8 results on '"Jia, Yangyang"'
Search Results
2. Global marine microbial diversity and its potential in bioprospecting
- Author
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Chen, Jianwei, Jia, Yangyang, Sun, Ying, Liu, Kun, Zhou, Changhao, Liu, Chuan, Li, Denghui, Liu, Guilin, Zhang, Chengsong, Yang, Tao, Huang, Lei, Zhuang, Yunyun, Wang, Dazhi, Xu, Dayou, Zhong, Qiaoling, Guo, Yang, Li, Anduo, Seim, Inge, Jiang, Ling, Wang, Lushan, Lee, Simon Ming Yuen, Liu, Yujing, Wang, Dantong, Zhang, Guoqiang, Liu, Shanshan, Wei, Xiaofeng, Yue, Zhen, Zheng, Shanmin, Shen, Xuechun, Wang, Sen, Qi, Chen, Chen, Jing, Ye, Chen, Zhao, Fang, Wang, Jun, Fan, Jie, Li, Baitao, Sun, Jiahui, Jia, Xiaodong, Xia, Zhangyong, Zhang, He, Liu, Junnian, Zheng, Yue, Liu, Xin, Wang, Jian, Yang, Huanming, Kristiansen, Karsten, Xu, Xun, Mock, Thomas, Li, Shengying, Zhang, Wenwei, and Fan, Guangyi
- Abstract
The past two decades has witnessed a remarkable increase in the number of microbial genomes retrieved from marine systems1,2. However, it has remained challenging to translate this marine genomic diversity into biotechnological and biomedical applications3,4. Here we recovered 43,191 bacterial and archaeal genomes from publicly available marine metagenomes, encompassing a wide range of diversity with 138 distinct phyla, redefining the upper limit of marine bacterial genome size and revealing complex trade-offs between the occurrence of CRISPR–Cas systems and antibiotic resistance genes. In silico bioprospecting of these marine genomes led to the discovery of a novel CRISPR–Cas9 system, ten antimicrobial peptides, and three enzymes that degrade polyethylene terephthalate. In vitro experiments confirmed their effectiveness and efficacy. This work provides evidence that global-scale sequencing initiatives advance our understanding of how microbial diversity has evolved in the oceans and is maintained, and demonstrates how such initiatives can be sustainably exploited to advance biotechnology and biomedicine.
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- 2024
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3. Re-Evaluating PIN1 as a Therapeutic Target in Oncology Using Neutral Inhibitors and PROTACs
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Liu, Chuan, Chen, Zhonghui, Chen, Tao, Song, Hongmei, Shen, Jianbo, Yuan, Xiaoxi, Xia, Shuai, Liu, Qian, Chen, Qiuxia, Tian, Qiang, Meng, Xiaoyun, Han, Zhu, Dong, Xiaofei, Yang, Yu, Cai, Longying, Cheng, Xuemin, Jia, Yangyang, Liu, Guansai, Li, Jin, Ge, Junyou, and Dou, Dengfeng
- Abstract
Peptidyl-prolyl cis–trans isomerase NIMA-interacting 1 (PIN1) has emerged as a promising therapeutic target for cancer treatment. However, the current PIN1 inhibitors have shown limited efficacy in animal models, leaving the question of whether PIN1 is a proper oncologic target still unanswered. By screening a 1 trillion DNA-encoded library (DEL), we identified novel nonacidic compounds. Among resynthesized DEL compounds, DEL1067-56-469(A0) is the most potent one (KD = 430 nM, IC50= 420 nM). Further optimization of A0resulted in compound C10with much improved potency (KD = 25 nM, IC50= 150 nM). As an alternative approach, C10was then converted into proteolysis targeting chimeras (PROTACs) in order to achieve deeper downregulation of the PIN1 protein in cancer cell lines. Unfortunately, neither PIN1 inhibitors nor PIN1 PROTACs demonstrated meaningful antiproliferation activity. In addition, siRNA knock-down experiments provided unfavorable evidence of PIN1 as an oncologic target. Our findings highlight the complexity of targeting PIN1 for cancer therapy.
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- 2024
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4. Regulation of cardiac fibroblasts reprogramming into cardiomyocyte‐like cells with a cocktail of small molecule compounds.
