14 results on '"Zhihao Dai"'
Search Results
2. Low depression literacy exacerbates the development and progression of depressive mood in Chinese adult social media users during COVID-19: A 3-month observational online questionnaire-based study with multiple cross-sectional analyses
- Author
-
Dan Shan, Shaoyang Li, Ruichen Xu, Jingtao Huang, Yi Wang, Yuandian Zheng, Shanshan Huang, Yuming Song, Junchu Han, Sayaka Suto, and Zhihao Dai
- Subjects
Public Health, Environmental and Occupational Health - Abstract
BackgroundThe main purpose of this study was to explore the relationship between depression literacy (D-Lit) and the development and progression of depressive mood.MethodsThis longitudinal study with multiple cross-sectional analyses used data from a nationwide online questionnaire administered via the Wen Juan Xing survey platform. Eligible participants were 18 years or older and had subjectively experienced mild depressive moods at the time of their initial enrollment in the study. The follow-up time was 3 months. Spearman's rank correlation test was used to analyze the predictive role of D-Lit on the later development of depressive mood.ResultsWe included 488 individuals with mild depressive moods. No statistically significant correlation between D-Lit and Zung Self-rating Depression Scale (SDS) at baseline was observed (adjusted rho = 0.001, p = 0.974). However, after 1 month (adjusted rho = −0.449, p < 0.001) and after 3 months (adjusted rho = −0.759, p < 0.001), D-Lit was significantly and negatively correlated with SDS.LimitationsThe targeted subjects were limited to the Chinese adult social media users; meanwhile, China's current management policies for COVID-19 differ from most of the other countries, limiting the generalizability of this study.ConclusionDespite the limitations, our study provided novel evidence supporting that low depression literacy may be associated with exacerbated development and progression of depressive mood, which, if not appropriately and promptly controlled, may ultimately lead to depression. In the future, we encourage further research to explore the practical and efficient ways to enhance public depression literacy.
- Published
- 2023
3. Subjective attitudes moderate the social connectedness in esports gaming during COVID-19 pandemic: A cross-sectional study
- Author
-
Dan Shan, Jilai Xu, Tongyu Liu, Yanyi Zhang, Ziyun Dai, Yuandian Zheng, Chang Liu, Yuanning Wei, Zhihao Dai, and Apollo - University of Cambridge Repository
- Subjects
communication frequency ,public mental health ,Adolescent ,Public Health, Environmental and Occupational Health ,COVID-19 ,social connection ,Young Adult ,Cross-Sectional Studies ,Attitude ,Video Games ,online game ,Communicable Disease Control ,Humans ,Pandemics - Abstract
Peer reviewed: True, Acknowledgements: The authors thank the professional online survey platform-Credamo for data provided in this article., BACKGROUND: Electronic sports (esports) has become a practical intervention for young people craving social connections since the COVID-19 pandemic. Past studies have shown an equivocal role of esports participation in boosting social ties or social connectedness. It is unclear if their relationship is affected by subjective attitudes of gamers. Moreover, the present COVID-19 pandemic may further modify this relationship to a greater extent. OBJECTIVE: This study primarily aimed to investigate the moderating effect of participants' subjective attitudes toward esports gaming on the relationship between in-game interaction during esports participation and participants' anticipated social connectedness among Chinese young adults during the COVID-19 lockdown periods in China. METHODS: We conducted a nationwide online questionnaire survey through the Credamo platform among 550 Chinese young adults in the present study. The Social Connectedness Scale-Revised was used to assess participants' social connectedness levels. RESULTS: Four hundred and fifty-three participants were included in the final analysis. The effective response rate was 82.4%. Our results showed that the esports participation measured by in-game communication frequency among participants, as an independent factor, was negatively associated with participants' social connectedness scores (β = -0.13, p < 0.05). However, when the moderating effect of subjective attitudes toward esports gaming was considered, the association between communication frequency and social connectedness scores was turned into the opposite direction with a larger effect size (β = 0.35, p < 0.001). CONCLUSION: Our primary finding revealed that a positive mindset in esports gaming is indispensable in boosting social connectedness. Overall, our study provided supporting evidence for the benefits of esports on individuals' social connectedness. In future circumstances similar to the COVID-19 era, playing esports games is strongly encouraged in an attempt to maintain social connections and relieve psychological stress. In the meantime, we believe that having a positive esports experience, often associated with a positive mindset during gaming, can better promote social connectedness. Nevertheless, the amount of time spent on gaming per day should be of great concern, as esports games can be addictive, especially for teenagers and college students.
