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The seasonal dynamic of suprapermafrost groundwater significantly affects runoff generation and concentration in permafrost basins and is a leading issue that must urgently be addressed in hydrological research in cold and alpine regions. In this study, the seasonal dynamic process of the suprapermafrost groundwater level (SGL), vertical gradient changes of soil temperature (ST) and moisture content in the active layer (AL), and river level changes were systematically analyzed at four permafrost watersheds in the Qinghai–Tibet Plateau using comparative analysis and the nonlinear correlation evaluation method. How freeze–thaw processes impact seasonal SGL, and the links between SGL and surface runoff, were also discussed. The SGL process in a hydrological year can be divided into four periods: (A) a rapid falling period (October–middle November), (B) a stable low-water period (late November–May), (C) a rapid rising period (approximately June), and (D) a stable high-water period (July–September), which synchronously respond to seasonal variations in soil moisture and temperature in the AL. The characteristics and causes of SGL changes varied significantly during the four different periods. The freeze-thaw process of the AL has crucial regulatory effects on SGL and surface runoff in permafrost watersheds. During Period A, with rapid AL freezing, the ST had a dominant impact on the SGL. In Period B, the AL was entirely frozen because of the stably low ST, and the SGL dropped to the lowest level with small changes. During Period C, ST in the deep soil layers of the active layer (below 50 cm depth) significantly impacted the SGL (nonlinear correlation coefficient R2>0.74, P

Additional file 6: Supplemental Table S1. siRNA target sequences.

Additional file 7: Supplemental Table S2. Real-time PCR primers.

Mesenchymal stem cells (MSCs) repair tissue injury and may be used to treat immune associated diseases. In carbon tetrachloride (CCl4)-induced liver injury murine model, we administered MSCs. When MSCs were transmitted to young and old mice with liver injury, more MSCs were recruited in old mice. In old mice, inflammation, characterized by TNF-α and IL-6, was increased due to hyper-activation and hyper-function of Kupffer cells. Blocking Kupffer cells decreased MSCs migration in old mice. In vitro, Kupffer cells isolated from old mice secreted more inflammatory cytokines and chemokines. Thus, hyper-activation of Kupffer cells in old mice increased recruitment of MSCs after their therapeutic administration.