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Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Horizonte Médico is the property of Universidad de San Martin de Porres and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This review aimed to determine the prevalence of the insecticide resistance status of the field-collected Aedes (Ae.) aegypti and Ae. albopictus in Malaysia from 2010 to 2022 towards carbamates, organochlorines, organophosphates and pyrethroids. Biological and environmental controls were summarized with an emphasis on the mosquito vector control strategies in Malaysia. The information in this review was extracted from several databases such as PubMed (MEDLINE), Science Direct and Scopus by using keywords including "insecticide resistance", "carbamate resistance", "organochlorine resistance", "organophosphate resistance", "pyrethroid resistance", "Aedes" and "Malaysia", between January 2022 and December 2022. Distribution of resistant Ae. aegypti and Ae. albopictus in Malaysia was mapped using QGIS software. Insecticide resistance in both Ae. aegypti and Ae. albopictus is widespread in Malaysia, although the rates vary by states. The most notable was the steep increase in permethrin resistance of Ae. aegypti in Selangor, Malaysia, over the past decade. Ae. albopictus also displayed moderate resistance to permethrin, though not as widespread as Ae. aegypti in Selangor, but showed sign of resistance in Sarawak, East Malaysia. Resistance towards four main classes of insecticides have been widely documented in Malaysia. The extensive resistance towards permethrin in Malaysia which is one of the current insecticides used in Malaysia suggested that policies supporting the widespread use of permethrin fogging needs further evaluation. [ABSTRACT FROM AUTHOR]

Objective: To determine the diversity of microbiota associated with different breeding habitats of dengue vector mosquitoes Aedes (Ae.) aegypti and Ae. albopictus and to identify any parasitic, epibiont, pathogenic, competitive or predatory species. Methods: Sampling was performed from a variety of breeding habitats using dipping, pipetting and siphoning techniques. Microbiota in water samples were preserved using Rose Bengal solution and Lugol's iodine, and were identified. Live samples of microbiota were kept under laboratory conditions to observe any pathogenic or parasitic microbiota interacting with larvae. Results: A total of eleven microbiota species (Canthocamptus staphylinus, Canthocamptus microstaphylinus, Parastenocaris brevipes, Lepadella ovalis, Lepadella patella, Rotatoria rotatoria, Rotatoria macrura, Asplanchna brightwelli, Trichocerca rattus, Euglena variabilis, and Flagilaria capucina) belonging to four (4) phyla (Arthropoda, Rotifera, Euglenozoa, and Ochrophyta) and 8 microbiota species belonged to four phyla (Arthropoda, Rotifera, Euglenozoa, and Ochrophyta) were identified from Ae. aegypti and Ae. albopictus breeding habitats respectively. There was a higher percentage (54.54%) of larval habitats positive for the secondary vector Ae. albopictus than through the primary vector Ae. aegypti in the Gampola urban area indicating higher possibility of transmitting the dengue virus through the secondary vector. However, no pathogenic or parasitic ciliates on mosquito larvae were encountered in the present study. Those findings may be due to sampling maingly from temporary container-type breeding habitats. Conclusions: The relative distribution of microbiota associated with mosquito species differed significantly among Ae. aegypti and Ae. albopictus. The overall findings of this study could help in implementing novel eco-friendly vector-control strategies in the study area. [ABSTRACT FROM AUTHOR]

