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Additional file 1. Supplementary methods for tissue collection, histology and staining, immune profiling, molecular characterization, genomic profiling, and bioinformatic approaches.

Most knowledge on NK cells are based on studies of what are now known as conventional NK (cNK) cells in the mouse spleen or human peripheral blood. However, recent studies in mice indicate the presence of tissue-resident NK (trNK) cells in certain organs, such as the liver, that display different markers and transcription factor dependencies as compared to cNK cells. Here we provide evidence from cytometry by time-of-flight analysis and humanized mice indicating that human CD49e-negative NK cells are tissue-resident in the liver. Thus, these studies indicate that trNK cells are evolutionarily conserved in humans and mice, providing a foundation to explore their role in human disease.

Plasmodium gallinaceum de novo transcriptome assembly without quality trimming and no subsequent filtering of transcripts.

Genes and the corresponding GenBank Accession numbers and Gene IDs for the parasite taxa used in the study.

RSEM-eval score of various assemblies.

Plasmodium gallinaceum de novo transcriptome assembly without quality trimming and with subsequent filtering of transcripts.

Plasmodium gallinaceum de novo transcriptome assembly with quality trimming and with subsequent filtering of transcripts.

Pairwise comparisons of genetic distances, and the proportion of nonsynonymous differences per nonsynonymous site and synonymous differences per synonymous site between Plasmodium species.

Plasmodium gallinaceum de novo transcriptome assembly with quality trimming and no subsequent filtering of transcripts.