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Microalgae are considered an efficient accumulator and promising source of Se for feed additive purposes. This study aimed at investigating, for the first time, the effect of phosphorus limitation on Se accumulation and uptake efficiency in N. oceanica. A range of phosphorus concentrations (0–2470 µM) were tested in either the presence or absence of sodium selenite (0, 5, 30 µM). Se accumulation was increased up to 16-fold and Se uptake efficiency was increased up to 3.6-fold under phosphorus growth-limiting concentrations. N. oceanica was then cultivated in a 1.8 L flat-panel photobioreactor in batch operation under two phosphorus growth-limiting concentrations (250 and 750 µM) where the accumulation of Se in the microalgal biomass, as well as its presence in the spent medium were analysed. This study is the first to investigate the effect of phosphorus limitation for increasing Se accumulation in microalgae, and to prevent the release of Se in wastewater.

Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray-Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. We first examined the efficiency of RNA uptake in multiple pathogenic and non-pathogenic fungi, and an oomycete pathogen. We observed efficient double-stranded RNA (dsRNA) uptake in the fungal plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Aspergillus niger, and Verticillium dahliae, but no uptake in Colletotrichum gloeosporioides, and weak uptake in a beneficial fungus, Trichoderma virens. For the oomycete plant pathogen, Phytophthora infestans, RNA uptake was limited, and varied across different cell types and developmental stages. Topical application of dsRNA targeting virulence-related genes in the pathogens with high RNA uptake efficiency significantly inhibited plant disease symptoms, whereas the application of dsRNA in pathogens with low RNA uptake efficiency did not suppress infection. Our results have revealed that dsRNA uptake efficiencies vary across eukaryotic microbe species and cell types. The success of SIGS for plant disease management can largely be determined by the pathogen RNA uptake efficiency.

This study aims to investigate radiolabeled Cefpodoxime Proxetil loaded chitosan (CP–CS) nanoparticles as nuclear imaging infection agent to Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). The encapsulation efficiency of Cefpodoxime Proxetil was found 82 ± 2%. CP and CP–CS nanoparticles were radiolabeled with Tc-99 m. The radiochemical purity of 99mTc–CP and 99mTc–CP–CS nanoparticles were determined by RTLC as 89 ± 3% and 94 ± 2% respectively. In vitro bindings of 99mTc–CP–CS nanoparticles to S. aureus and E. coli were found higher than 99mTc–CP bindings. © 2020, Akadémiai Kiadó, Budapest, Hungary.

Continuous nitrogen (N) deposition has raised concerns that temperate forest ecosystems shift from N limitation to progressing phosphorus (P) limitation under global change. According to the multiple resource limitation theory, this will not only influence P economy, but also reduce N uptake and use efficiencies of trees such that growth is equally limited by N and P. We used different global change scenarios including summer drought to test the prediction of this hypothesis for the N economy of European beech (Fagus sylvatica L.) saplings. Our study demonstrates that the N uptake efficiency (NUptakeE) of beech adjusts to soil N availability and stabilizes leaf and fine root N concentrations. By contrast, both efficiencies of N use were curtailed by increasing P limitation under elevated soil N. The photosynthetic N use efficiency (PNUE) was serially reduced with decreasing soil P availability and increasing foliar N:P ratios, while the N use efficiency (NUE) decreased with increasing fine root N:P ratios. Soil drought induced relative P deficiency alike and reduced NUptakeE, PNUE, and NUE independent from the soil N:P ratios. We conclude that not only N deposition but also increasing summer droughts might affect N:P ratios, thereby inducing P imbalances and affecting the N economy of European beech saplings under global change.

While a severe decrease in phosphorus (P) availability is already taking place in a large number of ecosystems, drought and nitrogen (N) deposition will likely further decrease the availability of P under global change. Plants have developed physiological strategies to cope with decreasing P resources, but it is unclear how these strategies respond to elevated N deposition and summer droughts. We investigated the influence of N and P availability and soil drought on P uptake (H333PO4 feeding experiment) and use efficiencies in young Quercus calliprinos Webb. trees. We hypothesized that (H1) the expected increases in soil N:P ratios will increase the efficiencies of P uptake and use of oak saplings but will decrease the efficiencies of N uptake and use, whereas (H2) drought will affect P uptake efficiency more than N uptake efficiency. In confirmation of (H1) we found that a sharp increase of the soil N:P ratio from 4 to 42 g g-1 significantly increased the instantaneous 33P uptake efficiency (33PUptakeE) by five-fold and long-term P uptake efficiency (PUptakeE) by six-fold, while it decreased N uptake efficiency (NUptakeE) and N use efficiency (NUE). In contradiction to (H1), P use efficiency (PUE) did not respond to the simulated extended gradient of soil N:P ratios but remained relatively constant. (H2) was only partially confirmed as soil drought reduced PUptakeE by up to a fourth at high soil N:P ratios but had no significant effect on NUptakeE. As a consequence, increasing summer droughts may decrease the response of PUptakeE to increasing P limitation, which – in the absence of adjustments of the efficiency of P use – can aggravate growth reductions in this eastern Mediterranean tree species under global change. Open-Access-Publikationsfonds 2019 peerReviewed

