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Thiamethoxam (TMX) exerts pronounced insecticidal effects against a wide variety of economically imperative pests. However, the administration of TMX in experimental animals induced notable adverse effects on the function of various organs. The purpose of this study was to assess TMX induced hematological, biochemical, and genetic alterations and the potential ameliorative effects on them of Moringa oleifera leaf extract (MLE) in male mice Animals were orally administered TMX (≈1/10 LD50) daily either alone or with MLE (200 mg/kg b.w.) for 28 successive days. Blood was collected to evaluate the hematological profile and serum levels of Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, creatinine, uric acid, and urea. Liver and kidney cells were used to assess the Malondialdehyde (MDA) content and antioxidant enzymes. DNA integrity was estimated also in the liver and kidney using comet and colorimetric diphenylamine assays. Results revealed that TMX exhibited significant changes in the hematological profile and liver and kidney functions. Besides, TMX significantly raised the MDA content and DNA damage in both two of these organs. In contrast, TMX reduced the antioxidant activities in the cells of both liver and kidney. Meanwhile, Moringa extract combined with TMX significantly attenuated the deleterious findings of TMX. Specifically, it improved the TMX-induced hematological changes, liver and kidney function alterations, oxidative stress, and DNA damage rate. It can be concluded that TMX had adverse effects on different cells of male mice, but MLE successfully ameliorated TMX’s hematological and hepatorenal toxicity.

Background & Aims: Systemic lupus erythematous (SLE) is a chronic autoimmune disease which affects females more than males. This study was aimed with the objectives to correlate the patterns and clinical characteristics of Systemic Lupus Erythematous patients. Materials and Methods: This was an observational cross-sectional study conducted at a tertiary care centre in Western Odisha. Patients who fulfilled the 1997 ACR (American College of Rheumatology) classification revised criteria of SLE were enrolled in the study from 24/02/19 to 26/2/21. After history & clinical examination these patients were subjected to various diagnostic procedures so as to confirm the diagnosis of SLE. The clinical and immunological profile of all these patients was mapped to have better understanding of the disease pattern. Results: Over a period of 2 years, a total number of 40 SLE patients were enrolled with a mean age of 30 years. Gender distribution was found to be all female patients. Patients had involvement of various systems like mucocutaneous (70%), renal (67.5%),musculoskeletal (52.5%), neurological(7.5%). The patients were positive for antibodies against dsDNA (47.5%), Ro (42.5%), nRNP (37.5%), nucleosomes (32.5%) followed by ribosomal P-protein (30%), Sm (30%), and histones (25%). Conclusion: The observations of present study suggests that SLE patients may present with a wide variety of clinical manifestations. The disease is more common in females especially during the second and third decade of life. Autoantibody profiling aids in supporting diagnosis. Vigilant evaluation of clinical pattern is required to diagnose the disease and for better treatment.

The objective of this study was to evaluate the effect of supplementation with bee pollen (BP) and propolis (PRO) on productive performance, Eimeria oocyst counts in feces, blood metabolites, and the meat quality of growing rabbits. A total of 160 hybrid rabbits (California × New Zealand) of 30 days of age and 643 ± 8.0 g body weight (BW) were assigned to four treatments with 10 replicates each (four rabbits/replicate). The treatments were as follows: (1) CON: rabbits fed basal diet and not supplemented with BP or PRO; (2) BP500: CON + BP (500 mg/kg BW); (3) PRO50: CON + PRO (50 µL/kg BW); and (4) BP + PRO: CON + BP (500 mg/kg BW) + PRO (50 µL/kg BW). Higher daily weight gain (p = 0.04) and lower feed conversion rate (p = 0.03) were observed in rabbits supplemented with PRO50. In addition, supplementation with PRO50 and BP + PRO reduced the amount of Eimeria oocysts per gram of feces (p < 0.05). Most hematological and serum biochemical parameters were similar in rabbits of all treatments. Protein content, collagen, and meat color were similar between treatments. In conclusion, propolis supplementation (50 µL/kg BW) can prevent coccidiosis and act as a natural growth promoter in rabbits without affecting animal health and meat quality.

The thyroid gland plays a significant role in the metabolism and proliferation of blood cells, hematological disorders are frequently observed in patients with thyroid disorders, and the most frequent problem is anemia. The main objective of this research work is to evaluate the prevalence and types of thyroid dysfunction and their association with anemia in different gender stratified by age in the Asir region of Saudi Arabia. Four years of data from July 2016 to July 2020 for 9992 study subjects were collected. Statistical analysis was performed based on thyroid disorder and anemia stratified by gender and age subgroup. The mean age of the study subject was 43.4 ± 15.8 years, and females constituted 61.7% of cases. Thyroid dysfunction was observed in 49.76% (4973), and subclinical hypothyroidism was the most prevalent type (3922/9992), followed by primary hypothyroidism (530/9992). Females have a significantly higher overall prevalence of thyroid dysfunction than males (p &lt, 0.05). Anemia was detected in 1344 females and 465 males with a thyroid disorder, and also, the prevalence was significantly higher (p &lt, 0.05), compared to the normal thyroid group. Thyroid disorders are a common problem in our population, more prevalent in females than males, with the peak age of above 30 years, and are associated with an increased prevalence of anemia.

Objective: To examine whether the platelet index would be applicable for the diagnosis of community-acquired pneumonia (CAP). Methods: In this study, 64 CAP patients (the case group) and 68 healthy children (the control group) were included from 2017 to 2018. Baseline variables were recorded including total white blood cells, neutrophils, lymphocytes, red blood cells, platelet, mean platelet volume, platelet distribution width, erythrocyte sedimentation rate, and C-reactive protein, and compared between the case group and the control group. The cutoff value, sensitivity, and specificity of neutrophil-to-lymphocyte ratio, platelet, neutrophils, lymphocytes, and platelet larger cell ratio were calculated by receiver-operating characteristic curves. Results: The median platelet count of the case group and the control group were (411.09±67.40) mm3 and (334.48±78.15) mm3, respectively (P=0.000). The median neutrophil count of the case group was higher than that of the control group (P=0.000), while the lymphocyte level of the case group was lower (P=0.000). Differences in other variables including the mean platelet volume, platelet distribution width, C-reactive protein, and erythrocyte sedimentation rate were not statistically significant between the two groups. Conclusions: Due to the different levels of platelet, neutrophil and lymphocyte indices in the case and the control group, these indices can be used simultaneously for the diagnosis of CAP.

Lead induced oxidative stress contributes to increase the productivity of reactive oxygen species (ROS) and to disrupt the antioxidant balance. Gynura procumbens adventitious root (GPAR) methanol extract contains abundant phenolic and flavonoids compounds as antioxidants and can be used as traditional medicinal plants. The objective of this study is to evaluate the protective effect of GPAR against lead acetate toxicity in mice to haematological parameter, histological of hepatic cells, and activities of antioxidant enzymes. The data obtained from five groups of treatment: P1 (control), P2 (Pb acetate-100 mg/L), P3 (GPAR-100 mg/L + Pb acetate-100 mg/L), P4 (GPAR-200 mg/L + Pb acetate-100 mg/L), P5 (GPAR-300 mg/L + Pb acetate-100 mg/L). The results indicated that administration of methanol extract of GPAR can prevent the decreasing of haematological parameter, maintain the percentage of normal hepatic cells, activities of superoxide dismutase (SOD), and catalase (CAT) due to lead acetate treatment. The effective dose of GPAR extract was 300 mg/L. This study provides that methanol extract of G. procumbens adventitious root exerts protective effects against lead acetate toxicity in mice.

Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, which can be acquired by oral contact and may cause severe health problems especially for pregnant (congenital toxoplasmosis) and immunocompromised patients. This study aimed to verify the diagnostic significance of hematological parameters and C-reactive protein (CRP) for toxoplasmosis acute detection. A case-control study was carried out between December 2017 and May 2018, in samples of convenience independent of age and sex. The case group was formed by 25 patients with positive anti-Toxoplasma gondii IgG/IgM antibody and the control group was formed by 21 patients with non-positive anti-Toxoplasma gondii IgG/IgM antibody. The results of the hematological parameters and CRP were analyzed in these patients. The patients with Toxoplasma gondii IgM antibody reagent showed higher lymphocytes counting and lower neutrophils counting than the control group. C-reactive protein levels were not different between the groups case and control. ROC curve analysis highlighted that the cut-off value of > 32.00% for lymphocytes and

