55 results on '"Allard, S."'
Search Results
2. Modulation in vitro et in vivo de la signalisation calcique pour décrypter les mécanismes moléculaires responsables du développement de l’hyperaldostéronisme primaire
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Fedlaoui, B., Cosentino, T., Al Sayed, Z., Giscos-Douriez, I., Hulot, J.S., Magnus, C., Sternson, S.M., Travers-Allard, S., Baron, S., Fernandes-Rosa, F.L., Zennaro, M.C., and Boulkroun, S.
- Abstract
L’hyperaldostéronisme primaire (HAP) est la forme la plus fréquente d’hypertension artérielle secondaire. Des progrès majeurs ont été réalisés dans notre compréhension des bases génétiques de l’HAP avec l’identification de mutations germinales ou somatiques dans des canaux et pompes ioniques. Ces mutations stimulent la signalisation calcique, principal régulateur de la biosynthèse d’aldostérone.
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- 2023
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3. Comment on "Prophylactic Negative Pressure Wound Therapy for Closed Laparotomy Incisions".
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Timmer, Allard S., Zwanenburg, Pieter R., and Boermeester, Marja A.
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- 2021
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4. A Complex Interrelationship between Temperature-Dependent Polyquaterthiophene (PQT) Structural and Electrical Properties
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Grigorian, S., Escoubas, S., Ksenzov, D., Duche, D., Aliouat, M., Simon, J.-J., Bat-Erdene, B., Allard, S., Scherf, U., Pietsch, U., and Thomas, O.
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The influence of annealing temperature on the structural and electrical properties of conjugated poly(dodecyl-quaterthiophene) (PQT-12) polymer films is exploited. The temperature induced changes of structural parameters are monitored by in situ grazing incident X-ray diffraction (GIXD) and the conductivity. They are complemented by studies of the dielectric properties using variable angle spectroscopic ellipsometry (VASE). An increase of the scattered intensity, the size of the crystalline domains, and the current response is observed for a first thermal cycle with stepwise heating up to 90 °C, which revealed two polymorphs with different degrees of interdigitation in PQT-12. Irreversible changes are observed for the second cycle with a higher thermal budget up to 140 °C and are connected with a transition from the highly ordered to powder-like disordered phase for the main PQT-12 form whereas the second polymorph with stronger interdigitation completely vanished. In agreement with these observations high-temperature VASE studies demonstrated a blue shift of the transitions with a reduction in the conjugation length caused by an increase in the twist and torsion of the backbone. Combined GIXD, VASE, and electrical characterizations show that PQT-12 exhibits a complex interplay between two polymorphs with a strong influence on the charge carrier transport depending on the thermal budget employed.
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- 2017
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5. 619 Preventive effect of a dermocosmetic product containing an active ingredient complex, Cicahyalumide® and Rhealba® Oat plantlets extract, on stretch mark appearance
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Mias-Vigouroux, C., Satge, C., Rouvière, C., Allard, S., Carballido, F., Bessou-Touya, S., and Duplan, H.
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- 2022
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6. Highly Luminescent Colloidal CdS Quantum Dots with Efficient Near-Infrared Electroluminescence in Light-Emitting Diodes
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Bansal, A. K., Antolini, F., Zhang, S., Stroea, L., Ortolani, L., Lanzi, M., Serra, E., Allard, S., Scherf, U., and Samuel, I. D. W.
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Quantum dots are of growing interest as emissive materials in light-emitting devices. Here first we report the formation of highly luminescent organic capped colloidal cadmium sulfide (CdS) nanoparticles having the highest photoluminescence quantum yield of 69% in solutions and 34% in neat thin films in the near-infrared range. Second, we also show efficient electroluminescence in the near-infrared from solution processed hybrid light emitting diodes (LEDs) based on such colloidal CdS quantum dots embedded in an organic semiconductor matrix forming a nanocomposite active layer. We also discuss the device structure and role of the doped active layer in efficiency improvement. With optimized active layer thickness and concentration of QDs, the device exhibits an external electroluminescence quantum efficiency of 0.62% at a peak emission wavelength of 760 nm, providing a route to solution processable flexible light sources for biosensors and medicine.
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- 2016
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7. Comment on “Prophylactic Negative Pressure Wound Therapy for Closed Laparotomy Incisions”
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Timmer, Allard S., Zwanenburg, Pieter R., and Boermeester, Marja A.
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- 2021
- Full Text
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8. Oxidation of Manganese(II) during Chlorination: Role of Bromide.
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Allard, S., Fouche, L., Dick, J., Heitz, A., and von Gunten, U.
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- 2013
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9. Prevalence of maternal anaemia and its predictors: a multi-centre study.
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Barroso, F., Allard, S., Kahan, B. C., Connotly, C., Smethurst, H., Choo, I., Khan, K., and Stanworth, S.
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- 2012
10. Literature Review of Passenger Lymphocyte Syndrome Following Renal Transplantation and Two Case Reports
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Nadarajah, L., Ashman, N., Thuraisingham, R., Barber, C., Allard, S., and Green, L.
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The authors review the literature and discuss two case reports in passenger lymphocyte syndrome in renal transplantation.
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- 2013
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11. Literature Review of Passenger Lymphocyte Syndrome Following Renal Transplantation and Two Case Reports
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Nadarajah, L., Ashman, N., Thuraisingham, R., Barber, C., Allard, S., and Green, L.
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Passenger lymphocyte syndrome (PLS) is an immune-mediated hemolysis. It occurs following ABO blood group mismatched solid organ and/or bone marrow transplantation between donor and recipient. We report two cases of PLS occurring after renal transplantation. Both recipients received live related kidney transplants; one from his mother and the other from his brother. The direction of blood group transfer, from donor to recipient, was O Rh D+ to A Rh D+ in both cases. Approximately 12 days after transplantation, both recipients showed a rapid fall in their hemoglobin levels with no identifiable bleeding source. DAT positive hemolysis was confirmed and anti-A antibodies were detected in recipient sera, confirming a diagnosis of PLS. Both cases required blood transfusion support to maintain their hemoglobin and both had good renal outcomes. We have identified 99 PLS cases following renal transplant in the English literature. Previous ABO sensitization, donor blood group O to recipient blood group A or B transfer, and ciclosporin treatment have been identified as risk factors for PLS. Clinical outcomes in general are good; nonetheless, cases of graft failure and deaths have been reported. Early diagnosis and appropriate treatment are important in at risk individuals.
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- 2013
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12. The incidence and magnitude of fibrinolytic activation in trauma patients
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RAZA, I., DAVENPORT, R., ROURKE, C., PLATTON, S., MANSON, J., SPOORS, C., KHAN, S., De'ATH, H.D., ALLARD, S., HART, D.P., PASI, K.J., HUNT, B.J., STANWORTH, S., MacCALLUM, P.K., and BROHI, K.
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Summary.Background:Trauma is a global disease, with over 2.5 million deaths annually from hemorrhage and coagulopathy. Overt hyperfibrinolysis is rare in trauma, and is associated with massive fatal injuries. Paradoxically, clinical trials suggest a much broader indication for antifibrinolytics.
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- 2013
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13. Dependency of BET surface area on particle size for some granitic minerals
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Dubois, I. E., Holgersson, S., Allard, S., and Malmström, M. E.
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In order to assess the geochemical retention properties of rocks, which will be the final barrier for radionuclide transport to the biosphere in the case of a failed deep underground repository for spent nuclear fuel, radionuclide sorption experiments are usually made with crushed material. This raises the issue of extrapolating results obtained from laboratory experiments to the field scale. As sorption is generally related to the surface area of the geological material, it is then important to consider the dependency of the specific surface area on the particle size. In this work, BET surface area determinations of samples of different particle sizes are conducted on two minerals commonly found in granite: labradorite and magnetite. The results show a linear relationship between BET surface area and the inverse of the particle size, up to a certain particle size. Furthermore, results also show that the specific surface area for intact, larger pieces is much smaller than the one predicted by a linear extrapolation of results on crushed material. Therefore, extrapolation of BET area for fine particles to the field situation will lead to an overestimation of the surface area and thereby also the radionuclide sorption, if sorption coefficients are extrapolated as well. Also of importance is that these results show that sorption experiments on crushed material may dominantly reflect properties of new surface, created during the mechanically treatment of the samples.
