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The ability to delay gratification is crucial for a successful and healthy life. An effective way for young children to learn this ability is to observe the action of adult models. However, the underlying neurocomputational mechanism remains unknown. Here, we tested the hypotheses that children employed either the simple imitation strategy or the goal-inference strategy when learning from adult models in a high-uncertainty context. Results of computational modeling indicated that children used the goal-inference strategy regardless of whether the adult model was their mother or a stranger. At the neural level, results showed that successful learning of delayed gratification was associated with enhanced interpersonal neural synchronization (INS) between children and the adult models in the dorsal lateral prefrontal cortex but was not associated with children’s own single-brain activity. Moreover, the discounting of future reward’s value obtained from computational modeling of the goal-inference strategy was positively correlated with the strength of INS. These findings from our exploratory study suggest that, even for 3-year-olds, the goal-inference strategy is used to learn delayed gratification from adult models, and the learning strategy is associated with neural interaction between the brains of children and adult models.

Kidney renal clear cell carcinoma (KIRC) is one of the most common primary malignancies of the urinary tract, highly heterogeneous, and increasing in incidence worldwide. Anoikis is a specific type of programmed cell death in which solid tumor cells or normal epithelial cells that do not have metastatic properties lose adhesion to the extracellular matrix (ECM) or undergo inappropriate cell adhesion-induced apoptosis. Anoikis is thought to play a critical role in tumorigenesis, maintenance, and treatment, according to an increasing amount of research. However, there is still some uncertainty regarding the general impact of anoikis-related genes (ARGs) on the prognostic importance, tumor microenvironment (TME) characteristics, and treatment reaction of KIRC patients. For this study, we used The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets to access the RNA sequencing results and clinical information from KIRC patients. 29 ARGs related to survival were found using differential analysis and univariate Cox regression analysis. The samples were then divided into two clusters that had different immune traits via unsupervised cluster analysis using 29 prognosis-associated differently expressed ARGs. Then, to build an ARGs signature, 7 genes (PLAU, EDA2R, AFP, PLG, TUBB3, APOBEC3G, and MALAT1) were found using Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis. The new ARGs signature demonstrated outstanding prognostic capability for KIRC patients' overall survival (OS). On the basis of the clinical parameters (gender, tumor grade, age, and stage) and the ARGs risk score, a clinical nomogram was created. This nomogram demonstrated excellent predictive value. In addition, patients that were categorized based on risk ratings also exhibited distinguishing immunological traits and medication sensitivity. In conclusion, for KIRC patients, we created an ARGs signature that strongly connects to immunological traits and therapy response. Clinicians may find this ARGs signature helpful in developing more individualized and detailed treatment strategies for KIRC patients.

Aims: The present study assessed cross-cultural differences in temperament and temperament stability between children from the United States (US) and the People’s Republic of China (PRC). Goals of the study include examining differences in three temperament factors (surgency, negative affectivity, and regulation/effortful control), conducting comparisons on fine-grained dimensions of factors demonstrating significant cross-cultural differences, and comparing temperament stability from infancy to toddlerhood. Methodology: The US sample (N = 147) and PRC sample (N = 128) consisted of children whose temperament was longitudinally assessed in infancy and toddlerhood using the Infant Behavior Questionnaire-Revised Short Form (IBQ-R SF) and the Early Childhood Behavior Questionnaire Short Form (ECBQ SF). Primary analyses involved evaluating mean differences in the three temperament factors: surgency, negative affectivity, and regulation/effortful control, with additional statistical tests conducted to investigate fine-grained distinctions. Results: Findings revealed main effects of culture for each factor with culture x time interactions indicating negative affectivity significantly differed in toddlerhood, t(273) = -8.27, P < .001, d = 1.00, 98.75% CI [-0.70, -0.37], and regulation in infancy, t(273) = -5.17, P < .001, d = 0.62, 98.75% CI [-0.62, -0.22]. Specifically, the US sample exhibited higher surgency at both time points, lower negative affectivity in toddlerhood, and lower regulation in infancy. In addition, little difference was noted in temperament stability between the US and Chinese samples. Conclusion: Our findings support previous reports identifying cultural differences in temperament and highlight that differences are not constant across early childhood, but rather that as development unfolds, their nature is subject to change.

La teoría y la investigación sobre el apego se utilizan en muchos contextos aplicados, incluidos los juzgados de familia, pero hay malentendidos muy extendidos y que a veces dan lugar a aplicaciones erróneas. El objetivo de este documento de consenso es, por lo tanto, mejorar la comprensión, contrarrestar la desinformación y dirigir el uso de la teoría del apego por parte de los juzgados de familia en la dirección de un apoyo basado en la evidencia, especialmente en lo que respecta a la protección de niñas y niños y las decisiones sobre su custodia. El artículo se divide en dos partes. En la primera abordamos los problemas relacionados con el uso de la teoría y la investigación sobre apego en los juzgados de familia, analizando las causas de tales problemas. Para ello examinamos las aplicaciones de la teoría del apego en los juzgados de familia en el marco del concepto de interés superior de niños y niñas, analizamos los malentendidos relacionados con la teoría del apego e identificamos los factores que han obstaculizado su adecuada aplicación. En la segunda parte ofrecemos recomendaciones para la aplicación de la teoría y la investigación sobre apego. Para ello establecemos tres principios del apego: la necesidad de niñas y niños de cuidadoras y cuidadores habituales que no maltraten, el valor de la continuidad de cuidados de calidad suficientemente buena y los beneficios de las redes de relaciones de apego. También analizamos la idoneidad de las evaluaciones de los estilos de apego y del comportamiento de los cuidadores para fundamentar la toma de decisiones de los juzgados de familia. Llegamos a la conclusión de que las evaluaciones de quienes cuidan deberían ocupar un lugar central. Aunque no hay consenso entre nosotros sobre el uso de las evaluaciones del estilo de apego para fundamentar las decisiones sobre custodia y protección infantil, dichas evaluaciones son actualmente las más adecuadas para orientar y dirigir las intervenciones de apoyo. Por último, proporcionamos orientaciones para guiar la futura colaboración en la investigación interdisciplinaria.