El objetivo de este experimento fue evaluar la relación entre diferentes grados de severidad de las miopatías de pechuga, conocidas como pechuga de madera (PM) y estrías blancas (EB), con indicadores de bienestar fisiológicos, productivos y de comportamiento en pollos de engorde. Un total de 570 pollos de engorde Ross 308 fueron criados bajo condiciones comerciales por 13 semanas. Previo al sacrificio, de la semana 7 a la 13, la pechuga de 35 pollos fue analizada al azar, y de acuerdo con su consistencia, fue clasificada en suave, intermedia y firme. Un total de 15 muestras de sangre de los 35 pollos fue obtenida para determinar la relación heterófilolinfocito, proteína C reactiva y el perfil hematológico. Se realizó semanalmente la observación del comportamiento del grupo por observación continua. Se registró el peso vivo, el peso de la pechuga, el peso en canal caliente y de la canal fría. Antes del sacrificio, cada pechuga se clasificó según el grado de severidad de lesiones de EB y PM en normales, leves, moderadas y severas; mientras que las pechugas procesadas se palparon y se clasificaron en suave, intermedia y firme. A cada una se le midió la fuerza y la energía de corte. No se encontró una correlación entre los indicadores fisiológicos y la severidad de las lesiones para ambas miopatías. La severidad de las lesiones aumentó y estuvo correlacionada significativamente (p < 0.01) con la edad, el peso vivo y el peso de la pechuga. La palpación de la pechuga de los pollos vivos estuvo altamente correlacionada con la palpación de la pechuga procesada (p < 0.01). Por tanto, la palpación en vivo puede ser utilizada como un indicador no invasivo de la consistencia y severidad de las lesiones de ambas miopatías. Se requiere evaluar más indicadores fisiológicos, de comportamiento y de dolor en pollos que presenten EB y PM. The aim of the experiment was to evaluate the relationship between different severity degrees of two broiler breast myopathies (wooden breast [WB] and white striping [WS]), and physiological, productive, and behavioral welfare indicators. For this purpose, 570 broilers (Ross 308) were raised for 13 weeks under commercial conditions. From week 7 to week 13, 35 chicken breasts were randomly chosen, and were palpated and classified as soft, intermediate, and firm according to their consistency. From those 35 chickens, 15 sera samples were randomly collected, to obtain heterophil/lymphocyte (H/L) ratio, C­reactive protein (CRP) concentration, and hematological profile. The behaviour of the group was recorded weekly by continuos observation. Live weight, breast weight, hot carcass, and cold carcass weights were recorded. Each chicken breast was classified, as normal, mild, moderate, and severe according to the degree of severity of WB and WS lesions. Processed chicken breasts were palpated and classified as soft, intermediate, and firm. For each one, the shear force and shear energy were measured. Results showed that physiological indicators were not related to the severity of the lesions found in both myopathies. The severity of the lesions was significantly (p < 0.01) correlated with age, live weight, and breast weight. Live chicken breast palpation was highly correlated with processed chicken breast palpation (p < 0.01); therefore, in vivo palpation can be used as a non­invasive indicator of lesion consistency and severity in both myopathies. The evaluation of others physiological, behavioral, and pain indicators in chickens with WS and WB myopathies is required. UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro de Investigación en Nutrición Animal (CINA) UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Zootecnia

This study evaluated how the feeding frequency impacts growth performance, haematological parameters, fatty acid profile and liver histology in meagre (Argyrosomus regius). Fish (15.48 ± 0.11 g) were fed to satiation with a diet formulated according to the nutritional requirements of the species (48% crude protein and 18% crude lipid) for 12 weeks in six different feeding frequencies; 1, 2, 3, 4, 5 times in 24 h (F1, F2, F3, F4, F5) and 2F1 as one day feeding-one day fasted. The final mean weight (FMW) and specific growth rate (SGR) of the F3, F4 and F5 were significantly higher than the F1 and 2F1 groups. Viscerosomatic index (VSI) of the F1 and F3 groups was markedly different from the 2F1. Moreover, packed cell volume (PCV) and haemoglobin (Hb) counts in the F3 and F5 groups were higher than in the 2F1 group. The liver lipid vacuolization was found at more than moderate and also high levels for the groups of F3, F4 and F5 compared to the other groups in histological analysis. The fish in the 2F1 feeding frequency application was negatively impacted by growth performance, feed conversion, condition factor and haematological parameters. Although more feed is consumed in the F4 and F5 groups, the F3 group has a higher economic profit index (EPI). It has been concluded that a higher growth rate and improved health status can be achieved by feeding three times a day (F3) in the practice of mariculture of meagre.

Introduction: Human adaptation to high altitude is due to characteristic adjustments at every physiological level. Differences in lipid profile and cardiovascular risk factors in altitude dwellers have been previously explored. Nevertheless, there are no reports available on genotype-controlled matches among different altitude-adapted indigenous populations.Objective: To explore the possible differences in plasma lipid profile and cardiovascular risk among autochthonous Kiwcha people inhabitants of low and high-altitude locations.Methodology: A cross-sectional analysis of plasmatic lipid profiles and cardiovascular risk factors in lowland Kiwchas from Limoncocha (230 m) and high-altitude Kiwchas from Oyacachi (3,800 m).Results: In the low altitude group, 66% were women (n = 78) and 34% (n = 40) were men, whereas in the high altitude group, 59% (n = 56) were women and 41% (n = 41%) were men. We found the proportion of overweight and obese individuals to be higher among low altitude dwellers (p < 0.05). Red blood cells (RBCs), hemoglobin concentration, and SpO2% were higher among high altitude dwellers and the erythrocyte size was found to be smaller at high altitude. The group located at low altitude also showed lower levels of plasma cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL), but most of these differences are not influenced by gender or elevation.Conclusions: Living at an altitude elicits well-known adaptive physiological changes such as erythrocyte count, hemoglobin concentration, hematocrit level, and serum glucose level. We also report clinical differences in the plasma lipid profile, with higher levels of cholesterol, HDL, and LDL in inhabitants of the Andes Mountain vs. their Amazonian basin peers. Despite this, we did not find significant differences in cardiovascular risk.

This experiment was conducted to investigate the efficacy of tulsi leaves (Ocimum sanctum) extract supplementation in drinking water as a growth promoter in broiler chickens. A total No. of 30 day old Cobb-500 broiler chicks was purchased from local hatchery and after seven days of acclimatization chicks were randomly divided into two groups A and B in equal number. The group A was kept as a control and not treated. The group B was supplemented with tulsi leaves extract with feed and water. Weekly observations were recorded for live body weight gain up to 30 days and hematological tests were performed at 30th day’s age of broiler to evaluate the hematological changes between two groups. The initial body weight of groups A and B on 1st day of this experiment were 42.80±10.31 gm and 42.68±6.90 gm respectively and after 30thdays of experiment final body weight were 1530± 33.07 gm and 1600± 44.80 gm respectively; the net body weight gain from 7thday were 1488±41.12 gm and 1551±39.29 gm respectively and economics of production was analyzed and found that net profit per broiler was Tk. 24.51 and Tk. 32.50 respectively. The treatment group B was reported statistically significant (at 1% level) increased 16.43% for live body weight than the control group A. The hematological parameters TEC, ESR and PCV value of treatment group showed significant difference, while Hemoglobin (Hb) estimation did not show significant difference from control group. Therefore, the findings of the study results suggest that supplemented with tulsi leaves extract with feed and water can improve the growth performance of broiler.

Hematological features are one of the best-known aspects of high-altitude adaptation in Tibetans. However, it is still unclear whether the intestinal microbiota is associated with the hematology profile. In this study, routine blood tests and 16S rRNA gene sequencing were used to investigate the differences in the intestinal microbiota and hematological parameters of native Tibetan herders and Han immigrants sampled at 3,900 m. The blood test results suggested that the platelet counts (PLTs) were significantly higher in native Tibetans than the Han immigrants. The feces of the native Tibetans had significantly greater microbial diversity (more different species: Simpson’s and Shannon’s indices) than that of the Han immigrants. The native Tibetans also had a different fecal microbial community structure than the Han immigrants. A Bray–Curtis distance-based redundancy analysis and envfit function test showed that body mass index (BMI) and PLT were significant explanatory variables that correlated with the fecal microbial community structure in native Tibetans. Spearman’s correlation analysis showed that Megamonas correlated positively with BMI, whereas Bifidobacterium correlated negatively with BMI. Alistipes and Parabacteroides correlated positively with the PLT. Succinivibrio correlated positively with SpO2. Intestinibacter correlated negatively with the red blood cell count, hemoglobin, and hematocrit (HCT). Romboutsia correlated negatively with HCT, whereas Phascolarctobacterium correlated positively with HCT. A functional analysis showed that the functional capacity of the gut microbial community in the native Tibetans was significantly related to carbohydrate metabolism. These findings suggest that the hematological profile is associated with the fecal microbial community, which may influence the high-altitude adaptation/acclimatization of Tibetans.

Background and Aim: Theileriosis is a parasitic disease caused by the hemoprotozoan Theileria spp. The main transmission route of this disease is through tick vector bite. Theileriosis causes economic losses in livestock such as Bali cattle. This study aims to analyze the prevalence of theileriosis and its correlation with the hematological profile of Bali cattle from the subdistrict of Muara Bulian, Batang Hari, Jambi, Indonesia. Materials and Methods: Ninety-four blood samples were collected through jugular vein of Bali cattle. The presence of Theileria spp. was determined using blood smear. Routine blood tests and double-staining immunohistochemistry against CD4+ and CD8+ lymphocytes were conducted on all blood samples. Results: A total of 34.04% of the samples were infected by Theileria spp. Theileriosis affected only hemoglobin level (p0.05). However, it also decreased CD4+ and CD4+/CD8+ ratio (p

This study aims to investigate the correlation between the body condition score (BCS) and hematological profiles of stray dogs in Maha Sarakham province. The primary assessment of dog’s health was determined for screening their health problems such as the cadaverous condition or diseases. The BSC of 65 stray dogs, which were divided into 34 male dogs (52.31 %) and 31 female dogs (47.69%), were assessed and their blood were collected for determining the hematology profiles by an automated hematological analyzer. The results were analyzed and the correlation between BCS and hematology profiles was investigated. The results showed that the average of BCS in tested canine was 2.66 ± 0.59 (lower than 3.5) which is defined as cadaverous condition. Moreover, all of studied canines exhibited the thrombocytopenia condition. This study showed that sex and BCS did not significantly affect the hematology profiles. Furthermore, there were no significant relationships between the hematology profiles and BCS.

