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As a chronic and infectious disease, dental caries has become a part of the heavy burdens of individuals and society, which is featured with tooth mineral loss and mainly caused by oral bacteria. Anti-caries agent should be safe, and capable of both high bacterial inhibition and remineralization, yet very few ones can meet these requirements simultaneously. In this study, a silver-doped amorphous calcium phosphate (Ag-EACP) is prepared by a biomimetic enzymatic reaction, with the in vivo energy storage biomolecule adenosine triphosphate disodium (Na2ATP) as the phosphorus source. Ag-EACP has an amorphous phase, with high content of ATP. The doped silver in the form of Ag+ ion is stabilized against transformation, can help adjust the size of Ag-EACP. The Ag-EACP can effectively inhibit the growth of Streptococcus mutans and Lactobacillus acidophilus, two main cariogenic pathogens, both in planktonic and free states, and suppress the formation of bio-film. Moreover, this nanocomposite generates significant remineralization and micro-hardness recovery of initial caries lesion. Ag-EACP shows great anti-caries effects on initial caries lesions and is promising for caries prevention in dentistry.

Biomineralization is a set of processes whereby living organisms generate hierarchically structured organic–inorganic materials. Studies on biomineralization not only elucidate the natural process of biomineral formation but also contribute to the development of functional biomaterials. However, the function of small molecules in the process has not been clarified. In this study, phosphoserine (PS) was used as a phosphorus source to prepare biomimetic minerals, and a new flower-like organic–inorganic complex of calcium PS (CaPS) was obtained. The CaPS samples were self-assembled plate-like crystals in the first 10 min. Then, over the next 5 h of the reaction, the plate-like crystals were broken down into fine granular nanoparticles. The chemical and thermal properties of CaPS were characterized, and the results indicated that the CaPS was or had a similar structure to Ca(C3H6NO6P)·H2O. X-ray absorption near edge structure (XANES) and extended X-ray absorption fine structure spectra (EXAFS) were obtained, which clearly revealed 6-fold-coordinated Ca-O bonds with lengths of 2.319 (±0.0147) A, 2.358 (±0.0147) A, and 2.435 (±0.0147) A. Furthermore, the results of a CCK-8 assay indicated the high biocompatibility of CaPS. When the samples were co-cultured with mouse preosteoblast cells (MC3T3-E1), CaPS exhibited good bioactivity to promote the osteoblast differentiation of cells. Owing to its excellent chemical and biological properties, the as-prepared CaPS complex is promising for various biomedical applications.

Background: To evaluate the predictive value of serum HBV DNA, HBV RNA, HBcrAg, HBsAg, intrahepatic HBV DNA, and cccDNA for HBeAg clearance and seroconversion during long-term treatment of nucleos(t)ide analogues (NAs) in patients with chronic hepatitis B (CHB).Method: A single center, prospective cohort of CHB patients enrolled between June 2007 and July 2008 was used for this study. Serum HBV RNA levels were retrospectively measured at baseline, 6, 12, 24, 36, 48, 60, 72, and 84 months post-NAs treatment. Serum HBsAg and HBcrAg levels were quantified at baseline, month 6, 60, and 72. Histological sample from liver biopsy at baseline and month 60 were analyzed for intrahepatic HBV DNA and cccDNA.Results: Eighty-three HBeAg patients were enrolled with an median follow-up time of 108 months (range 18-138 months). Of them, 53 (63.86%) patients achieved HBeAg clearance, and 37 (44.58%) achieved HBeAg seroconversion. Only baseline HBV RNA was independently associated with HBeAg clearance (OR=0.50, 95%CI 0.309-0.809, P=0.005) and seroconversion (OR=0.689, 95% CI 0.513-0.925, P=0.013). The independent negative association with HBeAg clearance and seroconversion remained for HBV RNA levels at month 6 (OR=0.42, 95%CI 0.248-0.714, P=0.001; OR=0.44, 95%CI 0.260-0.744, P=0.002) and month 12 (OR=0.39, 95%CI 0.253-0.592, PP=0.001). The AUC of baseline HBV RNA for predicting the HBeAg clearance and seroconversion were 0.81 (95%CI: 0.70-0.89) and 0.68 (95%CI: 0.56-0.78), respectively, higher than that of HBV DNA, HBsAg and HBcrAg.Conclusion: Lower serum HBV RNA at baseline, month 6 and 12 post NAs treatment could predict HBeAg clearance and seroconversion during long-term NAs treatment.

A rod-shaped, Gram-negative staining strain, FBM22

This study focused on the role and mechanism of IGF2BP1 in cell cisplatin resistance of oral squamous cell carcinoma.Low-dose intermittent induction method was used to induce cisplatin-sensitive oral squamous cell carcinoma cell line HN30, and establish cisplatin-resistant cell line HN30/DDP. Western blot was used to detect the protein expression level of IGF2BP1 in parental and resistant cell line. Knockdown or overexpression of IGF2BP1 by RNAi and lentivirus transfection method was utilized to analyze the effect of decreased or increased the gene expression of IGF2BP1 on cisplatin resistance. MTT method was used to detect the change of IC50. Statistical analysis of data was performed using SPSS 17.0 software package.Cisplatin-resistant oral squamous cell carcinoma cell line was successfully established. The IC50 of the drug-resistant cells was significantly higher than that of the parental cells. Knocking down the expression level of IGF2BP1 in drug-resistant strains reduced cell resistance; on the contrary, after overexpression of IGF2BP1 in parental cells dramatically increased cisplatin resistance. Mechanically, activation of Akt signaling pathway was the key factor mediating IGF2BP1 to promote cisplatin resistance in oral squamous cell carcinoma.IGF2BP1 is significantly associated with cisplatin resistance in oral squamous cell carcinoma. IGF2BP1 can promote cisplatin resistance of oral squamous cells by activating downstream Akt signaling pathway.

The seek of bioactive materials for promoting bone regeneration is a challenging and long-term task. Functionalization with inorganic metal ions or drug molecules is considered effective strategies to improve the bioactivity of various existing biomaterials. Herein, amorphous calcium magnesium phosphate (ACMP) nanoparticles and simvastatin (SIM)-loaded ACMP (ACMP/SIM) nanocomposites were developed via a simple co-precipitation strategy. The physiochemical property of ACMP/SIM was explored using transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD) and high-performance liquid chromatograph (HPLC), and the role of Mg2+ in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure (XANES). After that, the transformation process of ACMP/SIM in simulated body fluid (SBF) was also tracked to simulate and explore the in vivo mineralization performance of materials. We find that ACMP/SIM releases ions of Ca2+, Mg2+ and PO43−, when it is immersed in SBF at 37°C, and a phase transformation occurred during which the initially amorphous ACMP turns into self-assembled hydroxyapatite (HAP). Furthermore, ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells. For the in vivo studies, lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in calvaria. ACMP/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after 12 weeks. The bioactive ACMP/SIM nanocomposites are promising as bone repair materials. Considering the facile preparation process and superior in vitro/vivo bioactivity, the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications.