Submitted by Camila Baptista do Amaral (camila.agro07@gmail.com) on 2018-04-10T02:12:53Z No. of bitstreams: 1 Tese_completa_FF.pdf: 1170751 bytes, checksum: 2e9767421fbf6acfef2ea2d84ef19a67 (MD5) Approved for entry into archive by Alexandra Maria Donadon Lusser Segali null (alexmar@fcav.unesp.br) on 2018-04-10T11:30:45Z (GMT) No. of bitstreams: 1 amaral_cb_dr_jabo.pdf: 1170751 bytes, checksum: 2e9767421fbf6acfef2ea2d84ef19a67 (MD5) Made available in DSpace on 2018-04-10T11:30:45Z (GMT). No. of bitstreams: 1 amaral_cb_dr_jabo.pdf: 1170751 bytes, checksum: 2e9767421fbf6acfef2ea2d84ef19a67 (MD5) Previous issue date: 2018-03-22 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Altas doses de nitrogênio são necessárias para atingir elevadas produtividades na cultura do milho, e o uso de genótipos eficientes no uso de nitrogênio permitiria atingir tais produtividades utilizando menor quantidade de fertilizantes nitrogenados. O objetivo do trabalho foi caracterizar sintéticos de milho quanto à eficiência de uso de nitrogênio e estudar as correlações entre os componentes da eficiência. Para caracterizar os sintéticos a campo, foram conduzidos experimentos na safra 2015/2016 e 2016/2017. Doze sintéticos e duas testemunhas comerciais (AL Avaré e Ipanema) foram cultivadas sob alta e baixa disponibilidade de nitrogênio em cada uma das safras, totalizando quatro experimentos. Sob baixa disponibilidade de nitrogênio, apenas 24 kg ha-1 do elemento foram aplicados na semeadura, enquanto que sob alta disponibilidade, a adubação nitrogenada foi constituída de 24 kg ha-1 na semeadura e 120 kg ha-1 em cobertura no estádio V4-5. Os experimentos foram conduzidos no delineamento de blocos ao acaso, e foram avaliados o índice do conteúdo de clorofila, acamamento, prolificidade e produtividade de grãos. A disponibilidade de nitrogênio alterou o índice do conteúdo de clorofila dos sintéticos, sendo que maiores valores foram observados sob alta disponibilidade de nitrogênio. Todas os sintéticos foram considerados adequados para cultivo sob baixa disponibilidade de nitrogênio, pois não houve diferença entre alta e baixa disponibilidade para os caracteres acamamento, prolificidade e produtividade de grãos. Considerando todos os caracteres estudados, dois sintéticos foram identificados como superiores, por apresentarem altos valores de índice de conteúdo de clorofila, resistência ao acamamento, prolificidade e produtividade de grãos, com desempenho superior às testemunhas. A fim de estudar os caracteres relacionados com a eficiência de uso de nitrogênio, dois sintéticos e uma testemunha, AL Avaré, foram cultivados em vasos, sob níveis contrastantes de disponibilidade de nitrogênio. Sob baixa disponibilidade, foram aplicados 100 mg dm-3 de nitrogênio em cada vaso na semeadura, e sob alta disponibilidade, foram aplicados 100 mg dm-3 por vaso na semeadura, 150 mg dm-3 em V3 e 150 mg dm-3 em V5. O experimento foi conduzido no delineamento de blocos ao acaso, em esquema fatorial 3x2. Foram avaliados o índice do conteúdo de clorofila, massa seca da parte aérea, massa seca da raiz, eficiência de absorção, eficiência de utilização e eficiência de uso de nitrogênio. O