1. The effect of active components from citrus fruits on dentin MMPs
- Author
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Fan Yu, Jihua Chen, Zhengya Liu, Fang Li, Yu Haohan, and Ling Zhang
- Subjects
0301 basic medicine ,Naringenin ,Citrus ,Surface Properties ,In Vitro Techniques ,Matrix metalloproteinase ,03 medical and health sciences ,Hesperidin ,chemistry.chemical_compound ,Hydroxyproline ,0302 clinical medicine ,Microscopy, Electron, Transmission ,stomatognathic system ,medicine ,Dentin ,Humans ,General Dentistry ,Flavonoids ,Chromatography ,Chemistry ,Chlorhexidine ,Hesperetin ,food and beverages ,030206 dentistry ,Cell Biology ,General Medicine ,Matrix Metalloproteinases ,030104 developmental biology ,medicine.anatomical_structure ,Otorhinolaryngology ,Distilled water ,Collagenase ,Molar, Third ,medicine.drug - Abstract
Objectives This study was aimed to evaluate the anti-matrix metalloproteinases (MMPs) ability of active components from citrus fruits (hesperetin: Hst, hesperidin: Hsd and naringenin: Nge). Methods Inactivation effects of citrus flavonoids (Hst, Hsd, Nge) at different concentrations on soluble collagenase were measured using a fluorometric assay. Matrix-bound endogenous MMPs activity was evaluated via dry mass loss and hydroxyproline (HYP) release of demineralized human dentin. Demineralized dentin beams were pretreated with 500 μg/mL citrus flavonoids for 10 min. Chlorhexidine (CHX) was used as inhibitor control. Beams pretreated with distilled water served as blank control. Dentin slabs were used for in situ zymography and evaluated under confocal microscopy. Ultrastructure of demineralized collagen fibers was exhibited by Transmission Electron Microscopy (TEM). Results Citrus flavonoids exhibited inactivation function on soluble MMPs and the extent of inactivation increased in a dose-dependent manner. The inactivation percent of citrus flavonoids reached above 90% at the concentration of 500 μg/mL. Compared with control group, citrus flavonoids pretreated demineralized dentin beams exhibited less dry mass loss, lower hydroxyproline release and more intact collagen architecture after 15 days storage. Dentin samples pretreated with citrus flavonoids showed lower enzymes activities in in situ zymography. Conclusions Hst, Hsd or Nge have anti-MMPs ability and can preserve dentin collagen from degradation. Clinical Significance: Hst, Hsd and Nge may have the potential to be used in dentin bonding systems and improve the resin-dentin bonding durability.
- Published
- 2017