Loess deposition from wind is one of the major sources of soil development in many regions including northeastern China. The dust deposition on snow from 6 sites in Shenyang, northeastern China was collected and characterized physically and chemically to determine its origin (modern vs. re-deposited loess). The origin and characteristics of the dust deposition were evaluated through comparisons with possible sources of sand desert areas based on elemental compositions, such as the A-CN-K (Al2O3-CaO + Na2O-K2O) ternary plot and ratios of Al2O3/TiO2, Fe2O3/TiO2, and Fe2O3/Al2O3. The texture and composition results showed that the dust deposition was most recent and not re-deposited loess. The dust deposition was predominantly silt-sized grains (2–50 μm) and had greater amounts of Na2O (1.7% vs. 1.5% average), MgO (2.1% vs. 1.2%), and CaO (3.2% vs. 1.2%) when compared to the local soil. Its mineral composition was mainly quartz, feldspar, and mica. The magnetic susceptibility of the dust deposition was 30.9 × 10−8 m3/kg and lower than the local soil which was 40.4 × 10−8 m3/kg. Based on the A-CN-K ternary plot, the dust had a weaker weathering intensity than the local soil, loess-paleosol sequences, and red clay. This indicated that the dust was not a re-deposited loess. Furthermore, according to the discriminating analysis using ratios of Al2O3/TiO2, Fe2O3/TiO2, and Fe2O3/Al2O3, the source for the dust was traced from the deserts of south Mongolia, Tengger, Gulban Tungut, and Taklimakan. The two primary factors transporting the dust were the Mongolia Cyclone and westerlies. The dust was blown into the atmosphere, and transported by the atmospheric circulation from west to east and/or from northwest to southeast, which was accompanied also by a cold front cyclone moving southeast towards the study area. The origin, transportation, and deposition process of the dust deposition in this study provides further evidence about the origin of the loess and its likely role in soil development in Shenyang, northeastern China.

Water movement over and through soil is largely driven by topography and soil management across landscapes. This research tested the hypothesis that the water movement determines the potential for P and Ca redistribution and pH variance across landscapes. This hypothesis was evaluated by using digital elevation model-derived terrain attributes in fields after 55 yr of broiler litter applications on pastures in Smith County, Mississippi. Results show that soils receiving broiler litter had mean Mehlich-3 P levels of 1221.8 mg kg⁻¹ at 0- to 15-cm depth and 618.6 mg kg⁻¹ at 15- to 30-cm depth, and Ca with mean values of 768.3 and 645.0 mg kg⁻¹ at 0- to 15-cm and 15- to 30-cm soil depths, respectively. Across fields, soils in areas of predicted convergent flow contained higher P, Ca, and lower pH values in the upper 0 to 15 cm, suggesting contributions via surface overland flow from areas with higher elevation and lower slope gradient. On the other hand, soils in areas with lesser slope and higher elevation also contained high levels of P, Ca, and pH for the subsurface soil depth, suggesting that vertical flow of water on this landscape is a mechanism for movement of P and Ca deeper in the profile. The incorporation of topographic characteristics across fields offers promising results that may be incorporated into improved P indices and management, making them more robust indicators of P mobilization to waterways.

One-dimensional hydroxyapatite (HA) particularly mimics the structure of mineralized collagen fibrils and displays superior mechanical properties such as toughness. Herein, we report Se-doped HA/chitosan (Se-HA/CS) biopapers constructed with self-assembled Se-doped HA nanowires and chitosan. The Se-HA/CS biopapers with high flexibility and manufacturability can not only be further processed into arbitrary shapes by folding or using scissors but also display high performances in in vitro/vivo anti-bone tumor studies. The Se-HA/CS biopapers are more inclined to inhibit the growth of tumor cells (HCS 2/8 and SJSA cells) than that of normal human bone marrow stromal cells (hBMSCs). The potential mechanisms of this meaningful anti-tumor effect were investigated, such as reactive oxygen species accumulation and the activation of apoptosis and the underlying signal pathway involved (including caspase family, Bcl-2 family and JNK/STAT3). The results demonstrate that Se-HA/CS biopapers may inhibit the growth of HCS 2/8 and SJSA cells by synchronously inducing JNK activation and STAT3 inhibition and consequently promote the apoptosis of these cells. Furthermore, the in vivo anti-tumor studies confirm that the Se-HA/CS biopapers obviously suppress the growth of patient-derived xenograft tumor models.

Background Alisol A 24-acetate (AA-24-a), one of the main active triterpenes isolated from the well-known medicinal plant Alisma orientale (Sam.) Juz., exhibits multiple biological activities including hypolipidemic activity. However, its effect on lipid metabolism in adipocytes remains unclear. The present study aimed to clarify the effect of AA-24-a on adipocyte lipolysis and to determine its potential mechanism of action using 3 T3-L1 cells. Methods We assayed the release of glycerol into culture medium of 3 T3-L1 cells under treatment with AA-24-a. Protein and mRNA expression and phosphorylation levels of the main lipases and kinases involved in lipolysis regulation were determined by quantitative polymerase chain reaction and western blotting. Specific inhibitors of protein kinase A (PKA; H89) and extracellular signal-regulated kinase (ERK; PD98059), which are key enzymes in relevant signaling pathways, were used to examine their roles in AA-24-a-stimulated lipolysis. Results AA-24-a significantly stimulated neutral lipolysis in fully differentiated adipocytes. To determine the underlying mechanism, we assessed the changes in mRNA and protein levels of key lipolysis-related genes in the presence or absence of H89 and PD98059. Both inhibitors reduced AA-24-a-induced lipolysis. Moreover, pretreatment with H89 attenuated AA-24-a-induced phosphorylation of hormone-sensitive lipase at Ser660, while pretreatment with PD98059 attenuated AA-24-a-induced downregulation of peroxisome proliferator-activated receptor-γ and perilipin A. Conclusions Our results indicate that AA-24-a promoted neutral lipolysis in 3 T3-L1 adipocytes by activating PKA-mediated phosphorylation of hormone-sensitive lipase and ERK- mediated downregulation of expression of perilipin A.

Peripheral nerve injuries represent a great challenge for surgeons. The conductive neural scaffold has experienced increasing interest because of its good biocompatibility and similar electrical properties as compared to those of a normal nerve. Herein, nerve conduits made from poly(d,l-lactide)-co-poly(ethylene glycol) and polypyrrole (20%, 30%, and 50%) (PELA–PPY) were prepared by electrospinning, and used in regeneration of peripheral nerve defects. The results of an in vitro experiment indicated a high biocompatibility for the as-prepared materials, supporting the attachment and proliferation of a rat pheochromocytoma PC-12 cell. Furthermore, the PELA–PPY nerve conduit implanted in the sciatic nerve defects (10 mm) of the Spraguee–Dawley rats for 12 weeks showed similar results with the autograft, while it demonstrated a better outcome than the PELA nerve conduit in electrophysiological examination, sciatic function index, total amount of regenerated myelinated nerve fibers, axon diameter, myelin thic...