índice de conteúdo de clorofila diferiu entre os níveis contrastantes de nitrogênio, mas não entre os sintéticos. A massa seca da parte aérea foi diminuída pela baixa disponibilidade de nitrogênio, enquanto a resposta dos sintéticos para massa seca da raiz foi variável em função da disponibilidade de nitrogênio. Houve correlação forte e positiva entre eficiência de absorção e eficiência de uso de nitrogênio, sendo que a eficiência de absorção foi correlacionada com a massa seca das raízes. High doses of nitrogen are necessary to reach high yields in maize crop, and the use of genotypes efficient in the use of nitrogen would allow to reach such productivities using less amount of nitrogen fertilizers. The objective of this work was to characterize maize synthetics as to nitrogen use efficiency and to study the correlations between the efficiency components. To characterize the synthetic in a field level, experiments were conducted in 2015/2016 and 2016/2017 seasons. Twelve synthetics and two commercial control (AL Avaré and Ipanema) were cultivated under high and low nitrogen availability in each of the seasons, totalizing four experiments. Under low nitrogen availability, only 24 kg ha-1 of the element were applied at sowing, while under high availability, nitrogen fertilization consisted of 24 kg ha-1 at sowing and 120 kg ha-1 topdressed at the V4-5. The experiments were conducted in a randomized complete block design, and the chlorophyll content index, lodging, prolificacy and grain yield were evaluated. The nitrogen availability altered the chlorophyll content index of the synthetic, and higher values were observed under high nitrogen availability. All the synthetics were considered suitable for cultivation under low nitrogen availability, since there was no difference between high and low availability for lodging, prolificacy and grain yield. Considering all the traits studied, two synthetics were identified as superior, because they presented high values of chlorophyll content index, lodging resistance, prolificacy and grain yield, with superior performance to the control. In order to study the traits related to nitrogen use efficiency, two synthetics and one control, AL Avaré, were grown in pots, under contrasting levels of nitrogen availability. Under low availability, 100 mg dm-3 of nitrogen was applied to each pot at sowing, and under high availability, 100 mg dm-3 per pot was applied at sowing, 150 mg dm-3 at V3 and 150 mg dm-3 at V5. The experiment was conducted in a randomized complete block design, in a 3x2 factorial scheme. The chlorophyll content index, shoot dry mass, root dry mass, uptake efficiency, utilization efficiency and nitrogen use efficiency were evaluated. The chlorophyll content index differed between the contrasting levels of nitrogen, but not among the synthetics. The shoot dry mass was diminished by the low nitrogen availability, while the response of the synthetics to root dry mass was variable as a function of the nitrogen availability. There was a strong and positive correlation between uptake efficiency and nitrogen use efficiency, and the uptake efficiency was correlated with the root dry mass.