Liposcelis entomophila is a nuisance pest that seriously threatens the safety of stored grains owing in part of pesticide resistance. To facilitate better control of L. entomophila, future studies need to focus on gene function characterization. This requires the identification of suitable reference genes (RGs) for reverse-transcription quantitative PCR (RT-qPCR), which is crucial for the normalization of target gene expression. Here, we evaluated the expression stability of 12 candidate RGs in L. entomophila across six experimental conditions (developmental stage, population, body part, phosphine fumigation, hypoxia induction, and temperature treatment). Under developmental conditions, ACTB, HSP70, and RPL23 were the most stable RGs. Among the different populations and body parts, RPL23, EF1a, and ACTB were the most stable RGs. Comparison of gene expression among adults under different environmental conditions revealed that at least seven RGs were fit for normalizing gene expression data. Among them, RPL23, EF1a, and ACTB showed the best fit as RGs in RT-qPCR analysis. After validation, our results demonstrated that EF1a and ACTB, as well as their combination, were the ideal RGs for L. entomophila across various experimental conditions. This study provides a comprehensive RG selection suggestion for gene expression studies of L. entomophila and will facilitate future functional genomic research on the development and tolerance of environmental factors in psocids.

To reveal population divergence in the mate choice and its mechanistic basis, the mating rhythm, mating preference and male volatile profiles of Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae) were studied. During ten days after emergence, six geographical populations of T. castaneum exhibited an identical daily or circadian mating rhythm. Males showed significant attraction to females while females did not show attraction to males. Furthermore, male attraction to females varied significantly within six populations, while female preference to males did not. Males from the HBWH population were most attractive to females sequentially followed by males from the populations of SHJD, HNZZ, GA1, GDST, and ZJJX, which was positively correlated with their 4,8-dimethyldecanal (DMD) production. Gnotobiotic reinoculation of two gut bacteria, Citrobacter freundii and Escherichia coli, to axenic males significantly enhanced their attraction to females and DMD production. It could be concluded that: (i) in the mate choice of T. castaneum, males were more like the sexual pheromone senders and females were the pheromone receivers; (ii) there was detectable divergence in the chemical communication among six populations of T. castaneum, which could be mainly attributed to alterations in the male pheromone production rather than changes in the female pheromone perception; and (iii) gut bacteria could modulate the chemical communication of the host by interfering with the pheromone production. Such knowledge will provide insights into optimizing trapping technologies for pest management.

Although noble metal nanocrystals have been studied extensively in the past decades, the shape-controlled synthesis of non-noble metal nanocrystals has remained challenging with limited success, which is a grand obstacle to their wide applications. Herein, a novel lattice mismatch-involved shape-control mechanism of Cu nanocrystals in a seed-mediated synthesis is reported, which can produce Cu nanoplates in high yield with tailored sizes (28-130 nm), holding great potential in optical and catalytic applications. The lattice mismatch between Cu and the seed is found effective in inducing crystallographic defects for symmetry breaking toward anisotropic nanocrystals. While a too-large lattice mismatch (11.7% for Au seeds) leads to multiple twin defects to form quasi-spherical Cu nanocrystals, an appropriately large lattice mismatch (7.7% for Pt and 6.9% for Pd seeds) successfully induces planar defects for the formation of Cu nanoplates. The size of the Cu nanoplates is customizable by controlling the concentration of the seeds, leading to tunable optical properties. A prototype of a colorimetric indicator with Cu nanoplates, potentially applicable to the safety control of foods and drugs is demonstrated. This mechanism paves a new way for the shape-controlled synthesis of Cu and other metal nanocrystals for a broad range of applications.

To reveal population divergence in the mate choice and its mechanistic basis, the mating rhythm, mating preference and male volatile profiles of Tribolium castaneum were studied. During ten days after emergence, six geographical populations of T. castaneum exhibited an identical daily or diurnal mating rhythm. Males showed significant attraction to females while females did not show attraction to males. Furthermore, male attraction to females varied significantly within six populations, while female preference to males did not. Males from the HBWH population were most attractive to females sequentially followed by males from the populations of SHJD, HNZZ, GA1, GDST, and ZJJX, which was positively correlated with their 4,8-dimethyldecanal (DMD) production. Gnotobiotic reinoculation of two gut bacteria, Citrobacter freundii and Escherichia coli, to axenic males significantly enhanced their attraction to females and DMD production. It could be concluded that: (i) in the mate choice of T. castaneum, males were more like the sexual pheromone senders and females were the pheromone receivers; (ii) there was detectable divergence in the chemical communication among six populations of T. castaneum, which could be attributed to alterations in the male pheromone production rather than changes in the female pheromone perception; and (iii) gut bacteria could modulate the chemical communication of the host by interfering with the pheromone production. Such knowledge will provide insights into optimizing trapping technologies for pest management.