The Chaoyang profile represents a rare multi-period, continuous and complete sequence of aeolian paleo-deposits with a stable sedimentary origin and multi-stage paleoclimatic cycles. Benchmark profiles including soil types at different pedogenic stages can be used for the recognition and classification of paleosols and paleoclimate reconstruction. The loess–paleosol sequence benchmark profile (LBP) is also helpful in comparing the results of paleoenvironment reconstruction from different ecological regions. In this study, a loess–paleosol profile derived from thick loess in Chaoyang city of Liaoning province, Northeast China, was investigated as a well-preserved LBP that included various paleosol types. To determine the nature and origin of the Chaoyang profile, the geographic, stratigraphic and morphological characteristics were described in the field. Bulk samples from 42 horizons were collected for chemical and physical analysis, and sub-sampling of 946 samples at 2 cm intervals from the surface to the bottom were taken to measure grain size distributions and magnetic susceptibility. Results showed that the 19.85 m thick loess–paleosol profile had been continuously deposited since 423 ka BP. The upper part (0–195 cm), or UPP, was predominantly of aeolian loess deposition origin but was mixed with water-reworked materials from a nearby secondary loess source. The middle part (195–228 cm), or MIP, was also indirectly affected by the water-reworking process through the leaching of materials from the overlying UPP. The lower part (228–1985 cm), or LOP, was characterized by four reddish stratigraphic layers interbedded with five yellowish ones, indicating several types of paleosols developed under different ecological environments. The multi-stage paleoclimatic cycles as evidenced by morphological and physical characteristics as well as age dating and magnetic susceptibility correlated well with the Lingtai section and LR04 benthic δ18O. Because of these attributes, the Chaoyang profile can be deemed as a benchmark loess–paleosol profile for the recognition and classification of paleosols and paleoclimate reconstruction in Northeast China. The differences in morphological and physical properties between paleosols and loess suggest different soil fertility and agronomic properties and need further studies to assess their functionality with climate fluctuation.

The internal source pollution of sediment is the main factor leading to the repetition of black-odorous river channels. In order to prevent this situation, a river channel in the Binhu District of Wuxi City was used as an experimental site. In-situ CaO

Algal organic matter (AOM) and natural organic matter (NOM) from a typical eutrophic lake were comprehensively investigated in terms of their physico-chemical property, components and disinfection byproduct formation potentials (DBPFPs). The relationships between specific chemical properties of AOM and NOM with their corresponding DBPFPs were further evaluated during chlorination. Results indicated that AOM had lower specific UV absorbance (SUVA) but richer organic nitrogen contents than NOM. Fluorescence excitation emission matrix spectroscopy further demonstrated that AOM were chiefly composed of aromatic protein-like and soluble microbial byproduct-like matters, while NOM were mainly contributed from humic acid-like and soluble microbial byproduct-like substances. Although the molecular weight (MW) distribution of AOM and NOM showed no significant difference, size-exclusion chromatography with organic carbon as well as organic nitrogen detection (LC-OCD-OND) revealed that AOM were concentrated with the fraction of building blocks and NOM had higher concentrations of biopolymers and humics (HS). Moreover, AOM displayed higher DBPFPs than NOM, especially for nitrogenous DBPFP (N-DBPFP). MW < 1 kDa fractions both in AOM and NOM contributed the largest proportion to the formation of carbonaceous disinfection byproducts (C-DBPs). In addition, Pearson correlation analysis showed that bulk parameter SUVA was significantly relevant to the formation potentials of trihalomethane both in AOM and NOM, but was ineffective for carbonaceous DBPFP (C-DBPFP) prediction. Dissolved organic nitrogen contents in biopolymer and HS characterized by LC-OCD-OND had strong correlations with N-DBPFPs from AOM and NOM, indicating that LC-OCD-OND quantitative analysis could improve the prediction accuracy of the DBP formation than bulk parameters during NOM and AOM chlorination.

Adenosine triphosphate (ATP) biomolecules play critial roles in the biomineralization process during the formation of amorphous calcium phosphate composites (ACPC), and ACPC is an important drug carrier due to its significant advantages of biocompatibility and biodegradability. Hence, studying the behavior of ACPC nanodrug carriers is crucial to investigate the structural regulation of biomimetic minerals and calcium phosphate (CaP)-based drug delivery systems. However, it is difficult to probe these interactions using traditional characterization methods. In this paper, XANES analysis together with STXM successfully provided a method to reveal the interaction of ATP and drug molecules with individual mesoporous ACPC. We found that the adenosine and phosphate groups of ATP biomolecules coordinated with Ca2+ and played critical roles in the formation of ACPC; drug molecules with the -COOH groups were linked to Ca2+via carboxylic acid groups primarily by electrostatic interactions, and the N-containing ring structures within the drug molecules also coordinated with Ca2+.

Magnesium silicate nanostructured biomaterials with good biocompatibility and high adsorption capacity for drugs and proteins are promising for applications in various biomedical fields. However, the applications of magnesium silicate nanostructured biomaterials in anticancer drug delivery and protein adsorption have rarely been reported so far. Herein, we report a facile strategy for the synthesis of magnesium silicate hollow nanospheres (MSHNSs) by a classical Stober method and a template based solvothermal process. The as-prepared MSHNSs have an ultrahigh specific surface area of 585.6 m2 g−1, ultrahigh hemoglobin (Hb) protein adsorption capacity (1262 mg g−1) and high doxorubicin (DOX) drug loading capacity (559 mg g−1). Moreover, the as-prepared MSHNS/DOX drug delivery system exhibits sustained and pH-responsive drug release performance. Compared with free DOX, the MSHNS/DOX drug delivery system exhibits higher anticancer activity in vitro, and thus it is promising for applications in anticancer treatment.

The development of multifunctional dental-restorative biomaterials with antibacterial activity and remineralization effect for damaged tooth repair is urgent since dental caries is still one of the most common tooth diseases in human beings. Herein, we report a facile strategy for the synthesis of gluey silver-calcium phosphate (GSCP) composites using the rapid microwave-assisted solvothermal method. The as-prepared GSCP composite is an organic-inorganic hybrid, and Ag+ ions display a significant influence on the formation of GSCP by interacting with adenosine triphosphate biomolecules. The as-prepared GSCP composite shows good antibacterial properties, in addition, it exhibits a great effect on sheltering dentinal canaliculi and improving the remineralization of dentine in the simulated saliva. The as-prepared GSCP composite is promising for various applications such as oral healthcare, especially, remineralization of dentine, and antibacterial applications.

Long-term weathering patterns in northeastern China are not well known due to few well-preserved terrestrial paleo-environmental archives in this area. The Chaoyang section is a typical well-preserved loess-paleosol sequence in northeast China with continuous deposition since 423 ka BP; the deposit thus represents an archive of climate change for this region. The sequence was geochemically characterized by major elemental compositions, elemental ratios, a ternary diagram of Al2O3-(CaO* + Na2O)-K2O, improved quantitative reconstruction and elemental distributions with respect to the average for the upper continental crust (AUCC). Another typical loess-paleosol sequence of Lingtai section from the central Chinese Loess Plateau was used for comparison with the loess-paleosol one. The similar AUCC-normalized major elemental distributions and strong correlation of the major elemental compositions between the loess-paleosol sequences indicate that they may have originated predominantly from a similar loess source. Based on the variability in chemical indices corresponding to soil magnetic susceptibility (SUS) and field observations, the loess-paleosol formation period of 423–77 ka BP was separated into eight sub-periods including four periods with greater chemical weathering intensity (paleosols) and four periods characterized by the relative lesser chemical weathering intensity (loess). Relatively intense desilication and fersiallitization primarily occurred in the loess-paleosol sequence from northeast China with greater losses of SiO2 (3.54% in average) and gains of Fe2O3 (0.77%) and Al2O3 (0.33%). Such processes also were reflected in an increase in the amount of K2O (0.41%). Ca and Na leaching was still predominant in the loess-paleosol sequence from the central Chinese Loess Plateau with greater losses of CaO (28.03%) and Na2O (14.03%). The sequence from northeast China records chemical weathering during 423–77 ka BP, which is comparable to the weathering cycles in the sequence from the central Chinese Loess Plateau.

