Dirofilaria immitis(Leidy, 1856) and Dirofilaria repens(Railliet & Henry, 1911) are mosquito-borne filarial nematodes that primarily affect dogs, causing heartworm disease and subcutaneous dirofilariosis. The canine heartworm is reported in different provinces in Turkey. However, studies about the transmitting mosquito species are limited. Hence, this study aimed to investigate potential vectors of D. immitisand D. repensin Aras Valley, Turkey. In total, 17,995 female mosquitoes were collected from eight villages during three mosquito seasons (2012–2014) in Aras Valley, located in north-eastern Turkey. A total of 1,054 DNA pools (527 abdomen and 527 head-thorax) were tested with Dirofilariaprimers by multiplex-polymerase chain reaction (PCR). Aedes caspiuswas the most abundant species in collection sites with 90%; this was followed by Culex theileriTheobald, 1903 (Diptera: Culicidae) (7.31%), Anopheles maculipennisMeigen 1818 (Diptera: Culicidae) (1.28%), Culex pipiensLinnaeus, 1758 (Diptera: Culicidae) (0.43%), (Anopheles) hyrcanus(Pallas, 1771) (Diptera: Culicidae) (0.37%), Aedes vexans(Meigen, 1830) (Diptera: Culicidae) (0.25%), and Culiseta annulataSchrank, 1776 (Diptera:Culicidae) (0.02%). Dirofilaria immitisand D. repenswere detected in mosquito pools from five villages. The total Dirofilariaspp. estimated infection rate was 1.33%. The highest estimated infection rate was found in Ae. vexans(6.66%) and the lowest was in Ae. caspius(1.26%). The results show that An. maculipennissl, Ae. caspius, Ae. vexans, Cx. theileriand Cx. pipiensare potential vectors of D. immitisand D. repenswith DNA in head-thorax pools; An. hyrcanusis also a likely vector, but DirofilariaDNA was found only in abdomen pools for the study area. This study revealed new potential vector species for D. immitis. Mosquitoes with natural infections of D. repenswere reported for the first time in Turkey.Graphical Abstract