Introduction: This article analyzes risk discourses around dengue, zika and chikungunya constructed by lay people, community leaders and disease control experts from the fields of medical anthropology, medical sociology, and public health. Methods: A qualitative ethnographic study was conducted in a municipality in Colombia (December 2016 and January 2018) with semistructured and open-ended interviews, informal dialogues, and fieldwork journal observations. Results: This study found a mismatch in risk discourse about vector-borne diseases among health officials, lay people, and community leaders. These discourses are linked to the sociocultural contexts in which people live, and offer particular ways of giving meaning and acting in the face of disease prevention. Conclusion: The findings show a multisituated risk that refers to the inside and outside of homes; and the prevention practices mentioned by different actors, in which a continuity of tensions between lay people, leaders and government officials can be observed. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Saúde Coletiva is the property of MPM Comunicacao and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Parasite (1252607X) is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Zika virus (ZIKV) was identified in 1947 in a rhesus monkey during an investigation of the yellow fever virus in the Zika Forest of Uganda; it was also isolated later from humans in Nigeria. The main distribution areas of ZIKV were the African mainland and South-East Asia in the 1980s, Micronesia in 2007, and more recently the Americas in 2014. ZIKV belongs to the Flaviviridae family and Flavivirus genus. ZIKV infection, which is transmitted by Aedes mosquitoes, is an emerging arbovirus disease. The clinical symptoms of ZIKV infection are fever, headache, rashes, arthralgia, and conjunctivitis, which clinically resemble dengue fever syndrome. Sometimes, ZIKV infection has been associated with Guillain-Barré syndrome and microcephaly. At the end of 2015, following an increase in cases of ZIKV infection associated with Guillain-Barré syndrome and microcephaly in newborns in Brazil, the World Health Organization declared a global emergency. Therefore, considering the global distribution and pathogenic nature of this virus, the current study aimed at reviewing the virologic features, transmission patterns, clinical manifestations, diagnosis, treatment, and prevention of ZIKV infection. [ABSTRACT FROM AUTHOR]

In the rapidly urbanizing region of West Africa, Aedes mosquitoes pose an emerging threat of infectious disease that is compounded by limited vector surveillance. Citizen science has been proposed as a way to fill surveillance gaps by training local residents to collect and share information on disease vectors. Understanding the distribution of arbovirus vectors in West Africa can inform researchers and public health officials on where to conduct disease surveillance and focus public health interventions. We utilized citizen science data collected through NASA’s GLOBE Observer mobile phone application and data from a previously published literature review on Aedes mosquito distribution to examine the contribution of citizen science to understanding the distribution of Ae. aegypti in West Africa using Maximum Entropy modeling. Combining citizen science and literature-derived observations improved the fit of the model compared to models created by each data source alone but did not alleviate location bias within the models, likely due to lack of widespread observations. Understanding Ae. aegypti distribution will require greater investment in Aedes mosquito surveillance in the region, and citizen science should be utilized as a tool in this mission to increase the reach of surveillance. [ABSTRACT FROM AUTHOR]

Mosquitoes were collected in Lake County, CA, in 2014 and tested using standard polymerase chain reaction for filarial parasite DNA. Filarial parasites were detected in 23 out of 1,008 total pools. DNA from Dirofilaria immitis, the parasite causing dog heartworm, was detected in Aedes increpitus (MIR=4.62), Aedes sierrensis (MIR=6.72), Anopheles freeborni (MIR=1.08), and Culex tarsalis (MIR=0.10). Setaria yehi, deer body worm, was detected in Ae. sierrensis (MIR=13.42), Anopheles franciscanus (MIR=0.55), An. freeborni (MIR=2.69), and Culex stigmatosoma (MIR=0.41). The avian parasite Splendidofilaria could not be identified to species but was detected in Cx. tarsalis (MIR=0.20). DNA was also detected for three unidentified filarial parasites in Culex. Filarial-positive pools spanned May-August, with Splendidofilaria earlier in the season and S. yehi later. For D. immitis, MIR tended to be highest in June, when the 130 HDU development threshold was reached. Interestingly, D. immitis was also detected prior to the HDU threshold, and D. immitis was not detected August-September, though HDU remained high enough for development. This suggests that there are other factors influencing dog heartworm transmission in the area. [ABSTRACT FROM AUTHOR]

The aim of this study was to provide a synthesis of published studies investigating education-based arbovirus control strategies. The data were collected from the LILACS, BDENF and MEDLINE databases using the descriptors "Health Education" and "Aedes", together with the Boolean operator "AND". The searches retrieved 242 studies, 14 of which were included in the review after applying the inclusion and exclusion criteria. The data were analyzed using a qualitative approach, resulting in the identification of four categories: vector control actions based on community capacity building; social mobilization for arbovirus control; education-based vector control combined with biological control; and integrated arbovirus control actions. The findings show that health education is an essential element of arbovirus control and should be implemented in conjunction with other vector control strategies. [ABSTRACT FROM AUTHOR]