The changes of soil particle size distributions (PSDs) can be used as a proxy for soil development intensity and an indicator of soil formation processes. The fragmentation fractal dimensions can describe PSDs of soils in a single parameter. The objective of this research is to use mass-based fragmentation fractal dimension models to describe PSDs and evaluate the relationship between the fragmentation fractal dimension values (DF) and pedogenesis in loess-paleosol. Based on the theory of fragmentation fractal dimension, particle size data from 837 eolian loess samples from the lower part of Chaoyang section uniform loess deposits were analyzed with mass-based models using linear regression and piecewise linear regression. A single power-law exponent can characterize the PSDs across the domain of 0.1–54.4 μm. The trend of fragmentation fractal dimension values is consistent with chemical weathering indices, magnetic susceptibility, and DF2 (close to 2). The energy dissipation for fragmenting larger particles from coarse silt (CSI) to fine silt (FSI) is identified in the volume and for fragmenting smaller particles from fine silt (FSI) to clay (CL) it is substantially on the surface. This research indicated that fragmentation fractal model is a good descriptor for the PSDs and DF can be a value quantifying the intensity of loess-paleosol pedogenesis.

One specific behavior can be synergistically modulated by different neural pathways. Medial septal (MS) cholinergic system innervates widespread cortical and subcortical regions and participates in pain modulation, but the underlying neural pathways are not fully understood. This study examined the contribution of MS cholinergic neurons and 2 neural pathways: MS-rostral anterior cingulate cortex (rACC) and MS-ventral hippocampal CA1 (vCA1), in modulating perceptual and affective pain behaviors in a mouse model of chronic inflammatory pain. We found that chronic pain activated MS cholinergic neurons and pyramidal neurons in the rACC, but suppressed pyramidal neuronal activities in the vCA1, all of which contributed to the maintenance of pathological pain. Chemogenetic inhibition of MS cholinergic neurons or the MS-rACC pathway inhibited rACC pyramidal neuronal activities and attenuated perceptual and affective dimensions of chronic pain. By contrast, chemogenetic activation of MS cholinergic neurons also produced analgesia, but by rescuing hypofunctional pyramidal neurons in vCA1. These results clearly demonstrate that the MS cholinergic system differentially modulates chronic inflammatory pain through MS-rACC or MS-vCA1 pathways. More significantly, our research provides evidence for a novel paradigm of neural circuit modulation: MS cholinergic inhibition and activation induce similar analgesia but through distinct neural pathways.

Cholinergic neurons in the medial septum (MS) participate in various cognitive and emotional behaviors, including innate anxiety. Chronic pain involves perceptual, cognitive and emotional components. Whether MS cholinergic system modulates pain-induced anxiety and the underlying neural circuits are involved remain unclear. In the present study, we showed that chemogenetic (DREADD) inhibition of MS cholinergic neurons relieved pain-induced anxiety-like behaviors in open field and elevated plus maze tests. Inhibiting the MS-rostral anterior cingulate cortex (rACC), but not the MS-ventral hippocampal CA1 pathway, achieved anxiolysis. These findings indicate the involvement of MS cholinergic system in modulating pain-induced anxiety-like behaviors.

Anisotropy correction is necessary during the processing of converted PSwave seismic data to achieve accurate structural imaging, reservoir prediction, and fracture detection. To effectively eliminate the adverse effects of S-wave splitting and to improve PSwave imaging quality, we tested methods for pre-stack migration imaging and anisotropic correction of PS-wave data. We based this on the propagation rules of seismic waves in a horizontal transverse isotropy medium, which is a fractured medium model that reflects likely subsurface conditions in the field. We used the radial (R) and transverse (T) components of PS-wave data to separate the fast and slow S-wave components, after which their propagation moveout was effectively extracted. Meanwhile, corrections for the energies and propagation moveouts of the R and T components were implemented using mathematical rotation. The PS-wave imaging quality was distinctly improved, and we demonstrated the reliability of our methods through numerical simulations. Applying our methods to three-dimensional and three-component seismic field data from the Xinchang-Hexingchang region of the Western Sichuan Depression in China, we obtained high-quality seismic imaging with continuous reflection wave groups, distinct structural features, and specific stratigraphic contact relationships. This study provides an effective and reliable approach for data processing that will improve the exploration of complex, hidden lithologic gas reservoirs.

Little is known about how Fe-Mn nodules vary in relation to pedogenetic horizons in soils derived from loess. In this investigation nodules were collected according to soil genetic horizons from a Fragiudalf in loess at Southeast Purdue Agricultural Center (SEPAC) in Indiana and physical, chemical, micro- and macromorphological features of nodules and their soil matrix were determined as a function of soil depth. The quantity, chemical composition, and micromorphology of the nodules varied with genetic horizons. The Ap horizon was found to contain the largest amount by weight of nodules (25.43 g/kg) and this was likely due to soil erosion. After the Ap horizon, the 2Btx1 and 2Btx2 horizons had the greatest content of Fe-Mn nodules by weight. Nodules had lower concentrations of SiO2, Al2O3, K2O, TiO2, MgO, Na2O, and CaO; in contrast Fe2O3, MnO, and P2O5 in nodules showed clearly greater concentrations than that in the corresponding soil material. The SiO2 content decreased with nodule size whereas Fe2O3 and MnO increased. The least depletion of SiO2 in nodules of 1–2 mm (N2) and 0.5–1 mm (N1) was in the Bt2 horizon, and was greatest in the 2Btx2. The Al2O3 had accumulated in the N2 and N1 nodules within the Ap horizon and in the N2 within the 2Btx2 while other horizons were depleted of Al2O3. Brown nodules had a small Mn/Fe ratio ( 1). The internal morphology of the nodules also varied with Mn/Fe ratios. The fungal hyphae or bacteria resulted in honeycomb, cell, cone, or dendritic structures. The cell and cone morphology required large Mn/Fe ratios, low pH, and high content of Ald. The scanning electron microscopy (SEM) analysis illustrated an elemental distribution trend such that the Mn was centralized in the nodule internal structure; whereas, Fe was concentrated towards the exterior. The band structure of nodules likely formed in response to seasonal wetting and drying as an accretionary process. Three basic formation stages for a well developed nodule can be inferred which correspond to different soil forming environments: 1) Fe enrichment for nucleus formation in wet soil, 2) Mn enrichment in an intense dry soil, and 3) Fe banding enrichment in a slightly dry soil. Nodule formation occurred synchronously with soil pedogenesis and can therefore be used as a proxy for understanding soil pedogenesis and the environment under which it occurred.

