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Objective To compare the inflammatory reaction of peritoneal macrophage after Pg⁃LPS stimulated in healthy rabbit and hyperlipidemia rabbit. Methods 12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high⁃fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups: control, 1 μg/mL Pg⁃LPS, and 1 μg/mL E.coli⁃LPS. After 24 h treatment, the expressions of C⁃reaction protein (CRP), interleukin⁃1β (IL⁃1β), interleukin⁃6 (IL⁃6) , interleukin⁃8 (IL⁃8), and tumor necrosis factor⁃α (TNF⁃α) were detected by realtime PCR. Results The levels of CRP, IL⁃1β, IL⁃6, IL⁃8, and TNF⁃α were higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg⁃LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P < 0.05). Conclusion Pg⁃LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.