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Chang, Danyang, Sun, Changye, Tian, Xiangqin, Liu, Hongyin, Jia, Yangyang, and Guo, Zhikun
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SMALL molecules ,CALCIUM ions ,FIBROBLASTS ,CARDIAC contraction ,CONNEXIN 43 ,MYOCARDIAL infarction - Abstract
Myocardial infarction results in extensive cardiomyocyte apoptosis, leading to the formation of noncontractile scar tissue. Given the limited regenerative capacity of adult mammalian cardiomyocytes, direct reprogramming of cardiac fibroblasts (CFs) into cardiomyocytes represents a promising therapeutic strategy for myocardial repair, and small molecule drugs might offer a more attractive alternative to gene editing approaches in terms of safety and clinical feasibility. This study aimed to reprogram rat CFs into cardiomyocytes using a small molecular chemical mixture comprising CHIR99021, Valproic acid, Dorsomorphin, SB431542, and Forskolin. Immunofluorescence analysis revealed a significant increase in the expression of cardiomyocyte‐specific markers, including cardiac troponin T (cTnT), Connexin 43 (Cx43), α‐actinin, and Tbx5. Changes in intracellular calcium ion levels and Ca2+ signal transfer between adjacent cells were monitored using a calcium ion fluorescence probe. mRNA sequencing analysis demonstrated the upregulation of genes associated with cardiac morphogenesis, myocardial differentiation, and muscle fiber contraction during CF differentiation induced by the small‐molecule compounds. Conversely, the expression of fibroblast‐related genes was downregulated. These findings suggest that chemical‐induced cell fate conversion of rat CFs into cardiomyocyte‐like cells is feasible, offering a potential therapeutic solution for myocardial injury. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Study on the friction and wear properties of zinc oxide/silicon dioxide composite-coated paper mulch film.
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Li, Anling, Jia, Yangyang, Li, Zhen, Ren, Shuaiyang, Zhang, Fengwei, and He, Qiang
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MECHANICAL wear ,SILICA ,ZINC oxide ,MULCHING ,COMPOSITE coating ,ZINC oxide films - Abstract
Paper mulching films are affected by wind and rain in the field, which lead to the problems of water absorption, shrinkage, and friction with the soil surface. In order to achieve better hydrophobic durability and wear resistance of paper mulching films, a paper mulching film with ZnO/SiO 2 composite coating was prepared by the brush coating method. Taking ceramic balls as friction pairs, reciprocating friction tests were carried out with two kinds of paper mulching films at different temperatures. The morphology of the raw paper mulch film and the ZnO/SiO 2 composite-coated paper mulch film after rubbing at different temperatures and the reason of the hydrophobic durability of the coated paper mulch film were analyzed using a scanning electron microscope and a three-dimensional topography instrument. The results show that ZnO/SiO 2 composite coating can improve the wear resistance and hydrophobic durability of the paper mulch film. Through surface morphology analysis, it can be found that the composite coating plays a role in modification and filling and improves the adhesion between the fibers by reducing the pores on the surface of the paper mulch film, finally improving the wear resistance and hydrophobic durability of the paper mulch film. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Role of circPSEN1 in carbon black and cadmium co-exposure induced autophagy-dependent ferroptosis in respiratory epithelial cells.
- Author
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Mao, Rulin, Yang, Yusi, Zheng, Liting, Liang, Xiaohong, Jia, Yangyang, and Shao, Yueting
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POISONS ,EPITHELIAL cells ,GLUTATHIONE peroxidase ,CADMIUM ,TRANSFERRIN ,MICROTUBULE-associated proteins ,CARBON-black ,TRANSFERRIN receptors ,CIGARETTE smoke - Abstract
Carbon black and cadmium (Cd) are important components of atmospheric particulate matter and cigarette smoke that are closely associated with the occurrence and development of lung diseases. Carbon black, particularly carbon black nanoparticles (CBNPs), can easily adsorbs metals and cause severe lung damage and even cell death. Therefore, this study aimed to explore the mechanisms underlying the combined toxicity of CBNPs and Cd. We found that the combined exposure to CBNPs and Cd promoted significantly greater autophagosome formation and ferroptosis (increased malonaldehyde (MDA), reactive oxygen species (ROS), and divalent iron ions (Fe
2+ ) levels and altered ferroptosis-related proteins) compared with single exposure in both 16HBE cells (human bronchial epithelioid cells) and mouse lung tissues. The levels of ferroptosis proteins, transferrin receptor protein 1 (TFRC) and glutathione peroxidase 4 (GPX4), were restored by CBNPs-Cd exposure following treatment with a 3-MA inhibitor. Additionally, under CBNPs-Cd exposure, circPSEN1 overexpression inhibited increases in the autophagy proteins microtubule-associated protein 1 light chain 3 (LC3II/I) and sequestosome-1 (P62). Moreover, increases in TFRC and Fe2+ , and decreases in GPX4were inhibited. Knockdown of circPSEN1 reversed these effects. circPSEN1 interacts with autophagy-related gene 5 (ATG5) protein and upregulates nuclear receptor coactivator 4 (NCOA4), the co-interacting protein of ATG5, thereby degrading ferritin heavy chain 1 (FTH1) and increasing Fe2+ in 16HBE cells. These results indicated that the combined exposure to CBNPs and Cd promoted the binding of circPSEN1 to ATG5, thereby increasing autophagosome synthesis and ATG5-NCOA4-FTH1 axis activation, ultimately inducing autophagy-dependent ferroptosis in 16HBE cells and mouse lung tissues. This study provides novel insights into the toxic effects of CBNPs and Cd in mixed pollutants. [Display omitted] • Carbon black and cadmium co-exposure induce autophagy-dependent ferroptosis. • CircPSEN1 interacts with ATG5 protein to facilitate autophagosome formation. • CircPSEN1 activates ATG5-NCOA4-FTH1 axis leading to ferroptosis. • Ferroptosis induced by carbon black and cadmium co-exposure may be synergistic effect. [ABSTRACT FROM AUTHOR]- Published
- 2024
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7. Microplastics alter the equilibrium of plant-soil-microbial system: A meta-analysis.