Hertika AMS, Supriatna, Darmawan A, Nugroho BA, Handoko AD, Qurniawatri AY, Prasetyawati RA. 2021. The hematological profile of Badher fish (Barbonymus altus) to evaluate water quality in the Badher Bank Conservation Area, Tawangrejo Village, Blitar District, East Java, Indonesia. Biodiversitas 22: 2532-2541. Badher fish (Barbonymus altus) is endemic to the Brantas River. After years presumably extinct, the Badher fish was found again in 2010 in the Brantas River, Tawangrejo Village, Binangun Sub-district, Blitar District, East Java, Indonesia. A conservation area, called a Badher Bank, was established in the location. However, human activities, such as ecotourism and agricultural waste runoff, have changed water quality and increased water pollution in the Badher Bank Conservation Area. This research objective was to observe the hematological profile of badher fish and a set of water quality parameter measurements as an environmental factor that affected fish life. Data were obtained from four research stations with three replications every two weeks from August to October 2020 at the Brantas River streamline inside the Badher Bank Conservation Area. The Canonical Correspondence Analysis (CCA) method was applied to determine the correlation between water quality and hematological profile. According to the data analysis, the water quality at Stations 1-3 in the research location was normal, and Station 4 was polluted. The analysis also showed that the low dissolved oxygen and high ammonia affected the hematological conditions of Badher fish. All water quality parameters affected the condition of leucocytes, erythrocytes, and hemoglobin in moderate concentrations. Besides that, micronuclei and hematocrit conditions were strongly influenced by ammonia, temperature, pH, and current velocity in high concentrations, while Dissolved Oxygen (DO) and Total Suspended Solid (TSS) affected low concentrations.

This study aimed to determine the impact of immersing pikeperch (body weight approximately 94 g) in an aqueous solution of sodium chloride on gasometric, hematological, and biochemical indicators. The impact of the sodium chloride concentration (NaCl 10, 15, and 20 g L−1), immersion time (30 or 60 min) and time of blood sampling (0 h – blood drawn immediately following fish immersion; 24 h – blood drawn one full day after manipulation) was analyzed. The immersion protocol did not bring significant stress for tested fish as none of the tested parameters was different when compared to results obtained in a blank control group. The immersion times tested impacted the gasometric indicators and blood acid-base balance (0 h). The levels of most of the whole blood and plasma biochemical indicators analyzed (Na+, Cl-, glucose, lactate, total protein, globulin) differed significantly in fish exposed to 15 g L−1 and 20 g L−1 of NaCl from those in the blank control group. These changes were short-term and after 24 h the values of these indicators did not differ from those confirmed in the control group. Significant increases in the levels of hemoglobin, hematocrit, and red blood cells were confirmed in groups 10 g NaCl L−1/30 min/0 h and 20 g NaCl L−1/30 min/0 h and these changes persisted 24 h following immersion. Significant increases in the number of white blood cells (0 h) were confirmed in all groups, and this persisted for 24 h following immersion. The current study revealed transient, dose- and time-dependent stress reactions in pikeperch subjected to abrupt changes in water salinity and indicated that this species has significant recovery abilities in response to salinity shifts of short duration.

Graphical abstract, Highlights • The manuscript is interest with the brilliant insecticide Thiamethoxam health risk. • Hematological, biochemical and genetic studies were carried to investigate the Thiamethoxam induced alterations. • Moringa oleifera role against thiamethoxam alterations was studied., Thiamethoxam (TMX) exerts pronounced insecticidal effects against a wide variety of economically imperative pests. However, the administration of TMX in experimental animals induced notable adverse effects on the function of various organs. The purpose of this study was to assess TMX induced hematological, biochemical, and genetic alterations and the potential ameliorative effects on them of Moringa oleifera leaf extract (MLE) in male mice Animals were orally administered TMX (≈1/10 LD50) daily either alone or with MLE (200 mg/kg b.w.) for 28 successive days. Blood was collected to evaluate the hematological profile and serum levels of Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, creatinine, uric acid, and urea. Liver and kidney cells were used to assess the Malondialdehyde (MDA) content and antioxidant enzymes. DNA integrity was estimated also in the liver and kidney using comet and colorimetric diphenylamine assays. Results revealed that TMX exhibited significant changes in the hematological profile and liver and kidney functions. Besides, TMX significantly raised the MDA content and DNA damage in both two of these organs. In contrast, TMX reduced the antioxidant activities in the cells of both liver and kidney. Meanwhile, Moringa extract combined with TMX significantly attenuated the deleterious findings of TMX. Specifically, it improved the TMX-induced hematological changes, liver and kidney function alterations, oxidative stress, and DNA damage rate. It can be concluded that TMX had adverse effects on different cells of male mice, but MLE successfully ameliorated TMX’s hematological and hepatorenal toxicity.

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ, This research aimed to investigate the influence of different age of Podolian grey steppe and Holstein Friesian cows, as well as the effects of different breeds on their hematological and biochemical blood profile. A total of 128 adult cows aged from 3 to 12 years were used in the study. Gained results show statistically significant differences (P < 0.05) regarding all hematological parameters. The concentration of NE was recorded in the optimal range for Podolian grey steppe cows, while for Holstein Friesian breed was elevated.Values for LY was in the optimal range, while the values for MO, EO, and BA were slightly higher for both breeds of cow in all groups, with recorded significant differences (P < 0.05).Count of RBC in the Podolian grey steppe breed ranged between 5.83 and 11.21×1012/L, with significant influence (P < 0.05) of cows age in obtained values. In contrast, Holstein Friesian cows breed recorded RBC values lover then optimal, without any significant difference (P > 0.05) related to the age of cows. Total protein has ranged between 7.10 and 8.50 g/dl (Podolian grey steppe), and 8.13 to 8.48 g/dl (Holstein Friesian), without recorded statistically significant differences (P > 0.05) between the age, breed as well as the interaction of age and breed. Asimilar tendency can be seen regarding the otherbiochemical values (P>0.05), respectively.Gain results have mainly shown a significant influence of breed on the parameters above, while the significant influence of age of cows was not present as well the interaction between age and breed.

Background Blastocystis spp. are considered pathogenic or commensal organisms, although the majority of researchers suggest that these are neglected pathogens. The main aim of this study was to determine the prevalence and subtype distribution of Blastocystis spp. in pre- and perimenopausal women, with respect to socio-demographic (age and place of residence), and epidemiological factors, as well as drinking tap water, contact with domestic animals, traveling abroad, health status, and presence of gastrointestinal symptoms. Additionally, the objective was to compare hematological and biochemical parameters of Blastocystis spp. infected and uninfected women. Methods The study included 425 women aged 45–60. Their stool samples were examined microscopically and analyzed by a conventional polymerase chain reaction (cPCR). Results Blastocystis spp. were detected in 6.1% of pre- and perimenopausal women. Molecular analysis of the stool samples identified seven Blastocystis subtypes (ST1–ST4, ST6, ST7, and ST9). Blastocystis subtypes 2 and 3 were the most prevalent. The presence of Blastocystis spp. was not significantly related to socio-demographic and epidemiological factors. There were also no significant associations between Blastocystis spp. and blood parameters, or gastrointestinal symptoms. Conclusion This study complements the limited available data on the prevalence of Blastocystis spp. in pre- and perimenopausal women. It is also the first report showing the presence of Blastocystis subtype 9 in Poland.

International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 12, 12, 12A12E: 1-11

Dengue virus, the etiological agent of dengue fever (DF) occurs in four genetically distinct serotypes (DENV1-4), being transmitted by female Aedes mosquitoes. DF incidence is increasing in Brazil, following vector dispersal, proliferation and DENV serotypes introduction, co-circulation and substitution. Medium- and small-sized cities in Sao Paulo State, such as Marilia (Midwest region), have been affected by huge epidemics. To understand the evolution of DENV epidemics in medium-sized cities, in this study a historical data on DENV incidence (2000-2015) in Marilia, was evaluated. Previous studies disclosed regional and specific DF outcomes associated with 2007 outbreak in that city, when co-circulating DENV1 and DENV3 presented different hematological profiles. In this study, characteristics of 2007 DENV epidemics were compared to the epidemiological, hematological and demographic outlines of the major outbreak of DENV1 in Marilia in 2015. DENV1 genetic diversity was assessed through capsid and pre-membrane junction encoding gene (CprM) sequencing. The results revealed circulation of DENV1 serotype from 2007 to 2015, with epidemics occurring every three-years until 2013 and then, increasing yearly. There were significant differences in hematological profiles of DENV1 patients between 2015 and 2007. CprM showed DENV1 genetic variability in 2015, contrasting with the unique sequence pattern in 2007. These results reinforce the regional and temporal characteristics of DENV epidemics that need local public health research to improve care for people and to limit the spread of new serotypes/genotypes to uninfected areas.