We examined hypothalamic–pituitary–adrenal stress reactivity as an indicator of differential susceptibility in moderating the links between family socioeconomic status (SES) and executive function (EF) in three-year-old urban Chinese children. The participants included 77 children (36 girls) and their families. Family income, parents’ education, and level of occupation were reported by the mothers and aggregated into a composite index of family SES. We assessed the children’s biological reactivity by salivary cortisol responses to a mild stressor, which we calculated by the area under the curve with respect to increase. We measured EF skills with a series of laboratory tasks. The results revealed significant interactive effects of SES and cortisol reactivity on children’s cognitive inhibitory control. More specifically, SES was significantly related to cognitive inhibitory control for children with higher cortisol reactivity. Conversely, for children with lower cortisol reactivity, there were no significant correlations between SES and cognitive inhibitory control. Region of significance analyses indicated a moderating effect supporting the “bright side” of differential susceptibility, meaning that high cortisol reactive children were more likely to benefit from high levels of SES. The results also manifested a direct association between SES and children’s working memory, and this association was not dependent on the features of cortisol reactivity. SES did not relate to hot EF. The results highlight the SES disparity in different EF elements and may help in the development of targeted interventions.

This paper defined heavily polluting enterprises as the treatment group based on relevant data on the listed companies in the 5 years from 2016 to 2020. Companies other than the heavily polluting enterprises were defined as the control group. An empirical analysis applying the double-difference technique was performed to assess the impact of China’s environmental tax on the economic performance and technological innovation input of industries that pollute heavily. The findings demonstrated an increased positive effects of environmental taxes on the economic performance and technology innovation input of heavily polluting industries since the inception of the environmental tax in China. As some enterprises in heavily polluting industries are under increased financial pressure owing to green upgrades and the low environmental tax rate in some regions, recommendations are presented to improve the environmental regulation system, adapt environmental taxes to local conditions, and strengthen the implementation and supervision of environmental taxes through big data technology.

Objective: The aim of the research was to study the effect of azithromycin (AZM) in the treatment of MDR P. aeruginosa VAP combined with other antimicrobial therapies.Methods: The clinical outcomes were retrospectively collected and analyzed to elucidate the efficacy of different combinations involving azithromycin in the treatment of MDR-PA VAP. The minimal inhibitory concentration (MIC) of five drugs was measured by the agar dilution method against 27 isolates of MDR-PA, alone or in combination.Results: The incidence of VAP has increased approximately to 10.4% (961/9245) in 5 years and 18.4% (177/961) caused by P. aeruginosa ranking fourth. A total of 151 cases of MDR P. aeruginosa were included in the clinical retrospective study. Clinical efficacy results are as follows: meropenem + azithromycin (MEM + AZM) was 69.2% (9/13), cefoperazone/sulbactam + azithromycin (SCF + AZM) was 60% (6/10), and the combination of three drugs containing AZM was 69.2% (9/13). The curative effect of meropenem + amikacin (MEM + AMK) was better than that of the meropenem + levofloxacin (MEM + LEV) group, p = 0.029 (p < 0.05). The curative effect of cefoperazone/sulbactam + amikacin (SCF + AMK) was better than that of the cefoperazone/sulbactam + levofloxacin (SCF + LEV) group, p = 0.025 (p < 0.05). There was no significant difference between combinations of two or three drugs containing AZM, p > 0.05 (p = 0.806). From the MIC results, the AMK single drug was already very sensitive to the selected strains. When MEM or SCF was combined with AZM, the sensitivity of them to strains can be significantly increased. When combined with MEM and AZM, the MIC50 and MIC90 of MEM decreased to 1 and 2 ug/mL from 8 to 32 ug/mL. When combined with SCF + AZM, the MIC50 of SCF decreased to 16 ug/mL, and the curve shifted obviously. However, for the combination of SCF + LEV + AZM, MIC50 and MIC90 could not achieve substantive changes. From the FIC index results, the main actions of MEM + AZM were additive effects, accounting for 72%; for the combination of SCF + AZM, the additive effect was 40%. The combination of AMK or LEV with AZM mainly showed unrelated effects, and the combination of three drugs could not improve the positive correlation between LEV and AZM.Conclusion: AZM may increase the effect of MEM or SCF against MDR P. aeruginosa VAP. Based on MEM or SCF combined with AMK or AZM, we can achieve a good effect in the treatment of MDR P. aeruginosa VAP.

