1. Circ-APBB1IP as a Prognostic Biomarker Promotes Clear Cell Renal Cell Carcinoma Progression Through The ERK1/2 Signaling Pathway
- Author
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Lixin Chen, Haokai Wu, Yuwan Zhao, Shanhong Lin, Zhixian Ao, Jianjun Liu, Wenfeng Zeng, and Jierong Mo
- Subjects
MAP Kinase Signaling System ,Apoptosis ,clear cell renal cell carcinoma ,medicine.disease_cause ,circ-APBB1IP ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Annexin ,Circular RNA ,medicine ,Humans ,Fluorescein isothiocyanate ,Carcinoma, Renal Cell ,Cell Proliferation ,Gene knockdown ,Wound Healing ,Chemistry ,General Medicine ,Transfection ,RNA, Circular ,medicine.disease ,Flow Cytometry ,Prognosis ,ERK1/2 signaling pathway ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,Clear cell renal cell carcinoma ,Cancer research ,Disease Progression ,030211 gastroenterology & hepatology ,progression ,Carcinogenesis ,Research Paper ,Signal Transduction - Abstract
Circular RNA (circRNA), a member of non-coding RNA, plays an essential regulatory role in many human physiological and pathological processes; however, its role in clear cell renal cell carcinoma (ccRCC) still unclear. This study aims to investigate the effect and mechanisms of circRNA on ccRCC progression. A human circRNA microarray was used to discover differential expression circRNA, and a quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the expression of circRNA. The function and mechanism of circRNA were explored by cell transfection, cell counting kit-8, fluorescein isothiocyanate (FITC) Annexin V apoptosis detection, wound healing, transwell, and western blot. The result indicated that circ-APBB1IP was significantly up-regulated in ccRCC. In vitro, knockdown of circ-APBB1IP by siRNA suppressed the proliferation, migration, and invasion and increased the apoptosis of ccRCC cells. Further study found that knockdown of circ-APBB1IP up-regulated protein expression of cleaved caspase-3, cleaved caspase-7, cleaved caspase-8, cleaved caspase-9, Bax, Bad, Bak, E-cadherin and down-regulated expression of Bcl-2, N-cadherin, MMP-2, MMP-9, p-ERK1/2. Our result indicates that circ-APBB1IP has a vital function in ccRCC tumorigenesis. These findings suggest that circ-APBB1IP represents a novel potential biomarker and therapeutic target of ccRCC.
- Published
- 2020