The effects of different concentrations of P and N, added separately or combined, on the Cr(III) accumulation capacity of P. stratiotes were studied. Plants and pond water with the addition of contaminant(s) were placed in plastic aquaria. Cr concentration was 5 mg L-1, while P and N concentrations were 5 mg L-1 or 10 mg L-1. Nutrient addition significantly favoured Cr removal and enhanced Cr translocation to leaves. In Cr treatments a high detritus formation from loss of root biomass was observed probably due to its toxicity. Cr was mainly accumulated in the detrital fraction, whereas P and N were retained fundamentally in leaves. A toxic effect was observed in the Cr+P10 and Cr+N10 treatments. These results could be applied to enhance Cr removal efficiency of constructed wetlands using P. stratiotes, where nutrient enrichment could be attained by treating sewage together with the industrial effluents. Fil: Di Luca, Gisela Alfonsina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Hadad, Hernán Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analítica; Argentina Fil: Mufarrege, María de Las Mercedes. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Maine, Maria Alejandra. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Sanchez, Gabriela Cristina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina

ilustraciones, fotografías, gráficas, tablas La presente investigación estudia la dinámica del cadmio (Cd) en el trópico ácido en presencia de hongos formadores de micorrizas arbusculares (HFMA) cuando se tienen suelos con concentraciones del metal, destinados para cultivo de cacao. Para lograr este objetivo se examina la distribución del Cd en las fracciones del suelo y en los diferentes órganos de la planta. Se concluye que, principalmente, a altas concentraciones del metal en el suelo, cuando la fuente del mismo es antrópica, el hongo puede modificar la dinámica del mismo, su disponibilidad en el tiempo. Adicionalmente, por medio del análisis de parámetros fisiológicos y de producción de biomasa sobre las plantas se encuentra que dependiendo del nivel de cadmio y para las concentraciones más altas del metal, el hongo tiene un efecto benéfico sobre el funcionamiento del aparato fotosintético, permitiendo a las plantas una ventaja sobre las plantas no inoculadas contra los efectos tóxicos del metal pesado. Se estudió adicionalmente la capacidad de absorción, translocación y fitoextracción de las plantas inoculadas comparadas con las no inoculadas, de lo que se concluye que para concentraciones altas de cadmio en el suelo la Eficiencia de Absorción (E abs) es mayor en plantas inoculadas, al igual que la Eficiencia de translocación (E transl) del metal, mientras que la Eficiencia de la fitoextracción (E fitoext) tiende a disminuir. (Texto tomado de la fuente). This research studies the dynamics of cadmium in a tropical acidic soil when it is contaminated with different metal concentration and in the presence of Arbuscular Mycorrhizal fungi (AMF), in cocoa cropping. In order to achieve this goal we´ve looked for cadmium distribution in different soils fractions and in different plant organs, concluding that, at high soil cadmium concentration, when the origin of cadmium contamination is antropogenic, the AMF can modify the metal dynamics, changing its bioavailability along. Aditionally, the physiological parameters and biomass plants production depended on the cadmium level. For the higher soil cadmium concentrations, the AMF exerted a beneficial effects, on the photosynthetic apparatus performance, giving inoculated plants an advantage over the non-inoculated plants against the toxic heavy metal´s effects. The uptake, translocation and fitoextraction plant´s ability was calculated for inoculated plants compared to non-inoculated plants. We concluded that at high soil cadmium concentration, the E uptake is better in inoculated plants, the same was observed for E translocation, while an opposite effect was observed for E fitoextraction. Maestría Magíster en Ciencias Agrarias Suelos y aguas Ciencias Agronómicas

Faba bean (Vicia faba L.) is one of the most important food legumes grown in Morocco under rainfed conditions. This crop requires phosphorus (P) fertilizers to produce higher yields. However, many farmers use low quantities of P because of its cost and the risk of drought. Consequently, the use of varieties with high P use efficiency (PUE) can improve productivity and farmers' income under erratic conditions where the application of high amounts of P is not economically justified. The genetic variation of PUE among Moroccan faba bean varieties was studied under different levels of available phosphorus to orientate the choice of adapted varieties. Two experiments were conducted, under rainfed conditions, at the experiment station of Douyet (Morroco) in 2000-2001 (year 1) and 2002-2003 (year 2). In both years, four P treatments 0, 40, 80 and 120 kg P 2O 5 ha -1 as fertilizer and four faba bean varieties (G) were tested. In year 1, the varieties tested were Aguadulce, Defès, Karabiga and Lobab. The same varieties were used in year 2 with the exception of Aguadulce, which was replaced by a determinate genotype with smaller straw production. Results showed that the G effect on PUE was significant in year 1, but not in year 2. In the conditions of the moderate P availability (year 1), the variety Defes had the highest PUE and grain yield. Where P availability was lower (year 2), the highest PUE was reached by Karabiga; however, the difference with Defes was not significant. Data showed also that PUE was positively correlated with harvest index (HI) and phosphorus harvest index (PHI). In year 1, genotypic variation for PUE was mainly explained by phosphorus utilization efficiency; but not by phosphorus uptake efficiency. From this study, we can conclude that for rainfed conditions, the breeding strategy is to develop genotypes like Defes with high HI and improved PUE. © 2012 Copyright Taylor and Francis Group, LLC.