ObjectivesThe present study examined parental sleep-supporting practices during toddlerhood in relation to temperament across 14 cultures. We hypothesized that passive sleep-supporting techniques (e.g., talking, cuddling), but not active techniques (e.g., walking, doing an activity together), would be associated with less challenging temperament profiles: higher Surgency (SUR) and Effortful Control (EC) and lower Negative Emotionality (NE), with fine-grained dimensions exhibiting relationships consistent with their overarching factors (e.g., parallel passive sleep-supporting approach effects for dimensions of NE).MethodsCaregivers (N = 841) across 14 cultures (M = 61 families per site) reported toddler (between 17 and 40 months of age; 52% male) temperament and sleep-supporting activities. Utilizing linear multilevel regression models and group-mean centering procedures, we assessed the role of between- and within-cultural variance in sleep-supporting practices in relation to temperament.ResultsBoth within-and between-culture differences in passive sleep-supporting techniques were associated with temperament attributes, (e.g., lower NE at the between-culture level; higher within-culture EC). For active techniques only within-culture effects were significant (e.g., demonstrating a positive association with NE). Adding sleep-supporting behaviors to the regression models accounted for significantly more between-culture temperament variance than child age and gender alone.ConclusionHypotheses were largely supported. Findings suggest parental sleep practices could be potential targets for interventions to mitigate risk posed by challenging temperament profiles (e.g., reducing active techniques that are associated with greater distress proneness and NE).

The development of children’s early motor can be regarded as an individual’s early explicit intelligence, and the lifelong development of motor is closely related to an individual’s cognitive development, and personality and social development. The revolutionary development of cognitive neuroscience and brain science technology provides the foundation and opportunity for studying neural mechanisms of lifelong development in the field of motor. From the perspective of lifelong development, the generation of motor and the acquisition of motor skills are milestones of infant development. The effectiveness, accuracy and stability of motor skills in childhood and adolescence develop rapidly. From the early to late adulthood, motor ability experiences a peak and then a significant decline. Current research on neural mechanisms of actions mainly discusses the changes in brain structure and brain network connections behind the generation, development and decline of actions during the lifelong development. From the perspective of cognitive science and cognitive neuroscience, this article reviewed the developmental characteristics of individual motor in infancy, child, adolescence and adulthood and neural mechanisms behind it. It also put forward the challenges and problems to be solved in the future of cognitive neuroscience, and analyzed and predicted the future research and development in this field in China.

Summary Background Several orthognathic procedures have been applied to correct skeletal anterior open bites (SAOB). Which method is most stable has been debated and no consensus has been reached and there is no conclusive evidence for clinicians to use. Objective To analyse whether maxillary, mandibular, or bimaxillary surgery provides a better stability. Materials and methods A systematic search was conducted up to December 2020 using PubMed, EMBASE, Medline, Scopus, Web of Science, Cochrane CENTRAL, and Google Scholar. We made direct comparisons among the controlled trials and also made indirect comparisons via subgroup analysis on the aspects of occlusional, skeletal, and dento-alveolar stability to assess the overall stability of each method. Results Finally 16 cohort studies were identified. At the occlusional level, pooled change in overbite was 0.21 mm in maxillary surgery, 0.37 mm in bimaxillary surgery, and −0.32 mm in mandibular surgery. At the skeletal level, pooled sella–nasion–Point A angle (SNA) was −0.12 degrees in bimaxillary surgery, −0.37 degrees in maxillary surgery and −0.20 degrees in mandibular surgery. The sella–nasion to palatal plane angle (SNPP) relapsed to a statistically significant degree in all samples received single maxillary surgery. Relapse of the sella–nasion–Point B angle (SNB) was 0.47 degrees in mandibular setback, −1.8 degrees in mandibular advancement, and −0.48 degrees in maxillary surgery. The Sella–Nasion to mandibular plane angle (SNMP) relapsed more in procedures involving bilateral sagittal split osteotomy than in other procedures. As for dento-alveolar changes, intrusion of molars and extrusion of incisors took place in most patients. Conclusions Bimaxillary surgery produced the most beneficial post-operative increase in overbite, maxillary surgery led to a lesser but still positive overbite change, and mandibular surgery correlated with some extent of relapse. Skeletally, bimaxillary surgery was more stable than maxillary surgery at both SNA and SNPP; SNB was more stable in mandibular setback than advancement; and SNMP was unstable in both mandibular and bimaxillary surgeries versus maxillary surgery with comparable surgical changes. Dento-alveolar compensation helped maintain a positive overbite. Registration number CRD42020198088.

Background The strategy of anesthesia used during ablation of atrial fibrillation (AF) remains controversial. This study aimed to compare sedation with general anesthesia (GA) for catheter ablation of AF. Hypothesis The presence of AF is associated with an increased risk of stroke and heart failure and decreased quality of life and survival. Methods We carried out a retrospective single-centered study with 351 patients undergoing the first ablation procedure for AF under sedation or GA. The main outcome was freedom from recurrence of AF at 1 year. The total time of staying at the ablation laboratory and procedure cost were also calculated. Results Freedom from atrial arrhythmia and ablation time did not differ between AF patients under sedation and GA (77.9% vs 79.9% and 42.27 ± 9.84 minutes vs 41.51 ± 9.27 minutes, respectively), while the total procedure time and cost were lower in patients who underwent sedation than GA (171.39 ± 45.09 minutes vs 202.92 ± 43.85 and 8.00 ± 7.02 CNY vs 8.79 ± 11.63 CNY, respectively). Conclusion GA is not superior to sedation, in terms of ablation time and freedom from atrial arrhythmia at 1 year, whereas patients with GA had more anesthesia time and procedure cost than sedation.