Iron-manganese (Fe-Mn) concentrations are common morphological indicators of soil oxidation-reduction reactions and are widely used to infer soil hydrology. The factors determining occurrence and distribution of Fe-Mn concentrations at the continental scale have been rarely studied. The objective of this study was to explore the distribution of Fe-Mn concentrations in upland loess soils and identify their favorable environment conditions in the mid-continental North America. A total of 3966 soil profiles were collected from the National Soil Information System database (NASIS). A decision tree (DT) model was used to analyze the spatial relationships between Fe-Mn concentration formation and its relevant environmental factors (climate, soil properties, and land use/land cover), and to identify key factors of Fe-Mn concentrations distribution and occurrence in upland loess soils. The DT model was evaluated with 25% validation dataset with an accuracy of 79%. Results showed that Fe-Mn concentrations were more likely to occur in mesic areas with large precipitation and low internal soil drainage rates, compared to frigid and thermic areas with low precipitation and large internal soil drainage rates. Annual precipitation and temperature were identified as the most important climatic factors influencing the occurrence of Fe-Mn concentrations and showed a complex interaction with soil properties at multiple scales. Soil properties related to potential wet to saturated soil conditions were also important for the occurrence of Fe-Mn concentrations. Results demonstrated the importance of using knowledge of pedogenesis in the selection of environmental covariates and the interpretation of DT model results. Understanding where Fe-Mn concentrations tend to form in the landscape will contribute to improving decisions about proper use of soil that may be limited by wetness and possible inferences about climate influences.

Hydroxyapatite/collagen (HAP/Col) composite porous scaffolds have been extensively investigated in the field of bone tissue engineering due to their similar chemical composition to that of natural bone and good biocompatibility. However, the relatively poor mechanical properties of the HAP/Col composite porous scaffolds limit their application in bone regeneration. In order to overcome these limitations, the hierarchical porous ultralong hydroxyapatite nanowires (UHANWs)/collagen (Col) composite scaffolds with excellent mechanical properties are synthesized by freeze-drying the suspension containing UHANWs and collagen. Compared with the HAP nanorods (HANRs), the highly flexible UHANWs can interweave with each other to construct a three-dimensional fabric-like structure in the collagen matrix of the UHANWs/Col scaffolds and significantly improve the mechanical properties of the scaffolds. The Young's modulus of the 70 wt% UHANWs/Col scaffold is about 4 times that of the pure collagen scaffold and 63 times that of the 70 wt% HANRs/Col scaffold. More importantly, in the absence of the collagen matrix, the pure UHANWs inorganic porous scaffold can be synthesized and exhibits relative good mechanical properties. However, the HANRs/Col scaffolds with HANRs weight fractions more than 70 wt% are easily cracked during the fabrication process. Furthermore, compared with the pure collagen and 70 wt% HANRs/Col scaffolds, the 70 wt% UHANWs/Col scaffold exhibits a superior biocompatibility and excellent ability to promote cell adhesion and spreading on the surface of the scaffold. In consideration of their outstanding mechanical properties and excellent cellular attachment performance, the as-prepared hierarchical porous UHANWs/Col scaffolds are promising for applications in various biomedical fields such as bone defect repair.

AIM:To evaluate the percentage of cutting fluctuations of central corneal thickness(CCT)intraoperative used low concentration(0.02%)mitomycin C(MMC)after laser-assisted subepithelial keratomileusis(LASEK). METHODS: In this prospective study, low and medium myopia group(spherical equivalent≤6.0DS)has 138 patients(276 eyes). Low concentration MMC used topically in 69 patients(138 eyes)randomized after excimer laser ablation; the another traditional LASEK as control. High myopia group(6.0DSt test and their repeatability was assessed using the coefficient of variation(CV).RESULTS: At 3mo after operation, the difference of central corneal thickness and cutting value between different treatment groups was statistically significant(PPPPCONCLUSION: The study on percentage of cutting fluctuations of central corneal thickness, further confirmed low concentrations MMC in reducing postoperative corneal stromal proliferation reaction, inhibition of haze production.

Hydrogels with 3-dimentional cross-linked structures are widely used in various biomedical fields such as bone repair scaffolds, drug carriers and biosensors. However, the applications of hydrogels are usually restricted because of their poor mechanical properties. Currently, nanocomposites, double network systems, hydrophobic association, macromolecules, and nanoparticles are commonly adopted as cross-linking agents to enhance mechanical properties of hydrogels. In this work, ultralong hydroxyapatite nanowires (HANWs) with lengths of several hundred microns are prepared and used to enhance the mechanical properties of sodium alginate (SA)-based hydrogels. Using divalent calcium ions as the cross-linking agent, the hybrid HANWs/SA hydrogels containing various percentages of HANWs are obtained. The as-prepared HANWs/SA hybrid hydrogels have a porous structure with pore sizes ranging from about 200 to 500μm. The mechanical properties of SA hydrogels can be significantly improved by incorporating HANWs. The maximum compressive modulus (E50%) and tensile Young's modulus of the hybrid hydrogel (HANWs/SA=2:1) are as high as 0.123MPa and 0.994MPa, which are about 162% and 614% those of the pure SA hydrogel, respectively. Due to the enhanced mechanical properties and high biocompatibility, the as-prepared HANWs/SA hybrid hydrogels have promising applications in various biomedical fields such as bone defect repair.

High-strength flexible inorganic paper with fire-resistant and adiabatic properties is highly demanded in various high-temperature applications. However, constructing inorganic paper that not only has high strength and high flexibility at room temperature but also can prevent the high-temperature-induced friability is still a great challenge. Inspired by the hierarchical structure and excellent mechanical properties of the tooth enamel, we have developed a systematic approach for the bottom-up fabrication of multi-hierarchical fire-resistant hydroxyapatite (HAP) nanowire paper with balanced tensile strength and flexibility that includes four steps: (1) the synthesis of monodisperse HAP nanowires from the molecular level to the nanoscale; (2) the self-assembly of HAP nanowires into long fibers and two-dimensional (2D) nanowire networks from the nanoscale to the mesoscale; (3) the layered assembly of 2D nanowire networks into the highly flexible high-strength fire-resistant paper from the mesoscale to the macroscale; (4) reinforcing the HAP nanowire paper with inorganic additives to enhance the tensile strength and to overcome the high-temperature-induced pulverization. By adopting this strategy, the mechanical properties of the fire-resistant HAP nanowire paper are greatly improved. The experimental results show that the tensile strength of the as-prepared HAP nanowires-based inorganic paper is greatly enhanced to ≈15 MPa, which is close to that of the commercial copying paper, and the A4-sized HAP nanowires-based inorganic paper is highly flexible and can be directly printed using a commercial printer. Owing to the synergistic effect of all components and the unique hierarchical structure, the as-prepared fire-resistant HAP nanowire paper can also preserve well its high flexibility even under high-temperature conditions.

Cholinergic neurons in the medial septum (MS) participate in a variety of cognitive and emotional behaviors. Some studies but not others show that lesions or inhibition of the MS reduce anxiety-like behaviors and locomotive exploration in rats. However, these conclusions come from manipulations that are either irreversible or non-specific to cholinergic neurons, casting doubt on their validity. With DREADD (designer receptors exclusively activated by designer drugs), we temporarily and reversibly inhibited cholinergic neurons in the MS. We observed consistent anxiolytic effects of MS cholinergic inhibition in the novelty-suppressed feeding test, the marble burying test and the elevated plus-maze test, as well as increased exploratory activities in the open field test. These findings confirm an excitatory role of the MS cholinergic neurons in the control of innate anxiety, and reconcile conflicting findings from previous studies using irreversible lesions or non-specific inhibition.

Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Internal Transcribed Spacer (ITS) sequences based on primers ITS5-1737 and ITS2-2043R to investigate the endophytic fungi diversity of P. sinese roots at the maturity stage, as collected from four provinces (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China). The ITS sequences were processed using QIIME and R software. A total of 374,875 effective tags were obtained, and 708 operational taxonomic units (OTUs) were yielded with 97% identity in the five samples. Ascomycota and Basidiomycota were the two dominant phyla in the five samples, and the genera Khuskia and Heydenia were the most abundant in the FJ and XJ samples, respectively, while the most abundant tags in the other three samples could not be annotated at the genus level. In addition, our study revealed that the FJ sample possessed the highest OTU numbers (242) and the NS sample had the lowest (86). Moreover, only 22 OTUs were present in all samples simultaneously. The beta diversity analysis suggested a division of two endophytic fungi groups: the FJ sample from the south of China and the other four samples from north or northwest China. Correlation analysis between the environmental factors and endophytic fungi at the class level revealed that Sordariomycetes and Pucciniomycetes had extremely significant positive correlations with the total carbon, annual average precipitation, and annual average temperature, while Leotiomycetes showed an extremely significant negative correlation with quick acting potassium. The results revealed significant differences in the root endophytic fungi diversity of P. sinese in different provinces and might be useful for growth promotion and biomass production in the future.

A nano-calcium peroxide (nCaO2) powder with a purity of 89.1% was prepared using an improved traditional method. Then, the as-prepared nCaO2 was used as the source of hydrogen peroxide (H2O2) for the Fenton-like degradation of diclofenac sodium (DCF). The results showed that nCaO2 performed better for DCF removal when compared to nCaO2 prepared by a conventional method and commercial calcium peroxide (CaO2). Further experimental results indicated that 97.5% of DCF could be removed in 180 min at a nCaO2/Fe2+-EDTA/DCF molar ratio of 16/8–8/1, which was more efficient than in the H2O2/EDTA-Fe2+/DCF and nCaO2/Fe2+/DCF systems. The best removal rate of DCF was at pH 6.0, unlike previous claims that stated that the lower the pH in the buffer system, the better the degradation of DCF. In addition, the influence of water quality parameters, such as Cl−, NO3−, SO42−, HCO3−, and humic acid (HA), on DCF removal were evaluated. A free radical masking experiment revealed the existence of hydroxyl radical ( OH), superoxide radical (O2− ) and singlet oxygen (1O2), and indicated that the degradation of DCF was mainly due to oxidation caused by OH. Electron paramagnetic resonance (EPR) studies for different systems and different active oxygen species were carried out, and it was further confirmed that OH radicals have high intensity in the Fenton-like system based on nCaO2. EPR results also showed that the addition of EDTA can promote the production of OH. According to the identification of the dominant reactive species and GC–MS, the possible theoretical DCF degradation pathways were proposed.

Tephra layers can serve as ideal datable horizons to provide invaluable datums for supplementing research on loess-paleosol sequences. The objective of this research was to evaluate a typical loess profile interbedded with a macroscopic tephra in northeastern China to better understand its formation to determine the potential impact of volcanism on climate change, as evidenced by loess pedogenesis. Geochemical and physical properties of the profile were determined and compared with loess materials from other areas to address its characteristics and potential significance for understanding climate variability. Results showed the tephra layers with overlying loess horizons were uniform in parent material, as inferred from evidence such as uniform Ti/Zr ratios with depth, similar rare earth element distribution patterns, and major elemental distributions with depth. The volcanic properties in the bottom of the profile, including morphology, high phosphate adsorption capacity (PAC) and pH (NaF) values, further supported the speculation that the depositions of aeolian dust and volcanic materials were synchronous with pedogenic processes. According to the comparison of magnetic susceptibility of the typical profile with the global δ18O curve, the local paleoclimate showed an increase in temperature in warmth and wetness, which may have been affected by volcanism. Research on the loess profile interbedded with a macroscopic tephra could improve our knowledge of loess formation and deepen our understanding of the impact of volcanism on climate changes.

The construction of a functional drug delivery system to reverse the multidrug resistance (MDR) of bone tumors in cases of failed chemotherapy remains a challenge. Herein, we demonstrate a selenium-doped calcium phosphate (Se-CaP) biomineral with high biocompatibility, biodegradability and pH-sensitive drug release properties. Se-CaP may not only serve as an effective drug-carrier to enhance the uptake of doxorubicin (DOX), but may also synchronously induce caspases-mediated apoptosis of osteosarcoma by generating intracellular reactive oxygen species (ROS). Furthermore, in vitro and in vivo studies obviously demonstrate that Se-CaP can reverse the MDR of osteosarcoma by down-regulating the expression of MDR-related ABC (ATP binding cassette) transporters proteins (ABCB1 and ABCC1). Finally, DOX-loaded Se-CaP can significantly inhibit DOX-resistant MG63 (MG63/DXR) tumor growth in nude mice. Considering its biomimetic chemical properties, the Se-CaP biomineral, with the multiple functions mentioned above, could be a promising candidate for treating bone tumors with MDR characteristics.

Objective Weight losses between a group of participants assigned to a weight loss program based in the community [i.e., specifically the methodology used by Weight Watchers (WW)] and a Nutrition Education (NE) control group were compared in this study. Methods In this 6-month trial, 300 participants with overweight or obesity were recruited from Beijing city, China, and randomly assigned to the WW or NE group. Weight, waist circumference, and biochemical parameters were assessed at baseline and 6 months. Results At 6 months, the majority of participants (79% for WW; 89% for NE) completed the study. WW participants lost significantly more weight than the NE group (−4.2 ± 5.6 kg vs. −0.6 ± 3.6 kg). More WW participants lost 5% or 10% of their starting weight [≥5%: 52.0% of WW participants vs. 11.3% of NE participants (odds ratio 8.15, 95% CI: 4.43–14.97)]; [≥10%: 26.0% of WW vs. 3.3% of NE participants (odds ratio 9.39, 95% CI: 3.55–24.83)]. In addition, WW participants reduced waist circumference by 3.9 ± 6.3 cm, while the NE group increased waist circumference by 0.6 ± 5.5 cm. Conclusions The WW program was associated with clinically significant weight loss, demonstrating its potential value as an intervention strategy, based in the community, for the treatment of obesity in China.

For the first time, ultralong hydroxyapatite (HAP) nanowires with lengths longer than 200 μm are used to fabricate highly porous HAP ceramics using palmitic acid spheres as pore formers. The effects of the aspect ratio of HAP and the addition amount of palmitic acid spheres on the formation of HAP porous ceramics are investigated and compared with commercial HAP nanoparticles. The ultralong HAP nanowires can cross-link with each other, leading to much stronger interaction among HAP nanowires than HAP nanoparticles. During the sintering process, although palmitic acid spheres are decomposed at high temperatures, ultralong HAP nanowires can be well preserved and still support the whole porous structure stably, leading to the formation of highly porous HAP ceramics with high biocompatibility. The porosity of the HAP nanowire porous ceramics can be easily tuned. The as-prepared HAP nanowire porous ceramics exhibit good biocompatibility, good performance for cell adhesion and spreading, and cell proliferation. Thus, the as-prepared HAP nanowire porous ceramics are promising for applications in various biomedical fields.