The objective of this study was to evaluate the effects of dietary supplementation of a polyherbal additive (PA) containing hydrolyzable tannins, flavonoids, and essential oils on productive performance, dietary energetics, carcass and meat characteristics, and blood metabolites of lambs in their finishing phase. Twenty-eight Pelibuey × Katahdin lambs (20.52 ± 0.88 kg body weight (BW)) were housed in individual pens and assigned to four treatments (n = 7) with different doses of PA: 0 (CON), 1 (PA1), 2 (PA2), and 3 (PA3) g of PA kg−1 of DM for 56 days. Compared to the CON, lambs in PA1 treatment had higher average daily gain (p = 0.03), higher dietary energy utilization (p = 0.01), greater backfat thickness (p = 0.02), greater Longissimus dorsi muscle area (p = 0.01), and better feed conversion ratio (p = 0.02). PA supplementation did not affect (p > 0.05) dry matter intake, carcass yield, biometric measures, and meat chemical composition. All hematological and most of the blood biochemical parameters were similar in lambs of all treatments (p > 0.05). However, compared to the CON, lambs assigned to the PA3 treatment had lower serum urea concentration (p = 0.05) and higher serum albumin concentration (p = 0.03). In conclusion, low doses of PA could be used as a growth promoter in finishing lambs without affecting dry matter intake, carcass yield, meat chemical composition, and health status of the lambs. However, more in vivo research is needed to better understand the impact of bioactive compounds from PA used on productivity, metabolism, and health status of finishing lambs.

Funding: This research was supported by project BE4AQUAHEALTH: RASTREIO NACIONAL DE PATOLOGIAS DE PEIXES DE AQUACULTURA: UMA APOSTA NA PREVENÇÃO (MAR-02.05.01-FEAMP-0013), funded by Mar2020 Operational Programme and the European Union through FEDER and by Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2020 granted to MARE and within the scope of UIDB/04423/2020 and UIDP/04423/2020 granted to CIIMAR. BC was supported by FCT, Portugal (2020.00290.CEECIND). Currently, aquaculture production of rainbow trout (Oncorhynchus mykiss) is a multibillion dollar industry; nevertheless, the development of this sector has not been exempt from pitfalls related to the recurrent presence of pathogens of bacterial origin. This is the case of Yersinia ruckeri, the etiologic agent of the infectious pathology known as Enteric Red Mouth Disease (ERM), causing serious economic losses that can be as high as 30–70% of production. Although several studies have been performed regarding pathogen features and virulence factors, more information is needed about the host defense mechanism activation after infection. Given this perspective, this study aimed to evaluate rainbow trout’s short-term innate immune response against infection with Y. ruckeri. A series of factors linked to the innate immune response were evaluated, including determination of hematological parameters, oxidative stress biomarkers, and analysis of the expression of immunerelated genes. Results showed a significant decrease in several hematological parameters (white blood cell count, hematocrit, neutrophils, monocytes, lymphocytes, and thrombocytes) and oxidative stress indicators (SOD) between the control and infected groups. In addition, there were significant differences in the level of gene expression between infected individuals and the control group. Most of these genes (il-1b, il-8, il-10, tnf-a1, tnf-a2, socs3, mmp-9, cath, hsp-70, saa, fer, pcb) were upregulated within the first 24 h following infection. Results from this study showed more insights into the short-term immune response of rainbow trout to infection with Y. ruckeri, which may be useful for the establishment of biomarkers that may be used for the early detection of ERM. info:eu-repo/semantics/publishedVersion

Цель исследований заключалась в установлении компенсаторно приспособительных механизмов овец карачаевской породы, содержавшихся в разных условиях, а также перегоняемых в летний период на пастбища с разной высотой над уровнем моря. Исследования выполнялись на баранчиках карачаевской породы, принадлежащих племенному репродуктору ООО «Дарган» Кабардино -Балкарской Республики, содержащихся круглый год на пастбище – на высоте 2200 м над уровнем моря (I группа), в предгорье с использованием в летний период присельских пастбищ ( II группа), при отгонно-горном содержании в пастбищный период на высоте 1200 ( III группа) и 2200 м (IV группа) над уровнем моря (н.у.м.). Изу чение показателей гематологического и клинического статуса, резистентности проводили при формировании групп животных – в возрасте 4 месяца, 4,5 месяца – на 5-й день пребывания на пастбище, 6 месяцев – на 50-й день и 9 месяцев – после возвращения с пастбищ (окончание пастбищного периода) по общепринятым методам. Установлено, что у баранчиков I, II и III групп клинические показатели находились на более низком уровне, чем у сверстников предгорного содержания, особенно при высокой положительной температуре воздуха. Независимо от возраста и места содержания наибольшее содержание гемоглобина в крови было свойственно баранчикам круглогодового пастбищного содержания, они же отличались более интенсивным фагоцитозом, что является приспособительной реакцией к горной ги поксии. Перевод поголовья овец на пастбища различной высоты также сопровождалось увеличением концентрации гемоглобина и фагоцитарной активности нейтрофилов крови. Выращивание молодняка карачаевской породы в условиях круглогодового содержания в горах и по т ехнологии отгонно-горного содержания в отличие от содержания в предгорье, особенно в летний период, является менее напряженным для органов и тканей организма, что обеспечивает наилучшее приспособление к высотной поясности территории Северного Кавказа., The purpose of the research was to establish the compensatory-adaptive mechanisms of Kara-chai sheep kept in different conditions, and also distilled to pastures in summer with different heights above sea level. The studies were carried out on sheep of Karachai breed belonging to the pedigree reproducer Dargan LLC of the Kabardino-Balkarian Republic kept in the pasture year-round at the altitude of 2200 m above sea level (group I), in the foothills with the use of summer pastures in summer (group II) and with distant-mountain maintenance in the grazing pe-riod at the altitude of 1200 (group III) and 2200 m (group IV) above sea level (a.s.l.). It was car-ried out the investigation of hematological, clinical status indicators and resistance during the formation of animals’ groups of the age of 4 months, at the age of 4.5 months on the 5th day of stay in the pasture, in 6 months is on the 50th day and 9 months after returning from pasture (at the end of grazing period) according to generally accepted methods. It was established that in the rams of groups I, II and III, the clinical indicators were at a lower level than in peers of the foot-hill keeping, especially during high positive air temperature. Regardless of age and place of keeping, the highest hemoglobin content in the blood was characteristic in rams of year-round pasture keeping, they are also differed in more intense phagocytosis, which is an adaptive reac-tion to mountain hypoxia. The transfer of sheep to pastures of various heights was also accom-panied by an increase in the concentration of hemoglobin and phagocytic activity of blood neu-trophils. The growing of young Karachai breed under conditions of year-round keeping in the mountains and according to the technology of distant-mountain maintenance is less stressful for the organs and tissues of organism, in contrast to the content in foothills, especially in summer, which provides the best adaptation to the altitude zonality of the North Caucasian territories.

Tezel, Rifat/0000-0002-0870-7049; Yigit, Murat/0000-0001-8086-9125; fazio, francesco/0000-0003-3198-2580; KESBIC, Osman Sabri/0000-0002-1576-1836; YALGIN, Ferhat/0000-0002-0027-5114; Yilmaz, Sevdan/0000-0002-4809-5809 WOS: 000508416200003 This study aimed to carry out a comparative evaluation of the hematological profile (erythrocyte count, hemoglobin concentration, hematocrit, and Wintrobe index parameters such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), in addition to certain biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, scrum total protein, albumin, glucose, total cholesterol, and triglycerides), in the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810). Accordingly, two groups of fish were used in this study: 30 flathead grey mullets, M. cephalus, caught in Lake Faro, Italy, and 30 golden grey mullets, C. auratus, caught in the estuarine channel system of Keycegiz-Dalyan, Turkey. Statistical evaluations (unpaired t-test) revealed that there were no significant differences in weight or total length values between the two mullet groups. However, the differences between all the evaluated blood parameters (except alanine aminotransferase) between the Italian and the Turkish mullet were significant (P < 0.0001). The present study demonstrated that there were significant hematological and biochemical differences between the Italian (M. cephalus) and the Turkish (C. auratus) mullet. Our findings also contribute to expanding the knowledge on the hematology and biochemistry of two different species of mullet originating from two different habitats. Since the evaluation of blood parameters represents an essential tool in examining the effects of environmental conditions on fish physiology and, consequently, on fish health status, further research in this field is encouraged and would be very useful.