This research aimed to examine the relations among harsh discipline (including psychological aggression and corporal punishment), parent-child attachment, and peer attachment. More specifically, two mediation models (one for psychological aggression, one for corporal punishment) were investigated in which father-child attachment and mother-child attachment were hypothesized to mediate the relations between both paternal and maternal harsh discipline and peer attachment. In addition, differences across gender of the mediation model were examined. Participants were 668 children in grades four to eight and both their parents in China. The Chinese version of Parent-Child Conflict Tactics Scale (CTSPC) and the Inventory of Parent and Peer Attachment (IPPA) were used as the main assessment tools to measure parental use of harsh discipline, parent-child attachment, and peer attachment. Findings revealed that the direct path from neither fathers’ nor mothers’ harsh discipline to peer attachment was significant. Harsh discipline by one parent was indirectly related to peer attachment through the attachment between this parent and the child, but not through the attachment between his/her spouse and the child. In addition, the direct and indirect relations between harsh parental discipline and peer attachment did not differ across child gender. The findings provided an important supplement and extension to previous examinations of the factors associated with peer attachment and its mechanisms. In addition, the results also suggested the need for intervention programs aiming at improving children’s peer relationships to take the parenting and parent-child relationships into account.

Many metal organic frameworks (MOFs) incorporate metal oxide clusters as nodes. Node sites where linkers are missing can be catalytic sites. We now show how to dial in the number and occupancy of such sites in MIL-53 and MIL-68, which incorporate aluminum-oxide-like nodes. The methods involve modulators used in synthesis and postsynthesis reactions to control the modulator-derived groups on these sites. We illustrate the methods using formic acid as a modulator, giving formate ligands on the sites, and these can be removed to leave μ2-OH groups and open Lewis acid sites. Methanol dehydration was used as a catalytic reaction to probe these sites, with infrared spectra giving evidence of methoxide ligands as reaction intermediates. Control of node surface chemistry opens the door for placement of a variety of ligands on a wide range of metal oxide cluster nodes for dialing in reactivity and catalytic properties of a potentially immense class of structurally well-defined materials.

Jinlin Li,1 Qi Wang,1 Qingping Zhang,2 Zhengyan Wang,1 Xin Wan,3 Chenfang Miao,4 Xi Zeng5 1Department of Respiratory Medicine, Suizhou Hospital, Hubei University of Medicine, Suizhou, Hubei 441300, People’s Republic of China; 2Department of Otolaryngology Head and Neck Surgery, Suizhou Hospital, Hubei University of Medicine, Suizhou, Hubei 441300, People’s Republic of China; 3Department of Critical Care Medicine, Mindong Hospital of Fujian Medical University, Fuan, Fujian 355000, People’s Republic of China; 4Department of Anesthesiology, Mindong Hospital of Fujian Medical University, Fuan, Fujian 355000, People’s Republic of China; 5Department of Geriatrics, Mindong Hospital of Fujian Medical University, Fuan, Fujian 355000, People’s Republic of ChinaCorrespondence: Xi ZengDepartment of Geriatrics, Mindong Hospital of Fujian Medical University, No. 89 Heshan Road, Fuan City 355000, Fujian Province, People’s Republic of ChinaTel +86 18596658975Email 987295461@qq.comBackground: Vascular cell adhesion molecule-1 (VCAM-1) is associated with vascular-related inflammation and atherosclerosis. This study aimed to evaluate whether VCAM-1 can be used for an indication of increased risk of CV events in patients with COPD.Methods: Serum VCAM-1 levels were measured in 163 COPD patients. All COPD patients were prospectively followed up for a median period of 48 months (range=3– 54). Cox proportional hazard analysis was performed to evaluate the prognostic value of serum VCAM-1 for predicting CV events.Results: Serum VCAM-1 levels were higher in COPD patients with CV events than in those without CV events (1174.4± 365.3 ng/mL vs 947.8± 293.2 ng/mL; P< 0.001). The logistic regression analysis revealed that serum VCAM-1 (OR=1.750; 95% CI, 1.324– 2.428; Ptrend= 0.0012) was independently associated with CVD (cardiovascular disease) history after adjusting for age, sex, BMI, current smoker, current drinker, admission systolic and diastolic BP, LVEF and laboratory measurements in patients with COPD at baseline. The Kaplan–Meier analysis demonstrated that the rate of CV events was higher in COPD patients with serum VCAM-1 levels above the median (517.3 ng/mL) than in those with VCAM-1 levels below the median. The Cox proportional hazard analysis revealed that serum VCAM-1 (HR=2.617; 95% CI, 1.673– 5.328; Ptrend< 0.001) may be an independent prognostic factor for CV events in the COPD patients.Conclusion: Our results suggested that serum VCAM-1 was significantly and independently associated with CV events in COPD patients. The inflammatory marker may help clinicians predict CV complications early.Keywords: vascular cell adhesion molecule-1, chronic obstructive pulmonary disease, cardiovascular events, prognostic value

The decision-making of cash holdings is very important for the daily operation of enterprises. This present paper tries to establish uncertain optimal cash holding models with the constraint of saf...

