni + Cp group (Ni + Cp, basal diet + 100 mg/kg Ni + 1.0 × 108CFU/mL Cp). The results showed that Cp challenge decreased the average daily gain (ADG) of days 15 to 21 (P<0.05) and increased interleukin-6 (IL-6) content in the serum (P< 0.05), as well as a significant reduction in villus height (VH) and the ratio of VH to crypt depth (VCR) (P<0.05) and a significant increase in crypt depth (CD) of jejunum (P<0.05). Furthermore, the mRNA expressions of Occludin and Claudin-1 were downregulated (P<0.05), while the mRNA expressions of Caspase3, Caspase9, Bax, and Bax/Bcl-2 were upregulated (P<0.05) in the jejunum. However, the inclusion of dietary Ni supplementation significantly improved body weight (BW) on days 21 and 28, ADG of days 15 to 21 (P<0.05), decreased CD in the jejunum, and reduced tumor necrosis factor-α (TNF-α) content in the serum (P<0.05). Ni addition upregulated the mRNA levels of Claudin-1 expression and downregulated the mRNA expression levels of Caspase9 in the jejunum (P<0.05). Moreover, Cp challenge and Ni altered the cecal microbiota composition, which manifested that Cp challenge decreased the relative abundance of phylum Fusobacteriotaand increased Shannon index (P<0.05) and the trend of phylum Proteobacteria(0.05

The intestinal architecture of piglets is vulnerable to disruption during weaning transition and leads to diarrhea, frequently accompanied by inflammation and metabolic disturbances (including amino acid metabolism). Tryptophan (Trp) plays an essential role in orchestrating intestinal immune tolerance through its metabolism via the kynurenine, 5-hydroxytryptamine, or indole pathways, which could be dictated by the gut microbiota either directly or indirectly. Emerging evidence suggests a strong association between piglet diarrhea and Trp metabolism. Here we aim to summarize the intricate balance of microbiota-host crosstalk by analyzing alterations in both the host and microbial pathways of Trp and discuss how Trp metabolism may affect piglet diarrhea. Overall, this review could provide valuable insights to explore effective strategies for managing piglet diarrhea and the related challenges.

The objective of this study was to investigate the effects of diets supplemented with sodium stearoyl-2-lactylate (SSL), polyglycerol fatty acid ester (PGFE), and combined emulsifiers (0.02% SSL and 0.08% PGFE) on growth performance, nutrient digestibility, and plasma lipid profiles in weaned piglets and to further evaluate the possible effects of feeding exogenous emulsifiers on digestive enzyme activities and liver bile acid (BA) metabolism. Twenty-eight barrows (age at 35 d, Duroc × Landrace × Yorkshire) with an initial BW of 10.13 ± 0.16 kg were randomly assigned to 4 dietary treatment groups (7 pigs/treatment). Dietary treatment groups included the following: 1) basal diet (Control, CTR); 2) basal diet with 0.1% SSL (SSL); 3) basal diet with 0.1% PGFE (PGFE); and 4) basal diet with 0.08% PGFE+0.02% SSL (PG-SL). SSL diet increased ADG and ADFI of piglets during day 0 to 17 (P < 0.05) compared with the CTR treatment. Piglets fed emulsifier diets experienced a significant improvement in the digestibility of nutrients (DM, CP, ether extract, energy, calcium, and phosphorus) during the first 17 d (P < 0.05). The level of low-density lipoprotein cholesterol (LDL-C) was lower in the PGFE and PG-SL treatment groups than in the CTR treatment group (P < 0.05). Feeding emulsifier diets increased the lipase activity of the pancreas when compared with the CTR diet (P < 0.05). Moreover, the emulsifier diets significantly increased the mRNA expression of FXR (P < 0.05) and decreased the mRNA expression of CYP27A1 (P < 0.05) in the liver. In conclusion, the addition of emulsifiers improved nutrient digestibility and increased the mRNA expression of FXR BA receptors while inhibiting the mRNA expression of BA biosynthesis by CYP27A1 in weanling piglets.

As feature-rich Android applications (apps for short) are increasingly popularized in security-sensitive scenarios, methods to verify their security properties are highly desirable. Existing approaches on verifying Android apps often have limited effectiveness. For instance, static analysis often suffers from a high false-positive rate, whereas approaches based on dynamic testing are limited in coverage. In this work, we propose an alternative approach, which is to apply the software model checking technique to verify Android apps. We have built a general framework named DroidPF upon Java PathFinder (JPF), towards model checking Android apps. In the framework, we craft an executable mock-up Android OS which enables JPF to dynamically explore the concrete state spaces of the tested apps; we construct programs to generate user interaction and environmental input so as to drive the dynamic execution of the apps; and we introduce Android specific reduction techniques to help alleviate the state space explosion. DroidPF focuses on common security vulnerabilities in Android apps including sensitive data leakage involving a non-trivial flow- and context-sensitive taint-style analysis. DroidPF has been evaluated with 131 apps, which include real-world apps, third-party libraries, malware samples and benchmarks for evaluating app analysis techniques like ours. DroidPF precisely identifies nearly all of the previously known security issues and nine previously unreported vulnerabilities/bugs.

Mammals in northern regions chronically suffer from low temperatures during autumn-winter seasons. The aim of this study was to investigate the response of intestinal amino acid transport and the amino acid pool in muscle to chronic cold exposure via Min pig models (cold adaptation) and Yorkshire pig models (non-cold adaptation). Furthermore, this study explored the beneficial effects of glucose supplementation on small intestinal amino acid transport and amino acid pool in muscle of cold-exposed Yorkshire pigs. Min pigs (Exp. 1) and Yorkshire pigs (Exp. 2) were divided into a control group (17 °C, n =6) and chronic cold exposure group (7 °C, n =6), respectively. Twelve Yorkshire pigs (Exp. 3) were divided into a cold control group and cold glucose supplementation group (8 °C). The results showed that chronic cold exposure inhibited peptide transporter protein 1 (PepT1) and excitatory amino acid transporter 3 (EAAT3) expression in ileal mucosa and cationic amino acid transporter-1 (CAT-1) in the jejunal mucosa of Yorkshire pigs (P< 0.05). In contrast, CAT-1, PepT1 and EAAT3 expression was enhanced in the duodenal mucosa of Min pigs (P< 0.05). Branched amino acids (BCAA) in the muscle of Yorkshire pigs were consumed by chronic cold exposure, accompanied by increased muscle RING-finger protein-1 (MuRF1) and muscle atrophy F-box (atrogin-1) expression (P< 0.05). More importantly, reduced concentrations of dystrophin were detected in the muscle of Yorkshire pigs (P< 0.05). However, glycine concentration in the muscle of Min pigs was raised (P< 0.05). In the absence of interaction between chronic cold exposure and glucose supplementation, glucose supplementation improved CAT-1 expression in the jejunal mucosa and PepT1 expression in the ileal mucosa of cold-exposed Yorkshire pigs (P< 0.05). It also improved BCAA and inhibited MuRF1 and atrogin-1 expression in muscle (P< 0.05). Moreover, dystrophin concentration was improved by glucose supplementation (P< 0.05). In summary, chronic cold exposure inhibits amino acid absorption in the small intestine, depletes BCAA and promotes protein degradation in muscle. Glucose supplementation ameliorates the negative effects of chronic cold exposure on amino acid transport and the amino acid pool in muscle.