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- 2011
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14. Transgenic Mice as a Model of Pre-Clinical Alzheimer's Disease
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T. Ferretti, M., Partridge, V., C. Leon, W., Canneva, F., Allard, S., N. Arvanitis, D., Vercauteren, F., Houle, D., Ducatenzeiler, A., L. Klein, W., G. Glabe, C., Szyf, M., and C. Cuello, A.
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At diagnosis, Alzheimer's disease (AD) brains are extensively burdened with plaques and tangles and display a degree of synaptic failure most likely beyond therapeutic treatment. It is therefore crucial to identify early pathological events in the progression of the disease. While it is not currently feasible to identify and study early, pre-clinical stages of AD, transgenic (Tg) models offer a valuable tool in this regard. Here we investigated cognitive, structural and biochemical CNS alterations occurring in our newly developed McGill-Thyl-APP Tg mice (over-expressing the human amyloid precursor protein with the Swedish and Indiana mutations) prior to extracellular plaque deposition. Pre-plaque, 3-month old Tg mice already displayed cognitive deficits concomitant with reorganization of cortical cholinergic pre-synaptic terminals. Conformational specific antibodies revealed the early appearance of intracellular amyloid β (Aβ)-oligomers and fibrillar oligomers in pyramidal neurons of cerebral cortex and hippocampus. At the same age, the cortical levels of insulin degrading enzyme -a well established Aβ-peptidase, were found to be significantly down-regulated. Our results suggest that, in the McGill-Thy1-APP Tg model, functional, structural and biochemical alterations are already present in the CNS at early, pre-plaque stages of the pathology. Accumulation of intraneuronal neurotoxic Aβ-oligomers (possibly caused by a failure in the clearance machinery) is likely to be the culprit of such early, pre-plaque pathology. Similar neuronal alterations might occur prior to clinical diagnosis in AD, during a yet undefined ‘latent’ stage. A better understanding of such pre-clinical AD might yield novel therapeutic targets and or diagnostic tools.
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- 2011
15. Transgenic Mice as a Model of Pre-Clinical Alzheimers Disease
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T. Ferretti, M., Partridge, V., C. Leon, W., Canneva, F., Allard, S., N. Arvanitis, D., Vercauteren, F., Houle, D., Ducatenzeiler, A., L. Klein, W., G. Glabe, C., Szyf, M., and C. Cuello, A.
- Abstract
At diagnosis, Alzheimers disease (AD) brains are extensively burdened with plaques and tangles and display a degree of synaptic failure most likely beyond therapeutic treatment. It is therefore crucial to identify early pathological events in the progression of the disease. While it is not currently feasible to identify and study early, pre-clinical stages of AD, transgenic (Tg) models offer a valuable tool in this regard. Here we investigated cognitive, structural and biochemical CNS alterations occurring in our newly developed McGill-Thyl-APP Tg mice (over-expressing the human amyloid precursor protein with the Swedish and Indiana mutations) prior to extracellular plaque deposition. Pre-plaque, 3-month old Tg mice already displayed cognitive deficits concomitant with reorganization of cortical cholinergic pre-synaptic terminals. Conformational specific antibodies revealed the early appearance of intracellular amyloid (A)-oligomers and fibrillar oligomers in pyramidal neurons of cerebral cortex and hippocampus. At the same age, the cortical levels of insulin degrading enzyme -a well established A-peptidase, were found to be significantly down-regulated. Our results suggest that, in the McGill-Thy1-APP Tg model, functional, structural and biochemical alterations are already present in the CNS at early, pre-plaque stages of the pathology. Accumulation of intraneuronal neurotoxic A-oligomers (possibly caused by a failure in the clearance machinery) is likely to be the culprit of such early, pre-plaque pathology. Similar neuronal alterations might occur prior to clinical diagnosis in AD, during a yet undefined ‘latent’ stage. A better understanding of such pre-clinical AD might yield novel therapeutic targets and or diagnostic tools.
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- 2011
16. Definition and drivers of acute traumatic coagulopathy: clinical and experimental investigations
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FRITH, D., GOSLINGS, J.C., GAARDER, C., MAEGELE, M., COHEN, M.J., ALLARD, S., JOHANSSON, P.I., STANWORTH, S., THIEMERMANN, C., and BROHI, K.
- Abstract
Background: Acute traumatic coagulopathy (ATC) is an impairment of hemostasis that occurs early after injury and is associated with a 4‐fold higher mortality, increased transfusion requirements and organ failure. Objectives: The purpose of the present study was to develop a clinically relevant definition of ATC and understand the etiology of this endogenous coagulopathy. Patients/methods: We conducted a retrospective cohort study of trauma patients admitted to five international trauma centers and corroborated our findings in a novel rat model of ATC. Coagulation status on emergency department arrival was correlated with trauma and shock severity, mortality and transfusion requirements. 3646 complete records were available for analysis. Results: Patients arriving with a prothrombin time ratio (PTr) > 1.2 had significantly higher mortality and transfusion requirements than patients with a normal PTr (mortality: 22.7% vs. 7.0%; P< 0.001. Packed red blood cells: 3.5 vs. 1.2 units; P< 0.001. Fresh frozen plasma: 2.1 vs. 0.8 units; P< 0.001). The severity of ATC correlated strongly with the combined degree of injury and shock. The rat model controlled for exogenously induced coagulopathy and mirrored the clinical findings. Significant coagulopathy developed only in animals subjected to both trauma and hemorrhagic shock (PTr: 1.30. APTTr: 1.36; both P< 0.001 compared with sham controls). Conclusions: ATC develops endogenously in response to a combination of tissue damage and shock. It is associated with increased mortality and transfusion requirements in a dose‐dependent manner. When defined by standard clotting times, a PTr > 1.2 should be adopted as a clinically relevant definition of ATC.
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- 2010
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17. Five‐year followup of a cognitive–behavioral intervention for patients with recently‐diagnosed rheumatoid arthritis: Effects on health care utilizationClinicalTrials.gov identifier: NTC001214.
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Sharpe, L., Allard, S., and Sensky, T.
- Abstract
To investigate whether cognitive–behavioral therapy (CBT) administered early in the course of rheumatoid arthritis (RA) has long‐term effects on health care use.We reviewed the files of 47 of the original 53 patients with early RA who volunteered for a randomized controlled trial comparing CBT with no psychological intervention. Occasions of service provision associated with RA were documented and health care use was compared between groups.The CBT group used fewer health care resources than the control group in the 5 years following intervention. Significant differences were observed for the number of inpatient nights, physiotherapy referrals, injections, and for total health care use. There was a trend that closely approached significance toward fewer episodes of surgery and orthopedic referrals in the CBT group.These results suggest that CBT administered early in the course of RA can reduce health care use for the first 5 years after treatment. This is a stringent test of the efficacy of a brief psychological intervention, and supports the fact that brief psychological treatments can have long‐term effects.
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- 2008
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18. Complexing Properties of α-Isosaccharinate: Stability Constants, Enthalpies and Entropies of Th-complexation with Uncertainty Analysis
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Allard, S. and Ekberg, C.
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- 2006
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19. The Maize An2 Gene is Induced by Fusarium Attack and Encodes an ent-Copalyl Diphosphate Synthase
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Harris, L., Saparno, A., Johnston, A., Prisic, S., Xu, M., Allard, S., Kathiresan, A., Ouellet, T., and Peters, R.
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Abstract: Using the technique of differential display, a maize transcript was identified whose silk tissue expression is induced in the presence of the ear rot pathogen Fusarium graminearum. The 3445 nt transcript includes a 727 nt 5′ untranslated leader with the potential for extensive secondary structure and represents the maize gene An2. An2 encodes a copalyl diphosphate synthase (CPS)-like protein with 60% amino acid sequence identity with the maize An1 gene product involved in gibberellin (GA) biosynthesis. Recombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent-copalyl diphosphate (ent-CPP) synthases (ent-CPS). Notably, the presence of an additional ent-CPS gene is consistent with previous reports that maize GA biosynthesis can proceed in the absence of An1. In addition, northern blot analysis showed that An2 transcript levels were strongly up-regulated by Fusarium attack, with an increase in silk, husk and ear tip tissues as early as 6 h after inoculation of silk channels with spore suspensions of various Fusarium sp. Gene expression of a third maize CPS-like gene, Cpsl1, is not affected by Fusarium infection. The Fusarium-inducible nature of An2 is also consistent with a previous report that cell-free extracts from maize seedlings produce ent-CPP derived diterpenes in response to Fusarium infection. However, it is not known whether An2 is involved in defense-related secondary metabolism in addition to GA synthesis.