Objective. To examine the clinical efficacy of budesonide/glycopyrronium bromide/formoterol (Breztri Aerosphere) as an adjunct to acute respiratory distress syndrome (ARDS). Methods. A prospective study enrolled 120 patients with pulmonary endogenous ARDS admitted to the Department of Critical Care Medicine at the Fourth Hospital of Baotou from January 2017 to January 2020, and all enrollments were assigned (1 : 1) to receive conventional treatment (control group) or Breztri Aerosphere (study group). Results. Breztri Aerosphere was associated with a significantly higher total efficacy versus conventional treatment. Breztri Aerosphere resulted in significantly lower acute physiology and chronic health evaluation scoring system (APACHE II) scores and Murray lung injury scores versus conventional treatment. Both groups saw an increase in the partial pressure of carbon dioxide (PCO2), partial pressure of oxygen (PO2), and oxygen saturation (SaO2) after treatment, with higher levels seen in patients given Breztri Aerosphere. After treatment, systemic vascular resistance (SVR) in both groups rose markedly, with greater elevation witnessed in the study group. The patients given Breztri Aerosphere showed significantly lower levels of pulmonary vascular resistance (PVR), mean pulmonary arterial pressure (MAPA), pulmonary artery wedge pressure (PAWP), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), and procalcitonin (PCT) versus those receiving conventional treatment. The patients experienced shorter mechanical ventilation time and intensive care unit (ICU) time after treatment of Breztri Aerosphere versus conventional treatment. Conclusion. Adjuvant therapy with Breztri Aerosphere in ARDS can significantly lower APACHE II scores and Murray lung injury scores, improve blood gas indexes and pulmonary circulation function indexes, and shorten mechanical ventilation time and ICU time, which may be attributed to its improvement of organism inflammation status and reduction of inflammatory factors.

Attachment theory and research are drawn upon in many applied settings, including family courts, but misunderstandings are widespread and sometimes result in misapplications. The aim of this consensus statement is, therefore, to enhance understanding, counter misinformation, and steer family-court utilisation of attachment theory in a supportive, evidence-based direction, especially with regard to child protection and child custody decision-making. The article is divided into two parts. In the first, we address problems related to the use of attachment theory and research in family courts, and discuss reasons for these problems. To this end, we examine family court applications of attachment theory in the current context of the best-interest-of-the-child standard, discuss misunderstandings regarding attachment theory, and identify factors that have hindered accurate implementation. In the second part, we provide recommendations for the application of attachment theory and research. To this end, we set out three attachment principles: the child’s need for familiar, non-abusive caregivers; the value of continuity of good-enough care; and the benefits of networks of attachment relationships. We also discuss the suitability of assessments of attachment quality and caregiving behaviour to inform family court decision-making. We conclude that assessments of caregiver behaviour should take center stage. Although there is dissensus among us regarding the use of assessments o attachment quality to inform child custody and child-protection decisions, such assessments are currently most suitable for targeting and directing supportive interventions. Finally, we provide directions to guide future interdisciplinary research collaboration. info:eu-repo/semantics/publishedVersion

Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples. In this paper, we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product insects. Subsequently, the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan® probe real-time quantitative PCR, and host reproductive fitness parameters were compared. T. confusum was the only Wolbachia infected species in all the surveyed species. Wolbachia infection density consistently increased with the development of T. confusum and plateaued at 3.7 × 107 wsp copies per individual insect at the young adult stage. Wolbachia densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and organs. Uninfected female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected males. Embryogenesis and egg hatch rate were specifically inhibited after Wolbachia elimination, while other traits, including the number of eggs, pupation rate and sex ratio, remained unaffected by tetracycline treatment. Our results show that the TaqMan® probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time qPCR. The fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle. Keywords: Wolbachia, Tenebrionidae, Tribolium spp., Stored product insects, Endosymbiont, Infection density, Tetracycline

Maize is an important crop in the world, nitrogen stress severely reduces maize yield. Although a large number of studies have identified the expression changes of microRNAs (miRNAs) under N stress in several species, the miRNAs expression patterns of N-deficient plants under N resupply remain unclear. The primary objective of this study was to identify miRNAs in response to nitrogen stress and understand relevant physiological changes in nitrogen-deficient maize after nitrogen resupply. Physiological parameters were measured to study relevant physiological changes under different nitrogen conditions. Small RNA sequencing and qRT-PCR analysis were performed to understand the response of miRNAs under different nitrogen conditions. The content of chlorophyll, soluble protein and nitrate nitrogen decreased than CK by 0.52, 0.49 and 0.82 times after N deficiency treatment and increased than ND by 0.52, 1.36 and 0.65 times after N resupply, respectively. Conversely, the activity of superoxide dismutase (SOD) and peroxidase (POD) increased by 0.67 and 1.64 times than CK after N deficiency, respectively, and decreased by 0.09 and 0.35 times than ND after N resupply. A total of 226 known miRNAs were identified by sRNA sequencing; 106 miRNAs were differentially expressed between the control and N-deficient groups, and 103 were differentially expressed between the N-deficient and N-resupply groups (P

The goal of our study was to determine if phosphine exposure changed the fecundity of the surviving Liposcelis entomophila, and the fitness of resulting offspring. Two strains of L. entomop...