- Published
- 2023
- Full Text
- View/download PDF
4. A Heterogeneous Redundant Architecture for Industrial Control System Security
- Author
-
Zhihao Dai, Matthew Leeke, Yulong Ding, and Shuang-hua Yang
- Published
- 2022
5. Post-COVID-19 human memory impairment: A PRISMA-based systematic review of evidence from brain imaging studies
- Author
-
Dan Shan, Shaoyang Li, Ruichen Xu, Glen Nie, Yangyiran Xie, Junchu Han, Xiaoyi Gao, Yuandian Zheng, Zhen Xu, and Zhihao Dai
- Subjects
Aging ,Cognitive Neuroscience - Abstract
Many people with coronavirus disease 2019 (COVID-19) report varying degrees of memory impairment. Neuroimaging techniques such as MRI and PET have been utilized to shed light on how COVID-19 affects brain function in humans, including memory dysfunction. In this PRISMA-based systematic review, we compared and summarized the current literature looking at the relationship between COVID-19-induced neuropathological changes by neuroimaging scans and memory symptoms experienced by patients who recovered from COVID-19. Overall, this review suggests a correlational trend between structural abnormalities (e.g., cortical atrophy and white matter hyperintensities) or functional abnormalities (e.g., hypometabolism) in a wide range of brain regions (particularly in the frontal, parietal and temporal regions) and memory impairments in COVID-19 survivors, although a causal relationship between them remains elusive in the absence of sufficient caution. Further longitudinal investigations, particularly controlled studies combined with correlational analyses, are needed to provide additional evidence.
- Published
- 2022
6. Research on Hierarchical SDG Modeling Method of Flight Control System Based on FSM and Output Constraint
- Author
-
Zhihao Dai, Jianwei Liu, Jianhao Xing, and Pu Yang
- Published
- 2022
7. Axenfeld-Rieger syndrome: a novel histopathologic finding associated with corneal abnormalities
- Author
-
Ting Yu, Zhihao Dai, Rongmei Peng, Gege Xiao, Pei Zhang, Siyi Ma, and Jing Hong
- Subjects
Ophthalmology ,Corneal Opacity ,Iris Diseases ,Anterior Eye Segment ,Humans ,Endothelial Cells ,General Medicine ,Eye Abnormalities ,Retrospective Studies ,Corneal Diseases - Abstract
Background Axenfeld-Rieger syndrome (ARS) is a rare kind of anterior segment dysgenesis (ASD). The most common ocular features of ARS are posterior embryotoxon and iris hypoplasia, while some patients may manifest as corneal opacity and edema. However, the current understanding of how ARS affects the cornea is still incomplete. This study reports a novel histopathological finding of ARS, complicating corneal abnormalities, including congenital corneal opacity and irreversible endothelial decompensation. Methods This retrospective study included 6 eyes of 3 ARS patients, 5 of which underwent keratoplasty for irreversible endothelial decompensation from May 2016 to January 2019. No eye had a history of surgery. We reviewed the data of epidemiology, clinical manifestations and histopathologic examinations. Results Five eyes developed irreversible endothelial decompensation, among which 4 were born with corneal opacity. One eye exhibited transparent cornea but showed a continuous loss of endothelial cells in the absence of surgery and elevated intraocular pressure thereafter. Anterior segment optical coherence tomography photographs showed that anterior synechia existed in the area with corneal opacities, where we found the interlayer splitting of the Descemet membrane inserted by hypoplastic iris and a basement membrane-like structure under a light microscope. Conclusion Anterior synechia might be associated with corneal abnormalities in ARS patients. The novel histopathologic finding revealed the internal relation between anterior segment dysgenesis and would help explore the inner mechanism of corneal abnormalities in ARS.