Copyright of Cogitare Enfermagem is the property of Cogitare Enfermagem and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Display omitted] • Liposcelis sp can predate Aedes aegypti eggs loose in environment. • Aedes aegypti eggs are more suscetible to predation by Liposcelis sp when fixed in substrate. • Aedes aegypti larvae which survive exposure to Liposcelis sp has slowed immature development. For different research purposes, there is a need to mass rear mosquitoes, such as Aedes aegypti, under laboratory conditions. The rearing process begins with egg production followed by egg storage in a dry environment, inside containers. Stored eggs are susceptible to environmental threats when storage conditions are suboptimal. Some terrestrial insects can invade this environment and attack stored eggs. In this brief report, we assessed whether Ae. aegypti eggs exposed to Liposcelis sp. individuals had reduced hatching and immature development rates. We exposed 100 eggs in different treatment conditions (fixed in porous paper and loosed) to 30 Liposcelis sp. individuals for ten days and then we induced hatching. We observed a hatching rate of 99% reduced for those eggs adhered to porous paper and loosed eggs showed a hatching rate of 45% decreased for those eggs exposed to Liposcelis sp. The remaining larvae took longer to develop into pupae as well, showing a four-day delay on average to the final metamorphosis of the aquatic stage. These results reinforce the need to frequently monitor egg storage conditions to maintain laboratory colonies stable and free from pests that can interfere with mosquito life-history traits. [ABSTRACT FROM AUTHOR]

Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Global rises in dengue hemorrhagic fever, especially in Asia and Latin America, underscore the necessity for enhanced public health interventions. Aedes spp. mosquitoes are the primary vectors; however, species such as Culex quinquefasciatus pose significant health risks by transmitting diseases such as filariasis, impacting millions of people worldwide. This study introduces a real-time convolutional neural network-based mosquito classification system using wingbeat frequency for identifying various mosquito species, with emphasis on Aedes sp. We proposed and assessed two models: a binary classification and a multiclass system. The binary system exhibited an outstanding accuracy of 91.76% in distinguishing between Aedes aegypti and Culex quinquefasciatus. The multiclass system accurately identified female and male Aedes aegypti and Culex quinquefasciatus with a precision of 87.16%. This innovative approach serves as a potential tool for dengue infection control and a versatile instrument for combating various mosquito-borne illnesses, enhancing vector surveillance for comprehensive disease management. • Developed a CNN-based mosquito classification system to identify mosquito species. • Assessed two models: binary classification and multiclass systems. • This tool is useful for controlling dengue infection and mosquito-borne illnesses. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Ciência & Saúde Coletiva is the property of Associacao Brasileira de Pos-Graduacao em Saude Coletiva and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To evaluate locomotor activity in four field populations of Ae. aegypti with different insecticide resistance profiles from the state of São Paulo for two years. METHODS: This study comprised the susceptible Rockefeller strain and four populations from São Paulo, Brazil: two considered populations with “reduced susceptibility” to pyrethroids (Campinas and Marília), and two “resistant populations” (Santos and Ribeirão Preto). First, 2016 and 2017 eggs from these five populations were hatched in laboratory. Virgin females underwent experiments under laboratory conditions at 25°C, with 12:12h light/dark (LD) photoperiod; 24-hour individual activity was recorded using a locomotor activity monitor (LAM). RESULTS: In females from 2016 field populations, both resistant populations showed significant more locomotor activity than the two reduced susceptibility populations and the Rockefeller strain (p < 0.05). As for females from 2017 field populations, reduced susceptibility populations showed a significant increased locomotor activity than the Rockefeller strain, but no significant difference when compared to Santos resistant population (p > 0.05). CONCLUSIONS: Our results indicate that insecticide-resistant Ae. aegypti populations show increased locomotor activity, which may affect the transmission dynamics of their arboviruses. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Jumantik officers are government officials who have the duty to check the presence of aedes aegpyti mosquito larvae in the community. The purpose of this research is to help Jumantik officers and village officials to carry out the process of checking mosquito larvae. This application is can be used to assist officers in creating reports for the inspection of mosquito larvae, helping officers to report the problematic addresses and scheduling for inspection of mosquito larvae. This application was developed using the React Native framework and Javascript programming language. The results of this study are useful applications as a substitute for reporting the presence of conventional mosquito larvae. [ABSTRACT FROM AUTHOR]