Wound dressings with pro-angiogenic activity are desirable for the rapid healing of full-thickness cutaneous wounds. It is well accepted that simvastatin can stimulate angiogenesis in addition to its lipid-lowering efficacy. However, the construction of a hydrogel-based wound dressing containing simvastatin remains a challenge due to its water-insolubility. In the present study, a novel wound dressing composed of alginate hydrogel (AH) and simvastatin-incorporated mesoporous hydroxyapatite microspheres (S-MHMs) was constructed for the sustained drug release of simvastatin. We first investigated the effect of simvastatin on the angiogenic differentiation of human umbilical vein endothelial cells (HUVECs). The in vitro results revealed that simvastatin significantly promoted the migration and tube formation of HUVECs. Furthermore, the activation of the Akt and Erk signaling pathways was detected in HUVECs upon treatment with simvastatin, and enhanced tube formation was reversed by LY294002 and PD98059, which indicated that both the Akt and Erk signaling pathways were involved in the process of angiogenesis induced by simvastatin. Moreover, simvastatin significantly up-regulated the expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF), which play essential roles in angiogenesis. For the in vivo experiments, simvastatin was incorporated into mesoporous hydroxyapatite microspheres (MHMs) synthesized using fructose 1,6-bisphosphate trisodium salt (FBP) as the phosphorous source by the microwave-assisted hydrothermal method. An simvastatin-loaded MHMs (S-MHMs) incorporated alginate hydrogel (S-MHMs/AH) was prepared as a wound dressing to promote the full-thickness cutaneous wound healing. The results demonstrated that S-MHMs/AH significantly enhanced new blood vessel formation and accelerated the reepithelialization of the cutaneous wounds. The present study suggests the potential application of the S-MHMs/AH composite as a novel dressing for wound healing.

In this paper, the one-step rapid synthesis of hydroxyapatite nanoflowers (HAFs) using creatine phosphate disodium salt as an organic phosphorus source by the sonochemical method is reported. The HAFs with diameters of about 300 nm are formed by self-assembly of hydroxyapatite nanosheets with thicknesses of less than 10 nm. The as-prepared samples are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET) nitrogen sorptometry, X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and inductively coupled plasma (ICP) optical emission spectroscopy. The MTT tests show that the as-prepared HAFs exhibit essentially inappreciable toxicity to MC-3T3 osteoblast cells, indicating that the HAFs have an excellent cytocompatibility. Moreover, the as-prepared HAFs show a relatively high protein adsorption ability when using hemoglobin (Hb) as a model protein. Thus, the as-prepared HAFs are promising for applications in various biomedical fields such as protein/drug delivery.

Continuous investigations of the roots of Aconitum nagarum var. lasiandrum led to the isolation of two new C₁₉-diterpenoid alkaloids, lasiandrine (1) and lasiandroline (2). Their structures were elucidated on the basis of extensive interpretation of spectroscopic and mass spectrometric data.

With the growing popularity of electrical communication equipment, high-performance electromagnetic interference (EMI) shielding materials are widely used to deal with radiation pollution. However, the large thickness and poor mechanical properties of many EMI shielding materials usually limit their applications. In this study, ultrathin and highly flexible Ti3C2Tx (d-Ti3C2Tx, MXene)/cellulose nanofiber (CNF) composite paper with a nacre-like lamellar structure is fabricated via a vacuum-filtration-induced self-assembly process. By the interaction between one-dimensional (1D) CNFs and two-dimensional (2D) d-Ti3C2Tx MXene, the binary strengthening and toughening of the nacre-like d-Ti3C2Tx/CNF composite paper has been successfully achieved, leading to high tensile strength (up to 135.4 MPa) and fracture strain (up to 16.7%), as well as excellent folding endurance (up to 14 260 times). Moreover, the d-Ti3C2Tx/CNF composite paper exhibits high electrical conductivity (up to 739.4 S m–1) and excellent specifi...

Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone. In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method. The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs). To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process. The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity. In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution. More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold. Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.

Hydroxyapatite (Ca 10 (OH) 2 (PO 4 ) 6 , HAP), as the major inorganic component of bones and teeth in vertebrates, is a very important biomaterial with high biocompatibility, nontoxicity and high thermal stability, thus, it has wide applications in various biomedical fields. Among various morphologies of HAP, HAP nanowires are very promising for various applications; however, the synthesis of ultralong HAP nanowires with lengths larger than 100 μm is highly challengeable and has been rarely reported. In this paper, we report, for the first time, the solvothermal synthesis of submillimeter-sized ultralong hydroxyapatite nanowires using calcium oleate as the precursor and NaH 2 PO 4 as the phosphorus source in a series of monohydroxy alcohols. The products are characterized by scanning electron microscopy, X-ray powder diffraction, and Fourier transform infrared (FTIR) spectroscopy. The as-prepared ultralong hydroxyapatite nanowires have diameters of tens of nanometers and lengths from several hundred micrometers up to nearly one millimeter. This method is simple, surfactant-free, environment friendly, and generally applicable in a series of monohydroxy alcohols including methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1-pentanol and 1-hexanol. Moreover, this method may be scaled up for large-scale production of ultralong hydroxyapatite nanowires. The as-prepared ultralong hydroxyapatite nanowires are promising for applications in various fields such as bone tissue engineering, drug delivery, adsorbent for organic pollutants and heavy metal ions, and nonflammable inorganic paper.

The synthesis of multifunctional biomaterials with antibacterial activity and remineralization effect on demineralized enamel remains a great challenge in the dental research field. Herein, we use chicken immunoglobulin Y (IgY) and amorphous calcium phosphate (ACP) nanospheres to prepare a nanocomposite with the multifunctional properties of antibacterial activity and remineralized effect. ACP nanospheres and La-ACP nanospheres with an amorphous structure are prepared by a simple coprecipitation method. Then, the as-prepared ACP nanospheres and La-ACP nanospheres which have high protein adsorption capacity are functionalized by adsorbing IgY molecules. After loading IgY, the ACP nanospheres and La-ACP nanospheres exhibit significant antibacterial activity against Sreptococcus mutans (S. mutans). Through the sustained release of IgY molecules and the mineralization of ACP nanospheres in simulated saliva fluid (SSF), the IgY-loaded ACP nanospheres have potential application in simultaneous inhibition of harmful oral bacteria and remineralization of demineralized tooth enamel. Due to the high biocompatibility, the as-prepared IgY-loaded ACP nanospheres and IgY-loaded La-ACP nanospheres are promising for prophylaxis and treatment of dental caries.

A rapid and simple strategy is reported for the synthesis of amorphous calcium phosphate (ACP) vesicle-like nanospheres using the adenosine 5'-triphosphate (ATP) disodium salt as a biocompatible phosphorus source and stabilizer by the sonochemical method in mixed solvents of water and ethylene glycol (EG). The ACP vesicle-like nanospheres are characterized by X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, UV/Vis absorption spectroscopy, thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), dynamic light scattering (DLS) and Brunauer-Emmett-Teller (BET) nitrogen adsorption. The ACP vesicle-like nanospheres exhibit essentially inappreciable toxicity to the cells in vitro. Furthermore, the as-prepared ACP vesicle-like nanospheres can be used as anticancer drug nanocarriers and show a pH-responsive drug release behaviour using doxorubicin (Dox) as a model drug. The ACP vesicle-like nanosphere drug delivery system exhibits a high ability to damage cancer cells, thus, it is promising for application in pH-responsive drug delivery.