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- 2005
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20. A structural perspective on the enzymes that convert dTDP-d-glucose into dTDP-l-rhamnose
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Dong, C., Beis, K., Giraud, M.-F., Blankenfeldt, W., Allard, S., Major, L.L., Kerr, I.D., Whitfield, C., and Naismith, J.H.
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Bacteria have a rich collection of biochemical pathways for the synthesis of complex metabolites. These conversions often involve chemical reactions that are hard to reproduce in the laboratory. An area of considerable interest is in the manipulation and synthesis of carbohydrates. In contrast with amino acids, carbohydrates are densely functionalized (each carbon atom is attached to at least one heteroatom) and this holds out the prospect of discovering novel enzyme mechanisms. The results from the study of the biosynthetic dTDP-l-rhamnose pathway are discussed. dTDP-l-rhamnose is a key intermediate in many pathogenic bacteria, as it is the donor for l-rhamnose, which is found in the cell wall of important human pathogens, such as Mycobacteria tuberculosis and Salmonella typhimurium. All four enzymes have been structurally characterized; in particular, the acquisition of structural data on substrate complexes was extremely useful. The structural data have guided site-directed-mutagenesis studies that have been used to test mechanistic hypotheses. The results shed light on three classes of enzyme mechanism: nucleotide condensation, short-chain dehydrogenase activity and epimerization.
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- 2003
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21. Long‐term efficacy of a cognitive behavioural treatment from a randomized controlled trial for patients recently diagnosed with rheumatoid arthritis
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Sharpe, L., Sensky, T., Timberlake, N., Ryan, B., and Allard, S.
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Objectives. This study examined the long‐term efficacy of a cognitive behavioural intervention for patients with recent‐onset, seropositive rheumatoid arthritis (RA).Methods. Fifty‐three consecutive patients with less than a 2‐yr history of classic or definite RA were recruited into the trial. All participants received routine medical management during the study, and half were randomly allocated to receive an 8‐week adjunctive psychological intervention. All assessments were conducted blind to the allocation. This paper reports intention‐to‐treat analyses of the 18‐month follow‐up.Results. Consistent with short‐term results, significant differences were found between the groups in depressive symptoms. The intervention group maintained improvements in joint function, although those in routine care made similar improvements over the ensuing 18 months. At follow‐up, group differences emerged for disability and anxiety.Conclusions. These results indicate that cognitive behavioural intervention offered as an adjunct to standard clinical management early in the course of RA is efficacious in producing improvements in both psychological and physical indices. Furthermore, improvements appear to increase 18 months after a brief, time‐limited psychological treatment.
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- 2003
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22. Characteristics of handicap for patients with recent onset rheumatoid arthritis: the validity of the Disease Repercussion Profile
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Sharpe, L., Sensky, T., Brewin, C.R., and Allard, S.
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Objectives. To investigate the applicability of the Disease Repercussion Profile (DRP) in the assessment of people with recently diagnosed rheumatoid arthritis. Previous research using this instrument has been confined to chronic samples.Methods. Fifty-three patients with recent onset rheumatoid arthritis completed the DRP and other commonly used clinical outcome measures.Results. The life areas of the DRP were highly interrelated, with the exception of finance. The total DRP score was associated with joint function, disability, subjective pain and coping, but was most highly associated with emotional disturbance, particularly depressive symptoms. No associations were found between measures of disease or demographic variables and DRP subscales. Activity was the area most often affected, with social life, emotions and appearance all more strongly endorsed than finances and relationships. However, whenever any of the areas was endorsed as affected, its impact was inevitably rated as very important. The pattern of self-perceived handicap was different from that reported in people with chronic arthritis.Conclusions. These results offer evidence that the DRP provides a valid measure of handicap for patients with early illness even with relatively low levels of disability. However, handicap in early rheumatoid arthritis may be more highly associated with psychological distress than in later stages of the illness.
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- 2001
23. The course of depression in recent onset rheumatoid arthritis
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Sharpe, L., Sensky, T., and Allard, S.
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- 2001
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24. A blind, randomized, controlled trial of cognitive-behavioural intervention for patients with recent onset rheumatoid arthritis: preventing psychological and physical morbidity
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Sharpe, L., Sensky, T., Timberlake, N., Ryan, B., Brewin, C. R., and Allard, S.
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- 2001
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25. A model for understanding and affecting cancer genetics information seeking
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Johnson, J. D., Andrews, J. E., and Allard, S.
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- 2001
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26. Molecular mechanisms of hormone-mediated Müllerian duct regression: involvement of beta-catenin.
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Allard, S, Adin, P, Gouédard, L, di Clemente, N, Josso, N, Orgebin-Crist, M C, Picard, J Y, and Xavier, F
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Regression of the Müllerian duct in the male embryo is one unequivocal effect of anti-Müllerian hormone, a glycoprotein secreted by the Sertoli cells of the testis. This hormone induces ductal epithelial regression through a paracrine mechanism originating in periductal mesenchyme. To probe the mechanisms of action of anti-Müllerian hormone, we have studied the sequence of cellular and molecular events involved in duct regression. Studies were performed in male rat embryos and in transgenic mice overexpressing or lacking anti-Müllerian hormone, both in vivo and in vitro. Anti-Müllerian hormone causes regression of the cranial part of the Müllerian duct whereas it continues to grow caudally. Our work shows that this pattern of regression is correlated with a cranial to caudal gradient of anti-Müllerian hormone receptor protein, followed by a wave of apoptosis spreading along the Müllerian duct as its progresses caudally. Apoptosis is also induced by AMH in female Müllerian duct in vitro. Furthermore, apoptotic indexes are increased in Müllerian epithelium of transgenic mice of both sexes overexpressing the human anti-Müllerian hormone gene, exhibiting a positive correlation with serum hormone concentration. Inversely, apoptosis is reduced in male anti-Müllerian hormone-deficient mice. We also show that apoptosis is a decisive but not sufficient process, and that epitheliomesenchymal transformation is an important event of Müllerian regression. The most striking result of this study is that anti-Müllerian hormone action in peri-Müllerian mesenchyme leads in vivo and in vitro to an accumulation of cytoplasmic beta-catenin. The co-localization of beta-catenin with lymphoid enhancer factor 1 in the nucleus of peri-Müllerian mesenchymal cells, demonstrated in primary culture, suggests that overexpressed beta-catenin in association with lymphoid enhancer factor 1 may alter transcription of target genes and may lead to changes in mesenchymal gene expression and cell fate during Müllerian duct regression. To our knowledge, this is the first report that beta-catenin, known for its role in Wnt signaling, may mediate anti-Müllerian hormone action.
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- 2000
27. Biochemical and Genetic Characterization of the Flagellar Filaments from the Rumen Anaerobe Butyrivibrio fibrisolvensOR77
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Kalmokoff, M.L., Allard, S., Austin, J.W., Whitford, M.F., Hefford, M.A., and Teather, R.M.
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Flagellar filaments isolated from stationary phase cells of the rumen anaerobe Butyrivibrio fibrisolvensOR77 were composed of three flagellins (54, 62 and 68kDa). All three proteins demonstrated a conserved N-terminus, with homology to the N-terminus of the flagellin from the intestinal anaerobe Roseburia cecicola. Two genes encoding flagellins were identified and cloned from the genome of this isolate. Transcriptional analysis indicated that both genes were expressed, but as separate unit length messages. The first gene (flaA) encoded a flagellin with a predicted molecular weight of 50 kDa. Transcription initiation within the flaAoperon occured at two different promoter sites. The first promoter demonstrated significant sequence similarity to the flagellum specific promoter consensus sequence (σ28), the second to the general “housekeeping” consensus sequence (σ43). The second flagellin gene (flaB) encoded a flagellin with a predicted molecular weight of 54 kDa. Differences in the predicted and determined flagellin molecular weights, the number of flagellins and corresponding genes, and a positive reaction using an immunoassay for the detection of digoxigenin labeled sugars, indicate that the flagellins of B. fibrisolvensOR77 undergo post-translational modification in the form of glycosylation.
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- 2000
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28. Microemulsion formulation of cyclosporin (Sandimmun Neoral<SUP>®</SUP>) vs Sandimmun<SUP>®</SUP>: comparative safety, tolerability and efficacy in severe active rheumatoid arthritis
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Krüger, K., Trigg, L.B., Vaz, A. Lopes, Whatmough, I., Manger, B., A.-G., Schmidt, Mur, E., Nygaard, H., Weiner, S.M., Rainer, F., Sack, M.R., Schiff, M.H., Schnitzer, T.J., Allard, S., Cohen, S.B., Emery, P., Flipo, R.M., Goobar, J., Jayawardena, S., Job-Deslandre, C., Jubb, R.W., and Yocum, D.E.