Les moustiques sont les principaux responsables de la transmission d'arbovirus (Arthropod-borne virus) qui ont pour caractéristique d'être des virus transmis par des arthropodes vecteurs. Parmi les moustiques vecteurs d'arboviroses, les Aedes et les Culex occupent une place prépondérante. Aedes (Stegomyia) aegypti , présent dans les régions tropicales et subtropicales, est le principal vecteur pour les virus de la dengue, du chikungunya et du virus Zika. Il est responsable d'épidémies de grande ampleur. Aedes (Stegomyia) albopictus est une espèce originaire d'Asie du Sud-Est qui s'est implanté dans de nombreuses zones tempérées et dont l'implantation progresse en France métropolitaine. Ce virus est responsable des cas autochtones de dengue, chikungunya et Zika régulièrement observés en France depuis une dizaine d'années. Culex modestus et Culex pipiens sont des vecteurs pour le virus West-Nile qui est en recrudescence dans le sud de la France. Les moustiques présents en France métropolitaine peuvent être également vecteurs pour les virus Usutu et Tahyna. L'implantation de nouvelles espèces de moustiques vecteurs ou l'importation de virus pouvant être transmis par des moustiques présents en France métropolitaine sont des hypothèses à considérer. Mosquitoes are mainly responsible for the transmission of arboviruses (Arthropod-borne viruses) characterized by their transmission by arthropod vectors. Among the mosquitoes transmitting arboviruses, Aedes and Culex genera play a major role. Aedes (Stegomyia) aegypti , found in tropical and subtropical regions, is the main vector for dengue, chikungunya and Zika viruses and is responsible for large-scale epidemics. Aedes (Stegomyia) albopictus is a species native to Southeast Asia that has spread to many temperate countries including metropolitan France. This virus is responsible for the autochthonous cases of dengue, chikungunya and Zika regularly observed in France for about ten years. Culex modestus and Culex pipiens are vectors for the West-Nile virus whose incidence is increasing in the south of France. Mosquitoes present in Metropolitan France may also be vectors for Usutu and Tahyna viruses. The implantation of new species of mosquito vectors or the importation of viruses that can be transmitted by mosquitoes present in metropolitan France are hypotheses to be considered. [ABSTRACT FROM AUTHOR]

En entomologie médicale et vétérinaire, les moustiques ont une place importante à la fois comme sources de nuisances en lien avec leur hématophagie, mais aussi comme vecteurs potentiels de pathogènes. Sont abordés ici la diversité des moustiques dont près de 3 500 espèces différentes sont connues, mais aussi des pathogènes transmis. Environ 300 espèces différentes de moustiques peuvent piquer les humains à travers le monde. Seules une centaine de cellesci sont reconnues comme vectrices potentiels d'agents pathogènes dangereux. Les espèces les plus dangereuses peuvent être regroupées dans les trois genres Aedes, Anopheles et Culex. Arbovirus, protozoaires (Plasmodium sp.) et helminthes (filaires) sont à l'origine de pathologies que l'on rencontre aussi bien en régions tropicales qu'en régions tempérées. Les principales méthodes de lutte sont évoquées. In medical and veterinary entomology, mosquitoes have an important place both as sources of nuisance in connection with their hematophagy, but also as potential vectors of pathogens. This article reviews the diversity of mosquitoes of which nearly 3 500 different species are known, but also of the pathogens transmitted. About 300 different species of mosquitoes can bite humans around the world. Only a hundred of these are recognized as potential vectors of dangerous pathogens. The most dangerous species can be grouped into the three genera Aedes, Anopheles and Culex. Arboviruses, protozoa (Plasmodium sp.) and helminths (filariae) are the cause of pathologies that are encountered in both tropical and temperate regions. The main control methods are discussed. [ABSTRACT FROM AUTHOR]

Interspecific associations between two mosquito species can lead to effects such as competition, species displacement, and species stability. To better understand Aedes albopictus (Skuse) and other Aedes species, we monitored eggs in artificial oviposition cups (ovitraps) within Knox County, TN, U.S.A., during the 2016 and 2017 mosquito seasons. In 2016, one black and one white ovitrap were placed at 18 sites for 21 weeks, while in 2017 black and white ovitraps baited with grassinfused or deionized water were placed at 11 sites for nine weeks. Eggs were identified to species and resulting counts were used to determine the degree of interspecific association using Cole's coefficients (C7) and the degree of heterogeneity across space and time using generalized linear mixed models (GLMM). Here, Aedes mosquitoes are generally ovipositing in black cups with grass-infused water, and Ae. albopictus eggs co-occurred with other Aedes species more often than would be expected. Finding a positive significant interspecific association between Ae. albopictus and other Aedes eggs suggests that methods used to control Ae. albopictus may also control other Aedes mosquitoes. Finding that Ae. albopictus co-occurs with other Aedes mosquitoes warrants additional research to evaluate outcomes associated with co-occurrence within the study area. [ABSTRACT FROM AUTHOR]

