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Supplemental material, DS_10.1369_0022155419871550 for Elevated ATF4 Expression in Odontogenic Keratocysts Epithelia: Potential Involvement in Tissue Hypoxia and Stromal M2 Macrophage Infiltration by Wen-Qun Zhong, Zhi-Zheng Li, Hao Jiang, Yan-Ping Zou, Hai-Tao Wang, Yu Cai, Yi Zhao and Ji-Hong Zhao in Journal of Histochemistry & Cytochemistry

Progenies derived from rice (Oryza sativa ssp. japonica) plants pollinated by Oenothera biennis exhibited numerous morphological and developmental traits. Some variant individuals appeared in generation D2. From generations D3 to D6, a large number of variants were observed, showing distinct variable traits including giant embryos. Statistical analysis on D6 lines showed significant differences between progenies and their rice parental line in several main traits, including plant height (49.2–164.5 cm), panicle number (12.1–38.2), panicle length (20.3–30.3 cm), length of sword leaf (13.8–57.5 cm), leaf width (11.1–25.2 mm), grain number of main panicle (142.0–367.0), percentage of seed setting (0.8–99.0%), 1000-grain weight (19.7–33.8 g) and time from germination to panicle emergence (90.0–108.0 days). Most of the variable coefficients were above 20% (the highest was 40.8%). Through pedigree selection of these plants, genetically stable lines were obtained, which are useful for rice breeding. Results from amplified fragment length polymorphism (AFLP) analysis showed that several rice lines contained extensive genetic variations, which included disappearance of rice parental bands and/or appearance of novel bands.

Three efficient COD degrading bacteria were screened in the aeration tank of Zhengzhou Wulongkou municipal wastewater treatment plant by conventional isolation and purify methods. One of them (the isolate 4#) was short-rod-shaped, gram negative and the 16S rRNA sequence showed that it is closely related to Acinetobacter. The isolate 18# was rod-shaped, gram negative and the closest relative is Escherichia. The isolate 28# was short-rod-shaped, gram negative too, phylogenetically, the closest relative of isolate 28# is Aeromonas. When the isolates or the mixture of activated sludge and the efficient bacteria were added to municipal wastewater treatment system, the efficiency of COD removal was obviously increase. The result indicates that it is feasible to use the efficient bacteria for bioaugmentation.

Using conventional isolation and purifying methods, eight microbial flocculant-producing bacteria were screened from active sludge of Zhengzhou Wulongkou wastewater treatment plant. One of them was proved to have high flocculating capability through re-screened and was named as the isolate 127#. Selecting the isolate 127# as the research object, the optimal culture medium and culture conditions of this bacterium were established as follows: saccharose 20 g/L, sodium nitrate 1.5 g/L, dipotassium hydrogen phosphate 1 g/L, potassium chloride 0.5 g/L, heptahydrate magnesium sulfate 0.5 g/L, ferrous sulfate 0.01 g/L; pH 12, temperature 30degC, shaker rotate speed 150 r/min, culture time 74 h. Under the optimal culture condition, it has perfect flocculating ability on kaolin suspension. The flocculating ratio was up to 94.98%.

This paper proposes a GMPLS survivability scheme based differentiated services. This algorithm which is based on three normal GMPLS survivability scheme models (namely global protection, reverse backup and local repair) chooses evaluation function to decide in advance most suitable restoration model for working path of each kind of traffic, then only employs it to calculate the backup path of working path, in order to gain the aim of differentiated restoration for different service traffic.