Background and aim: The assessment of hematological profile requires to identify possible sources of biological variability, including exercise-related variations. This study was hence aimed to evaluate hematological profile variations in amateur and professional athletes, and establish their possible dependence on cumulative training volume. Materials and Methods: The study population consisted of 59 sedentary male subjects, 78 amateur and 80 professional male cyclists, in whom a large number of hematological variables were measured at rest. Results: Red blood cell (RBC) count and hemoglobin were decreased in the two athlete cohorts compared to sedentary subjects, but did not differ between amateur and professional cyclists. Hematocrit was gradually and significantly decreased in parallel with cumulative training volume. Amateur cyclists displayed higher mean corpuscular volume (MCV) and lower mean corpuscular hemoglobin concentration (MCHC) values than sedentary subjects and professional cyclists, whilst mean corpuscular hemoglobin (MCH) was higher in professional cyclists. The reticulocyte count and soluble transferrin receptor (sTFR) values were similar across all groups. Serum ferritin was higher in professional cyclists than in the other two groups, whilst transferrin gradually decreased from sedentary group to the two cohorts of amateur and professional cyclists. In univariate analysis, cumulative training volume was inversely associated with age, body mass index (BMI), RBC count, hematocrit, hemoglobin and transferrin, whilst a positive association was found with ferritin. In multivariate analysis, BMI, RBC count and ferritin remained significantly associated with training volume. Conclusions: These results show that the volume of endurance training may affect some hematological variables. (www.actabiomedica.it)

Background and Aim: Foot-and-mouth disease (FMD) is an acute viral infection affecting cloven-hoofed animals causing vesicular erosions in the oral cavity and interdigital space. The present study was undertaken to ascertain the time-dependent changes in clinical, hematological, and biochemical profiles in different breeds of cattle following experimental infection. Materials and Methods: The animals were inoculated with 1.0×104 50% bovine tongue infectious dose (BTID50) by intradermolingual route. Clinical signs were observed, and blood/serum samples were collected at different time intervals. Results: The white blood cell count declined sharply on days 7-13 and recovered on day 14 post-FMD infection. Biochemical analysis of serum markers for vital organ profile revealed no marked damage. However, a significant increase in blood urea nitrogen (BUN) value indicated pre-renal azotemia. Transient hyperthyroidism was indicated by the rise in T3 and T4 that can be correlated with a decrease in triglyceride and total cholesterol levels. In the cardiac damage assessment study, a distinct breed difference was observed wherein Malnad Gidda calves showed no cardiac damage. Conclusion: Except thyroid profile, BUN, and creatine kinase-myocardial band, all other serum biochemical parameters showed no significant abnormalities, whereas lymphopenia is the only hematological change and it is suggested that effective ameliorative measures should be targeted mainly on the feed/water intake, thyroid gland, and the level of lymphocytes.

Background In pregnancy, hematological changes occur in order to meet the demands of the developing fetus and placenta, with major alterations in blood volume. Abnormal hematological profile affects pregnancy and its outcome. This study aimed to assess hematological profiles of pregnant women at a tertiary care teaching hospital. Method This cross sectional study was conducted among 284 consecutive pregnant women at St. Paul’s Hospital Millennium Medical College. Socio-demographic characteristics were collected using pre-tested structured questionnaire. About 4 ml of venous blood was collected from each participant for hematological parameters analysis using Cell-Dyn1800 (Abbott Laboratories Diagnostics Division, USA) and peripheral blood film review. Result There were differences in mean hematological parameters between trimesters: specifically differences in mean values of WBC (1stand 3rd), Hb(1stand2nd and 1st& 3rd), HCT (1stand2nd), RDW (1stand2nd and 1stand3rd), neutrophil and lymphocyte (1stand 2nd and 1stand3rd, for both) were statistically significant (p

The aim of this work was to determine the impact on European perch, Perca fluviatilis L. (mean body weight – 78.33 g) of the intraperitoneal implantation of telemetry transmitters using different suturing methods. In the first experiment silk sutures were used (experiment I – group ST), while in the second tissue adhesive was used (experiment II – group GT). Following the procedure, the fish were kept for 42 days in a recirculating system. Differences in growth and condition parameters were only noted in the first week of the experiment. Specimens from group GT had lower values for DGR (daily growth rate) and SGR (specific growth rate), but a higher value for FCR (feed conversion ratio) values. For the hematological parameters, lower values of MCV (mean corpuscular volume) and PLT (blood platelets) were noted in group GT, while for the biochemical parameters, lowered ALP (alkaline phosphatase) activity and Mg (magnesium) concentrations were noted in group ST. In group ST, 33.3% of the specimens loss their tags, while in group GT 77.8% did so. Differences in incision healing were only noted in the second week, when specimens in group ST were observed to have fully closed incisions, while in group GT 50% of the incisions were open. Despite the high percentage of implantation incision healing in both groups, because of the high values of tag loss rate, neither method can be recommended for perch. It might be more effective to use tag with external antennae in this species. The method use for closing implantation incisions also must be improved to eliminate tag shedding.

The aim of this study was to evaluate the effect of diet containing mung bean sprout waste (MBSW) and feeding times on the hematological profiles and metabolite status of garut lambs. Experimental design used in this study was a factorial randomized block design with two factors of diet and two feeding times. Two factors of diets were 60% concentrate 1+40% natural grass and 60% concentrate 2+40% MBSW. Feeding times were in the morning (6:00-7:00 am) or the evening (5:00-6:00 pm). The animals were reared in individual cages and fed with 5% DM/kg body weight/day. The hematological profiles of the goat were examined and analyzed with Anova. The results showed the treatment of rations significant effect on blood glucose, whereas feeding time very significant to glucose, erythrocytes and hemoglobin, and the interaction of both treatments significantly affect hemoglobin, hematocrit, and glucose level. Meanwhile,leukocytes, neutrophils, lymphocytes, neutrophils/lymphocyte ratio (N/L), urea-N, and blood cholesterol were not affected by the treatments and their interactions. But overall the number of erythrocytes 8.72-12.78x106/mm3, hemoglobin 7.6-10.02 g/dL, leukocytes 8.94-12.27x103/mm3, neutrophils 33.00-52.20%, lymphocytes 38.80-52.40%, N/L 0.78-1.66, glucose 41.94-54.24 mg/dL, urea-N 29.91-35.87 mg/dL, and blood cholesterol 24.57-30.28 mg/dL. These results suggest that diets containing 40% MBSW together with a morning or an evening feeding time did not cause disturbances in haematological profile and blood metabolite status of garut lamb. ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efek ransum mengandung hijauan limbah tauge dan waktu pemberian yang berbeda terhadap profil hematologi dan status metabolit darah domba garut. Materi penelitian berupa 20 ekor domba garut berumur 6-7 bulan (bobot badan 15,42 ± 2,42 kg) dipelihara selama tiga bulan dan dikandangkan secara individu serta diberi pakan sebanyak 5% bahan kering/kg berat badan/hari. Penelitian ini menggunakan rancangan acak kelompok pola faktorial dengan dua perlakuan yaitu jenis ransum dan waktu pemberian pakan. Dua jenis ransum yang diformulasikan dengan basis bahan kering (BK) adalah Ransum 1 (60% konsentrat 1 + 40% rumput lapang) dan Ransum 2 (60% konsentrat 2 + 40% limbah tauge). Waktu pemberian pakan terdiri dari pemberian pakan pada pagi hari (06.00-07.00) dan sore hari (17.00-18.00). Pengambilan sampel darah dilakukan pada akhir bulan kedua setelah masa pemeliharaan. Data yang diperoleh dianalisis dengan sidik ragam. Perlakuan jenis ransum berpengaruh nyata terhadap kadar glukosa darah. Waktu pemberian pakan berpengaruh nyata dan sangat nyata terhadap kadar glukosa, eritrosit dan hemoglobin. Interaksi kedua perlakuan tersebut berpengaruh nyata terhadap hemoglobin, hematokrit, dan glukosa darah. Leukosit, neutrofil, limfosit, rasio neutrofil/limfosit (N/L), urea-N, dan kolesterol darah tidak dipengaruhi oleh kedua perlakuan dan interaksinya. Secara keseluruhan jumlah eritrosit 8,72-12,78 x 106/mL, hemoglobin 7,6-10,02 g/dL, leukosit 8,94-12,27 x 103/mL, neutrofil 33,00-52,20%, limfosit 38,80-52,40%, N/L 0,78-1,66, glukosa 41,94-54,24 mg/dL, urea-N 29,91-35,87 mg/dL, dan kolesterol darah 24,57-30,28 mg/dL. Simpulan penelitian adalah ransum yang mengandung 40% limbah tauge dengan waktu pemberian pakan pagi atau sore hari tidak menyebabkan gangguan pada profil hematologis dan status metabolit darah domba garut.