Essential oils from aerial parts of the herbs Peganum harmala and Nepeta cataria , and leaves of the tree Phellodendron amurense were analyzed by GC-FID and GC-MS, and their larvicidal activities were assayed on the early fourth instar larvae of Aedes aegypti. The major constituents of the oils were limonene (14.5%) and thymol (11.5%) in P. harmala , thymol (46.5%), 4aα,7α,7aβ-nepetalactone (18.3%) and 4aα,7β,7aα-neptalactone (19.7%) in N. cataria , eugenol (14.5%) andγ-eudesmol (9.5%) in P. amurense.The oil of N. cataria had a strong larvicidal activity (LC 50 < 50 µg/mL; LC 90 < 86.8 µg/mL) on A. aegypti while the remaining oils showed a moderated killing effect. The larvicidal activity of N. cataria oil was associated to the contents of 1,8-cineol, camphor, 4aα,7α,7aβ-Nepetalactone, 4aα,7β,7aα-Nepetalactone and thymol. Our results indicate that the oil of N. cataria deserves to be used as a source of larvicidal agents against A. aegypti. [ABSTRACT FROM AUTHOR]

Copyright of InfoDesign: Revista Brasileira de Design da Informação is the property of Infodesign: Revista Brasileira de Design da Informacao and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Dengue fever is the most common mosquito-borne viral disease in the world, caused by the dengue viruses (DENV 1–4) and it was transmitted in Malaysia by two main Aedes mosquito species: Aedes aegypti and Aedes albopictus. The overuse of synthetic chemical insecticides in managing these vectors leads to an increase in insecticide resistance, which has occurred in most arthropod species, including Aedes mosquitoes. Bio-insecticides have been suggested as a new potential alternative method which can replace synthetic chemical insecticides to overcome the vector issues. Analytical data were used to compare with Malaysian plants that have larvicidal ability; and those plants were Areca catechu, Azolla pinnata, Lantana camara, Mukia maderaspatana, and Leucas aspera. The most prevalent chemical components found in all five plants were fatty acids (oleic acid, palmitic acid, tetradecanoic acid), fatty acid methyl esters (hexadecanoic acid methyl ester, 9-octadecenoic acid (Z)-methyl ester), and flavonoids (catechin). These chemical compounds have been patented for pesticide formulations due to their biodegradable qualities and capacity to increase pesticide efficiency. Furthermore, the key advantages of these chemical compounds to combat vector difficulties are their enzyme inhibitory characteristics, biochemical alterations, and structural deformation of mosquito larvae. According to the findings of this study, these five plants have the potential to be used as bio-insecticides. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aedes aegypti is currently a critical disease agent and is responsible for viruses such as Zika, Chikungunya and Dengue's four serotypes. This mosquito's relevance to the current social body has come to the fore and triggered urgent EcoHealth investigations since this approach aims to articulate different theoretical fields to understand the historical linkages between nature, society and health. Based on an ethnographic premise, this study considered the unequal and unfair conditions that make women's health vulnerable to dengue, analyzing their practices and perceptions about the potential breeding grounds in the public space. A semi-structured interview and participant observation, as well as a field diary, were used to compose the study. The research included the participation of ten women living in the outskirts of Fortaleza, Ceará, Brazil, from January to August 2014. The category "Social inequality, context and practices in the public space" emerged from the content analysis. The narratives revealed that unstable living conditions and evident social inequality might influence in a context permeated by waste, with great potential for dengue's mosquito proliferation. [ABSTRACT FROM AUTHOR]