Embora não seja considerado nutriente essencial às plantas, o Si é classificado como elemento benéfico ou útil, mas pode alterar a dinâmica nutricional das plantas. Objetivando comparar a eficiência nutricional de variedades de cafeeiro (Catuaí, Mundo Novo e Icatu) em resposta à adubação silicatada, realizou-se um experimento em blocos casualizados, com as três variedades de mudas em tubetes combinadas com seis doses de CaSiO3 (T0 = 0, T1 = 0,063, T2 = 0,125, T3 = 0,25, T4 = 0,5 e T5 = 1,0 g dm-3 de substrato). A variedade Icatu teve a maior eficiência de absorção (EA) de Cu, Zn, Fe e Si, maior eficiência de uso (EU) de N, K, Ca, B e Mn, não diferindo da Mundo Novo com relação aos nutrientes N, Ca e Mn, e maior eficiência de translocação (ET) de N, S, Zn e de Fe. A Catuaí teve maior EA de P, K, B e Mn, não diferindo da Mundo Novo com relação aos nutrientes P, K e Mn, maior EU para Mg, S, Cu, Zn, Fe e Si, provavelmente devido à melhor ET desses nutrientes, exceto para Fe e Si. A Mundo Novo foi mais eficiente na absorção de N, K, Ca, Mg, Mn e Si, teve maior EU de P e Mn e maior ET de K, Ca, B, Mn e Si.Silicon is classified as a beneficial nutrient and can improve the nutritional dynamics of plants, although it is not considered an essential nutrient for plants. In an experiment in a randomized block design, three coffee cultivars (Catuaí, Mundo Novo and Icatu) were planted in plastic tubes, combined with six doses of calcium silicate (T0=0, T1=0.063, T2=0.125, T3=0.25, T4=0.5 and T5=1.0 g dm-3 substrate), to compare the nutritional efficiency of the cultivars in response to silicon fertilization. Cultivar Icatu showed the higher uptake efficiency (UE) of Cu, Zn, Fe, and Si, and efficiency of use (EU) of N, K, Ca, B, and Mn, but the later did not differ from Mundo Novo cultivar (N, Ca, and Mn). The efficiency of translocation (ET) was higher for N, S, Zn, and Fe. For Catuaí the uptake efficiency of P, K, B, and Mn was higher, but did not differ from Mundo Novo for P, K, and Mn. The Catuai's s efficiency of use for Mg, S, Cu, Zn, Fe, and Si was higher, probably because of a better ET of these nutrients, with exception of Fe and Si. The cultivar Mundo Novo was the most efficient in N, K, Ca, Mg, Mn and Si uptake, had the higher EU of P and Mn, and the highest ET of K, Ca, B, Mn, and Si.

The aim of this work was to compare Cr(III) and Cr(VI) removal kinetics from water by Pistia stratiotes and Salvinia herzogii. The accumulation in plant tissues and the effects of both Cr forms on plant growth were also evaluated. Plants were exposed to 2 and 6 mg L−1 of Cr(III) or Cr(VI) during 30 days. At the end of the experiment, Cr(VI) removal percentages were significantly lower than those obtained for Cr(III) for both macrophytes. Cr(III) removal kinetics involved a fast and a slow component. The fast component was primarily responsible for Cr(III) removal while Cr(VI) removal kinetics involved only a slow process. Cr accumulated principally in the roots. In the Cr(VI) treatments a higher translocation from roots to aerial parts than in Cr(III) treatments was observed. Both macrophytes demonstrated a high ability to remove Cr(III) but not Cr(VI). Cr(III) inhibited the growth at the highest studied concentration of both macrophytes while Cr(VI) caused senescence. These results have important implications in the use of constructed wetlands for secondary industrial wastewater treatment. Common primary treatments of effluents containing Cr(VI) consists in its reduction to Cr(III). Cr(III) concentrations in these effluents are normally below the highest studied concentrations in this work. Fil: Maine, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina Fil: Hadad, Hernán Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina Fil: Sánchez, Gabriela Cristina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina Fil: Caffaratti, Sandra Ester. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina Fil: Pedro, María del Carmen. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina

O objetivo deste trabalho foi caracterizar cultivares brasileiras de trigo, desenvolvidas em diferentes décadas, quanto à eficiência de uso de N, sob diferentes disponibilidades do nutriente. Dez cultivares de trigo, lançadas entre 1940 e 2009, foram submetidas a quatro doses de adubação nitrogenada (0, 60, 120 e 180 kg ha-1 de N), em ambiente controlado, em delineamento de blocos ao acaso, com três repetições. A produtividade de grãos aumentou em 0,54, 0,74, 0,74 e 0,82% ao ano, nas doses de 0, 60, 120 e 180 kg ha-1 de N, respectivamente. As eficiências de absorção e de utilização de N estiveram positivamente associadas à eficiência de uso do nitrogênio, com estreitamento na relação em condições de alta disponibilidade de N. As cultivares modernas são mais eficientes no uso do N e toleram doses mais baixas do nutriente, em comparação às cultivares pioneiras. Para o desenvolvimento de cultivares mais eficientes no uso de N, os programas de melhoramento genético de trigo devem priorizar a seleção de genótipos com maiores eficiências de absorção, remobilização e utilização de N. The objective of this work was to characterize Brazilian wheat cultivars, developed in different decades, as to their nitrogen use efficiency, under different availabilities of the nutrient. Ten wheat cultivars, released between 1940 and 2009, were subjected to four nitrogen fertilization rates (0, 60, 120, and 180 kg ha-1 N), under a controlled environment, in a randomized complete block design, with three replicates. Grain yield increased in 0.54, 0.74, 0.74, and 0.82% per year at the rates of 0, 60, 120, and 180 kg ha-1 N, respectively. Nitrogen uptake and utilization efficiencies were positively associated with N use efficiency, with strengthening relation under high N availability conditions. Modern cultivars are more efficient in N use and tolerate lower rates of the nutrient in comparison to pioneer cultivars. For the development of more efficient cultivars in N use, wheat-breeding programs should prioritize the selection of genotypes with higher N uptake, remobilization, and utilization efficiencies.

