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Background:CYP2C19 is an important genetic factor modulating clopidogrel dose requirement.Objective:Therefore, a simple and economic genotyping method for predicting the clopidogrel dose of patients would be useful in clinical applications.Methods:In this study, the MGB blocker ARMS real-time PCR contained two forward primers, two MGB blockers and a common reverse primer have been used for CYP2C19*2, *3 and *17 substitutions.Results:Results showed that heterozygotes and homozygotes of CYP2C19*2, *3 and *17 could be distinguished by the MGB blocker ARMS real-time PCR successfully. In the Chinese population, patients with allele frequencies of CYP2C19*2, *3, and *17 are 18.43%, 3.03% and 0.76%, respectively.Conclusion:This study indicates that the MGB blocker ARMS real-time PCR will be a simple, economical method for the rapid detection of SNPs in CYP2C19.

The equipment manufacturing industry is the basic and strategic industry which is related to the long-term interests of the country. Innovation ability is an important symbol to measure the comprehensive competitiveness of a country or region. In this paper, the evaluation index system of collaborative innovation ability of equipment manufacturing enterprises is constructed from three aspects: innovation input, innovation cooperation and innovation output. Then, the evaluation model is established by factor analysis method. In the empirical analysis process of the equipment manufacturing enterprises collaborative innovation ability, the panel data of eight industries in China's equipment manufacturing industry from 2009 to 2014 is used as the research sample, and makes a comparative study on the collaborative innovation ability within the equipment manufacturing industry. Finally, hope to provide the basis for enterprises to enhance collaborative innovation and develop more scientific and innovative decision-making.