- Published
- 2022
8. Experimental investigation of a reinforced soil retaining wall with a flexible geogrid-wrapped ecological bag facing
- Author
-
Junyi Duan, Guo-lin Yang, Yu-liang Lin, Xinting Cheng, and Zhihao Dai
- Subjects
010102 general mathematics ,0211 other engineering and technologies ,Oblique case ,Distribution law ,02 engineering and technology ,Geotechnical Engineering and Engineering Geology ,Retaining wall ,01 natural sciences ,Geogrid ,Lateral earth pressure ,General Materials Science ,Geotechnical engineering ,0101 mathematics ,Geology ,021101 geological & geomatics engineering ,Civil and Structural Engineering ,Degree Rankine - Abstract
This paper presented a field study of the reinforced soil wall (RSW) with a geogrid wrap-around facing. In addition to the conventional monitoring content, the strain of the face-wrapping geogrid, which was neglected in most previous studies, was monitored during the construction process. The positional relationship between the maximum vertical earth pressure and horizontally laid geogrid strain was revealed by using the proposed oblique dragging effect. It was found that the strain on the face-wrapping geogrid occurred mainly in the early stage of construction. The oblique dragging effect existing in the flexible RSWs increased the vertical earth pressure and changed its distribution law, resulting in the position of the maximum vertical soil pressure appearing behind the position of the maximum horizontally laid geogrid strain. The horizontal earth pressure at different positions behind the wall was obviously lower than the theoretical result by using Rankine's theory. Also, a modified 0.3H method (where H refers to the wall height) was presented to account for the slope of the wall face, which could be used to determine the potential sliding surfaces for single-stage RSWs and two-tier RSWs with small offset.
- Published
- 2021
9. Differential Evolution Algorithm Optimized ELM Fault Diagnosis of Flight Control System Actuator
- Author
-
Yifan Wang, Jianwei Liu, Jianhao Xing, and Zhihao Dai
- Published
- 2021
10. BoneBert: A BERT-based Automated Information Extraction System of Radiology Reports for Bone Fracture Detection and Diagnosis
- Author
-
Zhihao Dai, Zhong Li, and Lianghao Han
- Subjects
medicine.medical_specialty ,Decision support system ,Computer science ,business.industry ,Radiography ,02 engineering and technology ,Bone fracture ,medicine.disease ,computer.software_genre ,Information extraction ,Named-entity recognition ,020204 information systems ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,020201 artificial intelligence & image processing ,Radiology ,Medical diagnosis ,business ,computer ,Encoder ,Fracture type - Abstract
Radiologists make the diagnoses of bone fractures through examining X-ray radiographs and document them in radiology reports. Applying information extraction techniques on such radiology reports to retrieve the information of bone fracture diagnosis could yield a source of structured data for medical cohort studies, image labelling and decision support concerning bone fractures. In this study, we proposed an information extraction system of Bone X-ray radiology reports to retrieve the details of bone fracture detection and diagnosis, based on a bio-medically pre-trained Bidirectional Encoder Representations from Transformers (BERT) natural language processing (NLP) model by Google. The model, named as BoneBert, was first trained on annotations automatically generated by a handcrafted rule-based labelling system using a dataset of 6,048 X-ray radiology reports and then fine-tuned on a small set of 4,890 expert annotations. Thus, the model was trained in a “semi-supervised” fashion. We evaluated the performance of the proposed model and compared it with the conventional rule-based labelling system on two typical tasks: Assertion Classification (AC) for bone fracture status detection (positive, negative or uncertainty) and Named Entity Recognition (NER) related to the fracture type, the bone type and location of a fracture occurs. BoneBert outperformed the rule-based system in both tasks, showing great potential for automated information extraction of the detection and diagnosis of bone fracture from radiology reports, such as, the clinical status, type and location of bone fracture, and more related observations.