Studies have explored brain structural abnormalities in patients with primary insomnia (PI). However, most of them are based on volumetric measures, in a specific region of interest, and have small sample sizes. Here, we investigated changes in cortical morphology (thickness and volume) in PI using an advanced surface-based morphometric method. Sixty-seven patients with PI and 55 matched healthy controls were recruited for this study and underwent a structural magnetic resonance imaging scan. The reconstructed cortical surface was processed by Freesurfer 6.0. A general linear model was used to explore group differences in surface-based morphometric features. Furthermore, the association between these cortical features and clinical characteristics were assessed in the PI group. Compared to controls, PI patients showed cortical thickening in the left orbital frontal cortex (OFC), right rostral anterior cingulate cortex (rACC), left middle cingulate cortex (MCC), bilateral insula, left superior parietal lobule (SPL), and right fusiform area (FFA), and showed increased cortical volume in the left OFC, right rACC, bilateral rostral middle frontal gyrus, and right FFA. Cortical thickness in the right OFC and FFA was positively correlated with the severity of insomnia in the PI group, suggesting a right-lateralized relationship. This study was the first to explore multiple-scale cortical morphometric changes in a relatively large sample of PI patients. Our results suggest that hypertrophic cortical morphology may underlie the neuropathology of primary insomnia.

The present study examined bidirectional effects between sleep problems (nocturnal awakenings and insufficient nocturnal sleep) and infant development (gross motor, fine motor, and cognition) in a sample of 182 infants (89 girls) and their parents living in Beijing (China). Using 3 waves of longitudinal data (at 6 months, 1 year, and 2 years of age), this study (a) explored the differences in sleep patterns and developmental outcomes between infants in the current sample and infants from other cultures; and primarily examined (b) whether nocturnal awakenings and insufficient nocturnal sleep prospectively predicted infant development; (c) or whether infant development predicted sleep problems. Mothers reported their children's sleep problems, and infant development was assessed with Bayley III. Sleep patterns of Beijing infants were slightly different from those from Finland and Singapore, and most scores on Bayley III in this Beijing sample were higher than those in Danish, Dutch and Sri Lankan samples. Sleep problems and developmental measures were stable across the 3 times of assessments, but cross-lagged associations were limited in number and strength. High scores on the Bayley at 6 months predicted less nocturnal awakenings at 1 year of age. Insufficient nocturnal sleep at 1 year predicted poor fine motor development at 2 years. Thus, findings suggest some bidirectional associations between infant development and sleep problems and further highlight the need to understand these relations within specific cultural contexts.

While social interaction between a mother and her child has been found to play an important role in the child’s committed compliance, the underlying neurocognitive process remains unclear. To investigate this process, we simultaneously recorded and assessed brain activity in 7-year-old children and in children’s mothers or strangers during a free-play task using functional near-infrared spectroscopy-based hyperscanning. The results showed that a child’s committed compliance was positively associated with the child’s responsiveness but was negatively associated with mutual responsiveness and was not associated with the mother’s responsiveness during mother–child interactions. Moreover, interpersonal neural synchronization (INS) at the temporoparietal junction mediated the relationship between the child’s responsiveness and the child’s committed compliance during mother–child interactions when the child’s brain activity lagged behind that of the mother. However, these effects were not found during stranger–child interactions, nor were there significant effects in the mother–child pair when no real interactions occurred. Finally, we found a transfer effect of a child’s committed compliance from mother–child interactions to stranger–child interactions via the mediation of mother–child INS, but the opposite did not occur. Together, these findings suggest that a child’s responsiveness during mother–child interactions can significantly facilitate her or his committed compliance by increasing mother–child INS.

Background: Catheter ablation of atrial fibrillation is an alternative treatment for patients with tachycardia-bradycardia syndrome (TBS) to avoid pacemaker implantation. The risk stratification for atrial fibrillation and outcomes between ablation and pacing has not been fully evaluated.Methods: This retrospective study involved 306 TBS patients, including 141 patients who received catheter ablation (Ablation group, age: 62.2 ± 9.0 months, mean longest pauses: 5.2 ± 2.2 s) and 165 patients who received pacemaker implement (Pacing group, age: 62.3 ± 9.1 months, mean longest pauses: 6.0 ± 2.3 s). The primary endpoint was a composite of call cause mortality, cardiovascular-related hospitalization or thrombosis events (stroke, or peripheral thrombosis). The second endpoint was progress of atrial fibrillation and heart failure.Results: After a median follow-up of 75.4 months, the primary endpoint occurred in significantly higher patients in the pacing group than in the ablation group (59.4 vs.15.6%, OR 6.05, 95% CI: 3.73–9.80, P < 0.001). None of deaths was occurred in ablation group, and 1 death occurred due to cancer. Cardiovascular-related hospitalization occurred in 50.9% of the pacing group compared with 14.2% in the ablation group (OR: 4.87, 95% CI: 2.99–7.95, P < 0.001). More thrombosis events occurred in the pacing group than in the ablation group (12.7 vs. 2.1%, OR 6.06, 95% CI: 1.81–20.35, P = 0.004). Significant more patients progressed to persistent atrial fibrillation in pacing group than in ablation group (23.6 vs. 2.1%, P < 0.001). The NYHA classification of the pacing group was significantly higher than that of the ablation group (2.11 ± 0.83 vs. 1.50 ± 0.74, P < 0.001). The proportion of antiarrhythmic drugs and anticoagulants used in the pacing group was significantly higher than that in the ablation group (41.2 vs. 7.1%, P < 0.001; 16.4 vs. 2.1%, P = 0.009).Conclusion: Catheter ablation for patients with TBS was associated with a significantly lower rate of a composite end point of cardiovascular related hospitalization and thromboembolic events. Furthermore, catheter ablation reduced the progression of atrial fibrillation and heart failure.