Mosquito larvae were collected from the subalpine region of the eastern Sierra Nevada Mountains from 2011 to 2014. Two watersheds were sampled and sites selected were mainly vernal snow-melt pools and wet meadows. Seven Aedes species, Culiseta incidens (Thomson), and Culex tarsalis Coquillett were collected. The most abundant and widely distributed species were Ae. hexodontus Dyar and Ae. tahoensis Dyar. Aedes tahoensis was the predominate species in woodland snowmelt habitats. Some species were found at most elevations while others were found more often at specific elevations. The most restrictive species was Ae. ventrovittis Dyar which occurred almost exclusively between 3,219 m a.s.l. and 3,390 m a.s.l. Shannon and Simpson species diversity indices demonstrated that species diversity was greater in meadow habitats compared to woodland habitats. Mixed woodland/meadows, rock pools, and shallow grass pools were intermediate in species diversity. Abiotic factors such as snowpack and water temperature impacted species development times and when habitats dried. It was concluded that spatial and temporal patterns of habitats, along with elevation, influenced species presence and larval development. The results of the present study and previous work in the eastern Sierras will help guide future research that focuses on the potential change in the distribution and seasonality of subalpine mosquitoes and disease potential in the eastern Sierras as climatic conditions change. [ABSTRACT FROM AUTHOR]

In the present study, the effects of two spatial repellents (SR) were determined for Aedes aegypti and Ae albopictus, the main vectors of dengue, Chikungunya, and Zika fever. The modular high-throughput screening system (HITSS) was used to evaluate the response of both species to transfluthrin and linalool SR at different concentrations. The highest spatial repellency results for Ae. aegypti were obtained by transfluthrin to 0.001% with 37.50 ± 4.33%, and for linalool to 10% with 77.50 ± 3.90%. For Ae. albopictus, the highest spatial repellency percentages for transfluthrin 0.01% were 45.00 ± 3.78%, and linalool at 1% and 10% were 56.25 ± 7.06% and 56.25 ± 6.46%, respectively. Transfluthrin caused high levels of mortality with 71.25 ± 6.66%, 79.75 ± 8.65%, and 100% to Ae. aegypti and 70.00 ± 5.98% and 98.75 ± 0.82% to Aedes albopcitus. With the results of this study, we concluded that both the transfluthrin and linalool could be used as protection measures against the bite of Ae. aegypti and Ae. albopictus in the integral strategies for the control of vectors in Mexico. [ABSTRACT FROM AUTHOR]

Mosquito vectors play a crucial role in the distribution of avian Plasmodium parasites worldwide. At northern latitudes, where climate warming is most pronounced, there are questions about possible changes in the abundance and distribution of Plasmodium parasites, their vectors, and their impacts to avian hosts. To better understand the transmission of Plasmodium among local birds and to gather baseline data on potential vectors, we sampled a total of 3,909 mosquitoes from three locations in south-central Alaska during the summer of 2016. We screened mosquitoes for the presence of Plasmodium parasites using molecular techniques and estimated Plasmodium infection rates per 1,000 mosquitoes using maximum likelihood methods. We found low estimated infection rates across all mosquitoes (1.28 per 1,000), with significantly higher rates in Culiseta mosquitoes (7.91 per 1,000) than in Aedes mosquitoes (0.57 per 1,000). We detected Plasmodium in a single head/thorax sample of Culiseta, indicating potential for transmission of these parasites by mosquitoes of this genus. Plasmodium parasite DNA isolated from mosquitoes showed a 100% identity match to the BT7 Plasmodium lineage that has been detected in numerous avian species worldwide. Additionally, microscopic analysis of blood smears collected from black-capped chickadees (Poecile atricapillus) at the same locations revealed infection by parasites preliminarily identified as Plasmodium circumflexum. Results from our study provide the first information on Plasmodium infection rates in Alaskan mosquitoes and evidence that Culiseta species may play a role in the transmission and maintenance of Plasmodium parasites in this region. [ABSTRACT FROM AUTHOR]