Aim: The aim of this study was to evaluate the effect of feeding Acremonium charticola-fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens. Materials and Methods: A total of 240 male Lohman day-old-chicks were randomly allotted to one of the four experimental diets including control diet, control diet + antimicrobials (neomycin; 300 mg/kg diet), diet containing AC-FCP (16 g/100 g diet) or diet containing AC-FCP + antimicrobials. At day 28, the birds from each pen were blood sampled, sacrificed and immediately the internal organs were removed and weighed. Digesta were obtained from the ileum and cecum. Results: Birds fed AC-FCP had lower (p

Submitted by WILLIAM DOS SANTOS XAVIER (willxaviier@gmail.com) on 2019-06-19T16:58:04Z No. of bitstreams: 1 Dissertação (William dos Santos Xavier).pdf: 826486 bytes, checksum: a3e434923b3f75486fc5dbcfc8f14016 (MD5) Approved for entry into archive by Ana Lucia de Grava Kempinas (algkempinas@fca.unesp.br) on 2019-06-19T18:37:42Z (GMT) No. of bitstreams: 1 xavier_ws_me_botfmvz_par.pdf: 264471 bytes, checksum: ee08701aa5e1dd19d418a9365006fe3c (MD5) Made available in DSpace on 2019-06-19T18:37:42Z (GMT). No. of bitstreams: 1 xavier_ws_me_botfmvz_par.pdf: 264471 bytes, checksum: ee08701aa5e1dd19d418a9365006fe3c (MD5) Previous issue date: 2019-06-07 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O objetivo do presente estudo foi avaliar os efeitos do concentrado de melão rico em SOD (superóxido dismutase) sobre o desempenho produtivo, perfil hematológico e atividade das enzimas antioxidantes da tilápia-do-Nilo submetidas a desafio por estresse térmico e hipóxia. Um grupo de 462 tilápias revertidas sexualmente (8,87 g ± 0,12) foi distribuído aleatoriamente em 42 aquários de 250 L (11 peixes/aquário) e alimentado com sete dietas práticas contendo níveis de concentrado de melão 0; 0,1; 0,2; 0,3; 0,4; 0,5 e 1,0% por 60 dias. As dietas foram formuladas para conter 29% de proteína digestível e 18 MJ de energia digestível kg-1. Após 60 dias de alimentação, determinou-se o desempenho produtivo, parâmetros hematológicos e atividade das enzimas do sistema antioxidante. Em seguida, os peixes foram submetidos a desafio térmico e hipóxia (34°C/1,87 mg/L-1 de oxigênio dissolvido) por dois dias e os mesmos parâmetros hematológicos e atividade de enzimas do sistema antioxidantes foram determinados após esse período. No presente estudo, o concentrado de melão foi capaz de manter a eritropoiese e a capacidade antioxidante sob estresse térmico e hipóxia, porém não houve efeito no desempenho produtivo. A concentração de malonaldeído presente no filé de peixes alimentados com dietas suplementadas com concentrado de melão diminuiu 1,5 vezes após o estresse. Os resultados do presente estudo sugerem o nível de suplementação entre 0,4 e 0,5% de concentrado de melão capaz de manter a saúde dos animais após desafio. This study evaluated the effects of SOD-rich melon pulp concentrate (SOD-rich MPC) on growth performance, hematological profile and antioxidant enzyme activity of Nile tilapia subjected heat/dissolved oxygen-induced stress (HDOIS). A group of 462 male Nile tilapia (8.87 g ± 0.12) was randomly distributed in 42 250-L aquaria (11 fish/tank) and fed seven practical diets with graded levels of SOD-rich MPC at 0, 0.1, 0.2, 0.3, 0.4, 0.5 and 1.0% for 60 days. The diets were formulated to contain 30% crude protein and 18 MJ kg-1 crude energy. After the feeding period, growth performance was evaluated and six fish per treatment were sampled for hematological profile and antioxidant enzyme activity. Then, fish were subjected to HDOIS (34°C/1.87 mg/L-1 dissolved oxygen) for two days and the same hematological profile and antioxidant enzyme activity were determined. In the present study, SOD-rich MPC was able to keep erythropoiesis and antioxidant capacity under HDOIS, but did not affect growth performance. The concentration of MDA in fish fed diets supplemented with SOD-rich MPC decreased by 1.5 fold after HDOIS. The results of the present study suggest that 0.5% SOD-rich MPC dietary supplementation was appropriate to maintain Nile tilapia health and improve their antioxidant capacity under HDOIS. 2017/13217-9

Background: Studies comparing serum bilirubin trend and hematological profile of neonates with hemolytic and nonhemolytic etiology are limited. Apart from high bilirubin load, they are at increased risk of developing anemia in early days of life. Methods: A prospective observational study was conducted in neonatal unit on late preterm and term neonates (>35 weeks) with unconjugated hyperbilirubinemia (UH) to compare serum bilirubin trend (initial and peak), hematological profile (hemoglobin [Hb] and hematocrit [PCV]) (initial and at 3 months), between hemolytic and non-hemolytic cases. Result: Among 240 enrolled neonates, initial serum bilirubin level was higher in haemolytic group than non-hemolytic (mg/dl) (16.9 ± 3.2 vs. 14.6 ± 4.1; p

The objective of this study was to determine the effects of the supplementation of a polyherbal mixture (HM) on the productive performance, carcass characteristics, meat quality, and the profile of blood metabolites of lambs fed a high-concentrate diet. Thirty-six male Pelibuey lambs (25.21 ± 0.96 kg BW) were housed in individual pens during a 56-day feeding period and were randomly assigned to four treatments: (1) Control (CON): Basal diet without HM, (2) HM1: CON + 1 g of HM kg−1 dry matter (DM), (3) HM2: CON + 2 g of HM kg−1 DM, and (4) HM3: CON + 3 g of HM kg−1 DM. Data were analyzed using the GLM (General Linear Model) procedure of statistical analysis system (SAS), and linear and quadratic effects were tested to evaluate the effects of the HM level. A quadratic increase was observed in the dry matter intake and in daily weight gain (p &lt, 0.05) of lambs fed with HM2 and HM1, respectively. However, final body weight, body condition, carcass characteristics, and meat quality were similar among treatments (p &gt, 0.05). It was observed a linear increase (p &lt, 0.05) in the mean corpuscular hemoglobin concentration. Lymphocytes in blood from lambs supplemented with the HM1 diet increased and segmented neutrophils decreased compared to lambs receiving the CON treatment (p &lt, 0.05). The concentration of uric acid in the blood had a linear increase (p &lt, 0.05) and the serum creatinine level decreased (p &lt, 0.05) as the HM dietary dose increased. In conclusion, dietary inclusion of 2 and 1 g of HM kg−1 of DM improves feed consumption and daily weight gain, respectively, without affecting carcass characteristics, meat quality, and health status on finishing lambs.

Made available in DSpace on 2020-12-12T02:45:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-15 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study evaluated the effects of SOD-rich melon pulp concentrate (MPC) premix on growth performance, hematological profile and the antioxidant capacity of Nile tilapia (Oreochromis niloticus) subjected to heat/dissolved oxygen-induced stress (HDOIS). A group of 462 male Nile tilapia (8.87 ± 0.12 g) was randomly distributed in 42,250-L tank (11 fish/tank) and fed seven practical diets supplemented with graded levels of MPC (0.0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and 1.0% MPC premix of diet; PM Melofeed 2.5, SOD activity min. 65,000 IU kg−1, Lallemand SAS, Blagnac, France) with six replicate tank/diet. The diets were formulated to contain 30% crude protein and 18 MJ kg−1 crude energy. After 60 days of feeding, growth performance was evaluated and six fish per treatment were sampled for hematological profile and analysis of the hepatic activity of key antioxidant enzymes. Then, remaining fish were subjected to HDOIS (34 °C/1.87 mg L−1 dissolved oxygen) for 2 days and the same hematological profile and antioxidant enzyme activities were determined. MPC supplementation was associated with the recruitment of hepatic antioxidant defense in the form of increased SOD activity prior and after HDOIS as well as of GR activity before HDOIS at 0.4% MPC and above. These levels of MPC supplementation were weakly associated with reduced level of muscle lipid oxidation. This trial indicates that MPC supplementation at or above 0.4% contributes to a better maintenance of antioxidant defense in Nile tilapia when exposed to HDOIS environmental stress. This is expected to yield biological benefits in the longer-term when Nile tilapia are chronically or repetitively exposed to similar environmental stressors as may be the case under intensive aquaculture conditions. Department of Breeding and Animal Nutrition UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho” Lallemand SAS, 19 rue des Briquetiers Department of Production and Plant Breeding UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho” Department of Breeding and Animal Nutrition UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho” Department of Production and Plant Breeding UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho” FAPESP: #2017/13217-9

El dengue constituye una de las enfermedades transmitidas por mosquitos más importante a nivel mundial. La enfermedad puede cursar con un cuadro asintomático, presentarse con un amplio rango de manifestaciones clínicas inespecíficas o cierto porcentaje puede derivar en casos graves. Este estudio observacional descriptivo de corte transverso tuvo como objetivo determinar las características clínicas, parámetros hematológicos y presencia de IgM en 92 pacientes que acudieron al IICS-UNA con sospecha clínica de dengue en el periodo 2009 al 2013. Se utilizó el MAC-ELISA desarrollado en el IICS-UNA, se registraron los datos clínicos-epidemiológicos a través de una encuesta y se determinaron los parámetros hematológicos. Se obtuvieron resultados positivos para IgM en 51/92 (55%) pacientes y resultados negativos en 41/92 (45%). Las características clínicas más frecuentes fueron: fiebre, cefalea, mialgias y artralgias. Entre los pacientes con IgM positiva, 14/51 (27%) manifestaron dolores abdominales, 19/51 (37%) reportaron letargo o postración y 20/51 (39%) declararon tener náuseas y/o vómitos, 4/51 (8%) presentaron leucopenia, 10/51 (20%) valores de hematocrito disminuido y 6/51 (12%) plaquetopenia. Sólo 13/92 (14%) pacientes declararon haber cursado con la enfermedad anteriormente. Del total de pacientes, 4/92 (4%) manifestaron haber presentado algún tipo de hemorragia. Los resultados obtenidos en el estudio refuerzan la importancia de integrar todos los parámetros posibles: detección de IgM, perfil hematológico y la clínica del paciente con sospecha de dengue para brindar un mejor diagnóstico. Así también, es necesario resaltar en cuanto a los signos de alarma para una intervención rápida a fin de evitar complicaciones. Dengue is one of the most important mosquito-borne diseases worldwide. The disease may present as asymptomatic, with a wide range of non-specific clinical manifestations or a certain percentage can result in severe cases. This observational, descriptive, cross-sectional study aimed to determine the clinical features, hematological parameters and the presence of IgM in 92 patients who attended to IICS-UNA with clinical suspicion of dengue from 2009 to 2013. The MAC-ELISA developed at IICS-UNA was used, clinical and epidemiological data were recorded through a survey and hematological parameters were determined. Positive results for IgM in 51/92 (55%) patients were obtained and negative results in 41/92 (45%). The most frequent clinical features were fever, headache, muscle and joint pains. Among patients with positive IgM, 14/51 (27%) reported abdominal pain, 19/51 (37%) reported lethargy or prostration and 20/51 (39%) reported having nausea and/or vomiting, 4/51(8%) had leucopenia, 10/51 (20%) decreased hematocrit values and 6/51 (12%) presented thrombocytopenia. Only 13/92 (14%) patients reported a previous DENV infection. Of the total, 4/92 (4%) presented some type of bleeding. The results of the study reinforce the importance of integrating all possible parameters: IgM detection, hematological and clinical profile of patients with suspected dengue to provide better diagnosis. It is also necessary to emphasize warning signs for a quick intervention to avoid complications.