Silicon is considered an important chemical element for rice, because it can improve tolerance to biotic and abiotic stress. However, in many situations no positive effect of silicon was observed, probably due to genetic factors. The objective of this research was to monitor Si uptake kinetics and identify responses of rice cultivars in terms of Si uptake capacity and use. The experiment was carried out in a greenhouse of the São Paulo State University (UNESP), Brazil. The experiment was arranged in a completely randomized, factorial design with three replications. that consisted of two rice cultivars and two Si levels. Kinetic parameters (Vmax, Km, and Cmin), root morphology variables, dry matter yield, Si accumulation and levels in shoots and roots, uptake efficiency, utilization efficiency, and root/shoot ratio were evaluated. Higher Si concentrations in the nutrient solution did not increase rice dry matter. The development of the low-affinity silicon uptake system of the rice cultivar 'Caiapó' was better than of 'Maravilha'. O silício é considerado um elemento químico importante para o arroz, pois tem proporcionado tolerância a estresses bióticos e abióticos. No entanto, em muitas situações, não tem sido constatado efeito positivo desse mineral, o que pode estar relacionado a fatores genéticos. Este trabalho teve por objetivo encontrar respostas entre cultivares de arroz quanto à capacidade de absorção e utilização do Si. O experimento foi desenvolvido em casa de vegetação, na Faculdade de Ciências Agronômicas/Unesp. O delineamento experimental foi inteiramente casualizado, composto por dois cultivares e dois níveis de Si, com seis repetições. Foram avaliados os parâmetros cinéticos (Vmax, Km e Cmin), as características morfológicas de raízes, a massa de matéria seca, o teor e quantidade de Si acumulada, a eficiência de absorção e utilização e a relação raiz/parte aérea. A maior concentração de Si na solução nutritiva não promoveu aumento da massa de matéria seca do arroz. O cultivar Caiapó mostrou maior desenvolvimento do sistema de absorção de Si de baixa afinidade, em relação ao Maravilha.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:46:20Z No. of bitstreams: 1 S0100-06832012000100016.pdf: 769098 bytes, checksum: 17b49139a8e5a68ef69005ef1c0601e5 (MD5) Made available in DSpace on 2013-08-22T18:46:20Z (GMT). No. of bitstreams: 1 S0100-06832012000100016.pdf: 769098 bytes, checksum: 17b49139a8e5a68ef69005ef1c0601e5 (MD5) Previous issue date: 2012-02-01 Made available in DSpace on 2013-09-30T19:35:34Z (GMT). No. of bitstreams: 2 S0100-06832012000100016.pdf: 769098 bytes, checksum: 17b49139a8e5a68ef69005ef1c0601e5 (MD5) S0100-06832012000100016.pdf.txt: 41291 bytes, checksum: 587e65209ad35e373bbc31ec231d9c64 (MD5) Previous issue date: 2012-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:09:34Z No. of bitstreams: 2 S0100-06832012000100016.pdf: 769098 bytes, checksum: 17b49139a8e5a68ef69005ef1c0601e5 (MD5) S0100-06832012000100016.pdf.txt: 41291 bytes, checksum: 587e65209ad35e373bbc31ec231d9c64 (MD5) Made available in DSpace on 2014-05-20T15:09:34Z (GMT). No. of bitstreams: 2 S0100-06832012000100016.pdf: 769098 bytes, checksum: 17b49139a8e5a68ef69005ef1c0601e5 (MD5) S0100-06832012000100016.pdf.txt: 41291 bytes, checksum: 587e65209ad35e373bbc31ec231d9c64 (MD5) Previous issue date: 2012-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O silício é considerado um elemento químico importante para o arroz, pois tem proporcionado tolerância a estresses bióticos e abióticos. No entanto, em muitas situações, não tem sido constatado efeito positivo desse mineral, o que pode estar relacionado a fatores genéticos. Este trabalho teve por objetivo encontrar respostas entre cultivares de arroz quanto à capacidade de absorção e utilização do Si. O experimento foi desenvolvido em casa de vegetação, na Faculdade de Ciências Agronômicas/Unesp. O delineamento experimental foi inteiramente casualizado, composto por dois cultivares e dois níveis de Si, com seis repetições. Foram avaliados os parâmetros cinéticos (Vmax, Km e Cmin), as características morfológicas de raízes, a massa de matéria seca, o teor e quantidade de Si acumulada, a eficiência de absorção e utilização e a relação raiz/parte aérea. A maior concentração de Si na solução nutritiva não promoveu aumento da massa de matéria seca do arroz. O cultivar Caiapó mostrou maior desenvolvimento do sistema de absorção de Si de baixa afinidade, em relação ao Maravilha. Silicon is considered an important chemical element for rice, because it can improve tolerance to biotic and abiotic stress. However, in many situations no positive effect of silicon was observed, probably due to genetic factors. The objective of this research was to monitor Si uptake kinetics and identify responses of rice cultivars in terms of Si uptake capacity and use. The experiment was carried out in a greenhouse of the São Paulo State University (UNESP), Brazil. The experiment was arranged in a completely randomized, factorial design with three replications. that consisted of two rice cultivars and two Si levels. Kinetic parameters (Vmax, Km, and Cmin), root morphology variables, dry matter yield, Si accumulation and levels in shoots and roots, uptake efficiency, utilization efficiency, and root/shoot ratio were evaluated. Higher Si concentrations in the nutrient solution did not increase rice dry matter. The development of the low-affinity silicon uptake system of the rice cultivar 'Caiapó' was better than of 'Maravilha'. UNESP College of Agricultural Sciences Department of Crop Science UNESP College of Agricultural Sciences Department of Crop Science