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Jia, Yangyang, Cheng, Zhen, Peng, Yi, and Yang, Guojiang
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MICROPLASTICS ,LOW density polyethylene ,PLASTIC marine debris ,BIODEGRADABLE plastics ,POLYVINYL chloride ,PLANT-soil relationships ,ENVIRONMENTAL security ,POLYETHYLENE - Abstract
Microplastics (MPs) are widely identified as emerging hazards causing considerable eco-toxicity in terrestrial ecosystems, but the impacts differ in different ecosystem functions among different chemical compositions, morphology, sizes, concentrations, and experiment duration. Given the close relationships and trade-offs between plant and soil systems, probing the "whole ecosystem" instead of individual functions must yield novel insights into MPs affecting terrestrial ecosystems. Here, a comprehensive meta-analysis was employed to reveal an unambiguous response of the plant-soil-microbial system to MPs. Results showed that in view of plant, soil, and microbial functions, the general response patterns of plant and soil functions to MPs were obviously opposite. For example, polyethylene (PE) and polyvinyl chloride (PVC) MPs highly increased plant functions, while posed negative effects on soil functions. Polystyrene (PS) and biodegradable (Bio) MPs decreased plant functions, while stimulating soil functions. Additionally, low-density polyethylene (LDPE), PE, PS, PVC, Bio, and granular MPs significantly decreased soil microbial functions. These results clearly revealed that MPs alter the equilibrium of the plant-soil-microbial system. More importantly, our results further revealed that MPs tended to increase ecosystem multifunctionality, e.g., LDPE and PVC MPs posed positive effects on ecosystem multifunctionality, PE, PS, and Bio MPs showed neutral effects on ecosystem multifunctionality. Linear regression analysis showed that under low MPs size (<100 µm), ecosystem multifunctionality was gradually reduced with the increased size of MPs. The response of ecosystem multifunctionality showed a concave shape pattern along the gradient of experimental duration which was lower than 70 days. More importantly, there was a threshold (i.e., 5% w/w) for the effects of MPs concentration on ecosystem multifunctionality, i.e., under low concentration (< 5% w/w), ecosystem multifunctionality was gradually increased with the increased concentration of MPs, while ecosystem multifunctionality was gradually decreased under high concentration (i.e., > 5% w/w). These findings emphasize the importance of studying the effects of MPs on plant-soil-microbial systems and help us identify ways to reduce the eco-toxicity of MPs and maintain environmental safety in view of an ecology perspective. [Display omitted] • MPs posed opposite effects on plant and soil functions. • Soil microbial functions endured more severe damage from MPs. • MPs are likely to cause positive effects on ecosystem multifunctionality. • Impacts of MPs on EMF are type, size, concentration and duration dependency. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Mycorrhizal fungi mitigate nitrogen losses of an experimental grassland by facilitating plant uptake and soil microbial immobilization
- Author
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JIA, Yangyang, VAN DER HEIJDEN, Marcel, VALZANO-HELD, Alain Y., JOCHER, Markus, and WALDER, Florian
- Abstract
Nitrogen (N) is one of the most limited nutrients of terrestrial ecosystems, whose losses are prevented in tightly coupled cycles in finely tuned systems. Global change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer nowadays challenge the terrestrial N cycle. Arbuscular mycorrhizal fungi (AMF) are known drivers of plant-soil nutrient fluxes, yet a comprehensive assessment of AMF involvement in N cycling under global change is still lacking. Here we simulated N enrichment by fertilization in experimental grassland microcosms grown under greenhouse conditions in the presence or absence of AMF and steadily monitored different N pathways over nine months. We found that N enrichment by fertilization decreased the relative abundance of legumes, and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF based on aboveground biomass. AMF always maintained plant N: phosphorus (P) ratio between 14 and 16, no matter changes in the soil N availability. Shifting plant N:P ratios due to the increased plant N and P uptake thus might be a primary pathway of AMF altering plant community composition. Furthermore, we constructed a comprehensive picture of AMFs' role in N cycling, highlighting that AMF reduced N losses primarily by mitigating N leaching, while N2O emissions played a marginal role. AMF reduced N2O emissions directly through the promotion of N2O-consuming denitrifiers. The underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial immobilization. Our results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling, and the integral roles of AMF in N cycle terrestrial ecosystems can buffer the upcoming global changes.
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- 2024
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