The aim of the study was to determine the impact of triploidization on hematological and blood gas parameters in Siberian sturgeon, Acipenser baerii Brandt. The study material was comprised of juvenile Siberian sturgeon specimens with total body lengths of 22.3 ± 2.9 cm and mean body weights of 75.3 ± 40.7 g. Triploidization was confirmed to result in significant changes in the hematological profile of Siberian sturgeon. In comparison to the diploid group, the fish with higher levels of ploidy exhibited lower erythrocyte counts, increased hemoglobin concentration, and increased values of mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. A significant decrease in the percentage share of lymphocytes and an increase in the percentage share of granulocytes in the peripheral blood were also observed, which indicate disturbances in the immune response (particularly in the adaptive immune system) and greater susceptibility to stress. Triploidization did not, however, cause significant differences in the blood gas profile. CORRESPONDING AUTHOR: M. Rozynski [+] Department of Aquaculture Inland Fisheries Institute in Olsztyn Oczapowskiego 10, 10-719 Olsztyn, Poland Tel. +48 89 5241071; e-mail: maciej.rozynski@infish.com.pl K. Demska-Zakeś, D. Fopp-Bayat Department of Ichthyology University of Warmia and Mazury in Olsztyn, Poland

It was provide a hematological profile of Artibeus lituratus (Phyllostomidae: Stenodermatinae). Animals were collected from October 2017 to February 2018 in an urban forest in the city of Rio de Janeiro, Southeastern Brazil. Males and females showed similar overall mean values for the parameters analyzed. Males had higher values for erythrocytes (RBC), hematocrit and basophils. Females had higher levels of eosinophils than males. The hematological values reported here will serve as reference for future research on health conditions of free-living and captive populations of Artibeus lituratus, as well as for research on pathogens associated with these bats. O presente trabalho estabeleceu o perfil hematológico de morcegos frugívoros de vida livre, A. lituratus (Phyllostomidae: Stenodermatinae). As amostragens foram realizadas de outubro de 2017 a fevereiro de 2018 em uma floresta urbana na cidade do Rio de Janeiro, Sudeste do Brasil. Machos e fêmeas apresentaram valores médios gerais semelhantes para os parâmetros analisados. Os machos apresentaram valores de eritrócitos (RBC), hematócrito e basófilos mais altos. As fêmeas apresentaram níveis mais elevados de eosinófilos que os machos. Os valores hematológicos aqui reportados servirão de referência para pesquisas sobre condições de saúde de populações de Artibeus lituratus em vida livre e de cativeiro, assim como para pesquisas sobre patógenos associados a esses morcegos.

Preeclampsia is a specific syndrome in pregnancy that occur after 20 weeks gestation in women who were previously normotensive. Hypertension in pregnancy caused complications in cardiovascular, hematology, endocrine, metabolic and regional blood flow accompanied by disorders of various organs, resulting in an increase of hemoconcentration in haemoglobin. The purpose of this study was to analyze the hematology profile and birth weight in preeclampsia. the design of this study was cross sectional with a sample of 40 respondents, consisting of 20 preeclampsia women and 20 women normal pregnancy. The hematology profile assessed was haemoglobin levels, hematocrit levels, platelet levels and blood sedimentation rates. The results showed haemoglobin levels, hematocrit levels, platelet level and blood sedimentation rates in both groups were not significantly difference with p value in each category was 0,110; 0,163; 0,686 and 0,983 (p value >0,05). Thus this study concludes that the hematological profile and birth weight in preeclampsia and normal pregnancies are the same.

Background: A variety of hematological manifestations are seen at every stage of human immunodeficiency virus (HIV) infection, and they often pose a great challenge in the comprehensive management of acquired immunodeficiency syndrome. Anemia is the most common hematological abnormality associated with HIV infection. The severity and the incidence of cytopenia are usually correlated with the stage of the disease and underlying immune status if interpreted cautiously, especially if the patient is on regular follow-up. The primary objective of the present study was to understand the spectrum of hematological abnormalities in HIV-infected patients, whereas the secondary objective was to evaluate the correlation of hematological abnormalities with absolute CD4 count and HIV viral load. Materials and Methods: The present cross-sectional descriptive study was conducted on 100 patients, aged 18 years and above, diagnosed with HIV infection and confirmed by Western blot or ELISA method. Both inpatients and outpatients at our tertiary care hospital were included in the study. Results: Individuals with high viral load and low CD4 count had a higher prevalence of anemia. There was a statistically significant and directly proportionate decrease in the absolute CD4 count as the hemoglobin levels decreased (P = 0.004). In the present study, normocytic normochromic blood picture and anemia of chronic disease blood picture were more prevalent among the study participants. Individuals with high viral load and CD4 count

Haematological and biochemical parameters were examined in the blood of 87 cows, bulls and oxen of the indigenous Croatian breed, Istrian cattle. The sample represents 11.93 % of the total adult population. This breed is classified as highly endangered. The age of tested animals was between 2 and 17 years. Cattle were divided into three groups, animals aged 2 to 6 years (n=19), 7 to 10 years (n=37), and older than 10 years (n=31). Animals were kept in the area of Istria County, mostly in stables though occasionally at pasture. The cows were milked twice daily and gave an average of 1 000 litres of milk per year. Erythrocytes, leukocytes, haemoglobin, haematocrit and mean corpuscular volume were determined. Differential analysis was performed by counting blood cells in the blood smear after Pappenheim staining. Biochemical parameters such as total cholesterol, triglycerides, urea, creatinine, total bilirubin, calcium and inorganic phosphorus were determined. The activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase was determined by spectrophotometry. These biochemical and haematological parameters of this Istrian breed were within the physiological range, while slight deviations were observed for certain parameters. The greatest deviation from the physiological range was shown in the concentrations of urea and creatinine. These results may contribute to a better understanding of the metabolic profile and haematological indicators for estimating the physiological status of these endangered cattle in Istria, for further investigation and for diagnostic purposes.

Background: Immunity system plays important roles in maintaining health status in human as well as in animals.This work aims to study the effect of supplementation of food supplement Viusid vet 90 on growth performance, morbidity and mortality rates, hematological profile, and on antibody titer which may indicate its possible role as an immunomodulator. Methods: Sixty (60) piglets were used as the experimental animals divided into 2 groups, namely group without Viusid supplementation as the control group and that with Viusid supplementation starting from 3 days of age until 13 days of age (10 days of treatment). The dose of supplementation in the feed was 5 cc of Viusid given twice a day. At day 7 of age, the piglets were vaccinated intramuscularly with Hog cholera vaccine and it repeated at day 14 of age. The animals were weighed at the beginning of the experiment and again at Day 15 and then at the end the experiment at Day 30. At 20 days of age all animals were bled using vacuum tube containing anticoagulant EDTA. Subsequently the bloods were examined for hematological profiles using Manual ABC VET Automated blood counter. Finally, at 30 days of age they were all bled again for the assessment of their antibody titer against Hog cholera following ELISA method. Results: supplementation of Viusid vet 90 to piglets improves performance by reducing morbidity and mortality rates. As hematological profile and differential leucocyt count did not showed any abnormality then it may concluded that supplementation of Viusid is effective and safe for piglets. Moreover, the percentage Plate Count (PC) value for Hog cholera was seropositive (54.69%) and significantly higher than control (41.32%) and this could indicate that Viusid vet 90 act as a strong immunmodulator.