- Abstract
Objective. To compare the safety, tolerability and efficacy of the new oral microemulsion formulation of cyclosporin A (CyA; Sandimmun Neoral®) and the original CyA formulation (Sandimmun®), in patients with severe active rheumatoid arthritis (RA), over a 12-month period.Methods. In this double-blind, multicentre study, patients were randomized to treatment with Neoral or Sandimmun, starting with 2.5 mg/kg/day, with dose adjustments after 4 weeks. Primary efficacy criteria included patients' assessment of disease activity. Pharmacokinetic and safety assessments were performed at regular intervals.Results. Compared with Sandimmun, Neoral showed a consistent trend towards greater clinical efficacy from week 12 onwards, including a significant difference in patients' assessment of disease activity at the study end-points. A significantly lower increase in dose from baseline was observed with Neoral at week 24. Pharmacokinetic assessments at week 24 showed increased absorption and decreased variability with Neoral. No differences in safety were found between treatment groups.Conclusion. These observations indicate that Neoral is as safe and at least as effective as Sandimmun and have important implications for patient management given the increasing role for CyA in the treatment of severe, active RA.
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- 2000
29. 35th Annual Meeting of the European Association for the Study of Diabetes
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Melander, A., Olsson, J., Lindberg, G., Salzman, A., Howard, T., Stang, P., Lydick, E., Emslie-Smith, A., Boyle, D. I. R., Evans, J. M. M., Macdonald, T. M., Bain, J., Sullivan, F., Juhl, C., Pørksen, N., Sturis, J., Hollingdal, M., Pincus, S., Veldhuis, J., Dejgaard, A., Schmitz, O., Kristensen, J. S., Frandsen, K. B., Bayer, Th., Müller, P., Dunning, B. E., Paladini, S., Gutierrez, C., Deacon, R., Valentin, M., Grunberger, G., Weston, W. M., Patwardhan, R., Rappaport, E. B., Sargeant, L. A., Wareham, N. J., Khaw, K. T., Zethelius, Björn, Lithell, Hans, Hales, C. Nicholas, Berne, Christian, Lakka, H.-M., Oksanen, L., Tuomainen, T.-P., Kontula, K., Salonen, J. T., Dekker, J. M., de Boks, P., de Vegt, F., Stehouwer, C. D. A., Nijpels, G., Bouter, L. M., Heine, R. J., Bruno, G., Cavallo-Perin, P., Bargero, G., D’Errico, N., Borra, M., Macchia, G., Pagano, G., Newton, R. W., Ruta, D. A., New, J. P., Wallace, C., Roxburgh, M. A., Young, R. J., Vaughan, N. J. A., Elliott, P., Brennan, G., Devers, M., MacAlpine, R., Steinke, D., Lawson, D. H., Decallonne, B., Casteels, K., Gysemans, C., Bouillon, R., Mathieu, C., Linn, Thomas, Strate, Christine, Schneider, Kerstin, Funda, D. P., Jirsa, M., Kozáková, H., Kaas, A., Kofronová, O., Tlaskalová-Hogenová, H., Buschard, K., Wanka, H., Hartmann, A., Kuttler, B., Rasmussen, S. B., Sørensen, T. S., Markholst, H., Petersen, J. S., Karounos, D., Dyrberg, T., Mabley, J. G., Haskó, G., Szabó, C., Seissler, J., Nguyen, T. B. T., Steinbrenner, H., Scherbaum, W. A., Cipriani, R., Gabriele, A., Sensi, M., Guidobaldi, L., Pantellini, F., Cerrito, M. G., Scarpa, S., Di Mario, U., Morano, S., Ceolotto, G., Iori, E., Baritono, E., Del Prato, S., Semplicini, A., Trevisan, R., Zerbini, G., Meregalli, G., Asnaghi, V., Tentori, F., Maestroni, A., Mangili, R., Marescotti, C., Vedovato, M., Tiengo, A., Tadjieva, J., Mankovsky, B. N., Van Aken, S., Raes, A., Vande Walle, J., Matthys, D., Craen, M., Hansen, H. 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C., Kaya, F., Süsleyici, B., Öztürk, M., Eisner, M., Guldbakke, B., Karpenko, N., Brizgalova, G., Alesina, M., Røder, M. E., Schwartz, R. S., Prigeon, R., Kahn, S. E., Kendereški, A., Micić, D., Šumarac, M., Macut, Dj., Zonć, S., Čolić, M., Cvijović, G., Gligorović, P., Courtney, C. H., Atkinson, A. B., Ennis, C., Sheridan, B., Bell, P. M., Jolly, M., Amin, R., Godsland, I., Horvoka, R., Anyaoku, V., Lawrence, N., Krasova, N., Sergienko, L., Mingrone, G., Plat, L., Balasse, E. O., Zykova, T., Jenssen, T., Strelkova, A., Zykova, S., Tipisova, E., Féry, F., Wijenaike, A. N., Watt, P. W., Jung, R. T., Bolton-Smith, C., Rennie, M. J., Ludvik, B., Aigmueller, Th., Waldhaeusl, W., Courtois, P., Bource, F., Guenat, E., Philippe, J., Jéquier, E., Tappy, L., Benny, Santosa, Grönemeyer, Dietrich, Aygen, Sitke, Scholz, Nicole, Busch, Martin, Tauveron, I., Rochon, C., Dejax, C., Benoit, P., Capitan, P., Bayle, G., Prugnaud, J., Fabricio, A., Champredon, C., Thieblot, P., Grizard, J., Nielsen, M. F., Nyholm, B., Chandramouli, V., Schumann, W. C., Landau, B. R., Rizza, R. A., Mitrakou, A., Meyer, C., Tolias, A., Platanisiotis, D., Vlachos, L., Gerich, J., Wajngot, A., Sprangers, F., Jellema, W. T., Lopuhaä, C. E., van Lieshout, J. J., van der Zee, J. S., Mithieux, G., Croset, M., Zitoun, C., Hurot, J. M., Rajas, F., Montano, S., Willem, R., Verbruggen, I., Grue-Sørensen, G., Björkling, F., Watson, N. D., Burns, S. P., Murphy, H. C., Iles, R. A., Cohen, R. D., Rooney, K., Swan, V., Phuyal, J., Millar, J., Bryson, J., Denyer, G., Caterson, I., Thompson, C., Gaster, M., Handberg, Aa., Schrøder, H. D., Alzaid, A., Sobki, S., Thye-Rønn, P., Alford, F., Christopher, M., Gras, F., Brunmair, B., Neschen, S., Py, G., Lambert, K., Raynaud, E., Mercier, J., Tsuchihashi, K., Sumida, Y., Fujimoto, H., Nakamura, M., Miyata, E., Furuta, M., Katsuki, A., Ito, K., Sasaki, R., Hori, Y., Yano, Y., Adachi, Y., Lauritz, J., Eriksson, J. W., Burén, J., Zhao, L. J., Li, Z.-C., Kullin, M., Karlsson, F. A., Redondo, A., Puente, J., Clemente, F., González, N., Moberg, E., Amer, P., Hagström-Toft, E., Bolinder, J., Björnholm, M., Krook, A., Galuska, D., Myers, M., Zierath, J. R., Wallberg-Henriksson, H., Niklasson, M., Strindberg, L., Sternberg, F., Hebeda, S., Kratzer, W., Salgado, M. I., Hoss, U., Kalatz, B., Lohmann, S., Fussgänger, R., Khomazjuk, A. I., Ncscheret, A. P., Gonchar, I. V., Quinones-Galvan, A., Sironi, A. M., Cominacini, L., Nagai, Y., Yamashita, H., Takamura, T., Kobayashi, K., Szanto, I., Peth, J. A., Kinnick, T. R., Youngblood, E. B., Tritschler, H. J., Henriksen, E. J., Gašperíková, D., Rufo, C., Teran-Garcia, M., Nakamura, M. T., Clarke, S. D., Pye, S., Zhang, Z., Radziuk, J., Guignot, L., Bell, K. S., Lim-Fraser, M., Cooney, G., Kraegen, E. W., Takayama, S., Legare, D. J., Macedo, M. P., Lautt, W. W., Bradley, B., Barron, P., Davies, J., Ader, M., Richey, J. M., Ait El Mkadem, S., Macari, F., Renard, E., Méchaly, I., Brun, J. F., Cros, G., Bringer, J., del Aguila, L. F., Krishnan, R. K., Farrell, P. A., Ulbrecht, J., Correll, P. H., Kirwan, J. P., Mei, J., Rahn-Landström, T., Brindley, D., Manganiello, V., Degerman, E., Ziv, E., Shafrir, E., Kaiman, R., Galer, S., Bar-On, H., Gerő, L., Földes, K., Janssen, J., Járay, J., Perner, F., Haap, M., Houdali, B., Schmit, M. B., Dietze, G. J., Perrini, S., Natalicchio, A., Montrone, C., de Robertis, O., De Pergola, G., Strack, V., Kellerer, M., Kausch, C., Condorelli, G., Beguinot, F., Häring, H.