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To evaluate diagnostic accuracy and reproducibility of 320-slice dynamic volume computed tomography (CT) for measuring left ventricular end diastolic volume (EDV), left ventricular end systolic volume (ESV), stroke volume (SV) and left ventricular ejection fraction (EF) during the cardiac cycle, we

Objectives To detect the effect of Sini Decoction on the expression of Sirt-1 and eNOS in EAhy926 cell injured by homocysteine. Methods Model of EAhy926 cell injured by homocysteine was made,the protection on the EAhy926 cell of Sini Decoction with different dosages were observed. NO concentration of cell culture fluid was detected by nitrate reductase method, effect of Sini Decoction on the expression of protein of Sirt-1 and eNOS in EAhy926 cell were observed by Western-blot, and effect of Sini Decoction on the expression of mRNA of Sirt-1 and eNOS in EAhy926 cell were observed by fluorescent quantitation PCR. Results After Model of EAhy926 cell injured by homocysteine was made, we found that cultured with 0.5,1.0,2.0,4.0,8.0 μmol/L homocysteine, cells grew less than cultured with normal culture medium, and with the increase of homocysteine concentration, the number of attached cell grew downwards obviously, as culturing with homocysteine 4.0 μmol/L for 24h did lower damage to cells and could induce effective cell injuring, it was made to be the model of injury. To detect the effect of Sini Decoction on EAhy926 cell injured by homocysteine, well growing EAhy926 cells were cultured in culture plate. 24 h later, cells were cultured with DMEM medium containing 2% fetal calf serum for 8 hours to make cells hungry, then cultured with medium containing Sini Decoction 0, 0.25, 0.5, 1.0g/ml respectively for 30 minutes,then cultured with medium containing homocysteine 4.0 μmol/L for 24 h. It was found that, compared with control group, attached cells in Sini Decoction groups grew better, and attached cells in Sini Decoction 1.0g/ml plus homocysteine 4.0 μmol/L group grew best. 4.0 μmol/L. Detected by nitrate reductase method, it was found that compared with control group, there was no obvious change of NO concentration of cell culture fluid in Sini Decoction 1.0 g/ml group, but in homocysteine 4.0 μmol/L medol group, NO concentration of cell culture fluid decreased oberviously, and in Sini Decoction groups, NO concentration of cell culture fluid increased, and in Sini Decoction 1.0g/ml plus homocysteine 4.0 μmol/L group it was the most obvious ( p p p Conclusions Homocysteine may injure EAhy926 cell by suppressing the expression of Sirt-1 then suppressing the expression of eNOS system, while Sini Decoction may protect EAhy926 cell by enhancing the expression of caveolin-1 then enhancing the expression of eNOS system.

Objectives To investigate the effects of Tongxinluo and Homocysteine (Hcy) on proliferation of human umbilical vein endothelial cells (Huvecs) in vitro . Methods Isolation,culture and identification of Huvecs:With the informed consents of puerperants, the normal fetal umbilical cords were obtained through uterine-incision delivery in Third Affiliated Hospital, Sun Yat-sun University. Huvecs were isolated by Percoll density gradient centrifugation from fetal umbilical cords with digestion of collagenase type perfusion, and then suspended in Medium 199 supplemented with 20% FBS, 2 mM L-glutamine, 50 μg/ml endothelial cell growth factor, 100 μg/ml heparin and 100μg/ml streptomycin cultured in 0.05 volume fraction of CO2 incubator at 37°. The culture solution was changed after 24 hours, Huvecs cultured to 80% confluence, the passage was developed with 0.25% trypsinase and 0.02% EDTA. After the Huvecs were identified by flow cytometry with the cell marker CD34, The second or third passage was used for study. MTT assay was used to detect cell viability: Huvecs during the logarithmic phase were inoculated in 96-well plates with Medium 199 in 0.05 volume fraction of CO2 incubator at 37° for 48h, at 80% confluence, incubated in serum-free medium,and divided into two groups, eachgroup including eight parallel wells, blank control group was untreated, he latter cultured and added with Hcy of the concentrations of 0.1, 0.25, 0.5, and 1.0 mmol/L respectively,or Hcy 0.5 mmol/L+Tongxinluo of the concentrations of 0.1, 0.5, 1.0, and 2.0mg/ml. After the cells were cultured for 24 hr, 5 g/L MTT solution (20μL) was added, then dimethyl sulfoxide was used for vibration and dissolvement. Optical density of each hole was detected by using enzyme-linked immunosorbent assay to analyse different concentrations of Hcy and Tongxinluo on the proliferation of human mammary stemscells. Results Hcy of different doses inhibited the viability of Huvecs dose-dependently, the Optical density were 0.526 ± 0.017, 0.439 ± 0.010, 0.258 ± 0.008, and 0.209 ± 0.004 respectively, lower than that (0.578 ± 0.023) of blank control group (all P P P Conclusions Hcy can inhibited the viability of Huvecs dose-dependently, and this effect is most prominent at the concentrations of 0.5 mmol/L for 24 hours; Tongxinluo can enhance the proliferation activity of Huvecs, and this effect is most prominent at 1.0mg/ml for 24 hours.