Approximately 30% of Florida citrus is grown on well-drained Entisols with low nutrient-holding capacity, which are prone to high nitrogen (N) leaching losses. However, increasing application frequency of N-fertilizer via multiple fertigations does not increase crop yield, whereas in agronomic crops, such an approach typically enhances N uptake efficiency. We assessed seasonal tree N tissue concentration dynamics as affected by N rate for mature fourteen-year-old 'Hamlin' orange (Citrus sinensis L. Osbeck) trees on either Carrizo citrange (C. sinsensis L. Osbeck X Poncirus trifoliata L. Raf.) or Swingle citrumelo (C. paradisi Macf. X P. trifoliata L. Raf.) rootstocks. Nitrogen was applied as ammonium nitrate in six split fertigation applications with N target values of 179 and 269 kg ha(-1)yr(-1). Leaf, twig, and branch bark tissue N concentrations decreased through the spring to minima in May and June. This time period corresponds to a period of high N demand associated with both vegetative and reproductive growth. Tissue N concentrations increased from late spring minimums to fall and winter maximum concentrations. Reduction in branch bark and wood tissue N concentrations may have been due to a redistribution of N to leaf, twig, and fruit tissues in response to low N supply. The majority of the spring N should be supplied prior to May.

Embora não seja considerado nutriente essencial às plantas, o Si é classificado como elemento benéfico ou útil, mas pode alterar a dinâmica nutricional das plantas. Objetivando comparar a eficiência nutricional de variedades de cafeeiro (Catuaí, Mundo Novo e Icatu) em resposta à adubação silicatada, realizou-se um experimento em blocos casualizados, com as três variedades de mudas em tubetes combinadas com seis doses de CaSiO3 (T0 = 0, T1 = 0,063, T2 = 0,125, T3 = 0,25, T4 = 0,5 e T5 = 1,0 g dm-3 de substrato). A variedade Icatu teve a maior eficiência de absorção (EA) de Cu, Zn, Fe e Si, maior eficiência de uso (EU) de N, K, Ca, B e Mn, não diferindo da Mundo Novo com relação aos nutrientes N, Ca e Mn, e maior eficiência de translocação (ET) de N, S, Zn e de Fe. A Catuaí teve maior EA de P, K, B e Mn, não diferindo da Mundo Novo com relação aos nutrientes P, K e Mn, maior EU para Mg, S, Cu, Zn, Fe e Si, provavelmente devido à melhor ET desses nutrientes, exceto para Fe e Si. A Mundo Novo foi mais eficiente na absorção de N, K, Ca, Mg, Mn e Si, teve maior EU de P e Mn e maior ET de K, Ca, B, Mn e Si. Silicon is classified as a beneficial nutrient and can improve the nutritional dynamics of plants, although it is not considered an essential nutrient for plants. In an experiment in a randomized block design, three coffee cultivars (Catuaí, Mundo Novo and Icatu) were planted in plastic tubes, combined with six doses of calcium silicate (T0=0, T1=0.063, T2=0.125, T3=0.25, T4=0.5 and T5=1.0 g dm-3 substrate), to compare the nutritional efficiency of the cultivars in response to silicon fertilization. Cultivar Icatu showed the higher uptake efficiency (UE) of Cu, Zn, Fe, and Si, and efficiency of use (EU) of N, K, Ca, B, and Mn, but the later did not differ from Mundo Novo cultivar (N, Ca, and Mn). The efficiency of translocation (ET) was higher for N, S, Zn, and Fe. For Catuaí the uptake efficiency of P, K, B, and Mn was higher, but did not differ from Mundo Novo for P, K, and Mn. The Catuai's s efficiency of use for Mg, S, Cu, Zn, Fe, and Si was higher, probably because of a better ET of these nutrients, with exception of Fe and Si. The cultivar Mundo Novo was the most efficient in N, K, Ca, Mg, Mn and Si uptake, had the higher EU of P and Mn, and the highest ET of K, Ca, B, Mn, and Si.