-U., Song, X. M., Chibalin, A. V., Ryder, J. W., Jiang, X. J., Alessi, D. R., Hennige, A. M., Metzinger, E., Seipke, G., Trüb, T., Hey, A., Sørensen, A. R., Schäffer, L., Drejer, K., Kurtzhals, P., Hansen, B. F., Matozaki, T., Noguchi, T., Yamao, T., Takada, T., Ochi, F., Takeda, H., Inagaki, K., Hosoka, T., Kasuga, M., Schürt, M., Meier, M., Drenckhan, M., Meyer, M., Aries, S. P., Klein, H. H., Telting, D., van der Zon, G. C. M., Dorrestijn, J., Maassen, J. A., Clapham, J. C., Holder, J. C., Tomlinson, K. M., Pickavance, L., Buckingham, R., Wilding, J., Jacinto, S. M., Harrold, J., Ljung, B., Kjellstedt, A., Thalén, P., Widdowson, P., Williams, G., Oakes, N., Aoki, K., Saito, T., Satoh, S., Mukasa, K., Kaneshiro, M., Kawasaki, S., Hoshino, K., Okamura, A., Sekihara, H., Smith, U., Johansson, A., Nilsson, E., Olausson, T., Nakazawa, T., Suzuki, M., Martinez, J., Murado, P., Azal, Ö., Yönem, A., Çakır, B., Polat, Z., Kutlu, M., Çorakçı, A., Bayraktar, M., Gürlek, A., Koray, Z., Damian, M. S., Linn, T., Laube, H., Arzner, S., Meißner, H.-P., Giunti, S., Comune, M., Cassader, M., Conte, M. R., Sacchi, C., Musso, G., Mecca, F., Depetris, N., Gambino, R., Perin, P. Cavallo, Kawakami, S., Sandqvist, M., Jansson, P.-A., Šindelka, G., Widimský, J., Haas, T., Prázný, M., Mari, A., Nolan, J. J., Uusitupa, M. I. J., Karşıdağ, K., Hacıhanefioğlu, B., Dinççağ, N., Drivsholm, T., Palacios, R. T., Vølund, A., Pedersen, Oluf B., Letiexhe, M. R., Scheen, A. J., Quiñones Galvan, A., Simeoni, M., Basu, A., Uosukainen, A., Mäkimattila, S., Schlenzka, A., Adler, A. I., Levy, J., Stevens, R., Matthews, D., Holman, R., Boland, B. J., Jeanjean, M., Hermans, M. P., Maudoigt, C., Tonglet, R., Robert, A., Quiñones-Galvan, A., Cini, G., Galetta, F., Sanna, G., Gernone, F., Janssen, M. J., Gonera, R. K., Wolffenbuttel, B. H. R., de Leeuw, P. W., Schaper, N. C., Molęda, P., Kuczerowski, R., Czech, A., Tatoń, J., Taddei, S., Patiag, D., Qu, X., Wilkes, M., Gray, S., Seale, J. P., Donnelly, R., Campión, J., Maestro, B., Dávila, N., Carranza, M. C., Calle, C., Hales, C. N., Fernández-Real, J. M., Grasa, M., Pugeat, M., Barret, C., Ricart, W., Lindmark, S., Olsson, T., Tufvesson, M., Loeblein, K., Mehnert, B., Haering, H. U., Rave, Klaus, Heise, Tim, Clauson, Per, Hirschberger, Sabine, Heinemann, Lutz, Claret, M., Nadal, B., Truc, A., Rossi, L., Hildebrand, P., Ketterer, S., Beglinger, C., Keller, U., Gyr, K., Parvin, S., Overkamp, D., Vayreda, M., González-Huix, F., G-Huix, F., Zavaroni, I., Gasparini, P., Massironi, P., Zuccarelli, A., Delsignore, R., Reaven, G. M., Sheu, W. H. H., Lee, W. J., Chen, Y.-T., Iraklianou, S., Tournis, S., Volonakis, I., Spylopoulou, M., Bilianou, E., Melidonis, A., Foussas, S., Güler, Serdar, çakir, Bekir, Demi̇rbaş, Berrin, Gürsoy, Gül, Serter, Rüştü, Aral, Yalçin, Morton, G., Lee, S., Fahey, R., de Silva, A., Cai, X. J., Buckingham, R. E., Arch, J. R. S., Wilson, S., Clausen, J. T., Kristensen, P., Nielsen, P. F., Wulff, B. S., Thim, L., Holness, M. J., Sugden, M. C., Fryer, L. G. D., Munns, M. J., Mannucci, E., Ognibene, A., Cremasco, F., Bardini, G., Mencucci, A., Ciani, S., Pierazzuoli, E., Tsuchihashil, K., Rigalleau, V., Delafaye, C., Baillet, L., Vergnot, V., Brunou, P., Gatta, B., Gin, H., Felber, J. P., Munger, R., Assimacopoulos, F., Bobbioni, E., Golay, A., Wilken, M., Larsen, F. S., Buckley, D., Molina, L. M., Marquez, L., Arbeo, A., Hernandez, C., Kofod, H., Damholt, A. B., Buchan, A., Márquez, L., Luque, M. A., Sarti, L., Sutton, P. J., Behle, K., Heimesaat, M. M., Hüfner, M., Gravholt, Claus Højbjerg, Mølier, Niels, Christiansen, Jens Sandahl, Schmitz, Ole, Deacon, C. F., Brock, B., Knudsen, L. B., Agersø, H., Huusfeldt, P. O., Kelly, C. M. N., Brunn, C., Schioos, J., Sewing, S., Lemansky, P., Wawro, S., Mest, H. J., Taguchi, T., Motoshima, H., Yoshizato, K., Guenifi, Amel, Henriksson, M., Johansson, J., Shafqat, J., Tally, M., Wahren, J., Jömvall, H., Ekberg, K., Rigler, R., Pramanik, A., Kratz, G., Johansson, B.-L., Uhlén, M., Jörnvall, H., Forst, T., Dufayet De La Tour, D., Kunt, T., Pfützner, A., Goitom, K., Pohlmann, T., Schneider, S., Johansson, B. L., Löbig, M., Engelbach, M., Beyer, J., Ekman, Bertil, Nyström, Fredrik, Arnqvist, Hans J., Halvatsiotis, P. G., Meek, S., Bigelow, M., Nair, K. S., Maghsoudi, S., Fisker, S., Vølund, A. A., Jörgensen, J. O. L., Christiansen, J. S., Hilsted, J., Mazerkina, N. A., Tiulpakov, A. N., Gorelyshev, S. K., Peterkova, V. A., Macut, D. J., Dieguez, C., Casanueva, F. F., Catalina, P. F., Mallo, F., Andrade, A., García-Mayor, R. V. G., Popova, V. V., ter Maaten, J. C., Popp-Snijders, C., Madsen, L., Ukropec, J., Bergene, E., Rnstan, A. C., Berge, R., Arner, P., Wahl, G., Häring, H., Bryson, J. M., Curtis, S. E., Caterson, I. D., Winzell, M. Sörhede, Svensson, H., Ahnén, B., Holm, C., Phillips, C., Madigan, C., Owens, D., Collins, P., Johnson, A., Tomkin, G. H., Cabezas, M. Castro, van Oostrom, A. J. H. H. M., Erkelens, D. W., Summers, L. K. M., Fielding, B. A., Ilic, V., Clark, M. L., Frayn, K. N., Pietzsch, J., Julius, U., Nitzsche, S., Fischer, S., Lindgren, C., Amrot-Fors, L., Hoffmann, M. M., Luft, D., Schmülling, R.-M., D’Adamo, M., Leonetti, F., Paoloni, A., Ribaudo, M. C., Basso, M. S., Elmore, U., Restuccia, A., Sbraccia, P., Emilsson, V., O’Dowd, J., Heyman, R., Cawthorne, M. A., Pelikánová, T., Kazdová, L., Žák, A., Chvojková, Š., Özer, E. M., Kadıoğlu, P., Korugan, Ü., Hatemi, H., Rivellese, A. A., Dullaart, R. P. F., Riemens, S. C., Sluiter, W. J., van Tol, A., Farnier, M., Megnien, S., Turpin, G., Stulp, B. K., Brambilla, P., Brunelli, A., Riva, M. C., Manzoni, P., de Poli, S., Riboni, S., Stolk, R. P., Meijer, R., Wink, O., Zelissen, P. M. J., van Gils, A. P. G., Grobbee, D. 