Cognitive behavioral therapy, such as environmental enrichment combined with voluntary exercise (EE-VEx), is under active investigation as an adjunct to pharmaceutical treatment for chronic pain. However, the effectiveness and underlying mechanisms of EE-VEx remain unclear. In mice with intraplantar injection of complete Freund's adjuvant, our results revealed that EE-VEx alleviated perceptual, affective, and cognitive dimensions of chronic inflammatory pain. These effects of EE-VEx on chronic pain were contingent on the occurrence of adult neurogenesis in the dentate gyrus in a functionally dissociated manner along the dorsoventral axis: neurogenesis in the ventral dentate gyrus participated in alleviating perceptual and affective components of chronic pain by EE-VEx, whereas neurogenesis in the dorsal dentate gyrus was involved in EE-VEx's cognitive-enhancing effects. Chronic inflammatory pain was accompanied by decreased levels of brain-derived neurotrophic factor (BDNF) in the dentate gyrus, which were reversed by EE-VEx. Overexpression of BDNF in the dentate gyrus mimicked the effects of EE-VEx. Our results demonstrate distinct contribution of adult hippocampal neurogenesis along the dorsoventral axis to EE-VEx's beneficial effects on different dimensions of chronic pain.SIGNIFICANCE STATEMENTEnvironmental enrichment combined with voluntary exercise (EE-VEx) is under active investigation as an adjunct to pharmaceutical treatment for chronic pain, but its effectiveness and underlying mechanisms remain unclear. In a mouse model of inflammatory pain, the present study demonstrates that the beneficial effects of EE-VEx on chronic pain depend on adult neurogenesis with a dorsoventral dissociation along the hippocampal axis. Adult neurogenesis in the ventral dentate gyrus participates in alleviating perceptual and affective components of chronic pain by EE-VEx, whereas that in the dorsal pole is involved in EE-VEx's cognitive-enhancing effects in chronic pain.

To assess the prevalence of early childhood caries (ECC) in 2- to 5-year-old preschool children in Weifang City, China, and to investigate the factors related to this condition.A cross-sectional study was carried out among 2829 children (2-5 years old, including 1601 boys and 1228 girls) in twenty kindergartens in Weifang City, China, in 2014. These children were selected via cluster sampling. First, the percentages of ECC and severe ECC (S-ECC) as well as the mean dmft were compared between different genders and ages, and the tooth distribution pattern of ECC was analysed. Meanwhile, the information regarding feeding and oral hygiene practices was collected by a questionnaire with 14 questions to evaluate their relationships with the percentages of ECC and S-ECC as well as the mean dmft. The chi-squared test, t-test and one-way ANOVA were conducted to evaluate the significant differences.The ECC prevalence of the preschoolers was 53.3%, and the mean dmft was 2.12 ± 2.56. 71.4% of the 1509 children with ECC were identified as S-ECC. Not only the percentages of ECC and S-ECC but also the mean dmft differed statistically significantly according to gender and age (p 0.05). Furthermore, caries was most common in primary maxillary central incisors. Feeding and oral hygiene practices were shown to be the significant determining factors for caries.The oral health status of children living in Weifang is very poor. Appropriate strategies must be implemented to promote oral health early on by improving feeding and oral hygiene practices.

Hydroxyapatite (HAP) with a porous hollow structure is an ideal biomaterial owing to its excellent biocompatibility and unique architecture. In this study, HAP nanorod-assembled porous hollow polyhedra, consisting of nanorod building blocks, have been successfully prepared at room temperature or under hydrothermal circumstances using a self-sacrificing Ca(OH)2 template strategy. The hydrothermal treatment (at 180°C for 1h) can promote the HAP nanorods to be arranged with their axial direction normal to the polyhedron surface. The HAP nanorod-assembled porous hollow polyhedra have been explored for the potential application in drug/protein delivery, using ibuprofen (IBU) as a model drug and hemoglobin (Hb) as a model protein. The experimental results indicate that the HAP nanorod-assembled porous hollow polyhedra have a relatively high drug loading capacity and protein adsorption ability, and sustained drug and protein release. The HAP nanorod-assembled porous hollow polyhedra have promising applications in various biomedical fields such as the drug and protein delivery.

Background: Evidence for the effect of dietary sodium intake on the risk of cardiovascular disease has been controversial. One of the main explanations for the conflicting results lies in the great variability associated with measurement methods for sodium intake. Spot urine collection is a convenient method commonly used for sodium estimation, but its validity for predicting 24-h urinary sodium excretion at the individual level has not been well evaluated among the general population.Objective: The aim of this study was to evaluate the validity of the Kawasaki, the International Cooperative Study on Salt, Other Factors, and Blood Pressure (INTERSALT), and the Tanaka formulas in predicting 24-h urinary sodium excretion by using spot urine samples in Chinese adults.Design: We analyzed the relative and absolute differences and misclassification at the individual level from 3 commonly used methods for estimating sodium intake among 141 Chinese community residents.Results: The mean measured 24-h sodium excretion was 220.8 mmol/d. The median (95% CIs) differences between measured sodium and those estimated from the Kawasaki, INTERSALT, and Tanaka methods were 6.4 mmol/d (-17.5, 36.8 mmol/d), -67.3 mmol/d (-96.5, -46.9 mmol/d), and -42.9 mmol/d (-59.1, -24.8 mmol/d), respectively. The proportions of relative differences >40% with the Kawasaki, INTERSALT, and Tanaka methods were 31.2%, 41.1%, and 22.0%, respectively; and the absolute difference for the 3 methods was >51.3 mmol/d (3 g salt) in approximately half of the participants. The misclassification rate was 63.1% for the Kawasaki method, 78.7% for the INTERSALT method, and 66.0% for the Tanaka method at the individual level.Conclusion: The results from our study do not support the use of spot urine to estimate 24-h urinary sodium excretion at the individual level because of its poor performance with respect to misclassification. This trial was registered at www.chictr.org.cn as ChiCTR-IOR-16010278.

Cysteine (Cys) has unique chemical properties of catalysis, metal chelation, and protein stabilization. While Cys biosynthesis is assumed to be very ancient, the actual time of origin of these metabolic pathways remains unknown. Here, we use the molecular clocks of protein folds and fold superfamilies to time the origin of Cys biosynthesis. We find that the tRNA-dependent biosynthetic pathway appeared ~3.5 billion years ago while the tRNA-independent counterpart emerged ~500 million years later. A deep analysis of the origins of Cys biosynthesis in the context of emerging biochemistry uncovers some intriguing features of the planetary environment of early Earth. Results suggest that iron-sulfur (Fe-S) proteins that use cysteinyl sulfur to bind iron atoms were not the first to arise in evolution. Instead, their origin coincides with the appearance of the first Cys biosynthetic pathway. It is therefore likely that Cys did not play an important role in the make up of primordial protein molecules and that Fe-S clusters were not part of active sites at the beginning of biological history.

The ammonia-nitrogen wastewater was purified by using electrodialysis (ED) device in this paper. The NH4Cl solution was used as the artificial ammonia-nitrogen wastewater. Various unsteady factors in the experiment may cause the concentration polarization phenomenon, which may have negative effect on ED cell performance and its lifetime. The limiting voltage was confirmed as 12V in this study. At steady-state operation condition of ED, the outlet concentration was measured at different operation voltages, feed concentrations, and concentrations ratio of two cells. The efficiency of ammonia-nitrogen removal was evaluated in different conditions by a factorially designed experiment. The results from the experiment indicate the best process conditions for the ED, that is, the voltage was 12V, and the concentrations ratio of two cells was 1:3. It seems that the ammonia-nitrogen removal is more effective for a higher feed concentration stream.