Mosquitoes interact with the microbiome of the waters where they oviposit in several ways. Past work suggests adult mosquitoes can detect certain microbes that stimulate oviposition. The presence or absence of certain microbes in water containers thus can attract or repel mosquito species to different containers. I hypothesized that these relationships could be detected via metagenomics. I focused on two container breeders that coexist in Southern Taiwan: the dengue vector Aedes aegypti and the less competent vector Ae. albopictus. In addition to culturing, I performed 16S and 18S rDNA metagenomics assays, the latter of which had never been applied to mosquito waters before, to identify the microbial diversity of artificial containers with and without mosquito larvae. I found no correlation between mosquito presence to any features of the containers or to their microbiomes, which instead correlated strongly with location. Microbial diversity across containers was highly variable, even within the same location, with multiple taxa only found in single containers. This variability is reasonable, because mosquito gut microbiomes are also extremely variable. The possibility remains that microbes in natural containers differ significantly from those in artificial containers, and that these differences drive Aedes preferences for human-associated containers. Broad, single-microbe experimental work is recommended to identify possible attractant or repellent microbial taxa. Unlabelled Image • Mosquitoes can detect the presence of microbes in water containers. • Bacterial and eukaryote diversity was described with metagenomics and culturing. • Container microbiota correlates with location, but not with Aedes presence. • Multiple microbe species in a community may attract mosquitoes equally. [ABSTRACT FROM AUTHOR]

Copyright of Cadernos de Saude Publica is the property of Escola Nacional de Saude Publica Sergio Arouca and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Zika virus (ZIKV) is spread among human populations primarily through the bite of Aedes mosquitoes. While most ZIKV infections are asymptomatic or cause self-limited symptoms, the major concerns are its association with Guillain-Barré Syndrome and fetal microcephaly together with other birth defects, known as congenital Zika syndrome (CZS). This article reviews the confirmed Zika cases in the continental United States (U.S.) and Hawai'i thus far, as well as literature of Zika research relevant to Hawai'i. The first case of CZS within the U.S. was reported in Hawai'i, highlighting the unique position of Hawai'i for emerging and re-emerging infectious diseases. Recent studies of the Zika outbreak in Florida demonstrate the key role of Ae. aegypti mosquito in transmission; continuous and proactive vector surveillance in Hawai'i is warranted. Additionally, an updated interim pregnancy guidance for pregnant women with possible ZIKV exposure was summarized. Due to recent decline of ZIKV transmission in the Americas, the risk of ZIKV importation to Hawai'i has been greatly reduced. However, given the presence of Aedes mosquitoes, climate condition, and status of Hawai'i as a travel destination and foreign import market, public health officials and healthcare providers should remain vigilant for a potential outbreak of mosquito-borne diseases in the future. [ABSTRACT FROM AUTHOR]

Consensus guidelines for treatment of dengue fever from the World Health Organization and US Centers for Disease Control recommend acetaminophen to manage pain and fever but contraindicate nonsteroidal anti-inflammatory agents (NSAIDs) because of potentially increased bleeding risk, with thrombocytopenia as a complication. Neither acetaminophen nor ibuprofen (the NSAID with lowest bleeding risk) have been evaluated for dengue treatment in randomized, controlled clinical trials. Epidemiologic and cohort studies and case series describing NSAID use in dengue generally point to minimal or no significant increase in bleeding risk, except for aspirin. Given the lack of data on use of NSAIDs in dengue, we assessed the literature for the risk of postoperative bleeding with NSAID use, with a particular focus on ibuprofen, as a potential surrogate marker of bleeding risk in dengue. Ibuprofen at over-the-counter doses used to treat pain and fever (i.e. up to 1,200 mg/d for up to 10 days) is associated with zero to minimally increased risk for postoperative bleeding events. Where detected, statistically significant increases in bleeding incidence and/or bleed volume were not clinically meaningful. Because hepatitis is also a frequent dengue complication, acetaminophen-associated hepatotoxicity (the most common cause of drug-induced liver disease and acute liver failure in the United States and Europe) raises the possibility of severe hepatic injury with acetaminophen treatment. Data suggesting that conditions associated with chronic liver damage reduce the dosing threshold for induction of liver failure are of particular concern. Meta-analyses of clinical studies across a range of clinical settings consistently conclude that ibuprofen, at non-prescription doses, provides equivalent or superior analgesic and antipyretic activity compared with acetaminophen, with comparable safety. These data suggest that the consensus guideline recommendations for acetaminophen and against NSAID use in dengue treatment should be reconsidered in light of current evidence regarding the risks and benefits of each agent. [ABSTRACT FROM AUTHOR]