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W., Taverna, M., Guerre-Millo, M., Chevalier, A., Pacher, N., Slama, G., Gorshunska, M., Buyken, A. E., Heitkamp, G., Kabir, M., Oppert, J. M., Wursch, P., Bruzzo, F., Rahman, M. H., Fatima, K., Ahmed, S., Mondal, H. N., Yilmaz, M., Öztok, U., Karakoç, A., Çakır, N., Düzgün, E., Yetkin, İ., Arslan, M., Şardaş, S., Wilding, John, Géloën, A., Baret, G., Dalmaz, Y., Peyronnet, J., Clémenceau, B., Martignat, L., Lalain, S., Gouin, E., Kenda-Ropson, N., Miller, A. O. A., You, S., Aguilera, E., Recasens, M., Flores, L., Ricart, M. J., Fernández-Cruz, L., Esmatjes, E., Crenier, L., Noël, C., Le Moine, A., Mahy, M., Danguy, A., Kiss, R., Goldman, M., Bracci, C., De Haan, B., Nilsson, K., Deschamps, J. Y., Glagoličová, A., Smrčková, I., Dieterle, C., Illner, W. D., Land, W., Feldmeier, H., Scheuer, R., Lalli, C., Di Loreto, C., Ellringmann, U., Balks, H. J., v. zur Mühlen, A., Dengler, R., Weissenborn, K., Rasmussen, B. M., Ørskov, L., Watson, J., Owen, G., Barrett, G., Ingleby, J., Weiss, M., Deary, I., Cavan, D., Kerr, D., Bruneiii, A., Cuce’, A., Elsing, H. G., Kühne, D., Quinn, N. D., Warner, D. P., Buysschaert, M., Jamal, R., O’Brien, T., Latare, P., Mullen, J., Rein, A., Wargo, M., Parkes, J. L., Ginsberg, B., Sotiropoulos, A., Peppas, Th. A., Kotsini, V., Apostolou, O., Bousboulas, S., Michailidis, E., Sawala, M., Pappas, S., Nilsson, P. M., Nilsson, J. Å., Berglund, G., Molins, T., Esteban, J. I., Genescà, J., Paris, I., Haufroid, V., Selvais, Ph., Petit, J. M., Duong, M., Grappin, M., Guiguet, M., Rudoni, S., Portier, H., Brun, J. M., Bagg, W., Plank, L., Drury, P. L., Sharpe, N., Braatvedt, G. D., Carrascosa, J. M., Molero, J. C., Fermίn, Y., Andrés, A., Satrústegui, J., Rietzsch, H., Patzak, A., Schwanebeck, U., Simpson, H., Robertson-Mackay, F., Montegriffo, E., Fox, C., Chiasson, J.-L., Josse, R. G., Dorman, J. M., Gerstein, H. C., Lau, D., Leiter, L. A., Maheux, P., Meneilly, G. S., Murphy, L., Rodger, N. W., Ross, S. A., Ryan, E., Yale, J.-F., Wolever, T. M. S., Haller, T., Elias, I., Segal, P., Standi, E., Rybka, J., Sencer, E., Satman, I., Schlcnzka, A., Vakkilainen, J., Tsaglis, H., Ioannidis, I., Giakoumaki, A., Amantou, A., Komitopoulos, N., Georgiou, S., Varsamis, E., Katsilambros, N., El Gayar, M., Shereba, N., Botros, R., Fikry, R., Jackson, D., Balme, M., Silva-Nunes, J., Alves, J., Bogalho, P., Gardete-Correia, L., Nunes-Corrêa, J., Kot’átková, A., Němcová, D., Vrbíková, J., Zamrazil, V., Meyer, L., Delbachian, I., Lehert, P., Cugnardey, N., Drouin, P., Guerci, B., Wagner, O. F., Jones, N. P., Vallance, S. E., Thompson, K. A., Miller, A. K., Inglis, A. M. L., Patterson, S., Jorkasky, D., Freed, M. I., Mathisen, A. L., Schneider, R., Rubin, C., Houser, V., Beebe, K. L., Kortboyer, J. M., Eckland, D. J. A., Cranmer, H., Mori, Y., Kurokawa, N., Komiya, H., Horikoshi, H., Yokoyama, J., Tajima, N., Ikeda, Y., Bakst, A., Hemyari, P., Lönnqvist, F., Owen, S., Vikramadithyan, R. K., Chakrabarti, R., Misra, P., Prem Kumar, M., Sunil Kumar, K. B., Ghosh, A., Rajagopalan, R., Goldstein, B., Katoh, S., Tsuruoka, N., Hata, S., Matsushima, M., Ikemoto, S., Inoue, Y., Edwards, G., Fonseca, V., Biswas, N., Bakris, G., Viberti, G., Rebuck, A. S., Weill, S., Abel, M. G., Klappoth, W., Brodesser, A., Linkeschowa, R., Pushparaj, P., Tan, C. H., Tan, B. K. H., Bahner, A., Parker, J., Waite, G., Lipson, V., Nahar, N., Rokeya, B., Parveen, S., Nur-e-Alam, M., Mosihuzzaman, M., Hansen, A. Kornerup, Lepore°, M., Kurzhals, R., Pampanelli°, S., Fanelli°, C. G., Bolli°, G. B., Ratner, R. E., Hirsch, I. B., Mecca, T. E., Wilson, C. A., Mohideen, P., Mudaliar, S., Deutsch, R., Ciaraldi, T., Armstrong, D., Kim, B., Morrill, B., Sha, X., Henry, R., Meyer, B. H., Scholtz, H. 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- Published
- 1999
- Full Text
- View/download PDF
30. Specific Induction of a Functional Endogenous D2 Short Dopamine Receptor in GH4C1 Cells
- Author
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Allard, S., Lapointe, S., and Falardeau, P.
- Abstract
In contrast to lactotrophs, tumoral pituitary cells like GH3 and GH4C1 lack expression of dopamine D2short and D2long receptors. In GH4C1 cells, we observed that the expression of only the short isoform of D2 receptor can be induced after transfection with a plasmid which confers resistance to neomycin (pRSVNeo). High levels of fully functional D2short receptor were obtained in GH4C1 following transfection (528fmol/mg protein). Sequence, pharmacology and coupling of the induced-D2 receptor do not show any difference with the cloned rat D2 short receptor.
- Published
- 1993
- Full Text
- View/download PDF
31. In vivoinaccessibility of somatostatin receptors to 111Inpentreotide in primary renal cell carcinoma
- Author
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MONTRAVERS, F., ROUSSEAU, C., DOUBLET, J. D., GATTENGO, B., ALLARD, S., FOURET, P., BERNAUDIN, J. F., THIBAULT, P., and TALBOT, J. N.
- Abstract
The presence of somatostatin receptors on human renal cell carcinomas in surgically removed kidneys has been demonstrated by autoradiography. The aim of this study was to detect the in vivopresence of somatostatin receptors in primary renal tumours and their possible metastases before surgery, using 111In-pentreotide scintigraphy. 201T1 was used as a sensitive tumour-seeking agent with blood flow-dependent uptake. Fifteen patients were imaged before surgical removal of the renal tumour. Thirteen tumours were malignant. The large tumours (more than 4 cm in diameter) did not accumulate 111In-pentreotide or 201T1. In contrast, the single small tumour accumulated both tracers. A scalp skin metastasis was demonstrated in one patient by 201T1 and 111In-pentreotide uptake. In one case, known lung metastases were visualized with both 201T1 and 111In-pentreotide, but the lung metastases of another three patients as well as one case of epidural metastasis were not identified. In one patient with a photopaenic lesion, positive labelling of the surgically removed tumour was demonstrated by in vitroautoradiography. Somatostatin receptor scintigraphy with 111In-pentreotide appears to have little value for the detection of metastases in patients with renal cell carcinoma, as some metastases (especially those of the lungs) were missed. The absence of 111In-pentreotide uptake by large primary tumours is an interesting finding, suggesting inaccessibility of these very large tumours to drugs.
