1. Studies on lysophospholipases
- Author
-
de Jong Jg, A.J. Aarsman, van den Bosch H, and van Deenem Ll
- Subjects
chemistry.chemical_classification ,Chromatography ,Butanol ,Biophysics ,Fatty acid ,Biochemistry ,chemistry.chemical_compound ,Hydrolysis ,Endocrinology ,Enzyme ,chemistry ,Lysophospholipase ,Sephadex ,Phosphatidylcholine ,Sodium dodecyl sulfate - Abstract
1. 1. Lysophospholipase was purified from homogenates of fresh beef pancreas by acid treatment, (NH 4 ) 2 SO 4 precipitation in the presence of n -butanol and ion-exchange chromatography using SE-Sephadex C-50 and DEAE-Sephadex A-50. 2. 2. The molecular weight of the nearly homogeneous enzyme was estimated to be 65000 from sodium dodecyl sulfate disc electrophoresis and Sephadex G-200 filtration. 3. 3. The products of the hydrolysis of 1-acyl-glycerylphosphorylcholine containing long chain acyl groups were shown to be free fatty acid and glycerylphosphorylcholine, whereas long chain phosphatidylcholine was not attacked at all. 4. 4. The enzyme did not require bivalent metal ions and was unaffected by SH-group reagents. Diisopropylfluorophosphate in I mM concentration completely abolished enzymatic activity.
- Published
- 1973