Copyright of Eastern Mediterranean Health Journal is the property of World Health Organization and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Parasite (1252607X) is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de Atencao Primaria a Saude is the property of Revista de Atencao Primaria a Saude and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Karyotypes of Aedes (Culicidae) mosquitoes (Ae. excrucians, Ae. behningi, and Ae. euedes) have been analyzed using the metaphase chromosomes of imaginal discs. Lacto-aceto-orcein, C-banding, and DAPI staining have detected speciesspecific features in the morphology and lengths of these chromosomes in the examined species. Species-specific features of chromosome 1 in the location of heterochromatin blocks have been shown. Thus, the metaphase chromosomes in the imaginal discs of Ae. excrucians, Ae. behningi, and Ae. euedes are a characteristic for species identification of mosquito species. [ABSTRACT FROM AUTHOR]

Plastic in the form of microplastic particles (MPs) is now recognized as a major pollutant of unknown consequences in aquatic habitats. Mosquitoes, with aquatic eggs, larvae, and pupae, are likely to encounter microplastic, particularly those species that are abundant in close proximity to human development, including those that vector human and animal disease. We examined the effects of polyethylene MPs, the most common microplastic documented in environmental samples, on the development and survival of the mosquitoes Aedes albopictus and Culex quinquefasciatus. In laboratory egg-laying and larval development container environments similar to those used by both species in the field, a mix of 1–53 µm MPs at concentrations of 60, 600, and 6000 MP ml−1 increased early instar larval mortality in both species relative to control treatments. A significant difference was found in the response of each species to microplastic at the lowest microplastic concentration tested, with Cx. quinquefasciatus survival equivalent to that in control conditions but with Ae. albopictus larvae mortality elevated to 37% within 48 h. These results differ from those of previous studies in which larvae were only exposed to MPs during the last aquatic instar stage and from which it was concluded that microplastic was ontogenically transferred without negatively affecting development. Increasing plastic pollutant concentrations could therefore act as selective pressures on aquatic larvae and ultimately influence outcomes of ecological interactions among mosquito vector populations. • Microplastic particles are increasingly common pollutants in most ecosystems. • Polyethylene particles 1–53 µm increase early mosquito larvae mortality. • Disease-vectoring mosquito species have differing responses to microplastics. • Aedes larvae incur high mortality at a plastic level tolerated by Culex larvae. • Ingested polyethylene is not typically transferred across mosquito life stages. [ABSTRACT FROM AUTHOR]

Dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. Pteridophytes were identified to be a significant source of novel mosquitocidal agents. The present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., Actiniopteris radiata , Adiantum caudatum , Cheilanthes swartzii , Hemionitis arifolia and Lycopodium clavatum . The larvicidal potential of the extracts screened using larvae of dengue vector Aedes aegypti (III and IV instar) and malarial vector Anopheles stephensi (III and IV instar), showed 10–100% mortality rates. Biosafety assessment was made on embryos of Danio rerio and Artemia nauplii . The phyto-constituents of the methanol extract of A. radiata leaves were identified through gas chromatography-mass spectrometry (GC-MS). Methanolic leaf extracts of A. radiata , A. caudatum and C. swartzii exhibited larvicidal activity against III and IV instar larvae of Ae. aegypti (LC 50 : 37.47, 74.51 and 152.38 and 67.58, 95.89 and 271.46 ppm) and An. stephensi (LC 50 : 70.35, 112.12 and 301.05 and 113.83, 175.30 and 315.19 ppm), respectively. The GC-MS of the methanol extract of A. radiata leaves revealed the presence of 7 phyto-components among which, Carbamic acid, phenyl-, (2-Nitrophenyl) methyl ester ( 1 ), Benzoic acid, 3- methylbenzoate ( 2 ) and 4-(benzylimino)− 1,4-dihydro-1-(p-toluoylmethyl) pyridine ( 3 ) were dominant. Biosafety assessment of methanol extract of A. radiata leaves on embryos of Danio rerio (Zebra fish) and Artemia nauplii (micro crustacean) revealed that there were no destructive or teratogenic effects. To conclude, the larvicidal activity and insignificant toxicity to non-target aquatic organisms of A. radiata leaves makes it a potential and environment safe biocontrol agent against dengue and malarial vectors. [ABSTRACT FROM AUTHOR]

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)