1. Divergent roles for KLF4 and TFCP2L1 in Naive Ground State Pluripotency and Human Primordial Germ Cell Development
- Author
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Grace V. Hancock, Wanlu Liu, Joanna J. Gell, Amanda J. Collier, Di Chen, Kathrin Plath, Jesse R. Zamudio, Amander T. Clark, and Lior Peretz
- Subjects
Pluripotency ,Cell type ,QH301-705.5 ,1.1 Normal biological development and functioning ,Human Embryonic Stem Cells ,TFCP2L1 ,Stem cells ,Biology ,hPGCs ,Medical and Health Sciences ,Article ,Kruppel-Like Factor 4 ,Underpinning research ,Gene expression ,Genetics ,Humans ,Biology (General) ,Stem Cell Research - Embryonic - Human ,Transcription factor ,Embryogenesis ,PGCs ,Primordial Germ Cells ,Cell Differentiation ,Cell Biology ,General Medicine ,Biological Sciences ,Stem Cell Research ,Embryonic stem cell ,KLF4 ,Cell biology ,Repressor Proteins ,Germ Cells ,Epiblast ,embryonic structures ,Generic health relevance ,Stem cell ,Transcriptome ,Developmental Biology ,Transcription Factors - Abstract
During development, human primordial germ cells (hPGCs) transition through a transcriptional and epigenetic state similar to pre-implantation naive ground state epiblast cells. In hPGCs, this state is called naive-like ground state pluripotency. Diagnostic transcription factors that define this state include TFAP2C, KLF4, and TFCP2L1, with TFAP2C necessary for both establishment of the naive-like ground state in hPGC-like cells (hPGCLCs) and establishment of naive ground state human embryonic stem cells (hESCs). Here, we show that KLF4 and TFCP2L1 are not required for hPGC specification or establishment of the naive-like ground state in hPGCLCs. Instead, KLF4 and TFCP2L1 are each required for reversion of primed hESCs to the self-renewing naive ground state. Additionally, TFCP2L1 but not KLF4 function after hPGC specification in the proliferation and survival of hPGCLCs.
- Published
- 2020
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