- Published
- 2021
11. Hypoxic stimulation of vascular endothelial growth factor expression in activated rat hepatic stellate cells
- Author
-
Zhihao Dai, Victor Ankoma-Sey, and Yun Wang
- Subjects
Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Cell signaling ,Liver cytology ,Gene Expression ,Endothelial Growth Factors ,Biology ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Nitric Oxide Donors ,Receptors, Growth Factor ,RNA, Messenger ,Carbon Tetrachloride ,Cells, Cultured ,Lymphokines ,Hepatology ,Reverse Transcriptase Polymerase Chain Reaction ,Vascular Endothelial Growth Factors ,Penicillamine ,Receptor Protein-Tyrosine Kinases ,Cell Hypoxia ,Rats ,Cell biology ,Oxygen ,Vascular endothelial growth factor ,Alternative Splicing ,Kinetics ,Vascular endothelial growth factor A ,Receptors, Vascular Endothelial Growth Factor ,Endocrinology ,medicine.anatomical_structure ,Liver ,chemistry ,Cell culture ,Hepatocyte ,Hepatic stellate cell ,Wound healing - Abstract
The tissue repair response to hypoxic stimuli during wound healing includes enhanced production of angiogenic factors, such as vascular endothelial growth factor (VEGF). Hepatic stellate cells are oxygen-sensing cells, capable of producing VEGF. We hypothesized that hypoxia-stimulated signaling in activated stellate cells mediate VEGF secretion during liver injury. The specific aim was to evaluate the effect of hypoxia on the gene expression of VEGF in HSC-T6 cells, an immortalized rat hepatic stellate cell line, and in rat primary cultures of stellate cells. Hypoxic induction of VEGF mRNA was dose- and time-dependent. The hypoxic stimulation of VEGF messenger RNA (mRNA) correlated with the secretion of VEGF protein in conditioned media by hypoxic T6 cells. S-Nitroso-N-acetyl-D, L-penicillamine (SNAP), a nitric oxide (NO) donor, and desferrioxamine (DFx) and cobalt chloride, mimics of cellular hypoxia, similarly stimulated VEGF mRNA expression and secretion. Four previously described splice variants of the VEGF mRNA (VEGF-120, 144, 164, 188) were detected in both normoxic- or hypoxic-activated stellate cells. There was differential expression of the VEGF receptors, Flt-1 and Flk-1, in hypoxic T6 cells. Hypoxic conditions selectively stimulated Flt-1 mRNA expression, whereas Flk-1 mRNA remained unchanged. Hypoxic induction of VEGF was also demonstrated in primary stellate cell cultures and after in vivo injury. Hypoxia stimulates cell signaling in stellate cells, culminating in the rapid induction of VEGF and Flt-1 mRNA expression and VEGF secretion. The hypoxic induction of VEGF is mimicked by NO and may be of mechanistic importance in the pathogenesis of hepatic wound healing and hepatocarcinogenesis.
- Published
- 2000
12. Regulation of anthrax toxin activator gene (atxA) expression in Bacillus anthracis: temperature, not CO2/bicarbonate, affects AtxA synthesis
- Author
-
Zhihao Dai and Theresa M. Koehler
- Subjects
Transcriptional Activation ,Anthrax toxin ,Bacterial Toxins ,Immunoblotting ,Immunology ,medicine.disease_cause ,Microbiology ,Genes, Regulator ,Gene expression ,Escherichia coli ,medicine ,Regulator gene ,Regulation of gene expression ,Antigens, Bacterial ,biology ,Activator (genetics) ,Toxin ,Temperature ,Gene Expression Regulation, Bacterial ,Carbon Dioxide ,biology.organism_classification ,Molecular biology ,Bacillus anthracis ,Bicarbonates ,Infectious Diseases ,Parasitology ,Research Article - Abstract
Anthrax toxin gene expression in Bacillus anthracis is dependent on the presence of atxA, a trans-acting regulatory gene located on the resident 185-kb plasmid pXO1. In atxA+ strains, expression of the toxin genes (pag, lef, and cya) is enhanced by two physiologically significant signals: elevated CO2/bicarbonate and temperature. To determine whether increased toxin gene expression in response to these signals is associated with increased atxA expression, we monitored steady-state levels of atxA mRNA and AtxA protein in cells cultured in different conditions. We purified histidine-tagged AtxA [AtxA(His)] from Escherichia coli and used anti-AtxA(His) serum to detect AtxA in protein preparations from B. anthracis cells. AtxA was identified as a protein with an apparent size of 56 kDa in cytoplasmic fractions of B. anthracis cells. Our data indicate that atxA expression is not influenced by CO2/bicarbonate levels. However, the steady-state level of atxA mRNA in cells grown in elevated CO2/bicarbonate at 37 degrees C is five- to sixfold higher than that observed in cells grown in the same conditions at 28 degrees C. A corresponding difference in AtxA protein was also seen at the different growth temperatures. When atxA was cloned on a multicopy plasmid in B. anthracis, AtxA levels corresponding to the atxA gene copy number were observed. However, this strain produced significantly less pag mRNA and protective antigen protein than the parental strain harboring atxA in single copy on pXO1. These results indicate that increased AtxA expression does not lead to a corresponding increase in pag expression. Our data strongly suggest that an additional factor(s) is involved in regulation of pag and that the relative amounts of such a factor(s) and AtxA are important for optimal toxin gene expression.