- Published
- 1998
32. Limitations of safranin ‘O’ staining in proteoglycan-depleted cartilage demonstrated with monoclonal antibodies
- Author
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Camplejohn, K. L. and Allard, S. A.
- Abstract
The intensity of safranin ‘O’ staining is directly proportional to the proteoglycan content in normal cartilage. Safranin ‘O’ has thus been used to demonstrate any changes that occur in articular disease. In this study, staining patterns obtained using monoclonal antibodies against the major components of cartilage proteoglycan chondroitin sulphate (anti CS) and keratan sulphate (anti KS), have been compared with those obtained with safranin ‘O’ staining, in both normal and arthritic tissues. In cartilage where safranin ‘O’ staining was not detectable, the monoclonal antibodies revealed the presence of both keratan and chondroitin sulphate. Thus, safranin ‘O’ is not a sensitive indicator of proteoglycan content in diseases where glycosaminoglaycan loss from cartilage has been severe.
- Published
- 1988
- Full Text
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33. Co-existent Anti-La Antibodies and Rheumatoid Factors Bear Distinct Idiotypic Markers
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Horsfll, A. C., Enables, P. J. W., Allard, S. A., and Maini, R. N.
- Abstract
This study describes the distribution of isotypes and idiotypes of two autoantibody populations, anti-La and rheumatoid factor, which co-exist in both the sera and saliva of patients with primary Sjögren's syndrome. The two autoantibodies are distinguished not only by their antigenic specificity but also by the nature of their idiotypic markers. IgA anti-La antibodies bearing restricted idiotypes are specifically enriched in saliva compared to serum suggesting their local synthesis. In contrast, rheumatoid factors bear cross-reactive idiotypes and may arise as a direct consequence of the secondary immune response.
- Published
- 1988
- Full Text
- View/download PDF
34. Comparative study of pharmacokinetics and serum bactericidal activities of cefpirome, ceftazidime, ceftriaxone, imipenem, and ciprofloxacin
- Author
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Paradis, D, Vallée, F, Allard, S, Bisson, C, Daviau, N, Drapeau, C, Auger, F, and LeBel, M
- Abstract
We compared the pharmacokinetics and the serum bactericidal activities of cefpirome, ceftazidime, ceftriaxone, imipenem, and ciprofloxacin. Fifteen healthy volunteers received 1 g of cefpirome, ceftazidime, and ceftriaxone intravenously, 500 mg of imipenem-cilastatin intravenously, and 500 mg of ciprofloxacin orally. High-performance liquid chromatographic assays were used to quantitate unchanged antibiotic in plasma and urine. Serum bactericidal activities were determined against six clinical isolates each of Staphylococcus aureus, Enterobacter cloacae, and Pseudomonas aeruginosa by using a modified microdilution method of Reller and Stratton (L. B. Reller and C. W. Stratton, J. Infect. Dis. 136:196-204, 1977). Overall, cefpirome exhibited pharmacokinetics similar to those of ceftazidime: half-life (t1/2), 1.95 h; concentration at 1 h (C1h), 47 to 49 micrograms/ml for both antibiotics. Ceftriaxone displayed the longest t1/2 (7.65 h) and the highest C1h (137.8 micrograms/ml), while we observed the shortest t1/2 (1.05 h) and the lowest C1h (19.85 micrograms/ml) with imipenem. At 1 h, cefpirome and, even more so, imipenem showed significantly better serum bactericidal activities against S. aureus (1:273 and 1:80) than did the other antibiotics (P less than 0.0005; analysis of variance with randomized block design and Bonferroni correction). Against E. cloacae, we observed the highest serum bactericidal titers at 1 h with cefpirome, and this superiority vis-à-vis the other antibiotics tested was maintained for up to 8 h after dosing. Ceftazidime remained the most active agent tested against P. aeruginosa (serum bactericidal activity titers, 1:43 at 1 h) up to 8 h. In summary, the study showed that cefpirome and imipenem provide more potent serum bactericidal activities than do broad-spectrum cephalosporins against S. aureus; thus, both of these antibiotics should be adequate against serious S. aureus infections. In addition, cefpirome appears to be a promising alternative for treatment of infections caused by E. cloacae and P. aeruginosa.
- Published
- 1992
- Full Text
- View/download PDF
35. Immuno-electron microscopy of chondrocyte-derived cells in the rheumatoid cartilage-pannus junction
- Author
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Muirden, K. D., Allard, S. A., Rogers, K., and Maini, R. N.
- Abstract
Immuno-electron microscopy has been utilised to examine the cartilage-pannus junction in seven patients with rheumatoid arthritis. Using a monoclonal antibody, keratan sulphate was localised to cells with the ultra-structural appearance of fibroblasts within a transitional fibroblastic zone in the pannus in two cases. This confirms previous light microscopy evidence that this area (which is clearly separate from articular cartilage) contains cells which produce keratan sulphate, and strengthens the hypothesis that these cells are derived from cartilage rather than from the adjacent synovial membrane.
- Published
- 1988
- Full Text
- View/download PDF
36. Chondrocyte-derived cells and matrix at the rheumatoid cartilage-pannus junction identified with monoclonal antibodies
- Author
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Allard, S. A., Muirden, K. D., Camplejohn, K. L., and Maini, R. N.
- Abstract
In the cartilage-pannus junction of 14 patients with rheumatoid arthritis (RA) and seven patients with osteoarthritis (OA), monoclonal antibodies to keratan sulphate (KS) and chondroitin sulphate (CS) stained a transitional fibroblastic zone (TFZ) within the pannus in nine RA patients and one OA patient. In three patients this was clearly localised to the cytoplasm of cells in this zone, but in all remaining cases KS and CS could be demonstrated in the surrounding matrix. This area was distinguished from adjacent pannus which contained many blood vessels and cells positive for MHC Class II antigen. Specific markers for glycosaminoglycans have been employed to demonstrate that chondrocyte-derived cells and matrix contribute to the changes seen at the cartilage-pannus junction in RA-affected joints.
- Published
- 1987
- Full Text
- View/download PDF
37. Cells and Matrix Expressing Cartilage Components in Fibroblastic Tissue in Rheumatoid Pannus
- Author
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Allard, S. A., Maini, R. N., and Muirden, K. D.
- Abstract
Our studies of the cartilage-pannus junction in rheumatoid joints have demonstrated the frequent presence of a transitional fibroblastic zone (TFZ) overlying articular cartilage. Using immunohistochemical techniques this zone has been shown to contain cells and matrix expressing specific cartilage components but not antigens present on cells in invasive vascular pannus. These findings support the concept that fibroblastic pannus is derived from the underlying articular cartilage rather than adjacent tissues. This type of reaction involving a metaplastic change in the chondrocyte is particularly common in large weight-bearing joints and may represent a different mechanism of cartilage degeneration to the classically described direct invasion of cartilage by hypertrophic synovial tissue.
- Published
- 1988
- Full Text
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38. Molecular characterization of two Brassica napus pollen-expressed genes encoding putative arabinogalactan proteins.
- Author
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Gerster, J, Allard, S, and Robert, L S
- Abstract
Two highly homologous cDNA clones, Sta 39-3 and Sta 39-4, corresponding to mRNAs highly expressed in Brassica napus cv Westar stamens were isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 39-3 and Sta 39-4 transcripts accumulate in pollen following the first pollen mitosis and are abundant at pollen maturity, thus identifying them as "late" pollen genes. Sta 39-3 and Sta 39-4 belong to a small gene family. Their predicted proteins share similarities with those deduced from recently isolated cDNAs encoding arabinogalactan proteins, which include a similar molecular mass (approximately 13 kD); high levels of alanine, proline, serine, and threonine, which are interspersed throughout the protein with no obvious repetitive motif; low levels of cysteine, histidine, tryptophan, and tyrosine; and hydrophobic N- and C-terminal ends. The Sta 39-3 and Sta 39-4 proteins may play a role in pollen germination and tube growth.