The aim of this study was to assess the changes in hematological and plasma indices of Ctenopharyngodon idella (Val.) with regard to age, sex, and hormonal treatment. Sixty specimens of C. idella, comprising fifteen of each age (6, 12, 24, and 36 months) were used to evaluate the age factor. Additionally, 20 fish, both females and males, were designated to determine blood indices with regard to sex and hormonal treatment. The following biochemical indexes were determined in the blood plasma: glucose, cholesterol, triglycerides, and total protein (TP). Glucose and triglyceride levels differed significantly among ages, and significant age-related differences were noted in the values of red blood cell (RBC), white blood cell (WBC), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC). There were no significant differences in any parameters between males and females. In conclusion, our results showed that the plasma parameter profile could be affected by age (RBC, WBC, Hb, MCV, MCH, MCHC, glucose, triglycerides) and hormonal treatment (lymphocytes and eosinophils). The blood plasma biochemical and hematological profiles permitted assessing physiological state, which provided useful information for monitoring changes in the health status of the fish.

The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage of CD4+ and CD8+ lymphocytes in Ehrlich tumor-bearing mice. The treatment with the ABM extract in water bath and ultrasound at pH 4 resulted in lower lymphocyte counts, regardless of tumor presence, and greater granulocyte values in mice with Ehrlich tumor than in controls. We concluded that different fractions and methods of extraction of A. blazei produced differing blood profiles in mice inoculated with the Ehrlich tumor. O objetivo deste estudo foi investigar o efeito de diferentes extratos do cogumelo Agaricus blazeii Murril (ABM) sobre o perfil hematológico de camundongos Swiss portadores de tumor de Ehrlich sólido. Três frações (extrato total, polissacarídeos e sobrenadante) dos extratos de ABM foram obtidas por quatro métodos (sonificador, banho-maria, em pH 4 ou pH 7) e administradas para camundongos durante 21 dias. Soluções de polissacarídeos foram analisadas por cromatografia gasosa e líquida, que mostraram concentrações de glucose e manose. O método de extração influenciou o grau de polimerização da glicose e a relação manose/glucose. O tratamento com o sobrenadante de ABM (em pH 7 e banho-maria) estava associado com reduzidas concentrações de leucócitos e linfócitos, além de alterar a porcentagem de linfócitos CD4+ e CD8+ em camundongos portadores de tumor sólido de Ehrlich. O tratamento com extratos de ABM, obtidos tanto em banho-maria como no sonificador em pH 4, resultou nas mais baixas contagens de linfócitos, independentemente da presença do tumor, e nos maiores valores de granulócitos em camundongos com tumor de Ehrlich. Conclui-se que os diferentes métodos de extração com as respectivas frações de A. blazei são capazes de intereferir no perfil hematológico de camundongos com tumor sólido de Ehrlich.

Background: Acromegaly is a disease characterized by growth hormone and insulin like growth factor hypersecretion due mostly to pituitary somatotropic adenoma. The diagnosis of Acromegaly is usually delayed for years exposing patients to slowly evolving chronic complications. Objectives: To explore the value of performing peripheral blood examination as routine work up in monitoring Iraqi patients with Acromegaly. Patients and Methods: This study was conducted on 38 patients with Acromegaly attending the national center for diabetes research and management. Peripheral blood indices were done by hematological analyzer and blood film stained by Gemisa stain for proper cells morphology done at hematological unit in Iraqi center for cancer and genetics research. Results: The patients examined showed higher values compared with control group in platelets indices (MPV and PDW) that were statistically non-significant. The monocyte count was significantly lower in patients compared by control group (p

El benceno, es un hidrocarburo aromático con alto riesgo toxicológico, por lo cual el monitoreo biológico de los trabajadores expuestos, es de gran importancia para prevenir daños a la salud. Se evaluaron niveles de benceno urinario (BU), estrés oxidativo, perfi l hematológico, hepático y renal en trabajadores de estaciones de servicio (zona norte de Valencia-Venezuela). Se trató de un estudio no experimental, descriptivo-correlacional. Participaron 100 trabajadores: 60 expuestos (E) y 40 no expuestos (NE). Se determinó BU por cromatografía de gases acoplada a masas, en muestras de orina obtenidas al fi nal de la jornada laboral y en sangre fueron analizados: malondialdehído (MDA), vitamina C (VC), perfi l hematológico, hepático y renal. Los datos presentados como frecuencias, porcentajes, medianas, medias y desviación estándar, mostraron: valores de BU en 17 trabajadores y estos aumentaban según su ocupación. Los valores de MDA fueron signifi cativamente más altos en E (p

Background: The association between the haematological profile (including abnormal platelets) and malaria is not completely understood. There are few published data on haematological profiles of malaria patients in areas with unstable malaria transmission. The current study was conducted to investigate if the haematological parameters, including platelet indices, were reliable predictors for microscopically-diagnosed malaria infection. Methods: A case-control study with a total of 324 participants (162 in each arm) was conducted at the out-patient clinic of New Halfa hospital during the rainy and post rainy season (August 2014 through to January 2015). The cases were patients with uncomplicated Plasmodium falciparum (107; 66.9%) and P. vivax malaria (55, 34.0%) infections. The controls were aparasitemic individuals. The haematological parameters were investigated using an automated hemo-analyser. Results: There was no significant difference in the mean (±SD) age between the study groups; however, compared to the controls, patients with uncomplicated malaria had significantly lower haemoglobin, leucocyte and platelet counts, and significantly higher red cell distribution width (RDW), platelet distribution width (PDW) and mean platelet volume (MPV). Conclusions: The study revealed that among the haematological indices, PDW and MPV were the main predictors for uncomplicated P. falciparum and P . vivax malaria infection . Abbreviations: OR: odds ratio.

The knowledge of hematological values is useful in diagnosing various pathological and metabolic disorders, which can adversely affect reproductive performance of cows. The aim of this study was to investigate body condition score (BCS), body weight, calf weight, blood erythrocyte parameters and total and differential leukocyte count changes in dystocia-affected crossbred dairy cows. Values of mean corpuscular volume (MCV) and neutrophil count were markedly increased (P

BACKGROUND: Thousands of pregnant women are infected with human immunodeficiency virus (HIV) and some of them take antiretroviral therapy (ART) either for their own health or as a means of preventing mother-to-child transmission. This entails fetal exposure to drugs with attendant effect on hematological parameters. AIMS: The aim of this study is to determine the effect of maternal HIV and ART on hematological profile of newborns. SETTINGS AND DESIGN: Comparative cross-sectional study involving 70 each of HIV- and ART-exposed and HIV- and ART-unexposed newborns at Aminu Kano Teaching Hospital, Kano, Nigeria. SUBJECTS AND METHODS: Cord blood was collected for hemogram, reticulocyte count, and erythrocyte sedimentation rate. STATISTICAL ANALYSIS USED: Data were analyzed using SPSS version 20 and P < 0.05 was considered statistically significant. RESULTS: The mean hematocrit, platelet, and reticulocyte counts of the HIV-exposed newborns were significantly lower than those of HIV-unexposed (P < 0.05). Among HIV-exposed newborns, newborns of mothers with CD4+ T-cell

The green turtle Chelonia mydas feeds and nests in the Brazilian coastal area and is considered an endangered species by the World Conservation Union (IUCN 2009) and threatened by the Red List of Brazilian Fauna (Ministério do Meio Ambiente 2009). Fibropapillomatosis is a disease characterized by benign skin tumors (fibropapillomas), and it is one of the main threats to the survival of this species. Studies suggest the involvement of viruses as infectious agents associated with environmental and genetic factors. Blood samples were collected from 45 turtles captured in the coastal area of the state of Sao Paulo, Brazil. From these, 27 were affected by fibropapillomas and 18 were tumor free. Biometrical data on the turtles, size, location and quantity of tumors were recorded. The area occupied by fibropapillomas per animal was calculated and four groups were determined according to severity of the disease or its absence. The objective of the study was to compare hemogram results of the sea turtles classified in these four groups. The lowest hematocrit value was observed in severely affected animals. In the hemoglobin assay, the highest value was observed in the group of tumor free turtles and the lowest, in animals severely affected. Lymphocyte counts and curved carapace length were on the verge of statistical significance.Chelonia mydas, denominada tartaruga verde, é uma tartaruga marinha que frequenta o litoral brasileiro para alimentação e nidificação e é considerada em perigo de extinção pela IUCN (World Conservation Union, 2009) e ameaçada pela Lista Vermelha da Fauna Brasileira (Ministério do Meio Ambiente, 2009). A fibropapilomatose, doença caracterizada por tumores cutâneos benignos (fibropapilomas), é uma das mais importantes ameaças à sobrevivência dessa espécie. Pesquisas sugerem o envolvimento de agentes infecciosos virais em associação com fatores ambientais e genéticos. Foram colhidas amostras sanguíneas de 45 tartarugas provenientes do litoral do estado de São Paulo, Brasil, sendo 18 sem fibropapilomas e 27 acometidas. Dados de biometria das tartarugas, quantidade, localização e tamanho dos tumores foram anotados. Foi realizado o cálculo da área de fibropapilomas por animal e foram estipulados 4 grupos de acordo com o grau de acometimento e sua ausência. O objetivo foi realizar uma comparação entre os hemogramas das tartarugas marinhas classificadas nos 4 grupos. Animais de grau grave apresentaram o menor valor para hematócrito. Para dosagem de hemoglobina, observou-se que o maior valor foi para o grupo de tartarugas sem fibropapilomas e o menor para o de grau grave. Os valores de linfócitos e comprimento curvilíneo da carapaça beiraram a significância estatística.