- Published
- 1997
13. The atxA gene product activates transcription of the anthrax toxin genes and is essential for virulence
- Author
-
Zhihao Dai, Jean-Claude Sirard, Michèle Mock, and Theresa M. Koehler
- Subjects
Transcriptional Activation ,Anthrax toxin ,Mutant ,Bacterial Toxins ,Molecular Sequence Data ,Microbiology ,Gene product ,Mice ,Sequence Homology, Nucleic Acid ,Gene expression ,Endoribonucleases ,Animals ,RNA, Antisense ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,DNA Primers ,Antigens, Bacterial ,biology ,Base Sequence ,Virulence ,Structural gene ,Promoter ,biology.organism_classification ,Molecular biology ,Bacillus anthracis ,Mutagenesis, Insertional ,Trans-Activators ,Gene Deletion - Abstract
Bacillus anthracis plasmid pXO1 carries the structural genes for the three anthrax toxin proteins, cya (edema factor), lef (lethal factor), and pag (protective antigen). Expression of the toxin genes by B. anthracis is enhanced during growth under elevated levels of CO2. This CO2 effect is observed only in the presence of another pXO1 gene, atxA, which encodes a transactivator of anthrax toxin synthesis. Here we show that transcription of atxA does not appear to differ in cells grown in 5% CO2 compared with cells grown in air. Using a new efficient method for gene replacement in B. anthracis, we constructed an atxA-null mutant in which the atxA-coding sequence on pXO1 is replaced with an omega km-2 cassette. Transcription of all three toxin genes is decreased in the absence of atxA. The pag gene possesses two apparent transcription start sites, P1 and P2; only transcripts with 5' ends mapping to P1 are decreased in the atxA-null mutant. Deletion analysis of the pag promoter region indicates that the 111 bp region upstream of the P1 site is sufficient for atxA-mediated activation of this transcript. The cya and lef genes each have one apparent start site for transcription. Transcripts with 5' ends mapping to these sites are not detected in the atxA-null mutant. The atxA-null mutant is avirulent in mice. Moreover, the antibody response to all three toxin proteins is decreased significantly in atxA-null mutant-infected mice. These data suggest that the atxA gene product also regulates toxin gene expression during infection.
- Published
- 1995
14. Regulation of the Bacillus anthracis protective antigen gene: CO2 and a trans-acting element activate transcription from one of two promoters
- Author
-
Zhihao Dai, M. Kaufman-Yarbray, and Theresa M. Koehler
- Subjects
DNA, Bacterial ,Transcription, Genetic ,Mutant ,Bacterial Toxins ,Molecular Sequence Data ,Biology ,Microbiology ,Transcription (biology) ,Gene expression ,Amino Acid Sequence ,Cloning, Molecular ,Promoter Regions, Genetic ,Molecular Biology ,Gene ,DNA Primers ,Antigens, Bacterial ,Base Sequence ,Activator (genetics) ,Promoter ,Gene Expression Regulation, Bacterial ,Carbon Dioxide ,Molecular biology ,nervous system ,Regulatory sequence ,Bacillus anthracis ,Trans-acting ,Research Article - Abstract
The pag gene of Bacillus anthracis, located on plasmid pXO1 (185 kb), encodes protective antigen, a component of the anthrax lethal and edema toxins. Synthesis of protective antigen is enhanced during growth of the organism with elevated levels of CO2. The CO2 effect is at the level of transcription, and pXO1-encoded regulatory factors have been implicated in control of pag expression. We used a Tn917-LTV3 insertion mutant of B. anthracis in which the wild-type pag gene on pXO1 was replaced with a pag-lacZ transcriptional fusion to monitor pag promoter activity. Expression of the pag-lacZ fusion is induced five- to eightfold during growth in 5% CO2 compared with growth in air. Growth in 20% CO2 increases transcription up to 19-fold. By monitoring pag-lacZ expression in atmospheres with different O2 and CO2 concentrations, we demonstrated definitively that the CO2 effect is specific and not simply a result of increased anaerobiosis. The results of 5' end mapping of pag transcripts indicate multiple sites of transcript initiation. We have determined two major apparent start sites, designated P1 and P2, located at positions -58 and -26 relative to the translation initiation codon, respectively. Analysis of total RNA from late-log-phase cells shows comparable initiation from P1 and P2 in wild-type strains grown in aerobic conditions. However, initiation from P1 is increased approximately 10-fold in cultures grown with an elevated level (5%) of CO2. We have identified a locus on pXO1, more than 13 kb upstream from the pag gene, which enhances pag transcription. When added in trans, this locus increases the level of transcripts with 5' ends mapping to P1 but has no effect on the level of transcripts with 5' ends mapping to P2. The CO2 effect on P1 is observed only in the presence of the activator locus.
- Published
- 1994
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.