Establishment of nutrient management of citrus groves for high yield and superior crop quality is a need for efficient production. Limited phosphorus (P) availability of low fertility tropical soils in Brazil impairs citrus production since adequate P supply is important in the first years after field planting. Research has demonstrated that tree growth and fruit yield in response to P fertilization vary depending on rootstock varieties. Therefore, this study evaluated P uptake and redistribution by Pêra sweet orange on Rangpur lime or Cleopatra mandarin rootstocks initially grown in nutrient solution containing either deficient (0.005 mmol/L) or suficient(1.0 mmol/L) P levels. Each of these groups of plants was later half maintained in the deficient P whereas other half was changed to the suficient P solutions both labeled with 32P (14.8 MBq per 9.0 L pot). Plants were further grown for 50 days. Based on concentrations of P in plant parts derived from solution and P remobilized in plant, we observed that, despite variations on plant growth on different rootstocks, P taken up by those on Rangpur lime was superior confirming greater efficiency of nutrient uptake of this rootstock under a non mycorrhized condition. Estimates of P remobilized from plant reserve to young leaves suggested that the process was more marked for plants on Cleopatra mandarin irrespective to P treatments. These explained the fact that leaf visual symptoms of P deficiency are more frequent on trees on Cleopatra rootstock and presented the need to fine tune P fertilization recommendations.

Background: Cancer is the leading cause of death in human disease and is a major public health problem around the world. Exosomes are a promising cancer biomarker and therapy target. Recent evidence demonstrate that tumor cells could inhibit natural killer (NK) cells’ immune surveillance function by releasing exosomes into tumor microenvironment. The intercelluar uptake of tumor cell-derived exosomes by NK cells is vital for using these exosomes in tumor diagnose and therapy. We aimed to investigate the efficiency of NK cell uptake of tumor exosomes. Methods: Exosomes derived from different tumor cells, RAW264.7 cells and NK cells were labeled by fluorescent dye and co-cultured with NK cells. The uptake rates of NK cells were observed by fluorescence microscope and analyzed by flow cytometry. Results: NK cells could take up more exosomes from themselves and cell lines originating from bone marrow. Epithelial cell lines can take up more exosomes from epithelial cells. There was no significant difference in uptake efficiency between Jurkat cells and RAW264.7 cells by NK cells, indicating that maybe the origin other than species affects the efficiency of recipient cell uptake of exosomes. Different tumor cells derived exosomes had different uptake efficiency by NK cells. Conclusion: There is certain pattern of NK cells uptake tumor exosomes, which provide important insights on how tumors affect NK cells and develop appropriate countermeasures. In addition, it can be also helpful to select and design proper exosomes as a drug carrier in future.