- Published
- 1996
- Full Text
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39. Suction-induced blister fluid penetration of cefdinir in healthy volunteers following ascending oral doses
- Author
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Richer, M, Allard, S, Manseau, L, Vallée, F, Pak, R, and LeBel, M
- Abstract
The pharmacokinetics and suction-induced blister fluid penetration of cefdinir following single oral administrations of 200, 300, 400, and 600 mg were studied in 16 healthy young male volunteers according to a Latin square design. Plasma, blister, and urine samples were assayed by high-pressure liquid chromatography. We observed a nonlinear relationship (P = 0.02) between the dose and the maximum concentration in plasma as well as between the dose and the area under the concentration-time curve (AUC) in plasma (P < 0.001), which may be indicative of a limited absorption process. This resulted in a lower AUC value than expected as well as a smaller fraction of cefdinir excreted unchanged at a dose of 600 mg. Renal clearance decreased with increasing doses (P < 0.006; analysis of variance with the Latin square design and Games-Howell procedure). Maximal cefdinir concentrations in blister fluid were delayed compared with concentrations in plasma. Blister fluid penetration measured by the ratio of the AUC in blister fluid to the AUC in plasma was extensive (92.4 to 108.4%). Cefdinir concentrations in blister fluid remained equal to or higher than the concentrations in plasma from 6 to 12 h following cefdinir administration. On the basis of the concentrations in blister fluid and the in vitro MIC data, we estimated that cefdinir at 200 to 400 mg administered twice daily would be adequate to treat uncomplicated skin infections caused by Streptococcus pyogenes. Seven volunteers experienced episodes of light-to-moderate diarrhea. These adverse events occurred irrespective of dose.
- Published
- 1995
- Full Text
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40. Réseaux moléculaires en toxicologie médico-légale : applications et perspectives
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Allard, S., Allard, P.-M., Morel, I., and Gicquel, T.
- Abstract
Nous appliquons à quatre cas médico-légaux une approche bio-informatique innovante pour l’organisation de données de spectrométrie de masse haute résolution : la génération de réseaux moléculaires. L’objectif est de montrer la pertinence de cette méthode dans le domaine de la toxicologie et d’illustrer son intérêt pour la comparaison et l’interprétation de données par la génération de réseaux multi-échantillons.
- Published
- 2018
- Full Text
- View/download PDF
41. Cimetidine-Induced Myelosuppression After Bone Marrow Transplantation
- Author
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Mehta, J., Powles, R. L., Treleaven, J., Shields, M., Agrawal, S., Rege, K., Mitchell, P., and Allard, S.
- Abstract
A strong temporal correlation was observed between cessation of cimetidine and a sustained increase in blood counts in two marrow transplant recipients. Both were receiving cimetidine from the day of transplantation for prophylaxis of stress ulceration and gastritis. The blood counts of both patients were not increasing satisfactorily 5-6 weeks after marrow transplantation without any obvious cause of marrow suppression. A similar observation has been made with the use of ranitidine after marrow transplantation suggesting that histamine H2-receptor antagonists should be used very cautiously, if at all, in the setting of bone marrow transplantation.
- Published
- 1994
- Full Text
- View/download PDF
42. 3-MeO-PCP et 4-MeO-PCP : confusion des isomères et risque majeur de toxicité
- Author
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Allard, S., Deslandes, G., Gaborit, B., Lomenech, H., Pineau, A., Jolliet, P., Garret, C., and Monteil-Ganiere, C.
- Abstract
Présenter un cas d’intoxication aiguë à un NPS lié à une erreur de produit consommé.
- Published
- 2017
- Full Text
- View/download PDF
43. Exploration of mephedrone metabolism by molecular networking in human urine samples
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Allard, S. and Verstraete, A.
- Abstract
There is a lack of tools for visualizing and organizing MS data of unknown compounds detected by untargeted toxicological screening. Molecular networking allows exploring and organising MS/MS data without prior knowledge of the sample's chemical composition. The organization of spectral data is based on spectral similarity. Hence, important information can be obtained even before the annotation step. The link established between molecules enables the propagation of structural information. We applied molecular networking to untargeted toxicological screening for the metabolites of mephedrone, a popular new psychoactive substance.
- Published
- 2019
- Full Text
- View/download PDF
44. Interférence de la venlafaxine et ses métabolites sur la recherche de buprénorphine par immunodosage CEDIA
- Author
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Allard, S., Grégoire, M., Monteil-Ganière, C., Dailly, E., Pineau, A., Azoulay-Fauconnier, C., Jolliet, P., and Deslandes, G.
- Abstract
Mise en évidence d’une interférence analytique impliquant la venlafaxine et ses métabolites lors de la recherche de buprénorphine urinaire par immunodosage CEDIA (Cloned Enzyme Donor Immunoassay).
- Published
- 2016
- Full Text
- View/download PDF
45. Synthacaïne : mosaïque de substances et d’effets. À partir d’un cas
- Author
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Deslandes, G., Monteil-Ganière, C., Grégoire, M., Allard, S., Marion, M., and Bouquié, R.
- Abstract
La « Synthacaïne » correspond à une nouvelle classe de substance psychoactive, vendue comme un stimulant légal et développée pour imiter les effets de la cocaïne. Elle est vendue sur des sites spécialisés sous le nom de « cocaïne légale » et disponible à des prix 2 à 4 fois inférieurs à ceux de la cocaïne. Nous rapportons ici le cas d’un homme de 30ans, consommateur régulier de divers produits stimulants (cocaïne, amphétamines et divers produits de synthèses commandés sur interne), hospitalisé pour un épisode d’angoisse aiguë associant un sentiment de mort imminente à des conduites d’automutilation. Il associe ses troubles à une consommation récente de synthacaïne acheté sur Internet « à des prix défiant toute concurrence ». À son arrivée, le patient dit ne pas avoir dormi pendant une semaine, et présente des hallucinations visuelles à type de dysmorphophobies, responsables d’angoisses massives conduisant à des actes d’automutilation (brûlures). Le patient décrit les effets de la synthacaïne comme étant différents de ceux de la cocaïne, qu’il a l’habitude de consommer, et avec laquelle ses expériences de paranoïa ne persistent pas. Aucun prélèvement biologique destiné à la recherche de toxique n’a été effectué. L’entourage du jeune homme nous a permis de récupérer le produit consommé acheté sur Internet.
- Published
- 2016
- Full Text
- View/download PDF
46. Réseaux moléculaires en toxicologie médico-légale: application à une suspicion d’intoxication à Tabernanthe iboga
- Author
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Allard, S., Allard, P., Le Devehat, F., Morel, I., and Gicquel, T.
- Abstract
Nous appliquons à un cas d’intoxication fatale due à l’ingestion d’une poudre vendue comme Tabernanthe iboga, une approche analytique utilisée dans le domaine de la chimie des substances naturelles: la génération de réseaux moléculaires. L’objectif est ici de déterminer l’identité botanique de la plante en cause dans le décès de la patiente et d’explorer les relations structurales entre les métabolites retrouvés dans les fluides biologiques et ceux présents dans la poudre végétale.
- Published
- 2018
- Full Text
- View/download PDF
47. Homicide-suicide par intoxication à l’acébutolol : une approche multimatrice par réseau moléculaire
- Author
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Gicquel, T., Allard, S., Le Daré, B., Baert, A., Bouvet, R., and Morel, I.
- Abstract
Nous présentons ici le cas d’une femme de 70 ans suspectée d’avoir agressé son mari à l’arme blanche, puis assassiné sa belle-fille handicapée avant de se suicider par ingestion d’acébutolol (Sectral®). En compléments des techniques analytiques classiques permettant l’identification et la quantification des xénobiotiques, nous avons utilisé une approche analytique originale : la génération de réseaux moléculaires.
- Published
- 2018
- Full Text
- View/download PDF
48. « Chemsex » : 2 cas de décès
- Author
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Allard, S., Monteil-Ganiere, C., Visseaux, G., Deslandes, G., and Clément, R.
- Abstract
Présenter deux cas de décès lors de « chemsex » : consommation de substances psychoactives dans un contexte sexuel.
- Published
- 2017
- Full Text
- View/download PDF
49. Spontaneous hydropneumothorax in a man with rheumatoid arthritis
- Author
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Tahir, H., Allard, S. A., and Jawed, S.
- Published
- 2001
50. Osteomyelitis of the humerus following steroid injections for tennis elbow
- Author
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Jawed, S. and Allard, S. A.
- Published
- 2000
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