Sentiment analysis based on statistics has rapidly developed in deep-learning. Bilateral attention neural network (BANN), especially Bidirectional Encoder Representations from Transformers (BERT), has reached high accuracy. However, with the increase of network depth and large-scale corpus, the computational overhead of BANN increases geometrically. How to reduce training corpus scale has correspondingly become an important research focus. This paper proposes a reduced corpus scale method called Concept-BERT, which consists of the following steps: firstly, using Formal Concept Analysis (FCA), Concept-BERT mines the association rules among corpus and reduces corpus attributes, and hence reducing corpus scale; secondly, reduced-corpus is inputed to BERT and the result is obtained; finally, the attention of Concept-BERT is analyzed. Concept-BERT is experimented for sentiment analysis on CoLA, SST-2, Dianping and Blogsenti, and its accuracy reaches 81.1, 92.9, 77.9 and 86.7 respectively. Our experimental results show that the proposed method has the same accuracy as BERT, using low-scale corpus and low overhead, and low-scale corpus doesn't affect model attention.

Although the research interest in parenting behaviors of Chinese parents has increased during the past two decades, there remains a lack of an adequate and comprehensive Chinese assessment tool for these parenting behaviors in early childhood. Drawn from two samples of Chinese mothers with young children (i.e., 1- to 4-year-olds), this research addressed this gap by examining the factor structure, reliability, and validity of a Chinese version of the Comprehensive Early Childhood Parenting Questionnaire (CECPAQ-CV). Confirmatory factor analyses showed that a 5-factor model, consisting of 13 micro-dimensions of parenting behaviors, best fitted the data for Sample 1 (N1 = 2179) compared with 1-factor, 2-factor, and 4-factor models. This 5-factor model was further validated with the data for Sample 2 (N2 = 160). Reliability was good. The criterion validity of the CECPAQ-CV was supported by expected relations with maternal parenting stress and child externalizing and internalizing behaviors. The convergent and discriminant validity of the CECPAQ-CV was established with the Parent-Child Conflict Tactics Scale. The results indicate that the CECPAC-CV holds promise as a reliable and valid tool to measure important dimensions of early parenting in China.

This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment. Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX. The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45 μg DOX per mg of the carrier. The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV–vis spectroscopy. The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C. The DOX accumulative release could reach 78% under the pH of 5. A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment. The cytotoxicity study indicated that, under the DOX concentration of 4 μg ml−1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%). UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.

Item does not contain fulltext Television exposure in early childhood has increased, with concerns raised regarding adverse effects on social-emotional development, and emerging self-regulation in particular. The present study addressed television exposure (i.e., amount of time watching TV) and its associations with toddler behavioral/emotional dysregulation, examining potential differences across 14 cultures. The sample consisted of an average of 60 toddlers from each of the 14 countries from the Joint Effort Toddler Temperament Consortium (JETTC; Gartstein & Putnam, 2018). Analyses were conducted relying on the multi-level modeling framework (MLM), accounting for between- and within-culture variability, and examining the extent to which TV exposure contributions were universal vs. variable across sites. Effects of time watching TV were evaluated in relation to temperament reactivity and regulation, as well as measures of emotional reactivity, attention difficulties, and aggression. Results indicated that more time spent watching TV was associated with higher ratings on Negative Emotionality, emotional reactivity, aggression, and attention problems, as well as lower levels of soothability. However, links between TV exposure and both attention problems and soothability varied significantly between cultures. Taken together, results demonstrate that increased time spent watching television was generally associated with dysregulation, although effects were not consistently uniform, but rather varied as a function of culturally-dependent contextual factors. 15 p.

Supplemental material, sj-docx-1-tej-10.1177_2041731421995465 for Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering by Lufei Wang, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M Musicant, Kshitij Parag-Sharma, Xiangxiang Hu, Henry C Tseng, Jen-Tsan Chi, Zhengyan Wang, Antonio L Amelio and Ching-Chang Ko in Journal of Tissue Engineering

Supplemental material, sj-docx-1-tej-10.1177_2041731421995465 for Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering by Lufei Wang, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M Musicant, Kshitij Parag-Sharma, Xiangxiang Hu, Henry C Tseng, Jen-Tsan Chi, Zhengyan Wang, Antonio L Amelio and Ching-Chang Ko in Journal of Tissue Engineering

With the popularity of large-scale corpora, statistics-based models have become mainstream model in Natural Language Processing (NLP). The Bidirectional Encoder Representations from Transformers (BERT), as one of those models, has achieved excellent results in various tasks of NLP since its emergence. But it still has shortcomings, such as poor capability of extracting local features and exploding of training gradients. After analyzing the shortcomings of BERT, this paper proposed an Information-gain Association Vector Semi-supervised Bidirectional Encoder Representations from Transformers (IAS-BERT) model, which improves the capability of capturing local features. Considering the influence of feature's polarity to overall sentiment and association between two word-embeddings, we use information gain on the training corpus. And then, the information gain results are used as an annotation of training corpus to generate a new word embedding. At the same time, we use forward-matching to optimize the computational overhead of IAS-BERT. We experiment the model on dataset of sentiment analysis, and it have achieved good results.